CAZyme Information: MGYG000004157_01005

Basic Information

• Species: HGM11514 sp900757255
• Lineage: Bacteria; Firmicutes_A; Clostridia; HGM11514; HGM11514; HGM11514; HGM11514 sp900757255
• CAZyme ID: MGYG000004157_01005
• CAZy Family: GH16
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
2012		217588.1	4.379

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000004157	2480742	MAG	United Kingdom	Europe

• Gene Location: Start: 1334; End: 7372 Strand: +

Full Sequence      

MARNTKKLWAILLVVVMLTGMMPEFIVNAAAVTELRMDPRVNAGSITPWSKVTPAPGVTY
ESTGTGWSANSGLTNSVGEQKTLYNANEGSSVKFEPNASGDTLAPGWYDVSFWNPKTAGT
RNFNATVFANGVTQLDDVEQPLGTASSWSKVGRFYFAGTQGEYFQLAVSGSGTGMARVGD
VKFAWSADQGGELTSPPPSSEPSPAEPSEAPSSVPFAGWSEDFTGQTAAGSGFASGTNGG
PVEFDIAERSAGNNALKAWMADDKTMTQLNGPSVKDKNNGNKVYGENMVITAKFKIEKQP
DKVGTTTDTQFIIRSADNKVISHFSFQSISEDKLAVGYRYGAGSTAVKPVDTKMELNKDE
WYQLLMGLNSLTGTYDFYFLDESGEIVLRTDGRPAAEENVAVRYDTAQTGGIADVAYFQV
NNFLPGTVMWFDDFKIANDPDYHFPDLSQLPSPEPSDKPSAEPSSLPSAEPFAGWREDFT
GQTATGTGFTSPGDVEIAIAERAPGNSALRASLEKGRIKTNMDSPSLRAKNNGNKVFGEN
IVISTDFMIEKQTVSSQSKDSQFIIRSADNKVIAHFSLQSLSATEIGVGYRYGADSTAVG
PTSSKIVLEKGVWYQLLIGVNSRLGTYDFYFLDDSGNVVFRTDNTPAAEEGLPVRYDVST
TGGIADVDYFQVNNFLDDSVLWFDDFYIANDPNYIFPDLETLPTPSPRPTFPPAGAPVFT
VLDDLDKFEQIVPDTVRDSDMGGSATGGSLHLYAHSDQVTGVFHLVDVPTGIYEVYYKVP
LVHSSNTDKMTLDIVDSVGKTDSGIYDTKSIEDLPGHTDNISTQGIKTQSWIKLDGQYTF
YPQIPGTLTAGKVKGATGAVRYEAVTLIKVGDTAEIPLAYEVQISGKNKPGAELTGTYKF
ADNNGNTESGSVYKWYTKAAENAPWSVAASGTTTAAEGAAYTIKETDKFIKFEITPKSNA
SETGLQTGLAAGCELTVYDEQPPAANNVVITGEPQQGAKLSASYEFSDVNGDLEGDTAYK
WLLGDGPETPLGNWKVLEEGTAKAGTPIEFTVPVTVPDGKYIRIAITPKNDDPALEKGET
VYSNLIGPFQNTAIKPKAINLTFGGQAVNANGLNGLAIDGEAKADYIYTYALGLPENKIQ
TKYQWYSADLQTGPFTPIAGATKETYIPTQADSGKYIRFSVTPVSEDGVEGETVYSDSYI
VKWKLSFYDEFEYDAANADMAPFTDKWTSDRSQRTLGEPIPIEQMRIPENTSVSEGSMKI
TTRKEHLDKYKEDHTWTTGNVMTKETWGPYGYYESRLKFAAATGLNQAFWTITRGDTTIE
AGFLELDFLEGHYPYELTNSIHRYDIENGSQKRFAKSIKSYPFGRPGTETLADNFNILGG
YLKPNDSLYTWNAEENQDTYQIYFNNNLLRSTESVQYVPSPGRIMFSIAVFPGWCGILND
QADGSVFEMDYVRFYEEIGVTQNGTATYDIANTELQSAVAMANQILQNAVIGNAAGNYTQ
QAKAELQNAVAKANAVTDNAKKAAAANELNQAVKEFQKKQIGDRTALQAKIAQAEALLKA
TSSGDAYGQCPANYIRVLNGQMDVAKQLVAGTPTQDQLDSSANSLQIAIDNVIAKKAEGK
TVTSDNQMIDIASIIAVGPGELTFEPSASKAQLILPDEFGYDMTLHFKNNKGKSVSITIP
EGTRVLSGNSAVFEWLNPQISGKSVSGAFSTGDFATDKLIRIEVTRGTSENLNRLNGSAL
SEFDAVIETDSFDAANAGYSGSGAYTAKYDGSGISVLYSSQLGSFAISSDSSEQPDDNDN
NNNNNNNNNQSQTNPGGAPGGMVIPGGTNSNKPSVNFKDISGHWAEKEIRELAQAGIVNG
RSETQYAPEENVSRAEFAALIRRALKINPQLYKGGFSDVSEGDWYANEIQAIADAGIMSG
DTNGAFRPNDTITREEMAKVIVNAYKVMTGADNIIGADVSFNDSQSISGWAQDYVKQAVS
LNLMNGMGDGIFVPQGYATRAQSAVVIYRIWN

Enzyme Prediction     

No EC number prediction in MGYG000004157_01005.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH16	1209	1455	2e-17	0.9788359788359788

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
NF033190	inl_like_NEAT_1	4.42e-26	1821	2008	561	749	NEAT domain-containing leucine-rich repeat protein. Members of this family have an N-terminal NEAT (near transporter) domain often associated with iron transport, followed by a leucine-rich repeat region with significant sequence similarity to the internalins of Listeria monocytogenes. However, since Bacillus cereus (from which this protein was described, in PMID:16978259) is not considered an intracellular pathogen, and the function may be iron transport rather than internalization, applying the name "internalin" to this family probably would be misleading.
cd00413	Glyco_hydrolase_16	5.54e-14	1209	1455	1	210	glycosyl hydrolase family 16. The O-Glycosyl hydrolases are a widespread group of enzymes that hydrolyse the glycosidic bond between two or more carbohydrates, or between a carbohydrate and a non-carbohydrate moiety. A glycosyl hydrolase classification system based on sequence similarity has led to the definition of more than 95 different families inlcuding glycosyl hydrolase family 16. Family 16 includes lichenase, xyloglucan endotransglycosylase (XET), beta-agarase, kappa-carrageenase, endo-beta-1,3-glucanase, endo-beta-1,3-1,4-glucanase, and endo-beta-galactosidase, all of which have a conserved jelly roll fold with a deep active site channel harboring the catalytic residues.
NF033190	inl_like_NEAT_1	1.55e-12	1837	1946	643	753	NEAT domain-containing leucine-rich repeat protein. Members of this family have an N-terminal NEAT (near transporter) domain often associated with iron transport, followed by a leucine-rich repeat region with significant sequence similarity to the internalins of Listeria monocytogenes. However, since Bacillus cereus (from which this protein was described, in PMID:16978259) is not considered an intracellular pathogen, and the function may be iron transport rather than internalization, applying the name "internalin" to this family probably would be misleading.
pfam00395	SLH	1.89e-12	1896	1937	1	42	S-layer homology domain.
cd02178	GH16_beta_agarase	5.97e-12	1202	1455	18	257	Beta-agarase, member of glycosyl hydrolase family 16. Beta-agarase is a glycosyl hydrolase family 16 (GH16) member that hydrolyzes the internal beta-1,4-linkage of agarose, a hydrophilic polysaccharide found in the cell wall of Rhodophyceaea, marine red algae. Agarose is a linear chain of galactose units linked by alternating L-alpha-1,3- and D-beta-1,4-linkages that are additionally modified by a 3,6-anhydro-bridge. Agarose forms thermo-reversible gels that are widely used in the food industry or as a laboratory medium. While beta-agarases are also found in two other families derived from the sequence-based classification of glycosyl hydrolases (GH50, and GH86) the GH16 members are most abundant. This domain adopts a curved beta-sandwich conformation, with a tunnel-shaped active site cavity, referred to as a jellyroll fold.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QUI25357.1	7.56e-132	742	1463	540	1288
QHI71623.1	9.42e-73	1091	1470	44	420
AEF17757.1	1.34e-39	1820	2010	1035	1225
AAA23227.1	1.38e-39	1832	2010	903	1080
ADL68522.1	3.73e-38	1837	2010	1103	1275

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
6BT4_A	6.21e-16	1837	2009	26	194	Crystalstructure of the SLH domain of Sap from Bacillus anthracis in complex with a pyruvylated SCWP unit [Bacillus anthracis]
3PYW_A	6.36e-16	1837	2009	5	173	Thestructure of the SLH domain from B. anthracis surface array protein at 1.8A [Bacillus anthracis]
4AQ1_A	2.27e-12	1835	2012	2	169	Structureof the SbsB S-layer protein of Geobacillus stearothermophilus PV72p2 in complex with nanobody KB6 [Geobacillus stearothermophilus]
4AQ1_C	2.27e-12	1835	2012	2	169	Structureof the SbsB S-layer protein of Geobacillus stearothermophilus PV72p2 in complex with nanobody KB6 [Geobacillus stearothermophilus]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
P38535	2.76e-40	1832	2010	903	1080	Exoglucanase XynX OS=Acetivibrio thermocellus OX=1515 GN=xynX PE=3 SV=1
P38536	5.39e-38	1837	2010	1682	1854	Amylopullulanase OS=Thermoanaerobacterium thermosulfurigenes OX=33950 GN=amyB PE=3 SV=2
P19424	3.83e-26	1829	2007	30	210	Endoglucanase OS=Bacillus sp. (strain KSM-635) OX=1415 PE=1 SV=1
P36917	1.87e-23	1832	1939	1051	1157	Endo-1,4-beta-xylanase A OS=Thermoanaerobacterium saccharolyticum OX=28896 GN=xynA PE=1 SV=1
C6CRV0	1.86e-20	1844	2012	1291	1460	Endo-1,4-beta-xylanase A OS=Paenibacillus sp. (strain JDR-2) OX=324057 GN=xynA1 PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.003458	0.995348	0.000605	0.000232	0.000184	0.000181

TMHMM  Annotations     

start	end
9	31