CAZyme Information: MGYG000003984_00613

Basic Information

• Species: Mediterraneibacter sp900752395
• Lineage: Bacteria; Firmicutes_A; Clostridia; Lachnospirales; Lachnospiraceae; Mediterraneibacter; Mediterraneibacter sp900752395
• CAZyme ID: MGYG000003984_00613
• CAZy Family: GH31
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
2239	MGYG000003984_6|CGC1	245676.54	4.4131

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000003984	2095190	MAG	United Kingdom	Europe

• Gene Location: Start: 19415; End: 26134 Strand: -

Full Sequence      

MKWTKKMKGAGKRAAVTVTALGMVLTAFPPIPAKAAGEFAGKLTSVESVKKGNADNIVIV
TFNGGVEAKLTFLEDGIFRYNVDPSGEFSEYAKPRQSFHKGRIQQYPDSSSEYSKPEAKI
KDADGKFMITSGTVSVEFDKETAKMKILSNDKVVMEETEALSISKSATVQTLKKNDGENF
YGGGCQNGRFVHTGESINIANESNWTNGGVSSPNPFYITTEGYGVLRNTFADGKYDFGKT
TADAVTATHNEGELDAYYFVSDGKNVTEKTQELLREYYHVSGNPTLLPEYAFYEGHLNAY
NRDAWSNTPTNGAKAWTIKGNDSSDSEGKTTYEIGRSADFVPVDGQQLESLNGKAPTVAT
EKYPNVTTPEEFSARAVLDDYVEYDMPLGYFLPNDGYGSGYGQNGHHMTGGVNSDGTSSK
ERLDAVAANIQNLAEFTEYAQGKGIQTGLWTQSYLVPDSNSGTLWHRLRDFENEVKVGGI
STLKTDVAWVSQGYSMQLDGVKSAYDIATTKGKIRPNIISLDGWAGSQRYCGIWTGDQYG
GEWEYIRFHIPTYLGMALSGIPNLGSDMDGIFGGSPIIATRDYQWKSFSPLMLNMDGWGT
YAKMPYVFGDPYTGLNRMYMKIKAQMLPYIYTGAASGSNIDTGNGDTGIPLVRAMFLEYP
NDSYANSKAVQYQYMFGSDLLVAPIYQNTADTENTGNDIRNNIYLPGEEDVWIDYFTGEQ
YQGGQVLNNFDAPLWKLPLFVKNGAIIPMWEENNSPEEIDKANRIVEFWPSGKNSYTMFE
DDGKFVENNLTEDKEYGQITDVSYGNHVSTKFTSEVKDGTATLTAEKSTGDYTGYKKDKN
TTFVVNVSKKPTKVTAKNGSNTLEVEEVQNKADFDTKKVEAGKAVYFYDESPVIETYASE
KETAVAELVKDVKRTPKLYVKFAQADAKAVDQTLVIEGFANDGKLPKDQLNENLAVPTLT
ENVDAKTPTSITLGWNKVDTATSYELMIDDVVFAVGDANAYTHVDLTYHSEHTYQVRSRN
ADGYSKWSEKQTFQSAEDPWKDTPTPQTITWSGEIYGNHKADLAFDKVFQQGDSGFHSNG
NSIGQTLTVDYGKAYEIDKIEYYPRTDASNGTVKKMKLETSLDGVHWTEAKEYTWEINAE
TKVMGDLNCGARYIRFTPLESVGNFFAASEIKVYKKENTGAFAVGSTNKADTVSDGDYTN
MKNYLGTCEKDSLFVDQIKSRYGDINMNNIYDVYDYAFTMFQLDGGTKKTDSVSGEAMLL
ASSADVKKGETFTIDVYAQNVAGLNAFGQVINYDPKKVEFVSCEGSPLLAQMENLTVNKV
YDDGAYVNLAYANRGDKNLYAGSDVLATITMKAKDDISTENKNVIDLSKVTLIGPNYSTI
ESEVNTDVEIPDVPATEKKFAQNDFNITMTNEKLTEDSADDPNVNKLIQQNNYNGLFDGT
FGRDFEFKWDIPANQVDGKLPDYIILPTTMHLALKNPEALNKVAVSNANKGNGYLTSVSA
KLIYTDDTASDEIKFEAEQEKYTFEFSGDKAVKEVQITFLDAKSTSDVKNMLTLAELELS
NLSNTPVTGITADPNNVKEMYVGTLADINATVQPDNATNKFFTAESSNEDVVKILTLADE
NGYPVYKARAMKEGKSTITLAAAGNKDVKATYEITVKAGVDISGLNEALAKARNHQSSAY
TEESYGQLTAAVNAATELLKGEYTKNQVLEAQMAIYDAIDGLVFRPLDETKLLDAKAEGF
TVTATSECDPDKLEDGLATNVLDGKEDNYWHTEYNKDVLPQSLNFDLGGLYNLTDITFLA
RQGATNGDILKAQIFVGSAKDDMKSVGTYEFDENGNVLVNRDQYQQIAFDAKDVRYVEFK
VLEAGAQDKFASMAEIRFYGEKTTAALKALYDSYVAENLNKADYTADSWAVYEAKMDEAK
ALIEAKDTTNAAAGEALTALQTAHDALAKDETPTPGEADKTALKALCDQADALKSDDYTA
DSWAEFLLALREAKSVLGDETVTQDVVNQAVTDLQVAIEALVKNDPEPGDVDKSGLQNLY
NQYKDTKPDGYTESSWLDFAAALDKAEKVLKDEKATVGDVQAAVAELEMAYKSLEKEEPT
PGKPDKSGLQNLYNQYKDIKADGYTKDSWTAFDKARTEAEKVLTNEKATQAEIDAAKAAL
QAAYEGLAKDTTPEPEPQPNPNPTPGGNGGNSGNAGNSGNGSNTAVVTGDSANIAGYLMM
LLAAGGIAAVTFFRRKRVK

Enzyme Prediction     

No EC number prediction in MGYG000003984_00613.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH31	501	747	4.3e-57	0.5292740046838408
CBM32	1055	1160	1.5e-16	0.7741935483870968

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
cd06596	GH31_CPE1046	1.97e-147	280	718	1	334	Clostridium CPE1046-like. CPE1046 is an uncharacterized Clostridium perfringens protein with a glycosyl hydrolase family 31 (GH31) domain. The domain architecture of CPE1046 and its orthologs includes a C-terminal fibronectin type 3 (FN3) domain and a coagulation factor 5/8 type C domain in addition to the GH31 domain. Enzymes of the GH31 family possess a wide range of different hydrolytic activities including alpha-glucosidase (glucoamylase and sucrase-isomaltase), alpha-xylosidase, 6-alpha-glucosyltransferase, 3-alpha-isomaltosyltransferase and alpha-1,4-glucan lyase. All GH31 enzymes cleave a terminal carbohydrate moiety from a substrate that varies considerably in size, depending on the enzyme, and may be either a starch or a glycoprotein.
COG1501	YicI	6.41e-68	117	858	76	767	Alpha-glucosidase, glycosyl hydrolase family GH31 [Carbohydrate transport and metabolism].
pfam01055	Glyco_hydro_31	1.33e-57	493	747	211	442	Glycosyl hydrolases family 31. Glycosyl hydrolases are key enzymes of carbohydrate metabolism. Family 31 comprises of enzymes that are, or similar to, alpha- galactosidases.
cd06603	GH31_GANC_GANAB_alpha	2.37e-44	493	751	193	430	neutral alpha-glucosidase C, neutral alpha-glucosidase AB. This subgroup includes the closely related glycosyl hydrolase family 31 (GH31) isozymes, neutral alpha-glucosidase C (GANC) and the alpha subunit of heterodimeric neutral alpha-glucosidase AB (GANAB). Initially distinguished on the basis of differences in electrophoretic mobility in starch gel, GANC and GANAB have been shown to have other differences, including those of substrate specificity. GANC and GANAB are key enzymes in glycogen metabolism that hydrolyze terminal, non-reducing 1,4-linked alpha-D-glucose residues from glycogen in the endoplasmic reticulum. The GANC/GANAB family includes the alpha-glucosidase II (ModA) from Dictyostelium discoideum as well as the alpha-glucosidase II (GLS2, or ROT2 - Reversal of TOR2 lethality protein 2) from Saccharomyces cerevisiae.
cd08759	Type_III_cohesin_like	5.45e-40	1255	1424	1	167	Cohesin domain, interaction partner of dockerin. Bacterial cohesin domains bind to a complementary protein domain named dockerin, and this interaction is required for the formation of the cellulosome, a cellulose-degrading complex. Two specific calcium-dependent interactions between cohesin and dockerin appear to be essential for cellulosome assembly, type I and type II. This subfamily represents type III cohesins and closely related domains.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QNM10857.1	0.0	7	2105	7	2024
BBK61154.1	0.0	43	2096	39	2138
BCT46261.1	0.0	1	2105	1	2127
QWT17625.1	0.0	14	2038	24	2115
QUO30799.1	0.0	41	1400	23	1386

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
6M76_A	1.19e-240	41	1041	38	963	GH31alpha-N-acetylgalactosaminidase from Enterococcus faecalis [Enterococcus faecalis ATCC 10100],6M77_A GH31 alpha-N-acetylgalactosaminidase from Enterococcus faecalis in complex with N-acetylgalactosamine [Enterococcus faecalis ATCC 10100]
7F7R_A	6.28e-240	41	1041	38	963	ChainA, GH31 alpha-N-acetylgalactosaminidase [Enterococcus faecalis ATCC 10100]
7F7Q_A	1.71e-239	41	1041	38	963	ChainA, GH31 alpha-N-acetylgalactosaminidase [Enterococcus faecalis ATCC 10100]
7KBJ_A	3.24e-25	507	783	241	502	ChainA, Neutral alpha-glucosidase AB Trypsin-cleaved Fragment #3 [Mus musculus],7KBJ_C Chain C, Neutral alpha-glucosidase AB Trypsin-cleaved Fragment #3 [Mus musculus],7KBR_A Chain A, Neutral alpha-glucosidase AB Trypsin-cleaved Fragment #3 [Mus musculus],7KBR_C Chain C, Neutral alpha-glucosidase AB Trypsin-cleaved Fragment #3 [Mus musculus],7L9E_A Chain A, Neutral alpha-glucosidase AB Trypsin-cleaved Fragment #3 [Mus musculus],7L9E_C Chain C, Neutral alpha-glucosidase AB Trypsin-cleaved Fragment #3 [Mus musculus]
5F0E_A	7.34e-25	507	783	501	762	Murineendoplasmic reticulum alpha-glucosidase II [Mus musculus],5H9O_A Complex of Murine endoplasmic reticulum alpha-glucosidase II with D-Glucose [Mus musculus],5H9O_C Complex of Murine endoplasmic reticulum alpha-glucosidase II with D-Glucose [Mus musculus],5HJO_A Murine endoplasmic reticulum alpha-glucosidase II with bound substrate analogue [Mus musculus],5HJO_C Murine endoplasmic reticulum alpha-glucosidase II with bound substrate analogue [Mus musculus],5HJR_A Murine endoplasmic reticulum alpha-glucosidase II with bound covalent intermediate [Mus musculus],5HJR_C Murine endoplasmic reticulum alpha-glucosidase II with bound covalent intermediate [Mus musculus]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
Q9F234	1.03e-32	69	784	48	712	Alpha-glucosidase 2 OS=Bacillus thermoamyloliquefaciens OX=1425 PE=3 SV=1
Q9FN05	1.92e-31	477	787	533	822	Probable glucan 1,3-alpha-glucosidase OS=Arabidopsis thaliana OX=3702 GN=PSL5 PE=1 SV=1
B9F676	1.04e-28	511	784	560	817	Probable glucan 1,3-alpha-glucosidase OS=Oryza sativa subsp. japonica OX=39947 GN=Os03g0216600 PE=3 SV=1
Q8BVW0	6.82e-28	507	749	541	765	Neutral alpha-glucosidase C OS=Mus musculus OX=10090 GN=Ganc PE=1 SV=2
Q9P999	9.70e-28	329	783	181	658	Alpha-xylosidase OS=Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2) OX=273057 GN=xylS PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.000397	0.998812	0.000219	0.000209	0.000179	0.000150

TMHMM  Annotations     

start	end
13	32
2214	2233