CAZyme Information: MGYG000004424_00047

Basic Information

• Species: Collinsella sp900548365
• Lineage: Bacteria; Actinobacteriota; Coriobacteriia; Coriobacteriales; Coriobacteriaceae; Collinsella; Collinsella sp900548365
• CAZyme ID: MGYG000004424_00047
• CAZy Family: GH31
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
2398	MGYG000004424_1|CGC1	259383.73	4.1954

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000004424	2127341	MAG	Israel	Asia

• Gene Location: Start: 70167; End: 77363 Strand: -

Full Sequence      

MGYGIPKHWKMGGVGFVLATSLAAGWALNPALATPAFADEAMASDYQGTLTSATAAKKDA
DNDNIAYVTFNDNVTAKITFLEPGIFRYNVDLSGDFSAYATPRAKSHTAKIQAQPDTSGK
YSKPAATVNETKDAFEVSDGTVTLSFEKATGKMTLKRGDRVVFAEDEPLTITKSATTQSI
ADADADYFGGGTQNGRFIHTGKAINIKNESNWVDGGVSSPNPFYWSTAGYGVMRNTFAEG
KYDFGSAVAGSVDALHKDGEFDAYYFVSDDADTTASVAQDVLQEYYKVTGSPLLLPEYAF
YVGNYNAYNRDAWSHEPKSGYKEWSIHGHESASKAAPSKRYEKGGTGQTMLANSYVESLN
GTAPKDAETNENIPEGVQWSEDFSARAVVDEYQDMDMPFGYILPNDGYGCGYGQNGYQKT
GGVDKDGNSSAERLQAVADNVQNLKEFSDYAKSKGVATGLWTQSNLSPDSNANTQWQTLR
DFESEVKKGGVTTLKTDVAWVGSGYSFQLNGTKTAYDIVTTKNGDGTGARPNIVSLDGWA
GSQRFAGLWSGDQTGGNWEYIRFHIPTFIGSGLSGNPNIGSDMDGIFGGHPLIACRDYQW
KSFSSLMLDMDGWGSYAKMPYAYGDPYTGINRMYLKLKSSLLPYIYTTAASAANIDTGNG
DEGLPIVRAIALSDNSDIANSTATQYEYTLGEDLLIAPVYQNTDGDSANGGLGDGDDIRN
DIYLPGTSEDIWIDYWTGDQYRGGQVLNNFAVPLWKTPVFVKANAIIPMYKPNDNPSDID
RAQRDIEFFATDGENEYTLYEDDGSYVENKIDESDKEYGRESTISYGDHVSTKITSAVKD
GTATFTAAKSTGGYDGYDANRTTTFVVNVSAKPSELVAKNGDKTLELNEVASKEDFDKAE
GNVYFYNEAPNLNYNATAEDEAVRNEEFSKTEITTTPKLYVKFAKTDVTKDAQTLTLKGF
ENKGDLPANSLNENLAAPANLAAPEDDITPTSIKLTWDAVAGATGYELELDGVLSSVGDT
TTFTHANLAYNSSHTYRVRAVNADGYSAWSEPLTTKTALDPWRNVPVPVDWDFEGSEFGS
GYGVKFAFDHITGADASSFVSKETNGTGLALDLDYGKVYQFEKLEFRGSKYGQGVKQMKI
EASLDGTHWTDLGTHDLSGSFDKLNTVEFGAPVSARYIRMTAVQCTSYWNATEIAFYKVD
GINGAELGSINGDAVVDSADYQHLTGNCLGRENREPEAASYQTHVAKNGADFNQNGAYDV
YDMAFTMSKLDGGTTKTGDVSGGIAVMPSATHVEAGDTITVDVYASDAKNVNALGALVHF
KSDQFEFVKDSIEQSPYTSTMENLSIAKTEFDDGIQSVNLAFANKGDKELYDGSGVVASF
KLKAVAAGDVNLDSTAWLIGPTNDSIDVVSDGTIDWPEIPGEHEEEYAQSAFNCSILNEK
GEAVDVSKYIHQENFDGLFNGDVNSNDFEMEWQNAGDFDTSFHKLPATLRFEFKKPSALE
NVVVYNRTSGNGCVTELDASIVFEDGTKQDFTFGAKQNTFELAVSQENAGKKVARVDITP
KNTTTGINMLTLREIDFTYTTPGAQVAGVTVDDQTQTELYQGDLAQVFATVDCDDYPYFE
VSSDKPEVASVTAVQSGEGVDWYVRGNAEGTATITVAAKADPSKTATYEVTVRAGVDVSG
LQAIISEGRIYDSEAYTEASFAKLEAALKAAEDMLAQGQGSFTKNDVAQKSMDIENAIKG
LKMRPIDEAKLLNKDASSGMSVASVSSYAGESPMELALDYDEDTLWHSNYGSSMRLPQYI
VYDLGAEYDLTDVTFLPRQNGSLNGDIFKAQVYVADSVDELTAAADSGTLVGTFSFDNNG
KTLTNRNEYQQMAFGATTTRYVKINVIESGSSDGAGNRYCSIAETRFYGEKHTDPIGDAK
AELSTKVAEYEAKGLNADDYTASTWLPYANALADAKSAIEGDGLTAEQIAAVGERLDTAF
AGLKKTEIPPVVDADKEKLQAVVDLVKDTDLDGKTEATAKRFGDALKAAQQALKAGEGNW
KALYDELKAAYDGLADEAEVDTATLQGFVNMFDSLGMTSADFTADSWKAYADALAAAKDV
LAKDDATQEQVNACTDALTSAFKGLEFKQAPAAPYKGYLQKVVANFASEGLDESRYTADS
WKAYADALKAAQGVLDADDATQEQIDDATTALVEAHAGLQPAEKISFSDVDATVSHQDDI
VWLAANGISKGWENADGTFSFHPYENVARADMAAFLYRMAGEPEVDAEKAPSFTDVEKDT
PHYKAILWLAAEGISTGWENADGTAEFRPYAQITRADMAAFLYRMAGKPDVESPELGGFT
DVDEGTVHSDAILWLAAEGISTGWEHEDGTAEFRPYDQITRADMAAFLHRMDQKGLVK

Enzyme Prediction     

No EC number prediction in MGYG000004424_00047.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH31	511	767	1.3e-47	0.531615925058548

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
cd06596	GH31_CPE1046	5.66e-134	288	738	1	334	Clostridium CPE1046-like. CPE1046 is an uncharacterized Clostridium perfringens protein with a glycosyl hydrolase family 31 (GH31) domain. The domain architecture of CPE1046 and its orthologs includes a C-terminal fibronectin type 3 (FN3) domain and a coagulation factor 5/8 type C domain in addition to the GH31 domain. Enzymes of the GH31 family possess a wide range of different hydrolytic activities including alpha-glucosidase (glucoamylase and sucrase-isomaltase), alpha-xylosidase, 6-alpha-glucosyltransferase, 3-alpha-isomaltosyltransferase and alpha-1,4-glucan lyase. All GH31 enzymes cleave a terminal carbohydrate moiety from a substrate that varies considerably in size, depending on the enzyme, and may be either a starch or a glycoprotein.
COG1501	YicI	6.12e-51	222	815	195	719	Alpha-glucosidase, glycosyl hydrolase family GH31 [Carbohydrate transport and metabolism].
pfam01055	Glyco_hydro_31	1.26e-43	445	767	159	442	Glycosyl hydrolases family 31. Glycosyl hydrolases are key enzymes of carbohydrate metabolism. Family 31 comprises of enzymes that are, or similar to, alpha- galactosidases.
cd08759	Type_III_cohesin_like	2.18e-34	1282	1404	1	131	Cohesin domain, interaction partner of dockerin. Bacterial cohesin domains bind to a complementary protein domain named dockerin, and this interaction is required for the formation of the cellulosome, a cellulose-degrading complex. Two specific calcium-dependent interactions between cohesin and dockerin appear to be essential for cellulosome assembly, type I and type II. This subfamily represents type III cohesins and closely related domains.
cd06589	GH31	2.84e-30	384	635	25	265	glycosyl hydrolase family 31 (GH31). GH31 enzymes occur in prokaryotes, eukaryotes, and archaea with a wide range of hydrolytic activities, including alpha-glucosidase (glucoamylase and sucrase-isomaltase), alpha-xylosidase, 6-alpha-glucosyltransferase, 3-alpha-isomaltosyltransferase and alpha-1,4-glucan lyase. All GH31 enzymes cleave a terminal carbohydrate moiety from a substrate that varies considerably in size, depending on the enzyme, and may be either a starch or a glycoprotein. In most cases, the pyranose moiety recognized in subsite -1 of the substrate binding site is an alpha-D-glucose, though some GH31 family members show a preference for alpha-D-xylose. Several GH31 enzymes can accommodate both glucose and xylose and different levels of discrimination between the two have been observed. Most characterized GH31 enzymes are alpha-glucosidases. In mammals, GH31 members with alpha-glucosidase activity are implicated in at least three distinct biological processes. The lysosomal acid alpha-glucosidase (GAA) is essential for glycogen degradation and a deficiency or malfunction of this enzyme causes glycogen storage disease II, also known as Pompe disease. In the endoplasmic reticulum, alpha-glucosidase II catalyzes the second step in the N-linked oligosaccharide processing pathway that constitutes part of the quality control system for glycoprotein folding and maturation. The intestinal enzymes sucrase-isomaltase (SI) and maltase-glucoamylase (MGAM) play key roles in the final stage of carbohydrate digestion, making alpha-glucosidase inhibitors useful in the treatment of type 2 diabetes. GH31 alpha-glycosidases are retaining enzymes that cleave their substrates via an acid/base-catalyzed, double-displacement mechanism involving a covalent glycosyl-enzyme intermediate. Two aspartic acid residues have been identified as the catalytic nucleophile and the acid/base, respectively.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QWT17625.1	0.0	46	2083	46	2128
BCT46261.1	0.0	43	2131	36	2121
QNM10857.1	0.0	43	2029	37	1983
BBK61154.1	0.0	50	2118	39	2129
BBK23937.1	0.0	64	2071	69	2012

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
6M76_A	2.65e-222	68	1063	52	963	GH31alpha-N-acetylgalactosaminidase from Enterococcus faecalis [Enterococcus faecalis ATCC 10100],6M77_A GH31 alpha-N-acetylgalactosaminidase from Enterococcus faecalis in complex with N-acetylgalactosamine [Enterococcus faecalis ATCC 10100]
7F7R_A	1.37e-221	68	1063	52	963	ChainA, GH31 alpha-N-acetylgalactosaminidase [Enterococcus faecalis ATCC 10100]
7F7Q_A	3.66e-221	68	1063	52	963	ChainA, GH31 alpha-N-acetylgalactosaminidase [Enterococcus faecalis ATCC 10100]
5F7C_A	1.19e-17	513	778	488	726	Crystalstructure of Family 31 alpha-glucosidase (BT_0339) from Bacteroides thetaiotaomicron [Bacteroides thetaiotaomicron VPI-5482],5F7C_B Crystal structure of Family 31 alpha-glucosidase (BT_0339) from Bacteroides thetaiotaomicron [Bacteroides thetaiotaomicron VPI-5482],5F7C_C Crystal structure of Family 31 alpha-glucosidase (BT_0339) from Bacteroides thetaiotaomicron [Bacteroides thetaiotaomicron VPI-5482],5F7C_D Crystal structure of Family 31 alpha-glucosidase (BT_0339) from Bacteroides thetaiotaomicron [Bacteroides thetaiotaomicron VPI-5482]
7KBJ_A	2.17e-15	483	770	198	466	ChainA, Neutral alpha-glucosidase AB Trypsin-cleaved Fragment #3 [Mus musculus],7KBJ_C Chain C, Neutral alpha-glucosidase AB Trypsin-cleaved Fragment #3 [Mus musculus],7KBR_A Chain A, Neutral alpha-glucosidase AB Trypsin-cleaved Fragment #3 [Mus musculus],7KBR_C Chain C, Neutral alpha-glucosidase AB Trypsin-cleaved Fragment #3 [Mus musculus],7L9E_A Chain A, Neutral alpha-glucosidase AB Trypsin-cleaved Fragment #3 [Mus musculus],7L9E_C Chain C, Neutral alpha-glucosidase AB Trypsin-cleaved Fragment #3 [Mus musculus]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
Q9P999	3.22e-21	501	804	376	658	Alpha-xylosidase OS=Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2) OX=273057 GN=xylS PE=1 SV=1
Q9F234	1.27e-19	528	804	460	711	Alpha-glucosidase 2 OS=Bacillus thermoamyloliquefaciens OX=1425 PE=3 SV=1
B9F676	1.99e-17	483	768	513	778	Probable glucan 1,3-alpha-glucosidase OS=Oryza sativa subsp. japonica OX=39947 GN=Os03g0216600 PE=3 SV=1
P79403	7.91e-17	483	770	545	813	Neutral alpha-glucosidase AB OS=Sus scrofa OX=9823 GN=GANAB PE=1 SV=1
Q4R4N7	9.09e-16	483	770	545	813	Neutral alpha-glucosidase AB OS=Macaca fascicularis OX=9541 GN=GANAB PE=2 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.077516	0.919526	0.001749	0.000511	0.000343	0.000331

TMHMM  Annotations     

start	end
12	34