CAZyme Information: MGYG000004369_01498

Basic Information

• Species: CAG-882 sp000435595
• Lineage: Bacteria; Firmicutes_A; Clostridia; Lachnospirales; Lachnospiraceae; CAG-882; CAG-882 sp000435595
• CAZyme ID: MGYG000004369_01498
• CAZy Family: GH2
• CAZyme Description: Beta-galactosidase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
1159	MGYG000004369_18|CGC1	130427.36	4.7059

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000004369	3090795	MAG	Israel	Asia

• Gene Location: Start: 40031; End: 43510 Strand: -

Full Sequence      

MNKTVLWDDGWCFKKTSLGVTLDEALDEKFNAVDIPHDWLIYDTNNLYESSIGWYKKKFS
VSKEELCKKLIVRFGAVYMDSTVYVNGRAVCEWKYGYSTFGADITEYVHEGDNEIVVKAV
YQCPNTRWYSGAGIIRDVYFMTLEPLHVVPDGTYVRVRKNDDGTWHVKCSVEIENSGHDI
SAGDVQAGLTLSHAGSSVFTFGRMAGSEAAVFESGAKGSVELEITIDKPLIWDIDEPNLY
ECSVSLKYKGEVQHEESQKIGFCTKEYSPENGFILNGRKVRLNGVCEHHDNGCLGAAFNV
NAFRRKLNKLRKMGVNAVRTSHNMPAKEVLELADEMGFLVDCEAFDMWELPKTEFDYARF
FVDWADKDVASWIRRDRNHVSVIMWSIGNEIYDTHRDEHGQEITRRLTGYVREHDPECNA
PVTIGSNYMPWENAQKCADIVKLVGYNYAEKYYEQHHKEHPDWIIYGSETASTLQSRGIY
KFPYEQRILCDDDEQCSSLGNCSTNWGAKNTEYCITMDRDAHYSMGQFIWTGFDYIGEPT
PYQTKNSYFGQLDTAGFEKDTYYIYQAEWTDYKKAPMVHVLPYWDFSEGEVVDVRVASNA
PKVELFLNGRSLGTYDIDHEHGKELLAHYKVEYEPGVVEAVAYDESGSVIARDVKKSFSD
AVKLIVTPECTSIKADGRELVYVDISAVDKDGNPVENANNRVYVKVSGAGRLVGMDNGDS
ADFDQYKTDSRRMFSGKLLAVIAAKTEPGEIKLEVSAEGMDTVAVPMEALACGKMTGVSA
GENISAMDEADADIEIPVRNIRMKVKGSRVLSPDNTQVEIEAELLPHNATYKELIWKVTN
DAGVESNGAVLEDGEVSEVPYIVKKVLKAVGDGKHNVRCCTRNGREKISLISQISLTAEG
LGMAEFNPYEFVYGTLYTSAEVEPAVGQDRGIVPKGGTYIAFEKVNFGKNGSDEITLPVY
ELNNKPFPIQIWKGIPGHDGAELVDTVIYDLPSIWATYQEKTYELPKRFKGVCTVSFVAD
HDINIKGFSFKKYERAYDINYAVDNDGIYGDTYTLGADSITSIGNNVALTYNDMNFTHGM
HKITICGRSVLEKNTIHVRFANEEGTENRIAEFIMPHTVESGDGYVEQSFELENIEGSYS
VNLVFLPGCNFDLKWFRFE

Enzyme Prediction     

EC	3.2.1.23

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH2	8	570	4.9e-100	0.5944148936170213

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
COG3250	LacZ	1.05e-61	33	720	63	766	Beta-galactosidase/beta-glucuronidase [Carbohydrate transport and metabolism].
pfam18565	Glyco_hydro2_C5	1.32e-36	666	765	3	102	Glycoside hydrolase family 2 C-terminal domain 5. Domain 5 is found in dimeric beta-D-galactosidase from Paracoccus sp. 32d, which contributes to stabilization of the functional dimer. It is suggested that the location of this domain 5, may be one of the factors responsible for the creation of a functional dimer and cold-adaptation of this enzyme.
PRK10150	PRK10150	3.49e-32	54	453	68	473	beta-D-glucuronidase; Provisional
PRK10340	ebgA	1.23e-31	53	390	111	449	cryptic beta-D-galactosidase subunit alpha; Reviewed
PRK09525	lacZ	4.12e-21	34	390	97	462	beta-galactosidase.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QOS77051.1	0.0	1	1159	1	1168
AGA58497.1	0.0	1	1159	1	1140
SQI53931.1	0.0	1	1158	1	1140
APO44079.1	0.0	1	1159	1	1156
QLG36749.1	0.0	1	1159	1	1158

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
7CWD_A	2.26e-166	3	762	2	794	ChainA, beta-glalactosidase [Niallia circulans],7CWI_A Chain A, beta-galactosidase [Niallia circulans]
4YPJ_A	6.65e-163	2	762	7	800	ChainA, Beta galactosidase [Niallia circulans],4YPJ_B Chain B, Beta galactosidase [Niallia circulans]
4CU6_A	1.15e-149	2	753	13	828	Unravellingthe multiple functions of the architecturally intricate Streptococcus pneumoniae beta-galactosidase, BgaA [Streptococcus pneumoniae TIGR4],4CU7_A Unravelling the multiple functions of the architecturally intricate Streptococcus pneumoniae beta-galactosidase, BgaA [Streptococcus pneumoniae TIGR4],4CU8_A Unravelling the multiple functions of the architecturally intricate Streptococcus pneumoniae beta-galactosidase, BgaA [Streptococcus pneumoniae TIGR4]
4CUC_A	3.17e-149	2	753	13	828	Unravellingthe multiple functions of the architecturally intricate Streptococcus pneumoniae beta-galactosidase, BgaA. [Streptococcus pneumoniae TIGR4]
6QUB_B	3.73e-134	2	771	7	855	Truncatedbeta-galactosidase III from Bifidobacterium bifidum in complex with galactose [Bifidobacterium bifidum]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
A7LXS9	3.23e-150	27	769	68	848	Beta-galactosidase BoGH2A OS=Bacteroides ovatus (strain ATCC 8483 / DSM 1896 / JCM 5824 / BCRC 10623 / CCUG 4943 / NCTC 11153) OX=411476 GN=BACOVA_02645 PE=1 SV=1
T2KM09	2.70e-91	7	757	51	808	Putative beta-glucuronidase OS=Formosa agariphila (strain DSM 15362 / KCTC 12365 / LMG 23005 / KMM 3901 / M-2Alg 35-1) OX=1347342 GN=BN863_22050 PE=2 SV=2
T2KPJ7	2.48e-50	27	713	76	775	Putative beta-glucuronidase OS=Formosa agariphila (strain DSM 15362 / KCTC 12365 / LMG 23005 / KMM 3901 / M-2Alg 35-1) OX=1347342 GN=BN863_21970 PE=2 SV=1
P77989	1.11e-37	1	714	1	695	Beta-galactosidase OS=Thermoanaerobacter pseudethanolicus (strain ATCC 33223 / 39E) OX=340099 GN=lacZ PE=3 SV=2
T2KN75	1.08e-32	27	708	52	736	Beta-glucuronidase OS=Formosa agariphila (strain DSM 15362 / KCTC 12365 / LMG 23005 / KMM 3901 / M-2Alg 35-1) OX=1347342 GN=BN863_22060 PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000042	0.000000	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

There is no transmembrane helices in MGYG000004369_01498.