CAZyme Information: MGYG000004223_02118

Basic Information

• Species: UMGS1883 sp900763305
• Lineage: Bacteria; Firmicutes_A; Clostridia; UMGS1883; UMGS1883; UMGS1883; UMGS1883 sp900763305
• CAZyme ID: MGYG000004223_02118
• CAZy Family: GH2
• CAZyme Description: Beta-galactosidase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
931		104510.88	5.6536

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000004223	2575981	MAG	United Kingdom	Europe

• Gene Location: Start: 5603; End: 8398 Strand: +

Full Sequence      

MEYTIEKLNGSWEMKYSEGRYESEENPYGNIASEEDYAGTEPESVCADIIPGAVPGYWED
MTDKFREASFYSKLRFNPEYGVQSYPIAGNPPDTALPNITGSFFYRRSFIFKKAQGEKRI
YFGGVQNSVSVWLNGVFLGRHSGYSTPFEIPIPDENINDGENTIVLSVSNQRLCGCGGEP
VSGLTSRAANECTGGVTGGVEIRTYKSPLTDAAVLVSDDLKTVTVRTECLGAAALSWSVC
DGTRTIKTAAADGDFAFSADGLEYWSPENPKLYTLKIKCGDAVLERSFGVRRLAADGTRL
LLNGRKYFLRGACEHCCYPDTVHPNHDKAHYRRVIKKLKSLGFNHIRFHTYIPEEEYMQA
ADELGMLMHVECPNNTTVSEWREIVRFCRKHPSVVIYCCGNELLIDEPFIEYLRECAKIV
HEETDALFAPMSAMRGVEYFWCEPEQEKELKAEPFKHHPRRLKALGEFSDVYCTYPNGAH
SYFSTDCNPELIDKYSEVYQKPRLSHEICIDGTYTDLSVKDRYNGTRFGKTEMFSSVEKH
LADKGILKNAPLYFKNSCEWQRRVRKFCFENLRRSESVSGYDFLGPTDTHWHTFGYDVGM
MNEFYELKPGESVRNIRMYNSESVVLSDIGKRRNYAEGDTLSCNILMSYYGGRTLRGARL
TVRLFIGEDVHICKAFDAADIENGRVARLAAFSETLPYIGKAAEMRLYASVEADGFFAEN
EWELYLFPKIEPHSYDGITVSDGMTEEELAALLEKGKTVVLFGADPFKSNPTSFRIALAG
RTSGNLAAVVSEHPALENFPHGGFCGWQFAQMMEGGRAVCIENDRVQISPIIEVASTHKY
AVRQAAMFELGAINGKLIVCSFRFDENDPAANMLKELIAGYAAGESFKPEAYADEAALHA
LIHANVKQAASNTNLAFNANDKTAVRSNRKR

Enzyme Prediction     

No EC number prediction in MGYG000004223_02118.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH2	5	423	5.1e-62	0.46808510638297873

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
COG3250	LacZ	6.93e-28	5	403	12	407	Beta-galactosidase/beta-glucuronidase [Carbohydrate transport and metabolism].
PRK10150	PRK10150	2.82e-14	92	402	56	417	beta-D-glucuronidase; Provisional
pfam02837	Glyco_hydro_2_N	8.73e-12	5	170	1	135	Glycosyl hydrolases family 2, sugar binding domain. This family contains beta-galactosidase, beta-mannosidase and beta-glucuronidase activities and has a jelly-roll fold. The domain binds the sugar moiety during the sugar-hydrolysis reaction.
PRK10340	ebgA	1.06e-11	8	425	44	477	cryptic beta-D-galactosidase subunit alpha; Reviewed
pfam13364	BetaGal_dom4_5	1.07e-07	100	171	32	108	Beta-galactosidase jelly roll domain. This domain is found in beta galactosidase enzymes. It has a jelly roll fold.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
ASA21598.1	1.74e-184	5	889	7	893
ADB33533.1	2.94e-59	122	889	120	902
QDU88832.1	3.76e-48	72	889	93	938
AWI10104.1	3.26e-47	265	890	284	907
AYQ73318.1	1.95e-46	35	890	20	915

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
6D8K_A	1.64e-21	65	373	69	372	Bacteroidesmultiple species beta-glucuronidase [Bacteroides ovatus],6D8K_B Bacteroides multiple species beta-glucuronidase [Bacteroides ovatus],6D8K_C Bacteroides multiple species beta-glucuronidase [Bacteroides ovatus],6D8K_D Bacteroides multiple species beta-glucuronidase [Bacteroides ovatus]
6D89_A	1.53e-20	53	378	38	377	Bacteroidesuniformis beta-glucuronidase 1 with N-terminal loop deletion [Bacteroides uniformis],6D89_B Bacteroides uniformis beta-glucuronidase 1 with N-terminal loop deletion [Bacteroides uniformis],6D89_C Bacteroides uniformis beta-glucuronidase 1 with N-terminal loop deletion [Bacteroides uniformis],6D89_D Bacteroides uniformis beta-glucuronidase 1 with N-terminal loop deletion [Bacteroides uniformis]
7VQM_A	3.68e-20	8	436	16	443	ChainA, GH2 beta-galacturonate AqGalA [Aquimarina sp.],7VQM_B Chain B, GH2 beta-galacturonate AqGalA [Aquimarina sp.],7VQM_C Chain C, GH2 beta-galacturonate AqGalA [Aquimarina sp.],7VQM_D Chain D, GH2 beta-galacturonate AqGalA [Aquimarina sp.]
6D1N_A	8.45e-20	56	378	65	385	Apostructure of Bacteroides uniformis Beta-glucuronidase 1 [Bacteroides uniformis],6D1N_B Apo structure of Bacteroides uniformis Beta-glucuronidase 1 [Bacteroides uniformis],6D41_A Bacteriodes uniformis beta-glucuronidase 1 bound to D-glucaro-1,5-lactone [Bacteroides uniformis],6D41_B Bacteriodes uniformis beta-glucuronidase 1 bound to D-glucaro-1,5-lactone [Bacteroides uniformis],6D6W_A Bacteroides uniformis beta-glucuronidase 1 bound to glucuronate [Bacteroides uniformis],6D6W_B Bacteroides uniformis beta-glucuronidase 1 bound to glucuronate [Bacteroides uniformis],6D6W_C Bacteroides uniformis beta-glucuronidase 1 bound to glucuronate [Bacteroides uniformis],6D6W_D Bacteroides uniformis beta-glucuronidase 1 bound to glucuronate [Bacteroides uniformis],6D7F_A Bacteroides uniformis beta-glucuronidase 1 bound to thiophenyl-beta-D-glucuronide [Bacteroides uniformis],6D7F_B Bacteroides uniformis beta-glucuronidase 1 bound to thiophenyl-beta-D-glucuronide [Bacteroides uniformis],6D7F_C Bacteroides uniformis beta-glucuronidase 1 bound to thiophenyl-beta-D-glucuronide [Bacteroides uniformis],6D7F_D Bacteroides uniformis beta-glucuronidase 1 bound to thiophenyl-beta-D-glucuronide [Bacteroides uniformis],6D7F_E Bacteroides uniformis beta-glucuronidase 1 bound to thiophenyl-beta-D-glucuronide [Bacteroides uniformis],6D7F_F Bacteroides uniformis beta-glucuronidase 1 bound to thiophenyl-beta-D-glucuronide [Bacteroides uniformis]
6NCY_A	3.49e-19	79	425	83	439	Crystalstructure of hybrid beta-glucuronidase/beta-galacturonidase from Fusicatenibacter saccharivorans [Fusicatenibacter saccharivorans],6NCY_B Crystal structure of hybrid beta-glucuronidase/beta-galacturonidase from Fusicatenibacter saccharivorans [Fusicatenibacter saccharivorans],6NCZ_A Crystal structure of hybrid beta-glucuronidase/beta-galacturonidase from Fusicatenibacter saccharivorans bound to phenyl-thio-beta-D-glucuronide [Fusicatenibacter saccharivorans],6NCZ_B Crystal structure of hybrid beta-glucuronidase/beta-galacturonidase from Fusicatenibacter saccharivorans bound to phenyl-thio-beta-D-glucuronide [Fusicatenibacter saccharivorans],6NCZ_C Crystal structure of hybrid beta-glucuronidase/beta-galacturonidase from Fusicatenibacter saccharivorans bound to phenyl-thio-beta-D-glucuronide [Fusicatenibacter saccharivorans],6NCZ_D Crystal structure of hybrid beta-glucuronidase/beta-galacturonidase from Fusicatenibacter saccharivorans bound to phenyl-thio-beta-D-glucuronide [Fusicatenibacter saccharivorans],6NCZ_E Crystal structure of hybrid beta-glucuronidase/beta-galacturonidase from Fusicatenibacter saccharivorans bound to phenyl-thio-beta-D-glucuronide [Fusicatenibacter saccharivorans],6NCZ_F Crystal structure of hybrid beta-glucuronidase/beta-galacturonidase from Fusicatenibacter saccharivorans bound to phenyl-thio-beta-D-glucuronide [Fusicatenibacter saccharivorans]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
Q59140	6.19e-14	53	425	77	462	Beta-galactosidase OS=Arthrobacter sp. (strain B7) OX=86041 GN=lacZ PE=1 SV=1
Q56307	1.48e-11	8	402	42	441	Beta-galactosidase OS=Thermotoga maritima (strain ATCC 43589 / DSM 3109 / JCM 10099 / NBRC 100826 / MSB8) OX=243274 GN=lacZ PE=1 SV=2
P26257	7.31e-10	120	422	75	406	Beta-galactosidase OS=Thermoanaerobacterium thermosulfurigenes OX=33950 GN=lacZ PE=1 SV=1
P77989	2.23e-09	104	417	59	403	Beta-galactosidase OS=Thermoanaerobacter pseudethanolicus (strain ATCC 33223 / 39E) OX=340099 GN=lacZ PE=3 SV=2
Q9K9C6	9.98e-09	78	573	94	618	Beta-galactosidase OS=Alkalihalobacillus halodurans (strain ATCC BAA-125 / DSM 18197 / FERM 7344 / JCM 9153 / C-125) OX=272558 GN=lacZ PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000068	0.000000	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

There is no transmembrane helices in MGYG000004223_02118.