CAZyme Information: MGYG000004471_00008

Basic Information

• Species: UBA1394 sp900554975
• Lineage: Bacteria; Firmicutes_A; Clostridia; Oscillospirales; Ruminococcaceae; UBA1394; UBA1394 sp900554975
• CAZyme ID: MGYG000004471_00008
• CAZy Family: PL1
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
1160	MGYG000004471_1|CGC1	123836.85	4.481

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000004471	1997651	MAG	Israel	Asia

• Gene Location: Start: 7773; End: 11255 Strand: +

Full Sequence      

MKLKKLLSCFLASAIAAGGLVTGNYSFARIDSVTAASYISAPATSDVLRETAYITWTPVA
DADGYNVYVKPAGGAYTKLDTMLTRQYSGYFRADAVGLKAGKYVMKVVPTSGGKELSDKA
SESDTINVESYDRSGFAFSSNSPVKDGSIGAYNSDGTLKSDALVIYVTEDTKKTVTANIG
GTECTGIAAITQQAKKCKVPLCFRIIGQVTLDGISSADMSSAYAIGVKEASNVTFEGIGD
DATLYGAGVAAFKCNSVEIRNLGLMLWGGGKDGDGITLKTSKNVWVHNNDIFYGAKGSDP
DQVKGDGSMDLKDDSQYVTMSYNHFWDSGKMSLCGMKSETGENWITYHHNWFDHSDSRHP
RIRTMSVHVYNNYYDGNSKYGVGVTYGGNAFVEANYFRNCNYPVLVSMQGSDILSGETLS
GENGGVVKAYGNYMTGQKSYITQKTTTDKQDIDVYEASSRDEKVPADYTAKKGETSYNNF
DTNSDIMYSYTPDKAADVPAIVQAEAGRLGGGDFKYDFDDSKEDTDHDVNSELMSKLTSY
KTAVIAIGSGFTSGDTPVLTTAVTTAAPSTQTTVKTTVKATDASQSATTKATAATTKTSG
TAISIPVGDKTMYASPDGTGDGSSKDAPASLDKALAAIKANPGMTIYLLGGTYKLDSTIL
IEEANSGSAKAYNTIMAYPGETVVWDFSSMAVANSNRGVVLDGSYWHFKGFEIKGAGDNG
MLLSGDNNVIEMMVFNDNQDTGLQISRYQSAYNSIDQWPSNNLILNCTSKNNCDNATMEN
ADGFAAKLTCGEGNVFDGCMSYNNSDDGWDLFAKDATGPIGVVTIRNCIAFRNGYTESGE
GYGNCDGNGFKLGGSGVGTAHIVENCLAFENLNCGFTDNNNPKLGSLTNCTAYNNGVGGN
GKPNYSLYRCTDDGCDFSNIICYINQTELKNALAPNTSMKLANDKFVGTIQNSVYYNDKY
YSVTDKQNIANGNKIGTNVTLTDADFVSVSAPKMGTDFHTVWRNADGTINTNGFMQTVTK
GTYEKLGANFSAIDLPVVPVTSASTSASGSQTTETTVSTTVTTQETQPSTDVTSPSETVD
PSTLMYGDVDLDKVVGATDVVLINKYLLSTEEYPLTNEDNNRAYSQADASYDGDINLNDS
QAVIQKVLGIFTQADLGPQK

Enzyme Prediction     

No EC number prediction in MGYG000004471_00008.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
PL9	610	1020	1.8e-128	0.976
PL1	215	399	3.7e-47	0.8861386138613861

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
COG3866	PelB	1.19e-56	97	507	2	340	Pectate lyase [Carbohydrate transport and metabolism].
smart00656	Amb_all	3.37e-32	230	401	15	190	Amb_all domain.
pfam00544	Pec_lyase_C	1.86e-22	231	397	34	211	Pectate lyase. This enzyme forms a right handed beta helix structure. Pectate lyase is an enzyme involved in the maceration and soft rotting of plant tissue.
cd14256	Dockerin_I	3.31e-06	1086	1148	1	57	Type I dockerin repeat domain. Bacterial cohesin domains bind to a complementary protein domain named dockerin, and this interaction is required for the formation of the cellulosome, a cellulose-degrading complex. The cellulosome consists of scaffoldin, a noncatalytic scaffolding polypeptide, that comprises repeating cohesion modules and a single carbohydrate-binding module (CBM). Specific calcium-dependent interactions between cohesins and dockerins appear to be essential for cellulosome assembly. This subfamily represents type I dockerins, which are responsible for anchoring a variety of enzymatic domains to the complex.
pfam00404	Dockerin_1	7.54e-04	1087	1148	1	56	Dockerin type I repeat. The dockerin repeat is the binding partner of the cohesin domain pfam00963. The cohesin-dockerin interaction is the crucial interaction for complex formation in the cellulosome. The dockerin repeats, each bearing homology to the EF-hand calcium-binding loop bind calcium.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
AOR96287.1	1.58e-260	50	1013	65	1071
QMW93302.1	7.81e-258	50	1013	65	1071
BBK78741.1	7.81e-258	50	1013	65	1071
ABX41986.1	1.16e-255	50	1029	49	1157
ADL51369.1	5.12e-251	4	1032	16	1249

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
1RU4_A	1.82e-35	613	897	18	294	ChainA, Pectate lyase [Dickeya chrysanthemi]
1AIR_A	1.56e-21	238	412	94	272	ChainA, PECTATE LYASE C [Dickeya chrysanthemi],1O88_A Chain A, Pectate Lyase C [Dickeya chrysanthemi],1O8D_A Chain A, PECTATE LYASE C [Dickeya chrysanthemi],1O8E_A Chain A, PECTATE LYASE C [Dickeya chrysanthemi],1O8F_A Chain A, PECTATE LYASE C [Dickeya chrysanthemi],1O8G_A Chain A, PECTATE LYASE C [Dickeya chrysanthemi],1O8H_A Chain A, PECTATE LYASE C [Dickeya chrysanthemi],1O8I_A Chain A, PECTATE LYASE C [Dickeya chrysanthemi],1O8J_A Chain A, PECTATE LYASE C [Dickeya chrysanthemi],1O8K_A Chain A, PECTATE LYASE C [Dickeya chrysanthemi],1O8L_A Chain A, PECTATE LYASE C [Dickeya chrysanthemi],1O8M_A Chain A, PECTATE LYASE C [Dickeya chrysanthemi],1PLU_A Chain A, Protein (pectate Lyase C) [Dickeya chrysanthemi],2PEC_A Chain A, PECTATE LYASE C [Dickeya chrysanthemi]
3VMV_A	2.49e-21	229	399	77	248	Crystalstructure of pectate lyase Bsp165PelA from Bacillus sp. N165 [Bacillus sp. N16-5],3VMW_A Crystal structure of pectate lyase Bsp165PelA from Bacillus sp. N165 in complex with trigalacturonate [Bacillus sp. N16-5]
2EWE_A	3.79e-21	238	412	94	272	ChainA, Pectate lyase C [Dickeya chrysanthemi]
1VBL_A	2.12e-17	231	397	132	330	Structureof the thermostable pectate lyase PL 47 [Bacillus sp. TS-47]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
P0C1A6	1.60e-34	613	938	43	356	Pectate lyase L OS=Dickeya chrysanthemi OX=556 GN=pelL PE=3 SV=1
P0C1A7	1.60e-34	613	897	43	319	Pectate lyase L OS=Dickeya dadantii (strain 3937) OX=198628 GN=pelL PE=1 SV=1
P22751	5.39e-33	597	895	375	639	Pectate disaccharide-lyase OS=Dickeya chrysanthemi OX=556 GN=pelX PE=1 SV=1
Q8GCB2	2.21e-27	208	405	85	280	Pectate trisaccharide-lyase OS=Bacillus licheniformis OX=1402 GN=pelA PE=1 SV=1
B1B6T1	2.21e-27	208	405	85	280	Pectate trisaccharide-lyase OS=Bacillus sp. OX=1409 GN=pel PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.043451	0.947167	0.008372	0.000402	0.000289	0.000285

TMHMM  Annotations     

There is no transmembrane helices in MGYG000004471_00008.