logo
sublogo
You are browsing environment: HUMAN GUT
help

CAZyme Information: MGYG000003668_02448

You are here: Home > Sequence: MGYG000003668_02448

Basic Information | Genomic context | Full Sequence | Enzyme annotations |  CAZy signature domains |  CDD domains | CAZyme hits | PDB hits | Swiss-Prot hits | SignalP and Lipop annotations | TMHMM annotations

Basic Information help

Species UBA1777 sp900549485
Lineage Bacteria; Firmicutes_A; Clostridia; Oscillospirales; Oscillospiraceae; UBA1777; UBA1777 sp900549485
CAZyme ID MGYG000003668_02448
CAZy Family GH4
CAZyme Description Alpha-galactosidase
CAZyme Property
Protein Length CGC Molecular Weight Isoelectric Point
472 MGYG000003668_182|CGC1 52647.72 4.9676
Genome Property
Genome Assembly ID Genome Size Genome Type Country Continent
MGYG000003668 2556104 MAG Peru South America
Gene Location Start: 18122;  End: 19540  Strand: +

Full Sequence      Download help

MNKVAIIGAG  SLVFTKQLLN  DMFNTECMAG  TTYFLMGPTM  WKLEKMKQYA  QLLIEKNNID60
AEIICTTDRR  EALKDANFVI  LTFLVGGNAA  YKYDIEIPLK  YGVDQCIGDS  LGPGGVFRML120
RTAPVMAALG  KEVDELCPHA  YILNYVNPMG  AVCTTLSWCT  KAQVVGLCHG  VQTTLDLISG180
YTGVPKDEIE  YINAGINHMD  WFLTLEHNGM  DLYPILKEKM  EKPEYYKNEK  VRGELMRHCG240
YFMTESSGHL  SEYIPWFRSS  QEGLDTYCNE  PMFGGETGAA  LKFNMAVAEK  FKNTDVFSIE300
SGELEPRSKE  YCSYIIEAIM  TDRPFRFSGN  ISNKNGSIKN  LPLTATVEVP  VYADKYGFHP360
YNVGELPAHL  AAMNMSNISV  QELAARAGLD  GDTEMAFWAI  AMDPLTSTKL  TLKNCRKMAA420
EMLEAERKWL  PQFEGKTLKI  IEDVAIPEGT  VGVPVPTDPA  LAINERFNKL  AQ472

Enzyme Prediction      help

No EC number prediction in MGYG000003668_02448.

CAZyme Signature Domains help

Created with Snap234770941181411651882122362592833063303543774014244483176GH4
Family Start End Evalue family coverage
GH4 3 176 1.1e-53 0.9608938547486033

CDD Domains      download full data without filtering help

Created with Snap234770941181411651882122362592833063303543774014244481433PRK150763423GH4_alpha_glucosidase_galactosidase3433CelF3430GH4_P_beta_glucosidase3425GH4_glycoside_hydrolases
Cdd ID Domain E-Value qStart qEnd sStart sEnd Domain Description
PRK15076 PRK15076 3.37e-173 1 433 1 430
alpha-galactosidase; Provisional
cd05297 GH4_alpha_glucosidase_galactosidase 1.37e-164 3 423 2 423
Glycoside Hydrolases Family 4; Alpha-glucosidases and alpha-galactosidases. linked to 3D####ucture
COG1486 CelF 4.04e-116 3 433 5 439
Alpha-galactosidase/6-phospho-beta-glucosidase, family 4 of glycosyl hydrolase [Carbohydrate transport and metabolism].
cd05296 GH4_P_beta_glucosidase 4.32e-55 3 430 2 419
Glycoside Hydrolases Family 4; Phospho-beta-glucosidase. Some bacteria simultaneously translocate and phosphorylate disaccharides via the phosphoenolpyruvate-dependent phosphotransferase system (PEP-PTS). After translocation, these phospho-disaccharides may be hydrolyzed by the GH4 glycoside hydrolases such as the phospho-beta-glucosidases. Other organisms (such as archaea and Thermotoga maritima ) lack the PEP-PTS system, but have several enzymes normally associated with the PEP-PTS operon. The 6-phospho-beta-glucosidase from Thermotoga maritima hydrolylzes cellobiose 6-phosphate (6P) into glucose-6P and glucose, in an NAD+ and Mn2+ dependent fashion. The Escherichia coli 6-phospho-beta-glucosidase (also called celF) hydrolyzes a variety of phospho-beta-glucosides including cellobiose-6P, salicin-6P, arbutin-6P, and gentobiose-6P. Phospho-beta-glucosidases are part of the NAD(P)-binding Rossmann fold superfamily, which includes a wide variety of protein families including the NAD(P)-binding domains of alcohol dehydrogenases, tyrosine-dependent oxidoreductases, glyceraldehyde-3-phosphate dehydrogenases, formate/glycerate dehydrogenases, siroheme synthases, 6-phosphogluconate dehydrogenases, aminoacid dehydrogenases, repressor rex, and NAD-binding potassium channel domains, among others.
cd05197 GH4_glycoside_hydrolases 4.72e-55 3 425 2 425
Glycoside Hydrases Family 4. Glycoside hydrolases cleave glycosidic bonds to release smaller sugars from oligo- or polysaccharides. Some bacteria simultaneously translocate and phosphorylate disaccharides via the phosphoenolpyruvate-dependent phosphotransferase system (PEP-PTS). After translocation, these phospho-disaccharides may be hydrolyzed by GH4 glycoside hydrolases. Other organisms (such as archaea and Thermotoga maritima) lack the PEP-PTS system, but have several enzymes normally associated with the PEP-PTS operon. GH4 family members include 6-phospho-beta-glucosidases, 6-phospho-alpha-glucosidases, alpha-glucosidases/alpha-glucuronidases (only from Thermotoga), and alpha-galactosidases. They require two cofactors, NAD+ and a divalent metal (Mn2+, Ni2+, Mg2+), for activity. Some also require reducing conditions. GH4 glycoside hydrolases are part of the NAD(P)-binding Rossmann fold superfamily, which includes a wide variety of protein families including the NAD(P)-binding domains of alcohol dehydrogenases, tyrosine-dependent oxidoreductases, glyceraldehyde-3-phosphate dehydrogenases, formate/glycerate dehydrogenases, siroheme synthases, 6-phosphogluconate dehydrogenases, aminoacid dehydrogenases, repressor rex, and NAD-binding potassium channel domains, among others.

CAZyme Hits      help

Created with Snap234770941181411651882122362592833063303543774014244481470AEF83088.1|GH41472QXD23685.1|GH41472QXD27757.1|GH41472QNK60298.1|GH41472QTH40102.1|GH4
Hit ID E-Value Query Start Query End Hit Start Hit End
AEF83088.1 1.26e-246 1 470 1 469
QXD23685.1 7.87e-195 1 472 1 471
QXD27757.1 7.87e-195 1 472 1 471
QNK60298.1 1.48e-194 1 472 1 470
QTH40102.1 4.21e-194 1 472 1 470

PDB Hits      download full data without filtering help

Created with Snap2347709411814116518821223625928330633035437740142444834335C3M_A34331S6Y_A34053FEF_A34081OBB_A33593U95_A
Hit ID E-Value Query Start Query End Hit Start Hit End Description
5C3M_A 1.25e-44 3 433 6 437
Crystalstructure of Gan4C, a GH4 6-phospho-glucosidase from Geobacillus stearothermophilus [Geobacillus stearothermophilus],5C3M_B Crystal structure of Gan4C, a GH4 6-phospho-glucosidase from Geobacillus stearothermophilus [Geobacillus stearothermophilus],5C3M_C Crystal structure of Gan4C, a GH4 6-phospho-glucosidase from Geobacillus stearothermophilus [Geobacillus stearothermophilus],5C3M_D Crystal structure of Gan4C, a GH4 6-phospho-glucosidase from Geobacillus stearothermophilus [Geobacillus stearothermophilus]
1S6Y_A 1.73e-41 3 433 9 440
2.3Acrystal structure of phospho-beta-glucosidase [Geobacillus stearothermophilus]
3FEF_A 2.58e-36 3 405 7 420
Crystalstructure of putative glucosidase lplD from bacillus subtilis [Bacillus subtilis],3FEF_B Crystal structure of putative glucosidase lplD from bacillus subtilis [Bacillus subtilis],3FEF_C Crystal structure of putative glucosidase lplD from bacillus subtilis [Bacillus subtilis],3FEF_D Crystal structure of putative glucosidase lplD from bacillus subtilis [Bacillus subtilis]
1OBB_A 9.40e-32 3 408 5 453
alpha-glucosidaseA, AglA, from Thermotoga maritima in complex with maltose and NAD+ [Thermotoga maritima MSB8],1OBB_B alpha-glucosidase A, AglA, from Thermotoga maritima in complex with maltose and NAD+ [Thermotoga maritima MSB8]
3U95_A 4.09e-25 3 359 2 392
Crystalstructure of a putative alpha-glucosidase from Thermotoga neapolitana [Thermotoga neapolitana DSM 4359],3U95_B Crystal structure of a putative alpha-glucosidase from Thermotoga neapolitana [Thermotoga neapolitana DSM 4359],3U95_C Crystal structure of a putative alpha-glucosidase from Thermotoga neapolitana [Thermotoga neapolitana DSM 4359],3U95_D Crystal structure of a putative alpha-glucosidase from Thermotoga neapolitana [Thermotoga neapolitana DSM 4359],3U95_E Crystal structure of a putative alpha-glucosidase from Thermotoga neapolitana [Thermotoga neapolitana DSM 4359],3U95_F Crystal structure of a putative alpha-glucosidase from Thermotoga neapolitana [Thermotoga neapolitana DSM 4359]

Swiss-Prot Hits      download full data without filtering help

Created with Snap234770941181411651882122362592833063303543774014244483433sp|Q9X4Y0|AGAL_RHIME1434sp|O34645|AGAL_BACSU3431sp|P30877|AGAL_SALTY3431sp|P06720|AGAL_ECOLI4430sp|I3VRU1|LPLD_THESW
Hit ID E-Value Query Start Query End Hit Start Hit End Description
Q9X4Y0 3.61e-87 3 433 5 442
Alpha-galactosidase OS=Rhizobium meliloti (strain 1021) OX=266834 GN=melA PE=3 SV=1
O34645 1.70e-85 1 434 1 432
Alpha-galactosidase OS=Bacillus subtilis (strain 168) OX=224308 GN=melA PE=1 SV=1
P30877 8.57e-77 3 431 6 446
Alpha-galactosidase OS=Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) OX=99287 GN=melA PE=3 SV=2
P06720 6.63e-76 3 431 6 446
Alpha-galactosidase OS=Escherichia coli (strain K12) OX=83333 GN=melA PE=1 SV=1
I3VRU1 7.04e-45 4 430 13 458
Alpha-galacturonidase OS=Thermoanaerobacterium saccharolyticum (strain DSM 8691 / JW/SL-YS485) OX=1094508 GN=Tsac_0200 PE=1 SV=1

SignalP and Lipop Annotations help

This protein is predicted as OTHER

Other SP_Sec_SPI LIPO_Sec_SPII TAT_Tat_SPI TATLIP_Sec_SPII PILIN_Sec_SPIII
1.000057 0.000000 0.000000 0.000000 0.000000 0.000000

TMHMM  Annotations      help

There is no transmembrane helices in MGYG000003668_02448.