CAZyme Information: MGYG000002890_00159

Basic Information

• Species: Streptococcus oralis
• Lineage: Bacteria; Firmicutes; Bacilli; Lactobacillales; Streptococcaceae; Streptococcus; Streptococcus oralis
• CAZyme ID: MGYG000002890_00159
• CAZy Family: GH101
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
2139		235452.1	5.421

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000002890	1541311	MAG	United States	North America

• Gene Location: Start: 11164; End: 17583 Strand: -

Full Sequence      

MDKRFFEKRCKFSIRKFTLGVASVMIGATFFAASPVLADQARVGSTDNLPSELADLDKKA
SDEGHDFDKEAAAQNPGSAETTEGPQTEEELLAQEKEKSEKPSNLPKELEDKLEKAEDNG
REVDKDQLAQDTGKLVPEDVAKTSNGELNYGATVKIKTPSGEGSGIVVAKDLVLTVSHNF
IKDSQDGNIRKVVDNDQGDGDIYSISYPGLPDVKFSKKDIIHWDREGYLKGFKNDLALVR
LRTVLENTPVEVTKKPVVKKIGDKLHVFGYPEGKLNPIVNTTVDFAEPYGEGVQGIGYQG
GKPGASGGGIFDTEGKLVGVHQNGVVGKRSGGILFSPAQLKWIQDHIQGISSVKPADLEE
KEKPAEEKPKEDKPAAAKPETPKTVTPEWQTVANKEQQGTVTIREEKGVRYNQLSSTAQN
DNGDKPALFEKQGLTVDANGNATVDLTFKDDSEKGKSRFGVFLKFKDTKNNVFVGYDQGG
WFWEYKTPGNSTWYQGGRVAAPVNGSVNHLTISLKSDGQLNATNNDVKLFDTVTLPAAVN
ENLKNEKKILLKAGTYSNDRTVVSVKTDNQEGVKADDTPAQKETGPAVDDSKVTYDTIQS
KVLKAVIDQAFPRVKEYTLNGHTLPGQVQQLKKILVNNREITPEVTYKKINETTAEYLMK
LRDEKNFINADMTVRLQVVDNQLHFDVTKIVNHNQVTPGQKIDDERKLLSSINFLGNSLV
SVSSDQTGAKFDGATMSNNTHISGDEHIAVTNPMKDLAKGYMYGFVSTDKLAAGVWSNSQ
NSYGGGSNDWTRLTAFKQTVGNTNYVGIQSSEWQWEKAYKGIVFPEYTKELPSAKVVITE
DANADNKVDWQDGAIAYRSIMNNPQGWEKVKDITAYRIAMNFGSQAQNPFLMTLDGIKKI
NLHTDGLGQGVLLKGYGSEGHDSGHLNYADIGKRIGGVEDFKTLIEKAKKYGAHLGIHVN
ASETYPESKYFNENILRKNPDGSYSYGWNWLDQGINIDAAYDLAHGRLARWEDLKKKLGE
GLDFIYVDVWGNGQSGDNGAWATHVLAKEINKQGWRFAIEWGHGGEYDSTFQHWAADLTY
GGYTNKGINSAITRFIRNHQKDSWVGDYRSYGGAANYPLLGGYSMKDFEGWQGRSDYNGY
VTNLFAHDVMTKYFQHFTVSKWENGTPVTMSDNGSTYKWTPEMKVELVDAAGNKVVVTRK
SNDVNSPQYRERTVTLNGRVIQDGSAYLTPWNWDANGKKLPTDKEKMYYFNTQAGATTWT
LPSDWANSKVYLYKLTDQGKTEEQELTVKDGKITLDLLANQPYVLYRSKQTNPEMSWSEG
MHIYDQGFNSGTLKHWTISGDASKAEIVKSQGANEMLRIQGNKSKVSLTQKLTGLKPNTK
YAVYVGVDNRSNAKASITVNTGEKEVTTYTNKSLALNYIKAYAHNNRRENATVDDTSYFQ
NMYAFFTTGSDVSNVTLTLSREAGDEATYFDEIRTFENNSSMYGDKHDTGKGTFKQDFEN
VAQGIFPFVVGGVEGVEDNRTHLSEKHDPYTQRGWNGKKVDDVIEGNWSLKTNGLVSRRN
LVYQTIPQNFRFEAGKTYRVTFEYEAGSDNTYAFVVGKGEFQSGRRGSQASNLEMHELPN
TWTDSKKAKKATFLVTGAETGDTWVGIYSTGNASNTRGDAGGNANFRGYNDFMMDNLHIE
EITLTGKMLTENALKNYLPTVAMTNYTKESMDALKEAVFNLSQADDDISVEEARAEIAKI
EALKNALVQKKTALVAEDFESLDAPAQPGEGLENAFDGNVSSLWHTSWNGGDVGKPATMV
LKEPTEITGLRYVPRASDSNGNLRDVKLVVTDESGKEHTFTVTDWPNNNKPKDIDFGKTI
KAKKIVLTGTKTYGDGGDKYQSAAELIFTRPQVAETPLDMSGYEAALAKAQKLTDKDNQE
EVASVQASMKYATDNHLLTERMVAYFADYLNQLKDSATKPDAPTSSKGEEQPPVLDVPEF
KGGVNATEAAVHEVPEFKGGVNAVEAAVHEVPEFKGGVNAAEAAVHDLAEFKGGVNAVLA
AANEVPEYRGGVNAVEALVNEKPAYTGVLATAGDQAAPTVEKPEYPLTPSPVADTKTPGA
KDEEKLPATGEHSSEVALFLASVSIALSAAVLTTKRKED

Enzyme Prediction     

No EC number prediction in MGYG000002890_00159.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH101	600	1308	0	0.9985855728429985

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
pfam12905	Glyco_hydro_101	2.90e-161	851	1141	1	273	Endo-alpha-N-acetylgalactosaminidase. Virulence of pathogenic organisms such as the Gram-positive Streptococcus pneumoniae is largely determined by the ability to degrade host glycoproteins and to metabolize the resultant carbohydrates. This family is the enzymatic region, EC:3.2.1.97, of the cell surface proteins that specifically cleave Gal-beta-1,3-GalNAc-alpha-Ser/Thr (T-antigen, galacto-N-biose), the core 1 type O-linked glycan common to mucin glycoproteins. This reaction is exemplified by the S. pneumoniae protein Endo-alpha-N-acetylgalactosaminidase, where Asp764 is the catalytic nucleophile-base and Glu796 the catalytic proton donor.
cd14244	GH_101_like	4.86e-132	864	1164	1	298	Endo-a-N-acetylgalactosaminidase and related glcyosyl hydrolases. This family contains the enzymatically active domain of cell surface proteins that specifically cleave Gal-beta-1,3-GalNAc-alpha-Ser/Thr (T-antigen, galacto-N-biose), the core 1 type O-linked glycan common to mucin glycoproteins (EC:3.2.1.97). It has been classified as glycosyl hydrolase family 101 in the Cazy resource. Virulence of pathogenic organisms such as the Gram-positive Streptococcus pneumoniae and other commensal human bacteria is largely determined by their ability to degrade host glycoproteins and to metabolize the resultant carbohydrates.
pfam18080	Gal_mutarotas_3	5.59e-112	598	850	1	243	Galactose mutarotase-like fold domain. This domain is found in endo-alpha-N-acetylgalactosaminidase present in Streptococcus pneumoniae. Endo-alpha-N-acetylgalactosaminidase is a cell surface-anchored glycoside hydrolase involved in the breakdown of mucin type O-linked glycans. The domain, known as domain 2, exhibits strong structural similarlity to the galactose mutarotase-like fold but lacks the active site residues. Domains, found in a number of glycoside hydrolases, structurally similar to domain 2 confer stability to the multidomain architectures.
pfam17974	GalBD_like	2.90e-104	1471	1668	1	190	Galactose-binding domain-like. Proteins containing a galactose-binding domain-like fold can be found in several different protein families, in both eukaryotes and prokaryotes. The common function of these domains is to bind to specific ligands, such as cell-surface-attached carbohydrate substrates for galactose oxidase and sialidase, phospholipids on the outer side of the mammalian cell membrane for coagulation factor Va, membrane-anchored ephrin for the Eph family of receptor tyrosine kinases, and a complex of broken single-stranded DNA and DNA polymerase beta for XRCC1. The structure of the galactose-binding domain-like members consists of a beta-sandwich, in which the strands making up the sheets exhibit a jellyroll fold.
pfam17995	GH101_N	1.46e-92	389	570	1	180	Endo-alpha-N-acetylgalactosaminidase N-terminal. This is the N-terminal domain found in Streptococcus pneumoniae endo-alpha-N-acetylgalactosaminidase (EC:3.2.1.97), a cell surface-anchored glycoside hydrolase from family GH101 involved in the breakdown of mucin type O-linked glycans. This is a twisted beta-sandwich domain composed of two sheets of six and seven antiparallel beta-strands. The domain appears to be missing the extended metal and carbohydrate-binding loops.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
VEF78371.1	0.0	1	2139	1	2141
AQA07482.1	0.0	1	2139	1	2121
QLL96372.1	0.0	1	2139	1	2121
QLL98145.1	0.0	1	2139	1	2122
QQK99919.1	0.0	1	2139	1	2140

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
6QFK_A	0.0	591	1682	5	1116	EngBFDARPin Fusion 4b G10 [Bifidobacterium longum]
5A59_A	0.0	581	1690	2	1111	Thestructure of GH101 E796Q mutant from Streptococcus pneumoniae TIGR4 in complex with T-antigen [Streptococcus pneumoniae TIGR4],5A5A_A The structure of GH101 E796Q mutant from Streptococcus pneumoniae TIGR4 in complex with PNP-T-antigen [Streptococcus pneumoniae TIGR4]
6QEP_A	0.0	591	1682	5	1116	EngBFDARPin Fusion 4b H14 [Bifidobacterium longum]
6QEV_B	0.0	591	1682	5	1116	EngBFDARPin Fusion 4b B6 [Bifidobacterium longum]
2ZXQ_A	0.0	593	1889	22	1372	Crystalstructure of endo-alpha-N-acetylgalactosaminidase from Bifidobacterium longum (EngBF) [Bifidobacterium longum]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
Q2MGH6	0.0	360	1969	96	1705	Endo-alpha-N-acetylgalactosaminidase OS=Streptococcus pneumoniae serotype 4 (strain ATCC BAA-334 / TIGR4) OX=170187 GN=SP_0368 PE=1 SV=1
Q8DR60	0.0	360	1969	96	1705	Endo-alpha-N-acetylgalactosaminidase OS=Streptococcus pneumoniae (strain ATCC BAA-255 / R6) OX=171101 GN=spr0328 PE=1 SV=1
A9WNA0	4.79e-118	597	1682	51	1037	Putative endo-alpha-N-acetylgalactosaminidase OS=Renibacterium salmoninarum (strain ATCC 33209 / DSM 20767 / JCM 11484 / NBRC 15589 / NCIMB 2235) OX=288705 GN=RSal33209_1326 PE=3 SV=2
P29767	7.23e-11	1758	1901	55	194	Sialidase OS=Clostridium septicum OX=1504 PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.000378	0.998896	0.000183	0.000198	0.000183	0.000151

TMHMM  Annotations     

start	end
12	34