CAZyme Information: MGYG000003986_01125

Basic Information

• Species: CAG-485 sp900761855
• Lineage: Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Muribaculaceae; CAG-485; CAG-485 sp900761855
• CAZyme ID: MGYG000003986_01125
• CAZy Family: GT4
• CAZyme Description: Alpha-maltose-1-phosphate synthase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
370		41554.99	9.4264

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000003986	2429324	MAG	United Kingdom	Europe

• Gene Location: Start: 32686; End: 33798 Strand: +

Full Sequence      

MRIAVTGTRGIPRIQGGVETHCEELFPRIVSLGADVTLYRRASYVEADNRIDSYKGVALA
DIPTPRRKSFEAIIHTFRAVLTAKRSGADIIHIHAIGPALLVPFARMLGLKVVMTNHGPD
YDREKWGRLAKMMLRLGERFGTTSADAVIVISRHIQNILAEKYGRTDTDLIFNGVPEPIL
DSDTDYLTALGLEPRKYVVALGRFVPEKNFHLLAEAFSRLKPARYRLVIAGDADMPDRYS
EELKENARRAGVVLTGFIKGKKLHQLMTHARLFVLPSTHEGLPISLLEAMSYGLDVLVSD
IAANKLPELERSDYFRTSDIESLIAALSRKLAEDLPPATDPTSRIRTYDMSAYDWDNIAR
RTLEVYRRLI

Enzyme Prediction     

No EC number prediction in MGYG000003986_01125.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GT4	194	327	9.7e-25	0.85625

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
cd03801	GT4_PimA-like	3.36e-49	2	367	1	366	phosphatidyl-myo-inositol mannosyltransferase. This family is most closely related to the GT4 family of glycosyltransferases and named after PimA in Propionibacterium freudenreichii, which is involved in the biosynthesis of phosphatidyl-myo-inositol mannosides (PIM) which are early precursors in the biosynthesis of lipomannans (LM) and lipoarabinomannans (LAM), and catalyzes the addition of a mannosyl residue from GDP-D-mannose (GDP-Man) to the position 2 of the carrier lipid phosphatidyl-myo-inositol (PI) to generate a phosphatidyl-myo-inositol bearing an alpha-1,2-linked mannose residue (PIM1). Glycosyltransferases catalyze the transfer of sugar moieties from activated donor molecules to specific acceptor molecules, forming glycosidic bonds. The acceptor molecule can be a lipid, a protein, a heterocyclic compound, or another carbohydrate residue. This group of glycosyltransferases is most closely related to the previously defined glycosyltransferase family 1 (GT1). The members of this family may transfer UDP, ADP, GDP, or CMP linked sugars. The diverse enzymatic activities among members of this family reflect a wide range of biological functions. The protein structure available for this family has the GTB topology, one of the two protein topologies observed for nucleotide-sugar-dependent glycosyltransferases. GTB proteins have distinct N- and C- terminal domains each containing a typical Rossmann fold. The two domains have high structural homology despite minimal sequence homology. The large cleft that separates the two domains includes the catalytic center and permits a high degree of flexibility. The members of this family are found mainly in certain bacteria and archaea.
COG0438	RfaB	2.37e-40	1	370	1	378	Glycosyltransferase involved in cell wall bisynthesis [Cell wall/membrane/envelope biogenesis].
cd03809	GT4_MtfB-like	5.88e-40	2	364	1	362	glycosyltransferases MtfB, WbpX, and similar proteins. This family is most closely related to the GT4 family of glycosyltransferases. MtfB (mannosyltransferase B) in E. coli has been shown to direct the growth of the O9-specific polysaccharide chain. It transfers two mannoses into the position 3 of the previously synthesized polysaccharide.
cd03798	GT4_WlbH-like	8.20e-32	88	369	96	376	Bordetella parapertussis WlbH and similar proteins. This family is most closely related to the GT4 family of glycosyltransferases. Staphylococcus aureus CapJ may be involved in capsule polysaccharide biosynthesis. WlbH in Bordetella parapertussis has been shown to be required for the biosynthesis of a trisaccharide that, when attached to the B. pertussis lipopolysaccharide (LPS) core (band B), generates band A LPS.
cd03811	GT4_GT28_WabH-like	2.59e-29	16	301	12	294	family 4 and family 28 glycosyltransferases similar to Klebsiella WabH. This family is most closely related to the GT1 family of glycosyltransferases. WabH in Klebsiella pneumoniae has been shown to transfer a GlcNAc residue from UDP-GlcNAc onto the acceptor GalUA residue in the cellular outer core.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
AHF12040.1	2.63e-147	1	369	1	361
QUT23979.1	1.32e-144	1	369	1	359
QNL37901.1	1.88e-144	1	370	1	360
BCI64460.1	1.54e-141	1	370	1	362
BAN68889.1	2.19e-140	1	366	1	360

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
6TVP_A	1.47e-16	1	369	15	399	Structureof Mycobacterium smegmatis alpha-maltose-1-phosphate synthase GlgM [Mycolicibacterium smegmatis MC2 155],6TVP_B Structure of Mycobacterium smegmatis alpha-maltose-1-phosphate synthase GlgM [Mycolicibacterium smegmatis MC2 155]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
A0R2E2	7.39e-16	1	369	1	385	Alpha-maltose-1-phosphate synthase OS=Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155) OX=246196 GN=glgM PE=1 SV=1
Q8S4F6	3.04e-10	93	355	193	465	Sulfoquinovosyl transferase SQD2 OS=Arabidopsis thaliana OX=3702 GN=SQD2 PE=1 SV=1
P71055	7.27e-09	189	306	197	315	Putative glycosyltransferase EpsF OS=Bacillus subtilis (strain 168) OX=224308 GN=epsF PE=2 SV=1
Q44571	7.47e-09	87	370	95	378	GDP-mannose:cellobiosyl-diphosphopolyprenol alpha-mannosyltransferase OS=Komagataeibacter xylinus OX=28448 GN=aceC PE=1 SV=1
B2SUK8	4.04e-08	79	366	79	366	GDP-mannose:cellobiosyl-diphosphopolyprenol alpha-mannosyltransferase OS=Xanthomonas oryzae pv. oryzae (strain PXO99A) OX=360094 GN=gumH PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000055	0.000003	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

There is no transmembrane helices in MGYG000003986_01125.