CAZyme Information: MGYG000003005_01166

Basic Information

• Lineage: Bacteria; Firmicutes_A; Clostridia; Oscillospirales; Ruminococcaceae; Ruminococcus
• CAZyme ID: MGYG000003005_01166
• CAZy Family: PL1
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
1248	MGYG000003005_78|CGC1	132373.82	4.8297

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000003005	2620760	MAG	United States	North America

• Gene Location: Start: 2776; End: 6522 Strand: +

Full Sequence      

MKNMAMKKRIASIAAAAVVSVSCVAGTLGSANVILADNGSITAFAAESSVKFIQTVGYGE
AMYATWSSVTGASGYNVYVDGVQIDSMLIRQYSGYMRADAVGLKSGSHTMKVVPVINGKE
DSSKAAETKATAYAHDRSGFGWVNGTSSGAYNEDGTLKSNAVVIYVTDANKDSVKASIDS
TGKGASELTGVQNIILGYKKGKESRPLCVRFIGNITDPANMPSGDLMVDTVTAGLTIEGI
GNDTVFNGFGLVMKNSSNVEVRNIGFMNCNSKEGDDCGLQQKNDHVWVHNCDFFYGDAGS
DDDQVKGDGALDTKTSTYVTHSYNHFWDNGKCNLQGMKSESTENYITYHHNWYDHSDSRH
PRIRTCSVHIYNNYFDGNAKYGVGVTMGASAFVENNYFRNCKYPMLISKQGSDDITGGTF
SGEDGGVIKACGNSITGAKAYTTYKDNATDFDAYEVSSAKDTVPSSVKSKQGGTSYNNFD
TSSVMYKYTPDSPEAAKDKVMAQAGRVDGGDLKWTFDNSVDDESYAVNDGLKSALVNYKP
TVVAIGSGFKEDSSNPPTSSSTTTTTTTTTTSNTPVVTTVTTPNPPQPITGTDVIYASPK
GGGDGKTIDTPTDVLSAIKSVNAGGTIYLLKGTYSYTSTILIEEGNSGSAGKYKTISAYP
GANVKFDFTGQAVAGANRGFVLDGSYWHFYGFEIANAGDNGMLLSGDNNIIEMMVFNGNQ
DTGLQLSRYQTAYNTIAQWPSYNLIKNCTSKNNCDDATMENADGFAAKLTCGEGNVFDGC
MSYCNSDDGWDLYAKTETGPIGVVTIKNSIAFRNGFTESGKGYGNCDGNGFKLGGGGVGT
RHKVENCLAFENLNCGFTDNNNPEFGDMKNCTAYNNGVGGNGKSNYMVYRCSSSATFDGM
MSYTNVSKLSNTGAAGIKLSNDKFVGSMTNSIYYNSKYYFAKDKTTMTNGAKLGDIITPA
VSDFTTLDVPAMGTDFHTAWRNADGSINTKGFAETPSNGTYSSIGYHMSGGTSQQPSGST
TTTTTTSTTTSSSTTTTTTTTGNNPGPVSGGYVHNFTENGTASDYYTITGNLSTGKGTVE
YGGKTLTQCLKMESATNITFKAANAGKITLVFVEPAATIKIDGTKYTASGDGIITVDLEA
GDHAVTKADTANLFYMVYAESTTNPNPEISWGDANCDGQVDMSDAVLIMQSNSNPDKYGL
NGSDPTHITEQGLLNADVFENGASGVTNQDALSIQKWRLQLIPALPES

Enzyme Prediction     

No EC number prediction in MGYG000003005_01166.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
PL9	593	998	1.1e-123	0.976
PL1	230	400	2.8e-45	0.8217821782178217
CBM77	1052	1154	8e-42	0.9902912621359223

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
COG3866	PelB	2.72e-49	170	512	41	344	Pectate lyase [Carbohydrate transport and metabolism].
pfam18283	CBM77	9.77e-43	1050	1156	1	108	Carbohydrate binding module 77. This domain is the non-catalytic carbohydrate binding module 77 (CBM77) present in Ruminococcus flavefaciens. CBMs fulfil a critical targeting function in plant cell wall depolymerisation. In CBM77, a cluster of conserved basic residues (Lys1092, Lys1107 and Lys1162) confer calcium-independent recognition of homogalacturonan.
smart00656	Amb_all	1.59e-31	230	402	13	190	Amb_all domain.
pfam00544	Pec_lyase_C	5.03e-22	236	398	38	211	Pectate lyase. This enzyme forms a right handed beta helix structure. Pectate lyase is an enzyme involved in the maceration and soft rotting of plant tissue.
cd14256	Dockerin_I	1.02e-04	1172	1236	2	54	Type I dockerin repeat domain. Bacterial cohesin domains bind to a complementary protein domain named dockerin, and this interaction is required for the formation of the cellulosome, a cellulose-degrading complex. The cellulosome consists of scaffoldin, a noncatalytic scaffolding polypeptide, that comprises repeating cohesion modules and a single carbohydrate-binding module (CBM). Specific calcium-dependent interactions between cohesins and dockerins appear to be essential for cellulosome assembly. This subfamily represents type I dockerins, which are responsible for anchoring a variety of enzymatic domains to the complex.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
AOR96287.1	2.65e-258	49	1004	54	1084
ADU23076.1	1.61e-257	1	554	1	556
ABX41986.1	1.04e-256	40	1004	29	1154
QMW93302.1	1.29e-255	49	1004	54	1084
BBK78741.1	1.29e-255	49	1004	54	1084

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
5FU5_A	5.50e-44	1046	1159	2	114	Thecomplexity of the Ruminococcus flavefaciens cellulosome reflects an expansion in glycan recognition [Ruminococcus flavefaciens]
1RU4_A	4.95e-35	594	878	16	294	ChainA, Pectate lyase [Dickeya chrysanthemi]
5M2S_B	4.20e-25	1171	1247	28	103	R.flavefaciens' third ScaB cohesin in complex with a group 1 dockerin [Ruminococcus flavefaciens FD-1]
1AIR_A	7.39e-25	167	505	2	339	ChainA, PECTATE LYASE C [Dickeya chrysanthemi],1O88_A Chain A, Pectate Lyase C [Dickeya chrysanthemi],1O8D_A Chain A, PECTATE LYASE C [Dickeya chrysanthemi],1O8E_A Chain A, PECTATE LYASE C [Dickeya chrysanthemi],1O8F_A Chain A, PECTATE LYASE C [Dickeya chrysanthemi],1O8G_A Chain A, PECTATE LYASE C [Dickeya chrysanthemi],1O8H_A Chain A, PECTATE LYASE C [Dickeya chrysanthemi],1O8I_A Chain A, PECTATE LYASE C [Dickeya chrysanthemi],1O8J_A Chain A, PECTATE LYASE C [Dickeya chrysanthemi],1O8K_A Chain A, PECTATE LYASE C [Dickeya chrysanthemi],1O8L_A Chain A, PECTATE LYASE C [Dickeya chrysanthemi],1O8M_A Chain A, PECTATE LYASE C [Dickeya chrysanthemi],1PLU_A Chain A, Protein (pectate Lyase C) [Dickeya chrysanthemi],2PEC_A Chain A, PECTATE LYASE C [Dickeya chrysanthemi]
2EWE_A	1.81e-24	167	505	2	339	ChainA, Pectate lyase C [Dickeya chrysanthemi]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
P0C1A6	5.33e-35	594	925	41	369	Pectate lyase L OS=Dickeya chrysanthemi OX=556 GN=pelL PE=3 SV=1
P0C1A7	4.34e-34	594	878	41	319	Pectate lyase L OS=Dickeya dadantii (strain 3937) OX=198628 GN=pelL PE=1 SV=1
P22751	1.02e-31	595	876	391	639	Pectate disaccharide-lyase OS=Dickeya chrysanthemi OX=556 GN=pelX PE=1 SV=1
P0C1C1	3.15e-24	145	505	34	360	Pectate lyase 2 OS=Pectobacterium carotovorum OX=554 GN=pel2 PE=3 SV=1
P11073	3.20e-24	163	505	20	361	Pectate lyase C OS=Dickeya chrysanthemi OX=556 GN=pelC PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.001837	0.534030	0.462835	0.000761	0.000322	0.000206

TMHMM  Annotations     

start	end
13	35