CAZyme Information: MGYG000003068_01298

Basic Information

• Species: UMGS1388 sp900551345
• Lineage: Bacteria; Firmicutes_A; Clostridia; Monoglobales; UMGS1388; UMGS1388; UMGS1388 sp900551345
• CAZyme ID: MGYG000003068_01298
• CAZy Family: GH5
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
1334		147787.55	4.2752

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000003068	1753072	MAG	United States	North America

• Gene Location: Start: 182; End: 4186 Strand: +

Full Sequence      

MKRFAAIFVIFVMITAMLPAAFAQDTADPNLSRQLMVNGNRIVYADDPDTAVRLAGVNIP
SLEWGFKGEHIFESLTEGCDGWNANIVRLPLSPTRWFDETEGAAYQEIVAKAAKGVCARG
KYLLLDCHTYVMPTQEVYDFWMDVCDKYGNNPAVLFGLCNEPHDIKGMLEDADKSQWELW
HSGGIISYNGEAALGIGHQTLVEAIRDKGAKNILVAAGLSWGYDLTGVANGYALVDCGSN
NDTSKTGYGIMYDSHIYPSKGLESSWDKAAGCVRSFAPLLIGEFGWDSSDKNISNDTSVY
NLWMPQLLDWIDDTDGKYGVPANWTAWCFHPSATPKVITSWKYTPTYYNGKYVKERLLSY
PDTGCYLDRTYTNDFEPDMFTSYTAVKNGSETGHKYTLKNNAMELYYKRGANSQYVGYSM
NLPSDWCLEGLQRIELDITPHSDAISNGMMLDIGFRGSDTELWGKKVTLEGTDTMHISIP
VNELENLAPSIKSDDKFTYGIIGLYIGAGNASKGTVTVDNIKIYTEADPVISMPEPYQRP
EGNPQYLFDNNSDNTSLTVTKNDNGPGSSQGDYFTTTVEDGVGYEDSRGIHIKYNRINGI
WGGFSQLSYPAGSDFSDAVYWSFMLKGNGVQQQVSIKVKSGTATIVLPKDDTDWHQYIFR
LSQVGCMAPEEITYMQFHADNKLESEFWADNIQITKDYPTVFVEPDDVIFENGFEDSLVK
FNPIDTASSSVAGDYFDDGYNSQLAYKLDFNNDSSDEGATAEFKIPSDWTVKDTDTFTFD
AKTTSSEPVDIQVGLLDKVGIAAYTGSTHTVTDEWQRFTVPIYDYKLNDVAIVNSRITGI
HVKSLKIGESSILIDNISFSNCEVPPPVAHSVSYVNNFDDDVYGAYTSVNCDETNYINAA
VTDGAGYENTAGLVIDFAGKQSSSRYIDITGLPEDWDFSKAKYASLMVKGANPVVSGSYQ
TNGNAFVMEFYYDYVKDDGSAASVKTATVNFDAQRDVWSNIITSIVPATANTEYLKGTNR
IKIYTTRSMTGSVIIDNLGFYDMKPERVVPVGGATFTETFDSSSMLDLKFEGGYYTDPDN
YLYKHDSGGYSHWGFTLQCAHETPGVQSPQSGTDYVQASLPMSWELFRTTHLTFKARLNQ
TWRTKNFDTVFTGLPEGGLTETTMTVSLLDSDMNEYSQKIYIDSPSWKDYTVALSDFVNA
QGQSIDLTDVKYIRLYADENLTQSGFNMDNLSFITMNPEITSISVTCAGQPVTDTLVSGK
AVVQCSASGEAMPMTLFAVLYKNNKVYTVSSSAFNGGEASLEIEIPENPVEYTLKVFVWS
DNMQPVSGGYLCIQ

Enzyme Prediction     

No EC number prediction in MGYG000003068_01298.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH5	46	366	1e-33	0.9418181818181818

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
pfam00150	Cellulase	5.27e-16	80	331	34	270	Cellulase (glycosyl hydrolase family 5).

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QGT50938.1	1.40e-138	52	992	2	972
AHF90980.1	2.48e-59	19	357	226	563
QGT50946.1	1.49e-58	1	697	1	708
ADR64665.1	4.28e-58	35	346	607	898
QIF03557.1	2.06e-57	23	357	397	727

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
4HTY_A	1.61e-20	31	346	45	344	CrystalStructure of a metagenome-derived cellulase Cel5A [uncultured bacterium],4HU0_A Crystal Structure of a metagenome-derived cellulase Cel5A in complex with cellotetraose [uncultured bacterium]
6GJF_A	4.19e-10	34	357	12	300	Ancestralendocellulase Cel5A [synthetic construct],6GJF_B Ancestral endocellulase Cel5A [synthetic construct],6GJF_C Ancestral endocellulase Cel5A [synthetic construct],6GJF_D Ancestral endocellulase Cel5A [synthetic construct],6GJF_E Ancestral endocellulase Cel5A [synthetic construct],6GJF_F Ancestral endocellulase Cel5A [synthetic construct]
3PZT_A	9.18e-10	34	358	36	325	Structureof the endo-1,4-beta-glucanase from Bacillus subtilis 168 with manganese(II) ion [Bacillus subtilis subsp. subtilis str. 168],3PZT_B Structure of the endo-1,4-beta-glucanase from Bacillus subtilis 168 with manganese(II) ion [Bacillus subtilis subsp. subtilis str. 168],3PZU_A P212121 crystal form of the endo-1,4-beta-glucanase from Bacillus subtilis 168 [Bacillus subtilis subsp. subtilis str. 168],3PZU_B P212121 crystal form of the endo-1,4-beta-glucanase from Bacillus subtilis 168 [Bacillus subtilis subsp. subtilis str. 168],3PZV_A C2 crystal form of the endo-1,4-beta-glucanase from Bacillus subtilis 168 [Bacillus subtilis subsp. subtilis str. 168],3PZV_B C2 crystal form of the endo-1,4-beta-glucanase from Bacillus subtilis 168 [Bacillus subtilis subsp. subtilis str. 168],3PZV_C C2 crystal form of the endo-1,4-beta-glucanase from Bacillus subtilis 168 [Bacillus subtilis subsp. subtilis str. 168],3PZV_D C2 crystal form of the endo-1,4-beta-glucanase from Bacillus subtilis 168 [Bacillus subtilis subsp. subtilis str. 168]
4XZW_A	1.09e-09	34	325	11	265	Endo-glucanasechimera C10 [uncultured bacterium]
1LF1_A	1.87e-07	34	325	11	263	CrystalStructure of Cel5 from Alkalophilic Bacillus sp. [Bacillus subtilis]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
P10475	9.34e-11	1	456	1	439	Endoglucanase OS=Bacillus subtilis (strain 168) OX=224308 GN=eglS PE=1 SV=1
P07983	1.13e-09	1	363	1	335	Endoglucanase OS=Bacillus subtilis OX=1423 GN=bglC PE=3 SV=2
P06565	2.59e-09	1	325	1	289	Endoglucanase B OS=Evansella cellulosilytica (strain ATCC 21833 / DSM 2522 / FERM P-1141 / JCM 9156 / N-4) OX=649639 GN=celB PE=3 SV=1
P23549	2.60e-09	1	456	1	439	Endoglucanase OS=Bacillus subtilis OX=1423 GN=bglC PE=3 SV=1
O85465	9.59e-08	1	325	1	289	Endoglucanase 5A OS=Salipaludibacillus agaradhaerens OX=76935 GN=cel5A PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.000244	0.998994	0.000209	0.000189	0.000175	0.000161

TMHMM  Annotations     

start	end
5	27