CAZyme Information: MGYG000004292_01549

Basic Information

• Species: UMGS1474 sp900552115
• Lineage: Bacteria; Firmicutes_A; Clostridia; Oscillospirales; Acutalibacteraceae; UMGS1474; UMGS1474 sp900552115
• CAZyme ID: MGYG000004292_01549
• CAZy Family: PL8
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
2091	MGYG000004292_67|CGC1	225904.41	4.4522

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000004292	2231746	MAG	China	Asia

• Gene Location: Start: 4280; End: 10555 Strand: +

Full Sequence      

MKRYARKWTSLLLACAMMLSMIPFGILAATAQEQAFDVPQDYTLSPVATAEELAAVATGK
EASKVYYYLTNDITIEADSFKGIGTNENTDAFWDILDGNGYAIDFAGPEGKGVALNGSGA
WNGGLFFSMNGDAVVKNLTLKGTITSTAQSTGALVGHMVNGTIERCVNRASVTAAGKEGV
GGLAGRVTRGIVVNCMNYGAITGGNLTGGLVGKASYATKDINAVSIANGANFGSVTGKEK
VGGILGLGQADRTYTIENCYNVGNISGTTAATQGAIACSNWQSAVKTVNCYALDTSCKKF
LNATIKMSASDMQAQAFADTLNANVEAGIVFADGVANTEVTLPALKWQYNEGGYPTIPMV
EPVVPEKDLTLEEQMDVIRQRITEYFLRNDAVHSSGAHNGICYVSKAGEYLEMQNEDGSW
SDVDYYCTQSAANGAVWEPYLALDRMLAMSYAWAKEGGEYYHSQEMADGVNKAFAYWASI
KDANPDNPNWVGPWSTNWWENSNGVPRRFGPIGVVMKEGLTKESMDLILGRVNKEGDAGS
GQNPLWHTQNALYWSLAAGDAAQLKKVVEKYLSVNLRVGGLNDEALQVDNTFHCHGNQLY
TNGYGKSLFSDMALWIDLLAGTDYAVPQHVLDLMADYMLGGTRWMIRGDLLELADGYKGS
GKNASGYIEPLQRMVKNDPKNAAEYQKVLDSITGASGTTYNGANGSNYMWTSALMSHMRE
GYGVNVRMNHKGMKSTEWRATWPKTDFGNLIFWTADATASIMIDGDEYNSVYNTYDWRHV
PGVTAPFVLATHYGFDNDSNDCWGVTNGTYGATAYTFNKHDGTNKRTQGKVGYFFFDDEY
VALGAGIRANHEQAIHTTLNQTKAADVSVNGTAVADGADDEVYQAEYVYNNQIGYVFPQQ
TEVHLSNLNHTPKKYPTVRGNGYSDLSEDRFGETEESTFSLWLDHGVQPQDASYAYIVVP
NTTEEELAAYNKNNQITIVTNTERVQAVRHEGLKQTQINFYEAGTLEYAEGKTVSVDGPC
SLIIDETGRDPIISEAVSNTRPNTMVNVVLTTSDANTVTEFCSLSEPYAGKSITQSAGGS
SLMQSSATTPEHGAAKAFDKNLETYWESDEAPAWICYDMREDLYVGSMTIRWGEAYATKY
ELQYSEDGVNWQTAYAQETGKGGTETVSFNTISRYWRLWCTESAGDTIQIKEISFQSSRN
LATNKPMTASFSADGKPASNAVDGDMTTRWVGEKGKKDGWITADLGMNTRIDGVRISWET
SYASKYTIDVSDDGETWKTAATVTAPAVSGEKIVTVQTALPQGTTGRYLRVQGVEMALPQ
YGLSIWEMEIYGSLQIDSDNVAAGKTAVDSAANIVDAVTDGNVTTVWSSGTFPASLTIDL
GEMHQIDSVAAVWGDRFASAYKWQISADGETFSDAADVTAGQGGRETADMAINARYLRLL
VTDAAGGVDIAELEVYGTALSQMNPDKDSLKKAMDTPVRNDVYTEASYAAYQSALSVAVN
VYSSPNVTQKMVDEALADLLEAIDALTFRNFGTVLATIPGQSVSCGSRQVLGTNWTNLAS
ALDLSQENLDKIYLFATVDITRDPDREEAGMFGNGRIMLRSPDAPTENNVFINTNTQITH
LGRTVLYFPLSEMTGKTGTMDWSQVERFRIYIDSVNKFEGKMTMTLSDMQIIRTTEPVKV
KVACVGDSITAGSGASSASNNYVSQLQQRLGTHYTVQNFGNSGKTLLKDGADNSGYTKTD
TFANSKAFAPDVVTIMLGTNDSKDATWNAHGDKYEQELRELIKVYRDLPSHPTVILATSP
TAYNRNWNINDAVVSGEIAPIQRRVAAEMGCPLIDANTATKNAADKFADGIHPNDAGYVL
LADLFAEGITNAATTVYSFSLAGQAGTIDTENGTIAVTVPYDTDLTALIPDIGLMTGATL
SPAGAADFSSPVTYTVTAPDQKTTRMYTVTVTKEAQRVNKDDLKKALDEQITDLTPYTEA
TAAAYTAALNAAQTVYSDPQATQKQVDDALADLMEAKDGLEHKPAVVLGDINGDGHINAA
DALLALQAATHKITLTPAQAAAADVDGKDGVTAGDALLILQFATQKISSFK

Enzyme Prediction     

No EC number prediction in MGYG000004292_01549.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
PL8	708	961	5.1e-45	0.9839357429718876
CBM32	1208	1329	5e-25	0.9274193548387096
CBM32	1084	1193	9.3e-22	0.9112903225806451
CE3	1680	1869	1.7e-19	0.9948453608247423
CBM32	1350	1453	2.8e-16	0.8709677419354839

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
cd01083	GAG_Lyase	6.19e-83	376	1034	3	689	Glycosaminoglycan (GAG) polysaccharide lyase family. This family consists of a group of secreted bacterial lyase enzymes capable of acting on glycosaminoglycans, such as hyaluronan and chondroitin, in the extracellular matrix of host tissues, contributing to the invasive capacity of the pathogen. These are broad-specificity glycosaminoglycan lyases which recognize uronyl residues in polysaccharides and cleave their glycosidic bonds via a beta-elimination reaction to form a double bond between C-4 and C-5 of the non-reducing terminal uronyl residues of released products. Substrates include chondroitin, chondroitin 4-sulfate, chondroitin 6-sulfate, and hyaluronic acid. Family members include chondroitin AC lyase, chondroitin abc lyase, xanthan lyase, and hyalurate lyase.
pfam02278	Lyase_8	3.41e-48	708	963	3	252	Polysaccharide lyase family 8, super-sandwich domain. This family consists of a group of secreted bacterial lyase enzymes EC:4.2.2.1 capable of acting on hyaluronan and chondroitin in the extracellular matrix of host tissues, contributing to the invasive capacity of the pathogen.
cd01827	sialate_O-acetylesterase_like1	3.68e-48	1680	1870	1	188	sialate O-acetylesterase_like family of the SGNH hydrolases, a diverse family of lipases and esterases. The tertiary fold of the enzyme is substantially different from that of the alpha/beta hydrolase family and unique among all known hydrolases; its active site closely resembles the Ser-His-Asp(Glu) triad found in other serine hydrolases.
pfam13472	Lipase_GDSL_2	1.20e-28	1684	1858	1	174	GDSL-like Lipase/Acylhydrolase family. This family of presumed lipases and related enzymes are similar to pfam00657.
cd01834	SGNH_hydrolase_like_2	6.22e-23	1681	1868	3	191	SGNH_hydrolase subfamily. SGNH hydrolases are a diverse family of lipases and esterases. The tertiary fold of the enzyme is substantially different from that of the alpha/beta hydrolase family and unique among all known hydrolases; its active site closely resembles the Ser-His-Asp(Glu) triad found in other serine hydrolases.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QMW73769.1	9.50e-254	372	1484	30	1146
QPS12968.1	9.50e-254	372	1484	30	1146
QQY28197.1	3.58e-253	372	1484	30	1146
QQV04833.1	3.58e-253	372	1484	30	1146
QUN12787.1	3.34e-225	378	1483	36	1150

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
1CB8_A	3.35e-54	407	1033	28	634	CHONDROITINASEAC LYASE FROM FLAVOBACTERIUM HEPARINUM [Pedobacter heparinus]
1HM2_A	4.72e-54	407	1033	50	656	ACTIVESITE OF CHONDROITINASE AC LYASE REVEALED BY THE STRUCTURE OF ENZYME-OLIGOSACCHARIDE COMPLEXES AND MUTAGENESIS [Pedobacter heparinus],1HM3_A Active Site Of Chondroitinase Ac Lyase Revealed By The Structure Of Enzyme-Oligosaccharide Complexes And Mutagenesis [Pedobacter heparinus],1HMU_A ACTIVE SITE OF CHONDROITINASE AC LYASE REVEALED BY THE STRUCTURE OF ENZYME-OLIGOSACCHARIDE COMPLEXES AND MUTAGENESIS [Pedobacter heparinus],1HMW_A Active Site Of Chondroitinase Ac Lyase Revealed By The Structure Of Enzyme-oligosaccharide Complexes And Mutagenesis [Pedobacter heparinus]
2RV9_A	5.34e-20	1200	1332	7	136	Solutionstructure of chitosan-binding module 1 derived from chitosanase/glucanase from Paenibacillus sp. IK-5 [Paenibacillus fukuinensis]
4ZXE_A	5.49e-20	1200	1332	8	137	X-raycrystal structure of chitosan-binding module 1 derived from chitosanase/glucanase from Paenibacillus sp. IK-5. [Paenibacillus fukuinensis],4ZXE_B X-ray crystal structure of chitosan-binding module 1 derived from chitosanase/glucanase from Paenibacillus sp. IK-5. [Paenibacillus fukuinensis],4ZXE_C X-ray crystal structure of chitosan-binding module 1 derived from chitosanase/glucanase from Paenibacillus sp. IK-5. [Paenibacillus fukuinensis]
4ZY9_A	2.59e-19	1200	1332	8	137	X-raycrystal structure of selenomethionine-labelled V110M mutant of chitosan-binding module 1 derived from chitosanase/glucanase from Paenibacillus sp. IK-5 [Paenibacillus fukuinensis],4ZY9_B X-ray crystal structure of selenomethionine-labelled V110M mutant of chitosan-binding module 1 derived from chitosanase/glucanase from Paenibacillus sp. IK-5 [Paenibacillus fukuinensis]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
Q59288	2.59e-53	407	1033	50	656	Chondroitinase-AC OS=Pedobacter heparinus (strain ATCC 13125 / DSM 2366 / CIP 104194 / JCM 7457 / NBRC 12017 / NCIMB 9290 / NRRL B-14731 / HIM 762-3) OX=485917 GN=cslA PE=1 SV=1
D5EV35	1.70e-32	1680	1869	274	479	Acetylxylan esterase OS=Prevotella ruminicola (strain ATCC 19189 / JCM 8958 / 23) OX=264731 GN=axeA1 PE=1 SV=1
Q9AQS0	1.70e-17	414	1001	62	687	Xanthan lyase OS=Bacillus sp. (strain GL1) OX=84635 GN=xly PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.019685	0.966813	0.012149	0.000689	0.000330	0.000280

TMHMM  Annotations     

start	end
9	31