CAZyme Information: MGYG000002883_00897

Basic Information

• Species: UMGS1477 sp900553845
• Lineage: Bacteria; Cyanobacteria; Vampirovibrionia; Gastranaerophilales; Gastranaerophilaceae; UMGS1477; UMGS1477 sp900553845
• CAZyme ID: MGYG000002883_00897
• CAZy Family: GT51
• CAZyme Description: Monofunctional biosynthetic peptidoglycan transglycosylase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
701		77380.19	9.3214

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000002883	1791873	MAG	Ireland	Europe

• Gene Location: Start: 4412; End: 6517 Strand: -

Full Sequence      

MRRKKTVNNNGFINLIKNLITISLAVLLAFYAGFKFYIASLPPIENLESFTPNIVTKIYS
QDGDIIKTFTAYKFEHISVEKVPKHLIEAIIATEDKNFYSHNGYDVFGLIRSTFANLKAG
SFVQGASTITQQLARILFLNNDKTFDRKIKEFIVSAQIEKTLSKDQILEMYLNNVYLGSG
AYGVEGASQIYFNKSVKDLNLAQCALIAGLPQAPSIYSPFNNPELAKKRQGHVLKRMLKT
GYITREEYNKAAETELVLNKNPHIYSYNKAPYFIDLVMNELNSLGFDERDVSSGGYKITT
TLNYKAQKAADEAVRKYLKAYGLTKEKQQAACYGVSPLTGEILVYVGGKNYEKSQYDRIQ
NAVRPPGSAFKPFVYAAALQKGWKITDKLADTPVTIGDWSPRNYGSKYRGTMPLYKGLTY
SSNVMAAKLIKDTGVRPVVSLVRSLGISTPIENDLTIALGSNGVKLSEMVIAYGAFANGG
YKVKPYSVLRVETSRGKVLYEAPKARIMKAISTETASGITQMLKTVITEGTGRAANINKP
AAGKSGTTDDYKDAWFIGYTPDVVLGVWVGNDDNSKMSIALTGGSVPALIWKDAMKIATE
NFGNVDFSYEPIDIIKEKPQIQNNYIPSEEIIEKEDNQNTDGNTPENQAVTTDETIIDKT
SSSTKPVNTEVGNKPAPTQTGHQPETPSHVNMPEPKPSEGE

Enzyme Prediction     

No EC number prediction in MGYG000002883_00897.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GT51	62	237	6.3e-68	0.9887005649717514

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
COG0744	MrcB	0.0	12	666	11	661	Membrane carboxypeptidase (penicillin-binding protein) [Cell wall/membrane/envelope biogenesis].
COG5009	MrcA	0.0	15	608	4	728	Membrane carboxypeptidase/penicillin-binding protein [Cell wall/membrane/envelope biogenesis].
TIGR02074	PBP_1a_fam	0.0	75	601	3	531	penicillin-binding protein, 1A family. Bacterial that synthesize a cell wall of peptidoglycan (murein) generally have several transglycosylases and transpeptidases for the task. This family consists of bifunctional transglycosylase/transpeptidase penicillin-binding proteins (PBP). In the Proteobacteria, this family includes PBP 1A but not the paralogous PBP 1B (TIGR02071). This family also includes related proteins, often designated PBP 1A, from other bacterial lineages. [Cell envelope, Biosynthesis and degradation of murein sacculus and peptidoglycan]
COG4953	PbpC	8.31e-107	19	598	4	561	Membrane carboxypeptidase/penicillin-binding protein PbpC [Cell wall/membrane/envelope biogenesis].
TIGR02073	PBP_1c	3.71e-104	56	606	12	551	penicillin-binding protein 1C. This subfamily of the penicillin binding proteins includes the member from E. coli designated penicillin-binding protein 1C. Members have both transglycosylase and transpeptidase domains and are involved in forming cross-links in the late stages of peptidoglycan biosynthesis. All members of this subfamily are presumed to have the same basic function. [Cell envelope, Biosynthesis and degradation of murein sacculus and peptidoglycan]

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
AOR38680.1	1.64e-277	9	615	28	634
ACI19842.1	2.59e-151	24	628	28	641
AEE14353.1	6.07e-149	12	598	8	592
ACK42407.1	7.06e-149	17	609	18	620
CCK81656.1	2.48e-148	17	596	4	596

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
4OON_A	1.62e-99	44	601	9	725	Crystalstructure of PBP1a in complex with compound 17 ((4Z,8S,11E,14S)-5-(2-amino-1,3-thiazol-4-yl)-14-(5,6-dihydroxy-1,3-dioxo-1,3-dihydro-2H-isoindol-2-yl)-8-formyl-2-methyl-6-oxo-3,10-dioxa-4,7,11-triazatetradeca-4,11-diene-2,12,14-tricarboxylic acid) [Pseudomonas aeruginosa PAO1]
3UDF_A	5.27e-92	57	595	22	712	ChainA, Penicillin-binding protein 1a [Acinetobacter baumannii],3UDF_B Chain B, Penicillin-binding protein 1a [Acinetobacter baumannii],3UDI_A Chain A, Penicillin-binding protein 1a [Acinetobacter baumannii],3UDI_B Chain B, Penicillin-binding protein 1a [Acinetobacter baumannii],3UDX_A Chain A, Penicillin-binding protein 1a [Acinetobacter baumannii],3UDX_B Chain B, Penicillin-binding protein 1a [Acinetobacter baumannii],3UE0_A Chain A, Penicillin-binding protein 1a [Acinetobacter baumannii],3UE0_B Chain B, Penicillin-binding protein 1a [Acinetobacter baumannii],3UE1_A Chain A, Penicillin-binding protein 1a [Acinetobacter baumannii],3UE1_B Chain B, Penicillin-binding protein 1a [Acinetobacter baumannii]
3ZG8_B	5.35e-73	157	585	2	446	CrystalStructure of Penicillin Binding Protein 4 from Listeria monocytogenes in the Ampicillin bound form [Listeria monocytogenes],3ZG9_B Crystal Structure of Penicillin-Binding Protein 4 from Listeria monocytogenes in the Cefuroxime bound form [Listeria monocytogenes],3ZGA_B Crystal Structure of Penicillin-Binding Protein 4 from Listeria monocytogenes in the Carbenicillin bound form [Listeria monocytogenes]
5U2G_A	6.66e-72	39	502	5	566	2.6Angstrom Resolution Crystal Structure of Penicillin-Binding Protein 1A from Haemophilus influenzae [Haemophilus influenzae Rd KW20],5U2G_B 2.6 Angstrom Resolution Crystal Structure of Penicillin-Binding Protein 1A from Haemophilus influenzae [Haemophilus influenzae Rd KW20]
5FGZ_A	1.02e-69	37	586	122	683	E.coli PBP1b in complex with FPI-1465 [Escherichia coli K-12],5HL9_A E. coli PBP1b in complex with acyl-ampicillin and moenomycin [Escherichia coli K-12],5HLA_A E. coli PBP1b in complex with acyl-cephalexin and moenomycin [Escherichia coli K-12],5HLB_A E. coli PBP1b in complex with acyl-aztreonam and moenomycin [Escherichia coli K-12],5HLD_A E. coli PBP1b in complex with acyl-CENTA and moenomycin [Escherichia coli K-12],6YN0_A Structure of E. coli PBP1b with a FtsN peptide activating transglycosylase activity [Escherichia coli K-12],7LQ6_A Chain A, Penicillin-binding protein 1B [Escherichia coli K-12]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
O66874	1.23e-116	17	615	2	684	Penicillin-binding protein 1A OS=Aquifex aeolicus (strain VF5) OX=224324 GN=mrcA PE=1 SV=1
Q07806	7.00e-103	20	601	12	752	Penicillin-binding protein 1A OS=Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) OX=208964 GN=mrcA PE=1 SV=2
O87579	1.64e-101	41	598	35	705	Penicillin-binding protein 1A OS=Neisseria lactamica OX=486 GN=mrcA PE=3 SV=1
P38050	3.40e-101	56	600	49	599	Penicillin-binding protein 1F OS=Bacillus subtilis (strain 168) OX=224308 GN=pbpF PE=2 SV=2
Q9ZCE9	9.57e-101	37	633	30	751	Penicillin-binding protein 1A OS=Rickettsia prowazekii (strain Madrid E) OX=272947 GN=mrcA PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000029	0.000025	0.000000	0.000000	0.000000	0.000002

TMHMM  Annotations     

start	end
12	34