CAZyme Information: MGYG000002922_00724

Basic Information

• Species: RC9 sp900541925
• Lineage: Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; UBA932; RC9; RC9 sp900541925
• CAZyme ID: MGYG000002922_00724
• CAZy Family: GH139
• CAZyme Description: Beta-galactosidase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
1582	MGYG000002922_5|CGC2	178044.04	5.5858

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000002922	2264361	MAG	United States	North America

• Gene Location: Start: 43231; End: 47979 Strand: -

Full Sequence      

MSTAKILCTAALTAVLCTMSPLTKADNHTKALISQYDVSWTTQSSGPDASMPCGGGELGL
NVWVENSEVMIYFSDGVWLDENNALLKGGRLRLRLCPDPFGGRDFRQTLRLWDGHMEISG
GDVTMTIWADVEKPVFHIDINSAHPTAIKASYESWRYEDRPIGDEKNATSWKWALPNDAK
FTSDIFECDESSLTFRHENGPVTIFDYTVEKEGLGAYKADMYNPIGNLVWGGTVNFGGMR
FSGTSFGEYIDTPFKAWNYEGGGATRYSIVFCGAAVQGNSPSGEDAAGLWRESLKKTKDS
VFDHADRAAALDWWHSYWSRSYIVTDPAHPGSEIWKIGRNYQLFRYMLGCNARGYWPTKF
NGGLFTFDSRFVQTPSPQHYSPDYRNWCGGTMTAQNQRLVYFPMLKSGDWDMMRPQFEFY
RRTLGNAELRSRVYWGHGGACFTEQLENYGLPNPAEYGTNRPEGMDPGMQDNRWLEYQWD
TVLEFCLMILETDRYCGADIAEYLPMIESCLDFFEQHYRYLARSRGERELDAQGKLVIFP
GSGCETYKIARNPSSTIAALQRVSESLSAYYLKKDLPDKAVRWQRFRSIIPEIPYREANG
RKYISPAEEWERVQNVETPQLYPVFPWRVIRPGDERHEYAVNTWWNDSDALKFRSHTGWK
QDAVWAACMGLTDEAERLILLKYADGPHRFPAFWGPGFDWTPDHNWGGSAAIALQEMILG
EDAQGNVTTLASWPSDWPVEYRLHGHDGRVHTNTPSGTTSVAGFFPLEGSGRIVLSFNPG
WKYFKGEAEGAHEPSFDDSAWESVNLPHTVELLPSEGSGNRNYQGPAWYRKTFTIPSEFG
DRHAVLHFEAVMGKQSVWVNGQKVQDNLGGYLPFEVDLTACGVKPGQNCTVALLADNSDD
RSYPPGKTQYTLDFTYHGGIYRDVWLLGIPEVHVTRASTAGKIAGGGVFIHYDAVSREKA
SMTVDTDLGNFGEKTRRISLRTRILSPDGTPVSEKTDRVSVPVGGKSIRQTFTLDRPRLW
SPESPTLYSVETQLFDKDKAIDGGITRTGVRSVRFDKDRGLVLNGEPYGQVIGVNRHQDF
AYVGNAVPNNVQWADARRLRDAGFKIIRTAHYPQDPSFMDACDELGLFVIVATPGWQFWN
NDPEFASRVHDNTRRIIRRDRNHPSVILWEPILNETMFPLDFSLEALRLTREEYPYAGAP
GAAADLNSKGVRESYDVAYGWPEDKGTVDKPLFTREFGEMVDDWYAHNAINRAARHWGEI
PMLMASSSLHSTLGQVMVPGGHYFGGAQWHPFDHQRGYHPDPYWGGDWDAFRQEKYAYEM
FRGALTKTEEPFIFFANEMGQFSPDDVTIYSNCDSVRVWGLDGKPVSVASDNGTFVFPGL
WNFWEARAESYSRRNWKGVCLFAEGFRDGRVVCTSSKMPSRRSTRLRLRLDPVYGPLPAD
GSSFVVVIAEVTDDNGNVRRLAKEDILFTVEGEGEIIGDASIGANPRAVEFGSAPVLIRS
TATPGRIRVNARVLHEGTHAPSPASLEFYSVEPPFESLGDASRSARVVGTDGRSTGGVHL
SSEESSRLLQEVERQQAEFGIR

Enzyme Prediction     

EC	3.2.1.-

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH139	13	745	8.9e-270	0.9433719433719434
GH2	774	1257	1e-91	0.5492021276595744

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
pfam18961	DUF5703_N	1.23e-100	40	323	2	287	Domain of unknown function (DUF5703). This is an N-terminal domain of unknown function mostly found in bacteria. It is possible that this domain might be a putative glycoside hydrolase. This family belongs to the Galactose Mutarotase-like superfamily.
COG3250	LacZ	2.26e-52	766	1179	4	410	Beta-galactosidase/beta-glucuronidase [Carbohydrate transport and metabolism].
PRK10150	PRK10150	1.17e-45	765	1175	3	417	beta-D-glucuronidase; Provisional
PRK10340	ebgA	1.97e-37	829	1182	113	456	cryptic beta-D-galactosidase subunit alpha; Reviewed
PRK09525	lacZ	3.30e-26	829	1169	124	457	beta-galactosidase.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QRQ56971.1	0.0	752	1580	25	881
ALJ45345.1	0.0	752	1580	25	881
QUT77868.1	0.0	760	1581	29	870
QUT23646.1	0.0	752	1580	25	881
QRN01891.1	0.0	752	1580	25	881

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
5UJ6_A	6.16e-59	772	1169	25	413	CrystalStructure of Bacteroides Uniformis beta-glucuronidase [Bacteroides uniformis str. 3978 T3 ii],5UJ6_B Crystal Structure of Bacteroides Uniformis beta-glucuronidase [Bacteroides uniformis str. 3978 T3 ii],6NZG_A Bacteroides uniformis beta-glucuronidase 2 covalently bound to cyclophellitol-6-carboxylate aziridine [Bacteroides uniformis],6NZG_B Bacteroides uniformis beta-glucuronidase 2 covalently bound to cyclophellitol-6-carboxylate aziridine [Bacteroides uniformis]
6D50_A	6.72e-59	772	1169	33	421	Bacteroidesuniforms beta-glucuronidase 2 bound to D-glucaro-1,5-lactone [Bacteroides uniformis str. 3978 T3 ii],6D50_B Bacteroides uniforms beta-glucuronidase 2 bound to D-glucaro-1,5-lactone [Bacteroides uniformis str. 3978 T3 ii]
5EUV_A	4.03e-58	772	1512	2	717	ChainA, Beta-D-galactosidase [Paracoccus sp. 32d],5EUV_B Chain B, Beta-D-galactosidase [Paracoccus sp. 32d],5LDR_B Chain B, Beta-D-galactosidase [Paracoccus sp. 32d]
5LDR_A	4.09e-58	772	1512	3	718	ChainA, Beta-D-galactosidase [Paracoccus sp. 32d]
6D8G_A	1.61e-57	772	1169	33	421	D341AD367A calcium binding mutant of Bacteroides uniformis beta-glucuronidase 2 [Bacteroides uniformis str. 3978 T3 ii],6D8G_B D341A D367A calcium binding mutant of Bacteroides uniformis beta-glucuronidase 2 [Bacteroides uniformis str. 3978 T3 ii]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
P77989	5.39e-86	771	1512	2	724	Beta-galactosidase OS=Thermoanaerobacter pseudethanolicus (strain ATCC 33223 / 39E) OX=340099 GN=lacZ PE=3 SV=2
P26257	1.89e-49	772	1478	2	706	Beta-galactosidase OS=Thermoanaerobacterium thermosulfurigenes OX=33950 GN=lacZ PE=1 SV=1
T2KN75	2.69e-49	767	1512	21	782	Beta-glucuronidase OS=Formosa agariphila (strain DSM 15362 / KCTC 12365 / LMG 23005 / KMM 3901 / M-2Alg 35-1) OX=1347342 GN=BN863_22060 PE=1 SV=1
T2KPJ7	1.08e-46	760	1182	39	437	Putative beta-glucuronidase OS=Formosa agariphila (strain DSM 15362 / KCTC 12365 / LMG 23005 / KMM 3901 / M-2Alg 35-1) OX=1347342 GN=BN863_21970 PE=2 SV=1
T2KM09	5.41e-41	772	1504	46	799	Putative beta-glucuronidase OS=Formosa agariphila (strain DSM 15362 / KCTC 12365 / LMG 23005 / KMM 3901 / M-2Alg 35-1) OX=1347342 GN=BN863_22050 PE=2 SV=2

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.000215	0.999181	0.000168	0.000153	0.000136	0.000128

TMHMM  Annotations     

There is no transmembrane helices in MGYG000002922_00724.