CAZyme Information: MGYG000004355_00100

Basic Information

• Species: CAG-302 sp900554615
• Lineage: Bacteria; Firmicutes; Bacilli; RF39; UBA660; CAG-302; CAG-302 sp900554615
• CAZyme ID: MGYG000004355_00100
• CAZy Family: GT51
• CAZyme Description: Penicillin-binding protein 2D

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
661		75336.67	6.1725

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000004355	1353414	MAG	Israel	Asia

• Gene Location: Start: 22431; End: 24416 Strand: +

Full Sequence      

MKKNKKKIILLSLLPLMLFISVHIVVFTYAYITPKLEINKSKSYYLYDTKNELVFNNDKD
WVSLQEISPYLIEATLSTEDKHFYKHIGFDYLRISKALINNIIKGDKSEGASTISQQYAR
NLFLNFEKTWKRKIDEALLATELEVHYTKDEILEGYLNTINYGGVYGIEAASKYYFDKSA
KDLTLEEASMLAGIPKSPNNYSPVKNYSKAKERQKIVLKLMKDNKKITEEEYNTAINKNL
QIIGKKEKNNITSINYFRDSVLEEIKKLTNIPEEITKTGGLKIYTTFDIEAQEDLEKAVY
SYVNDETEVETAAIMMNPNTGGVMAMIGGNSYYKSEYNRATKAKRQVGSTMKPLLYYTAL
ESGFTASSSFTSEKTTFTFAGDKTYSPNNYNNTYANSPISMAAAISYSDNIYAVKTHLFL
GENMLINTAKRLGITSNLTEIPSLALGTEEISLLEMTTSYSAFANLGYKVEPHFIKKIVD
SKGNVLYENKEEKELILNQSLTFILNEMLTYTYNKNFIDYNYPTLISLLPSITHKYSIKS
GTTDTDLLIMGYNKDAVLGIWNGYDNNKKIESKDYSYHKNIWIDTMEAYFKDKETTWYDI
PSNVVGVLVNPITGIIANDNDTKKEMFFYIKGTEPTLNGTTKDLDAVFKEENSSNEQKEA
P

Enzyme Prediction     

No EC number prediction in MGYG000004355_00100.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GT51	55	222	1.1e-64	0.9491525423728814

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
COG0744	MrcB	0.0	24	610	29	620	Membrane carboxypeptidase (penicillin-binding protein) [Cell wall/membrane/envelope biogenesis].
COG5009	MrcA	3.66e-137	62	657	70	792	Membrane carboxypeptidase/penicillin-binding protein [Cell wall/membrane/envelope biogenesis].
pfam00912	Transgly	1.00e-82	60	222	14	177	Transglycosylase. The penicillin-binding proteins are bifunctional proteins consisting of transglycosylase and transpeptidase in the N- and C-terminus respectively. The transglycosylase domain catalyzes the polymerization of murein glycan chains.
PRK11636	mrcA	6.74e-76	62	636	69	829	penicillin-binding protein 1a; Provisional
COG4953	PbpC	1.60e-72	61	568	60	539	Membrane carboxypeptidase/penicillin-binding protein PbpC [Cell wall/membrane/envelope biogenesis].

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QVK17409.1	1.07e-181	34	635	44	649
QNG60090.1	2.90e-178	61	636	91	669
AYA78204.1	5.43e-178	34	636	47	654
QOR66557.1	3.17e-177	61	636	76	654
AIE61597.1	4.08e-177	3	661	14	678

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
4OON_A	1.40e-54	62	614	42	747	Crystalstructure of PBP1a in complex with compound 17 ((4Z,8S,11E,14S)-5-(2-amino-1,3-thiazol-4-yl)-14-(5,6-dihydroxy-1,3-dioxo-1,3-dihydro-2H-isoindol-2-yl)-8-formyl-2-methyl-6-oxo-3,10-dioxa-4,7,11-triazatetradeca-4,11-diene-2,12,14-tricarboxylic acid) [Pseudomonas aeruginosa PAO1]
5U2G_A	2.08e-54	62	636	43	801	2.6Angstrom Resolution Crystal Structure of Penicillin-Binding Protein 1A from Haemophilus influenzae [Haemophilus influenzae Rd KW20],5U2G_B 2.6 Angstrom Resolution Crystal Structure of Penicillin-Binding Protein 1A from Haemophilus influenzae [Haemophilus influenzae Rd KW20]
5FGZ_A	3.58e-53	71	568	168	672	E.coli PBP1b in complex with FPI-1465 [Escherichia coli K-12],5HL9_A E. coli PBP1b in complex with acyl-ampicillin and moenomycin [Escherichia coli K-12],5HLA_A E. coli PBP1b in complex with acyl-cephalexin and moenomycin [Escherichia coli K-12],5HLB_A E. coli PBP1b in complex with acyl-aztreonam and moenomycin [Escherichia coli K-12],5HLD_A E. coli PBP1b in complex with acyl-CENTA and moenomycin [Escherichia coli K-12],6YN0_A Structure of E. coli PBP1b with a FtsN peptide activating transglycosylase activity [Escherichia coli K-12],7LQ6_A Chain A, Penicillin-binding protein 1B [Escherichia coli K-12]
3VMA_A	4.47e-53	71	568	189	693	CrystalStructure of the Full-Length Transglycosylase PBP1b from Escherichia coli [Escherichia coli K-12]
3UDF_A	1.42e-52	62	492	42	564	ChainA, Penicillin-binding protein 1a [Acinetobacter baumannii],3UDF_B Chain B, Penicillin-binding protein 1a [Acinetobacter baumannii],3UDI_A Chain A, Penicillin-binding protein 1a [Acinetobacter baumannii],3UDI_B Chain B, Penicillin-binding protein 1a [Acinetobacter baumannii],3UDX_A Chain A, Penicillin-binding protein 1a [Acinetobacter baumannii],3UDX_B Chain B, Penicillin-binding protein 1a [Acinetobacter baumannii],3UE0_A Chain A, Penicillin-binding protein 1a [Acinetobacter baumannii],3UE0_B Chain B, Penicillin-binding protein 1a [Acinetobacter baumannii],3UE1_A Chain A, Penicillin-binding protein 1a [Acinetobacter baumannii],3UE1_B Chain B, Penicillin-binding protein 1a [Acinetobacter baumannii]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
P70997	1.90e-162	34	635	47	653	Penicillin-binding protein 2D OS=Bacillus subtilis (strain 168) OX=224308 GN=pbpG PE=2 SV=3
O66874	1.74e-82	46	647	47	722	Penicillin-binding protein 1A OS=Aquifex aeolicus (strain VF5) OX=224324 GN=mrcA PE=1 SV=1
P38050	3.91e-72	54	565	62	571	Penicillin-binding protein 1F OS=Bacillus subtilis (strain 168) OX=224308 GN=pbpF PE=2 SV=2
P39793	7.56e-66	34	651	64	705	Penicillin-binding protein 1A/1B OS=Bacillus subtilis (strain 168) OX=224308 GN=ponA PE=1 SV=1
O87579	3.95e-63	59	605	68	721	Penicillin-binding protein 1A OS=Neisseria lactamica OX=486 GN=mrcA PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.723780	0.252174	0.005094	0.002392	0.001247	0.015325

TMHMM  Annotations     

start	end
9	31