CAZyme Information: MGYG000003334_01528

Basic Information

• Species: Lactobacillus gallinarum
• Lineage: Bacteria; Firmicutes; Bacilli; Lactobacillales; Lactobacillaceae; Lactobacillus; Lactobacillus gallinarum
• CAZyme ID: MGYG000003334_01528
• CAZy Family: GT8
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
278		32858.91	8.4009

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000003334	1842627	MAG	China	Asia

• Gene Location: Start: 6056; End: 6892 Strand: +

Full Sequence      

MNILFCGDENAEDGVLISTLSLIKNSGAKKLHLYILTMDAHSDERKYHSFSMQAANYIRS
LLTQANPNNTLQLIDCTELFEKQPPTVNMNTRFTPYAMLRLFADQLPEIPDRILYLDDDI
IIRSDITEFYQQDMTGIELAGVLDYWGKFFFHNVKTKHVFDYLNSGVLLLNMPKIKETDL
FAKVRQMMQTKKMFLPDQSAINKLAVAKRVFPRYYNEQYKLQKNTKIQHFTTSFRFWPYF
HTQTVKPWDVDRVHSVLHLHEYDDILTEYLKIRHNLKK

Enzyme Prediction     

No EC number prediction in MGYG000003334_01528.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GT8	2	249	2.2e-36	0.867704280155642

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
cd04194	GT8_A4GalT_like	1.33e-33	1	237	1	246	A4GalT_like proteins catalyze the addition of galactose or glucose residues to the lipooligosaccharide (LOS) or lipopolysaccharide (LPS) of the bacterial cell surface. The members of this family of glycosyltransferases catalyze the addition of galactose or glucose residues to the lipooligosaccharide (LOS) or lipopolysaccharide (LPS) of the bacterial cell surface. The enzymes exhibit broad substrate specificities. The known functions found in this family include: Alpha-1,4-galactosyltransferase, LOS-alpha-1,3-D-galactosyltransferase, UDP-glucose:(galactosyl) LPS alpha1,2-glucosyltransferase, UDP-galactose: (glucosyl) LPS alpha1,2-galactosyltransferase, and UDP-glucose:(glucosyl) LPS alpha1,2-glucosyltransferase. Alpha-1,4-galactosyltransferase from N. meningitidis adds an alpha-galactose from UDP-Gal (the donor) to a terminal lactose (the acceptor) of the LOS structure of outer membrane. LOSs are virulence factors that enable the organism to evade the immune system of host cells. In E. coli, the three alpha-1,2-glycosyltransferases, that are involved in the synthesis of the outer core region of the LPS, are all members of this family. The three enzymes share 40 % of sequence identity, but have different sugar donor or acceptor specificities, representing the structural diversity of LPS.
COG1442	RfaJ	2.45e-14	1	278	3	283	Lipopolysaccharide biosynthesis protein, LPS:glycosyltransferase [Cell wall/membrane/envelope biogenesis].
pfam01501	Glyco_transf_8	2.59e-11	2	237	1	248	Glycosyl transferase family 8. This family includes enzymes that transfer sugar residues to donor molecules. Members of this family are involved in lipopolysaccharide biosynthesis and glycogen synthesis. This family includes Lipopolysaccharide galactosyltransferase, lipopolysaccharide glucosyltransferase 1, and glycogenin glucosyltransferase.
PRK15171	PRK15171	2.95e-07	1	221	26	242	lipopolysaccharide 3-alpha-galactosyltransferase.
cd00505	Glyco_transf_8	9.87e-07	1	237	1	244	Members of glycosyltransferase family 8 (GT-8) are involved in lipopolysaccharide biosynthesis and glycogen synthesis. Members of this family are involved in lipopolysaccharide biosynthesis and glycogen synthesis. GT-8 comprises enzymes with a number of known activities: lipopolysaccharide galactosyltransferase, lipopolysaccharide glucosyltransferase 1, glycogenin glucosyltransferase, and N-acetylglucosaminyltransferase. GT-8 enzymes contains a conserved DXD motif which is essential in the coordination of a catalytic divalent cation, most commonly Mn2+.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
ALI51768.1	5.47e-196	1	278	1	278
AFR21129.1	7.77e-196	1	278	1	278
QPB50916.1	7.77e-196	1	278	1	278
QAU32014.1	7.77e-196	1	278	1	278
AZK91838.1	7.77e-196	1	278	1	278

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
6U4B_A	2.58e-08	62	211	353	520	ChainA, WbbM protein [Klebsiella pneumoniae]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
P27129	7.00e-12	1	226	28	245	Lipopolysaccharide 1,2-glucosyltransferase OS=Escherichia coli (strain K12) OX=83333 GN=rfaJ PE=3 SV=2
P43974	3.69e-09	1	183	39	209	Putative glycosyltransferase HI_0258 OS=Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd) OX=71421 GN=HI_0258 PE=3 SV=2
A0A0H2URH2	5.03e-06	68	202	56	180	Glycosyltransferase GlyF OS=Streptococcus pneumoniae serotype 4 (strain ATCC BAA-334 / TIGR4) OX=170187 GN=glyF PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000070	0.000000	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

There is no transmembrane helices in MGYG000003334_01528.