CAZyme Information: MGYG000004210_01422

Basic Information

• Lineage: Bacteria; Firmicutes_A; Clostridia; Lachnospirales; Lachnospiraceae; V9D3004
• CAZyme ID: MGYG000004210_01422
• CAZy Family: GH2
• CAZyme Description: Beta-galactosidase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
1363	MGYG000004210_6|CGC1	151673.83	5.3026

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000004210	2253787	MAG	United Kingdom	Europe

• Gene Location: Start: 53440; End: 57531 Strand: +

Full Sequence      

MQMFMHRYFKRFVSLLLIIIMSGTVIPVMEQNTVNAAENVASFTRQTINFNREWKFVRDN
VEGAEQENYNDSSWYNVGLPHDFSIPYWQEEKHYTGYGWYRKDFDVKKEWIGKHLTLDFA
GVFHTAEVYVNGQFAGEHEGGYTGFEIDISGFVREGSNTVAVCVNNIWKDYLAPRAGEHM
FTGGIYRDVNLVITSPVHVSWYGTFVQTPDINAERSNVRVLAEIANNSGASQTVSVTHTV
YDADKKAVASIDSGNVQIASNTTYEFDNTSGYIQNINLWSPENPYLYSVHTDVHVNGQSV
DQYDTTFGFRWFEFDKDEGFFLNGEHYWIDGANAHQDHAGWANAVTAEALKRDVSMIKES
GLNFIRGSHYPHSPAYADACDELGVLFWSEAAFWCTAAYGEGSQNGTSEDYKADGYPTTG
NKATEEAFEQSCIDCLTEMIRINRNHPSIIVWSMGNEPFFGSNAEKKKKLISKMAGVAKE
LDNTRYTAIGGTQRGEFDHLPNVDIAGYNGDGASMPEYQDPQVANLVAEYSSHTGNRPDK
YTAYYGSVQVDDNNEAIRYPWRSGIALWCAFHHGSIMARSYGDMGYIDYYRLPLNTWYYY
RYNRTGTEPEFSQEGTPARLSLSASDTTIKNDGTSDTHIIVTVTDDEGNWLNSEPEVTLE
VIEGPGLFPTGKTMTFKPGDSMRDGKAAIEFRSYYAGVTTIEAYSASNPELEPATIQITT
VGEGSVDEPDITTMYGAFMSNGGYVPNSIEEPSAYKYHSFNGCPMNASSGLETVQNVQDG
DDFTSWTASASGSNQWLNMEIEHGGINLYKARLTFNGKVYPYKIQYKAVNISDEEWITIK
EYDSNTINLRPYEESFGGTYMRYIRIEFTDVPQNEYANIGEIKLYGIRADQDGYEVGIRN
LSDKSEDIECETNSELVYDLENNAKNYARFQSDIIFDSQTELKSPVKVSVYCDEELMFEK
TISNPDDVTSIDISVNKVKALKLCAASDTEGIAFKWSNARLIGALRDISVSQSRVNTQIC
SGMDPLIPGEKLTMQARFENKAEEKAELGLAQVLYSADGTALAADITAVSVPGKGNKVIN
LNMDIPADIEKLSYVKIMAWNKDTLMPVTEDAYIFNETGAVGAAAAGLRNDAGLLSAIKD
AMNIIYSGDADRYTSDSRKGYTEALVKGSAVLNDASLPQSDVDSAENAIRNAIAGLKLKT
TVSDDTVTIPADQAAQPYTQGINDAIKKGGIYKAGKYRYKVLKVSDKTVNVSLYRPVSKK
MKTAVIPAAVKIKGRSCKVTAVSAKAFMNNKKLKKVVVGKNVTKIGKKAFFKTPGLSLVT
VKSTRLKKVYKNTFRKSSKKLIVRAPEKKLKAYKKLMKNVKVR

Enzyme Prediction     

No EC number prediction in MGYG000004210_01422.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH2	30	607	2.7e-112	0.6382978723404256

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
COG3250	LacZ	1.90e-66	36	591	1	525	Beta-galactosidase/beta-glucuronidase [Carbohydrate transport and metabolism].
PRK10150	PRK10150	2.33e-56	44	485	9	443	beta-D-glucuronidase; Provisional
PRK10340	ebgA	2.51e-48	35	485	29	470	cryptic beta-D-galactosidase subunit alpha; Reviewed
PRK09525	lacZ	1.89e-28	98	463	122	468	beta-galactosidase.
pfam02836	Glyco_hydro_2_C	4.91e-26	316	544	4	224	Glycosyl hydrolases family 2, TIM barrel domain. This family contains beta-galactosidase, beta-mannosidase and beta-glucuronidase activities.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
AUI46287.1	4.59e-225	41	886	29	860
AKA51668.1	6.46e-225	41	886	29	860
QCQ37034.1	2.54e-224	41	886	29	860
BAD48632.1	5.03e-224	41	886	29	860
ANQ60352.1	7.09e-224	41	886	29	860

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
7CWD_A	1.72e-75	48	722	4	691	ChainA, beta-glalactosidase [Niallia circulans],7CWI_A Chain A, beta-galactosidase [Niallia circulans]
6D8G_A	1.09e-73	45	537	33	534	D341AD367A calcium binding mutant of Bacteroides uniformis beta-glucuronidase 2 [Bacteroides uniformis str. 3978 T3 ii],6D8G_B D341A D367A calcium binding mutant of Bacteroides uniformis beta-glucuronidase 2 [Bacteroides uniformis str. 3978 T3 ii]
5UJ6_A	7.76e-73	45	537	25	526	CrystalStructure of Bacteroides Uniformis beta-glucuronidase [Bacteroides uniformis str. 3978 T3 ii],5UJ6_B Crystal Structure of Bacteroides Uniformis beta-glucuronidase [Bacteroides uniformis str. 3978 T3 ii],6NZG_A Bacteroides uniformis beta-glucuronidase 2 covalently bound to cyclophellitol-6-carboxylate aziridine [Bacteroides uniformis],6NZG_B Bacteroides uniformis beta-glucuronidase 2 covalently bound to cyclophellitol-6-carboxylate aziridine [Bacteroides uniformis]
6D50_A	8.74e-73	45	537	33	534	Bacteroidesuniforms beta-glucuronidase 2 bound to D-glucaro-1,5-lactone [Bacteroides uniformis str. 3978 T3 ii],6D50_B Bacteroides uniforms beta-glucuronidase 2 bound to D-glucaro-1,5-lactone [Bacteroides uniformis str. 3978 T3 ii]
4YPJ_A	5.79e-71	48	601	10	596	ChainA, Beta galactosidase [Niallia circulans],4YPJ_B Chain B, Beta galactosidase [Niallia circulans]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
T2KPJ7	1.19e-61	42	557	50	545	Putative beta-glucuronidase OS=Formosa agariphila (strain DSM 15362 / KCTC 12365 / LMG 23005 / KMM 3901 / M-2Alg 35-1) OX=1347342 GN=BN863_21970 PE=2 SV=1
A7LXS9	8.25e-54	45	530	42	535	Beta-galactosidase BoGH2A OS=Bacteroides ovatus (strain ATCC 8483 / DSM 1896 / JCM 5824 / BCRC 10623 / CCUG 4943 / NCTC 11153) OX=411476 GN=BACOVA_02645 PE=1 SV=1
P77989	1.35e-53	45	601	3	533	Beta-galactosidase OS=Thermoanaerobacter pseudethanolicus (strain ATCC 33223 / 39E) OX=340099 GN=lacZ PE=3 SV=2
T2KM09	1.16e-50	49	534	50	496	Putative beta-glucuronidase OS=Formosa agariphila (strain DSM 15362 / KCTC 12365 / LMG 23005 / KMM 3901 / M-2Alg 35-1) OX=1347342 GN=BN863_22050 PE=2 SV=2
P26257	1.17e-47	45	601	2	542	Beta-galactosidase OS=Thermoanaerobacterium thermosulfurigenes OX=33950 GN=lacZ PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.000289	0.999035	0.000177	0.000192	0.000149	0.000132

TMHMM  Annotations     

start	end
12	29