CAZyme Information: MGYG000003396_01857

Basic Information

• Species: Clostridium sp900766315
• Lineage: Bacteria; Firmicutes_A; Clostridia; Clostridiales; Clostridiaceae; Clostridium; Clostridium sp900766315
• CAZyme ID: MGYG000003396_01857
• CAZy Family: GH101
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
1908	MGYG000003396_100|CGC1	210967.96	4.3416

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000003396	3926186	MAG	United States	North America

• Gene Location: Start: 1753; End: 7479 Strand: -

Full Sequence      

MGKDIKRRAMSKRLAVLLASVVVVNQVTSVIPVNATGNNVNDISSGSAVVLNDEVYTLNF
DETDKGQPNWHDIQGSGQRIIENGQLKITRDTANNNFVFYDQNAPMLADGEVETEFTFDG
GTSRFGVVVRPSTDGNFLFVGYNDNGKWLIESSTAWKDDIAGPTLQEGQTYKLKVRFEGK
KITIWVDGEQIFSEVVELANMPTSQGYGVGFRTWYDQKTVNVNYLKAGAVGSITDEIGDK
TIESIEEVNVKTEQYIRPVMPTTVAVTYDDGTKGNIPVTWDTISKDKYAEVGTFTVEGTV
EGTDVKAVANITVTESSTGLVPPDVEVEELRLESQKMTAVLDNNFPRVIRYEWKSDGAVL
TGTEDQLYIAEINNTPYVPTVKGKVSGDKVKYTMTFDEIDVVIKLEMSFTKDDVLRMEVT
EIKEKGDFLVKTLNFPGHSLASVKDTENGQLAGVSTQGNRNDVKEEFSSVADKALGSYSK
IYGMINNDQFAVTLNNNLIESGNKAIISVEQEDGYKKASIANGKWTYRESLKETAAQVKF
IGQIAGVDITEENAADGTQVISQSNNSINKYMGRDFEAVYCGLGSVEDFAGKDLTGKVAI
VDRGTLTFLEKAQNAGAAGAAYVICINNTSGVVPAQGENDVLNMIGISQETGEAIKANLS
EDGTTTINLNLLSNSSNDVVPGDPEELPWSEIMIARDENGNGKVTWQDAAIKYRDNMEEV
QGADYVRNSFSYIPFNIGSLAQMPFIKTADYIKKLSNYTDGFGQLVLHKGFQAEGHDDAK
ADIGGHTGIRQGGKDDLNELIEIGKDYNARIGVHLNLTEYHLDAFQFKVENLRPGFEEGW
NWLDQAYWVDKRSDILSGELARRFELLEADHPDLSWIYIDVYDGVGWNSKQLANEIHSRG
WMAATEFNGPLYEDVAWTHWGADPAYPNAGNSSKISRFIRNQEQDVYMSDDLLKGGKHML
ASGWGTRHDLEGFYAIETFYNQTLPSKYMQHFEIMEWEDNGTEGFVKFKGGLESKRENGY
NNMYQDGKLIASTAQDTINDRGIGKTTLFLPWTWEQLEDEPENKVYHWNPYGGETTWDLP
TGWEGTESVKIYELSDLGRTFVEEIQVVDGKVTIDAKQNTPYVVFQEKVNEDRIDSWGEG
SLISDPGFDSQSFDHWNVESTSDNMDHIKIENETVDRRKGNDVFVVEGIEKVDPTLIPQS
EMTATATSSQSGEGADKAIDGNESTLWHTSYAGVTGPQNITVDLGGKYEINKVTCLPRQS
GNNGKITKYELQVSTNGQDFTKVAEGDWNTSSDLKTINFDAVEATHIRLVANEYVNGFAS
MAELNVHKNVEIPDEEKVFGEGANISQEITDLVPGETYIASVWVKNSGNREVTLGVDCGG
KETTSTIDRATTRMNTGEGLKWHGERLTRMRVEFTVPEGVTTAKLYVDIPRSEEATRVML
DDFRIWTNPQESVDTNRDGYVIYEDFENVDEGHGPFYMGQNLGTDNRTHYAEKNPEGGQY
MNWVISDRFSLKTNQQAGTTGHILLTDQSTLQLEPNKTYELGFKYTNKVDNLYTVSVNSA
TAGTLFEEVITPGEVIGIPTDGPGYSREVKEFKYEFTTGDAVDYYLAINKGAGYDELVID
DLYIKDLSEEEVASNKKHLQIAVEMAKEITDAELENVVPAVVEEFKAALANAEAVLGNEK
ATQSEVDKAFDRLSEAMQMLSFVKGDKEALQSFIDKIEKLDSKEYIASTWNKLQEQLDAS
KAVIADPNAMEKEVMESYDKLVRSFLELRLKPSKDKLQDLINKADALDSSKYTNKSWKAL
ENTLLLAKSVLSNDDATEEEVTNAEANLQKAIDGLILADAGNGDDNNDNGNNGNNGNGGS
NGNGGNGTTTKPSKPGKLPQTGGTNALVTLLTGLTAIGGGFLFKRKNK

Enzyme Prediction     

EC	3.2.1.97

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH101	630	1126	1.6e-115	0.7468175388967468
CBM32	1203	1324	1.4e-26	0.9354838709677419

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
pfam12905	Glyco_hydro_101	1.08e-103	707	974	1	273	Endo-alpha-N-acetylgalactosaminidase. Virulence of pathogenic organisms such as the Gram-positive Streptococcus pneumoniae is largely determined by the ability to degrade host glycoproteins and to metabolize the resultant carbohydrates. This family is the enzymatic region, EC:3.2.1.97, of the cell surface proteins that specifically cleave Gal-beta-1,3-GalNAc-alpha-Ser/Thr (T-antigen, galacto-N-biose), the core 1 type O-linked glycan common to mucin glycoproteins. This reaction is exemplified by the S. pneumoniae protein Endo-alpha-N-acetylgalactosaminidase, where Asp764 is the catalytic nucleophile-base and Glu796 the catalytic proton donor.
cd14244	GH_101_like	3.90e-97	722	999	4	298	Endo-a-N-acetylgalactosaminidase and related glcyosyl hydrolases. This family contains the enzymatically active domain of cell surface proteins that specifically cleave Gal-beta-1,3-GalNAc-alpha-Ser/Thr (T-antigen, galacto-N-biose), the core 1 type O-linked glycan common to mucin glycoproteins (EC:3.2.1.97). It has been classified as glycosyl hydrolase family 101 in the Cazy resource. Virulence of pathogenic organisms such as the Gram-positive Streptococcus pneumoniae and other commensal human bacteria is largely determined by their ability to degrade host glycoproteins and to metabolize the resultant carbohydrates.
pfam18080	Gal_mutarotas_3	1.37e-48	332	532	1	213	Galactose mutarotase-like fold domain. This domain is found in endo-alpha-N-acetylgalactosaminidase present in Streptococcus pneumoniae. Endo-alpha-N-acetylgalactosaminidase is a cell surface-anchored glycoside hydrolase involved in the breakdown of mucin type O-linked glycans. The domain, known as domain 2, exhibits strong structural similarlity to the galactose mutarotase-like fold but lacks the active site residues. Domains, found in a number of glycoside hydrolases, structurally similar to domain 2 confer stability to the multidomain architectures.
pfam17451	Glyco_hyd_101C	2.38e-25	984	1100	3	111	Glycosyl hydrolase 101 beta sandwich domain. Virulence of pathogenic organisms such as the Gram-positive Streptococcus pneumoniae is largely determined by the ability to degrade host glycoproteins and to metabolize the resultant carbohydrates. This family is the enzymatic region, EC:3.2.1.97, of the cell surface proteins that specifically cleave Gal-beta-1,3-GalNAc-alpha-Ser/Thr (T-antigen, galacto-N-biose), the core 1 type O-linked glycan common to mucin glycoproteins. This reaction is exemplified by a S. pneumoniae protein, where Asp764 is the catalytic nucleophile-base and Glu796 the catalytic proton donor. This domain represents C-terminal the beta sandwich domain.
pfam00754	F5_F8_type_C	3.09e-24	1203	1324	1	127	F5/8 type C domain. This domain is also known as the discoidin (DS) domain family.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
AYE33294.1	0.0	7	1631	11	1484
QAS61465.1	0.0	7	1631	11	1484
ATD54766.1	0.0	4	1631	6	1487
QBJ75049.1	0.0	4	1631	6	1487
SLK16230.1	0.0	4	1631	6	1487

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
2ZXQ_A	3.52e-83	317	1709	11	1211	Crystalstructure of endo-alpha-N-acetylgalactosaminidase from Bifidobacterium longum (EngBF) [Bifidobacterium longum]
6QEP_A	9.38e-83	332	1712	12	1199	EngBFDARPin Fusion 4b H14 [Bifidobacterium longum]
6QEV_B	9.57e-83	332	1712	12	1199	EngBFDARPin Fusion 4b B6 [Bifidobacterium longum]
6QFK_A	9.57e-83	332	1712	12	1199	EngBFDARPin Fusion 4b G10 [Bifidobacterium longum]
6SH9_B	9.57e-83	332	1712	12	1199	EngBFDARPin Fusion 4b D12 [Bifidobacterium longum subsp. longum JCM 1217]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
A9WNA0	8.69e-81	685	1158	276	745	Putative endo-alpha-N-acetylgalactosaminidase OS=Renibacterium salmoninarum (strain ATCC 33209 / DSM 20767 / JCM 11484 / NBRC 15589 / NCIMB 2235) OX=288705 GN=RSal33209_1326 PE=3 SV=2
Q8DR60	4.21e-68	679	1557	559	1332	Endo-alpha-N-acetylgalactosaminidase OS=Streptococcus pneumoniae (strain ATCC BAA-255 / R6) OX=171101 GN=spr0328 PE=1 SV=1
Q2MGH6	1.65e-67	679	1557	559	1332	Endo-alpha-N-acetylgalactosaminidase OS=Streptococcus pneumoniae serotype 4 (strain ATCC BAA-334 / TIGR4) OX=170187 GN=SP_0368 PE=1 SV=1
P29767	3.10e-22	1196	1369	50	233	Sialidase OS=Clostridium septicum OX=1504 PE=3 SV=1
Q0TR53	4.64e-21	1184	1358	612	808	O-GlcNAcase NagJ OS=Clostridium perfringens (strain ATCC 13124 / DSM 756 / JCM 1290 / NCIMB 6125 / NCTC 8237 / Type A) OX=195103 GN=nagJ PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.000300	0.999006	0.000170	0.000191	0.000161	0.000142

TMHMM  Annotations     

start	end
1881	1903