CAZyme Information: MGYG000004184_01592

Basic Information

• Lineage: Bacteria; Firmicutes_A; Clostridia; Oscillospirales; Ruminococcaceae; QANG01
• CAZyme ID: MGYG000004184_01592
• CAZy Family: GH127
• CAZyme Description: Non-reducing end beta-L-arabinofuranosidase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
605		69299.29	4.9015

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000004184	2370952	MAG	United Kingdom	Europe

• Gene Location: Start: 3960; End: 5777 Strand: +

Full Sequence      

MEKIDFSNTKINNGFWKQKQDMVNNSTLYSVYDRFVDTHRFDALSCRWKAGDPDMPHIFW
DSDVAKWMEGAAYILQEKRDEKLEAIIDDAIDNIVKNSDENGYFNSHYLVTEQDKRFSDR
SCHELYCAGHLIEAAIAYNNATGKGKFLKAMCKFADYIEKVFKIEKSAAFTTPGHPELEL
ALVKLYDATGERRYLELSKFFIDEHGTKKEEHDSNYDYNNLLYNQDEMPLRKRSTAEGHC
VRALYLLCGAADIADRYNDCELKDACKRVFDDIANNKVYITGGVGSSHLGEAFTVPYHLP
NRTAYTETCAAISLAMFCNRMLQIDTDSKYADMAERAIYNGFLSGVSMDGKSFFYENPLE
IDPRFNHVNPSVINKERFPITQRLEVFDCSCCPPNIVRFIPSIADFLYTYNDETVYVHQY
IDSVTEYGDIKISQNTGYPANGEIKIQCTAPQKKIALRIPSWCQNFSLNVDYTLKNGYAY
IEMNDSIEIELKLDMPVVFIKANKRVHENAGRIAVMRGPVVYCGEAVDNGNDLRCIEIDT
NAPAKIEEGEFILPSVTLQAFRQKDDDKLYSPVGDDYEPCTLQLIPYYAFANRGESEMLV
WFLKK

Enzyme Prediction     

No EC number prediction in MGYG000004184_01592.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH127	10	523	4.4e-177	0.9961832061068703

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
COG3533	COG3533	4.79e-166	13	601	20	584	Uncharacterized conserved protein, DUF1680 family [Function unknown].
pfam07944	Glyco_hydro_127	4.08e-138	8	523	1	503	Beta-L-arabinofuranosidase, GH127. One member of this family, from Bidobacterium longicum, UniProtKB:E8MGH8, has been characterized as an unusual beta-L-arabinofuranosidase enzyme, EC:3.2.1.185. It rleases l-arabinose from the l-arabinofuranose (Araf)-beta1,2-Araf disaccharide and also transglycosylates 1-alkanols with retention of the anomeric configuration. Terminal beta-l-arabinofuranosyl residues have been found in arabinogalactan proteins from a mumber of different plantt species. beta-l-Arabinofuranosyl linkages with 1-4 arabinofuranosides are also found in the sugar chains of extensin and solanaceous lectins, hydroxyproline (Hyp)2-rich glycoproteins that are widely observed in plant cell wall fractions. The critical residue for catalytic activity is Glu-338, in a ET/SCAS sequence context.
COG2942	YihS	2.04e-05	154	338	151	332	Mannose or cellobiose epimerase, N-acyl-D-glucosamine 2-epimerase family [Carbohydrate transport and metabolism].

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
AUS96918.1	1.44e-193	4	605	12	628
QHQ63226.1	4.07e-187	4	603	9	623
AZN43118.1	1.11e-169	4	605	13	657
AGB41651.1	3.12e-153	1	601	3	642
AZO96465.1	4.75e-151	1	604	8	651

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
4QJY_A	2.98e-149	1	605	9	648	Crystalstructure of native Ara127N, a GH127 beta-L-arabinofuranosidase from Geobacillus Stearothermophilus T6 [Geobacillus stearothermophilus],4QJY_B Crystal structure of native Ara127N, a GH127 beta-L-arabinofuranosidase from Geobacillus Stearothermophilus T6 [Geobacillus stearothermophilus]
4QK0_A	9.40e-145	1	605	9	648	Crystalstructure of Ara127N-Se, a GH127 beta-L-arabinofuranosidase from Geobacillus Stearothermophilus T6 [Geobacillus stearothermophilus],4QK0_B Crystal structure of Ara127N-Se, a GH127 beta-L-arabinofuranosidase from Geobacillus Stearothermophilus T6 [Geobacillus stearothermophilus],4QK0_C Crystal structure of Ara127N-Se, a GH127 beta-L-arabinofuranosidase from Geobacillus Stearothermophilus T6 [Geobacillus stearothermophilus],4QK0_D Crystal structure of Ara127N-Se, a GH127 beta-L-arabinofuranosidase from Geobacillus Stearothermophilus T6 [Geobacillus stearothermophilus]
3WRE_A	5.02e-121	8	601	2	655	Thecrystal structure of native HypBA1 from Bifidobacterium longum JCM 1217 [Bifidobacterium longum subsp. longum JCM 1217],3WRG_A The complex structure of HypBA1 with L-arabinose [Bifidobacterium longum subsp. longum JCM 1217]
3WKW_A	6.80e-121	8	601	2	655	Crystalstructure of GH127 beta-L-arabinofuranosidase HypBA1 from Bifidobacterium longum ligand free form [Bifidobacterium longum subsp. longum JCM 1217],3WKX_A Crystal structure of GH127 beta-L-arabinofuranosidase HypBA1 from Bifidobacterium longum arabinose complex form [Bifidobacterium longum subsp. longum JCM 1217],7BZL_A Chain A, Non-reducing end beta-L-arabinofuranosidase [Bifidobacterium longum subsp. longum JCM 1217],7DIF_A Chain A, Non-reducing end beta-L-arabinofuranosidase [Bifidobacterium longum subsp. longum JCM 1217],7EXV_A Chain A, Non-reducing end beta-L-arabinofuranosidase [Bifidobacterium longum subsp. longum JCM 1217],7EXW_A Chain A, Non-reducing end beta-L-arabinofuranosidase [Bifidobacterium longum subsp. longum JCM 1217]
7EXU_A	1.90e-120	8	601	2	655	ChainA, Non-reducing end beta-L-arabinofuranosidase [Bifidobacterium longum subsp. longum JCM 1217]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
E8MGH8	2.75e-120	8	601	2	655	Non-reducing end beta-L-arabinofuranosidase OS=Bifidobacterium longum subsp. longum (strain ATCC 15707 / DSM 20219 / JCM 1217 / NCTC 11818 / E194b) OX=565042 GN=hypBA1 PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000052	0.000000	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

There is no transmembrane helices in MGYG000004184_01592.