CAZyme Information: MGYG000003074_00544

Basic Information

• Species: Clostridium_Q sp000435655
• Lineage: Bacteria; Firmicutes_A; Clostridia; Lachnospirales; Lachnospiraceae; Clostridium_Q; Clostridium_Q sp000435655
• CAZyme ID: MGYG000003074_00544
• CAZy Family: GT51
• CAZyme Description: Monofunctional biosynthetic peptidoglycan transglycosylase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
843	MGYG000003074_13|CGC1	94198.05	4.6632

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000003074	2527682	MAG	China	Asia

• Gene Location: Start: 13721; End: 16252 Strand: +

Full Sequence      

MDRERNERGHTRGRQGQKTPGPADAQKRQDRKEAKRLQERRAEEAVQQELGEQIQSILEE
ETGEQEGARQQKAAGPDPLSREPAARTEEEAGRRRRPARQKKGWRRLWKRFFLMFLLILF
VAAAGCGFLGWKFYTICKEEVDGLVANSTEADFLPGQASYFYDVNGNVLAKISGDEDSQY
LPYEEIPQDAVNAFIAIEDRTFWENCGVDFKGILRVGINFILTKGEEVHGASTITQQLAR
NRYLTRDVSIKRKVKEILIAMDLTEKYSKEQIMEFYVNDINYANTYYGLQAAAKGYFGKD
ADELSLSQIAYLCAIPNSPSYYNPYRHPDHALTRRDKILGDMLTMGYITQEEHDAAVAEE
ITVTGNSYEMRNYETTYAIDCAVRWLMEREGFSFEYGFSSYDDYKSYQAAYEESYAAAKD
ELYTGGYRIYTSLDPAKQEILQSAVDQVLSFDAETADNGIYALQGAAVAVNNDTGLVEAI
VGGRSQETSTYTLNRAFQSFRQPGSSIKPLIVYAPALEEGYASFSSVKDINVDAAKEPKA
DVASLPGNWITLRQAVEKSRNGAAWWLFNEVTPEEGLSYLTQMQFDKIVPDDYNMAASLG
GFTYGVTAEEMAGAFSALENGGVYREPDCIVRMENLAGEDIYTGREPKEVYSQQTAAVMV
DVMKGVISRGTARGMGWKSGIEAAGKTGTTNNSKDGWFCGMTPYYTVTVWVGYDTPRTLS
SLYGSSYPASIWKQAMEGLTAGLPEAAFAAPEAEKEDIKKEGRGEYLEGYDDSHVLSPNY
TVGDYRRDHALADEAQKYIDQMAGASPEEQERLRKKAESAINRIYGRTLKGQMEEVLAGA
GKS

Enzyme Prediction     

No EC number prediction in MGYG000003074_00544.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GT51	166	342	1.5e-61	0.9830508474576272

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
TIGR02074	PBP_1a_fam	6.55e-165	179	737	3	526	penicillin-binding protein, 1A family. Bacterial that synthesize a cell wall of peptidoglycan (murein) generally have several transglycosylases and transpeptidases for the task. This family consists of bifunctional transglycosylase/transpeptidase penicillin-binding proteins (PBP). In the Proteobacteria, this family includes PBP 1A but not the paralogous PBP 1B (TIGR02071). This family also includes related proteins, often designated PBP 1A, from other bacterial lineages. [Cell envelope, Biosynthesis and degradation of murein sacculus and peptidoglycan]
COG0744	MrcB	3.02e-160	157	743	54	600	Membrane carboxypeptidase (penicillin-binding protein) [Cell wall/membrane/envelope biogenesis].
COG5009	MrcA	5.93e-133	151	736	41	715	Membrane carboxypeptidase/penicillin-binding protein [Cell wall/membrane/envelope biogenesis].
TIGR02071	PBP_1b	1.37e-79	176	740	145	683	penicillin-binding protein 1B. Bacterial that synthesize a cell wall of peptidoglycan (murein) generally have several transglycosylases and transpeptidases for the task. This family consists of a particular bifunctional transglycosylase/transpeptidase in E. coli and other Proteobacteria, designated penicillin-binding protein 1B. [Cell envelope, Biosynthesis and degradation of murein sacculus and peptidoglycan]
pfam00912	Transgly	1.44e-71	165	343	1	177	Transglycosylase. The penicillin-binding proteins are bifunctional proteins consisting of transglycosylase and transpeptidase in the N- and C-terminus respectively. The transglycosylase domain catalyzes the polymerization of murein glycan chains.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
CBK77227.1	1.15e-258	1	546	1	573
BAK46854.1	1.45e-236	94	748	50	715
CBK80882.1	5.03e-234	99	829	55	789
BCK01696.1	6.03e-198	111	764	66	720
BCD34019.1	4.42e-188	113	739	33	658

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
4OON_A	6.58e-72	162	751	24	734	Crystalstructure of PBP1a in complex with compound 17 ((4Z,8S,11E,14S)-5-(2-amino-1,3-thiazol-4-yl)-14-(5,6-dihydroxy-1,3-dioxo-1,3-dihydro-2H-isoindol-2-yl)-8-formyl-2-methyl-6-oxo-3,10-dioxa-4,7,11-triazatetradeca-4,11-diene-2,12,14-tricarboxylic acid) [Pseudomonas aeruginosa PAO1]
3UDF_A	9.52e-66	162	746	24	722	ChainA, Penicillin-binding protein 1a [Acinetobacter baumannii],3UDF_B Chain B, Penicillin-binding protein 1a [Acinetobacter baumannii],3UDI_A Chain A, Penicillin-binding protein 1a [Acinetobacter baumannii],3UDI_B Chain B, Penicillin-binding protein 1a [Acinetobacter baumannii],3UDX_A Chain A, Penicillin-binding protein 1a [Acinetobacter baumannii],3UDX_B Chain B, Penicillin-binding protein 1a [Acinetobacter baumannii],3UE0_A Chain A, Penicillin-binding protein 1a [Acinetobacter baumannii],3UE0_B Chain B, Penicillin-binding protein 1a [Acinetobacter baumannii],3UE1_A Chain A, Penicillin-binding protein 1a [Acinetobacter baumannii],3UE1_B Chain B, Penicillin-binding protein 1a [Acinetobacter baumannii]
7U4H_A	8.68e-58	142	751	4	772	ChainA, Penicillin-binding protein 1A (Pbp1a) [Chlamydia trachomatis D/UW-3/CX],7U4H_B Chain B, Penicillin-binding protein 1A (Pbp1a) [Chlamydia trachomatis D/UW-3/CX]
3ZG8_B	2.54e-53	262	714	2	433	CrystalStructure of Penicillin Binding Protein 4 from Listeria monocytogenes in the Ampicillin bound form [Listeria monocytogenes],3ZG9_B Crystal Structure of Penicillin-Binding Protein 4 from Listeria monocytogenes in the Cefuroxime bound form [Listeria monocytogenes],3ZGA_B Crystal Structure of Penicillin-Binding Protein 4 from Listeria monocytogenes in the Carbenicillin bound form [Listeria monocytogenes]
3DWK_A	2.54e-53	162	741	11	590	ChainA, Penicillin-binding protein 2 [Staphylococcus aureus subsp. aureus COL],3DWK_B Chain B, Penicillin-binding protein 2 [Staphylococcus aureus subsp. aureus COL],3DWK_C Chain C, Penicillin-binding protein 2 [Staphylococcus aureus subsp. aureus COL],3DWK_D Chain D, Penicillin-binding protein 2 [Staphylococcus aureus subsp. aureus COL]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
P40750	6.82e-95	157	737	54	621	Penicillin-binding protein 4 OS=Bacillus subtilis (strain 168) OX=224308 GN=pbpD PE=1 SV=2
P38050	9.24e-87	158	756	48	612	Penicillin-binding protein 1F OS=Bacillus subtilis (strain 168) OX=224308 GN=pbpF PE=2 SV=2
O66874	1.03e-77	155	751	41	679	Penicillin-binding protein 1A OS=Aquifex aeolicus (strain VF5) OX=224324 GN=mrcA PE=1 SV=1
Q8XJ01	9.58e-75	125	725	34	652	Penicillin-binding protein 1A OS=Clostridium perfringens (strain 13 / Type A) OX=195102 GN=pbpA PE=3 SV=1
Q0TNZ8	7.29e-73	125	725	34	652	Penicillin-binding protein 1A OS=Clostridium perfringens (strain ATCC 13124 / DSM 756 / JCM 1290 / NCIMB 6125 / NCTC 8237 / Type A) OX=195103 GN=pbpA PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000056	0.000000	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

start	end
111	131