CAZyme Information: MGYG000004410_01070

Basic Information

• Species: Methanosphaera cuniculi
• Lineage: Archaea; Methanobacteriota; Methanobacteria; Methanobacteriales; Methanobacteriaceae; Methanosphaera; Methanosphaera cuniculi
• CAZyme ID: MGYG000004410_01070
• CAZy Family: GT2
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
1519		178263.97	5.2221

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000004410	1661817	MAG	Israel	Asia

• Gene Location: Start: 694; End: 5253 Strand: -

Full Sequence      

MGFVESANRGIREANENDVVILTSDTIVTPRWLSKLVIGAYSYEEIATITPLSNNSDLNA
TYLGRDDDQLFLNKNSYQLEKLDYNSNQLAPIGNGICFYIKSGAIKKLGDLNTSFKRGYG
EAIDFSAYAYEKGWLNMRSFDTFIYHQGGASFSPDETKKLTKEHEKLNQQKHPQTLKEWN
KFKNSSRVKDLLNKTKKTQSYGNKERLLYITGFDGNGNPEITPEYYKMAQEYETHVLALD
KKGISLYVYDGIFKFRKIYQNDVMEEDSAQIKLIYFNLLSMLKYNLLYIRPIFYYASHVF
LEMTQFIKIAKPLEIPIVYEGNDFYEDLMTHINYKLNPIETFDETITKAQNRLDLKDKKV
AVYTAITGGYDVLETPSIENRNFDYICFTDNPNLTSDFWEIRLMEDEEELDAIRRARKYK
VLPHKYLPEYDYSLWIDGSFDIISNIADYIQDYSQNHKLLAIPHEVRDCIYEEAEVCIEK
EKDSPEIINEQMAKYEEEGYPQHNGLIASGILFRDHHDPEVIKLMEMWFDEIRNYSRRDQ
LSFNYVCWKNNFQYDTSDIFYHRNQYFSRLIHGITEEVRNYGWGIKKRVLSNIQNPTTII
MPVYNAYEDTKKCIKSVLKYTNIPYELLLIDDNSPDPRINPMLREFSEKYDHIKLITNKK
NMGFVANVNLGILSTKNDVVLLNSDTIVTPNWLRKLKVTAYESWNIGTVTPLSNNAGAFS
VPLIGQKNDIDAKIGLEGTANIIEKLGKRSIEIPTGHGFCMYIRREVIDEVGLFDLIFNM
GYCEENDFSMRVINAGWKNVLDPTTYIYHNQGSSFGKKRDERIRRNREILDLKHPDYKER
IDEFLEDKEFLEIREDIGDALADKKIVDNNKKNILYVIHESKGGTPYTTNDLMQHVIDDY
NIYMLTAGRFFIRLYKFVPVKLKNSKGLENGAEFRKDLFQFYKWEIKSYYTISNLYNEEF
EQRYFNVLLGLHIDVVHIRHLIRHTFDLPRIAHALGIKVILSFHDLYYICPSHNLIDDRN
QYCGGHCTPLIPKRERSNCSVIKGINEPENLRDFLPTWRKNVSEMYKYCDEFVTTSQSTY
DLYTEFYPELKEKPFHIIEHGRDLKTPDVIDITPEQIDPQEPIKIVIPGNMNVGKGINFI
IKLKEQDVDNRLEFHFMGNAYYDFRYNKLGIQHGQYKRSEFNDIVESIDPMFIGIFSIWP
ETYCHTLTEAWSTGVPVLTMDIGALGERVKNNGGGYIVSDDPKEAYEQIINFVENKDEYV
QVRKQIEDIKFPSTRDMADAYLKIYEDEEKPSILITGLKEDYVNSNMTEDLIFELADYYD
IYALTSNKESIKLYKYNQLQPDYINKLPDHDFMDDYEYVKRWNIRYIEKSENLKQQQYKT
IAEELRDKFNIKLIQVEDFDENINQVLENVIDDNEIKLVTSINTDKYLPKHENTTTTDNL
SFTQKIRQEQQIQTQDVQKRKKDTDETTTSIDPIISKIIQDSSELIFKSEELYNKYLNAY
PQISNIKSKIIEDRIKVKT

Enzyme Prediction     

No EC number prediction in MGYG000004410_01070.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GT2	598	771	3.2e-21	0.9882352941176471

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
cd04186	GT_2_like_c	9.08e-38	599	809	1	164	Subfamily of Glycosyltransferase Family GT2 of unknown function. GT-2 includes diverse families of glycosyltransferases with a common GT-A type structural fold, which has two tightly associated beta/alpha/beta domains that tend to form a continuous central sheet of at least eight beta-strands. These are enzymes that catalyze the transfer of sugar moieties from activated donor molecules to specific acceptor molecules, forming glycosidic bonds. Glycosyltransferases have been classified into more than 90 distinct sequence based families.
COG1216	GT2	6.13e-32	597	841	5	254	Glycosyltransferase, GT2 family [Carbohydrate transport and metabolism].
cd03823	GT4_ExpE7-like	1.61e-22	949	1287	73	357	glycosyltransferase ExpE7 and similar proteins. This family is most closely related to the GT4 family of glycosyltransferases. ExpE7 in Sinorhizobium meliloti has been shown to be involved in the biosynthesis of galactoglucans (exopolysaccharide II).
pfam00535	Glycos_transf_2	1.96e-22	598	768	1	164	Glycosyl transferase family 2. Diverse family, transferring sugar from UDP-glucose, UDP-N-acetyl- galactosamine, GDP-mannose or CDP-abequose, to a range of substrates including cellulose, dolichol phosphate and teichoic acids.
cd00761	Glyco_tranf_GTA_type	9.95e-19	599	714	1	116	Glycosyltransferase family A (GT-A) includes diverse families of glycosyl transferases with a common GT-A type structural fold. Glycosyltransferases (GTs) are enzymes that synthesize oligosaccharides, polysaccharides, and glycoconjugates by transferring the sugar moiety from an activated nucleotide-sugar donor to an acceptor molecule, which may be a growing oligosaccharide, a lipid, or a protein. Based on the stereochemistry of the donor and acceptor molecules, GTs are classified as either retaining or inverting enzymes. To date, all GT structures adopt one of two possible folds, termed GT-A fold and GT-B fold. This hierarchy includes diverse families of glycosyl transferases with a common GT-A type structural fold, which has two tightly associated beta/alpha/beta domains that tend to form a continuous central sheet of at least eight beta-strands. The majority of the proteins in this superfamily are Glycosyltransferase family 2 (GT-2) proteins. But it also includes families GT-43, GT-6, GT-8, GT13 and GT-7; which are evolutionarily related to GT-2 and share structure similarities.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
ABC56892.1	0.0	2	1286	216	1496
AWX32257.1	0.0	2	1286	918	2210
AWX32258.1	1.10e-270	18	1287	921	2176
AUB55815.1	2.27e-197	598	1285	579	1275
BAZ45973.1	1.06e-143	592	1286	846	1534

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
3BCV_A	9.57e-06	598	688	8	97	Crystalstructure of a putative glycosyltransferase from Bacteroides fragilis [Bacteroides fragilis NCTC 9343],3BCV_B Crystal structure of a putative glycosyltransferase from Bacteroides fragilis [Bacteroides fragilis NCTC 9343]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
P11290	1.10e-06	590	702	1	112	Uncharacterized glycosyltransferase YibD OS=Escherichia coli (strain K12) OX=83333 GN=yibD PE=3 SV=2
Q57287	1.74e-06	598	696	8	106	Uncharacterized glycosyltransferase HI_1578 OS=Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd) OX=71421 GN=HI_1578 PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000058	0.000001	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

There is no transmembrane helices in MGYG000004410_01070.