CAZyme Information: MGYG000002789_01759

Basic Information

• Species: Lapidilactobacillus dextrinicus
• Lineage: Bacteria; Firmicutes; Bacilli; Lactobacillales; Lactobacillaceae; Lapidilactobacillus; Lapidilactobacillus dextrinicus
• CAZyme ID: MGYG000002789_01759
• CAZy Family: GH127
• CAZyme Description: Non-reducing end beta-L-arabinofuranosidase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
307	MGYG000002789_17|CGC1	35014.74	5.9748

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000002789	1826765	MAG	United States	North America

• Gene Location: Start: 4948; End: 5871 Strand: +

Full Sequence      

MVFFAKQMLETHMDGQYGDIIEKELFNGALSGIALDGKHYFYVNPLEADPKASRGNQGKN
HINTRRSSWFACACCPSNITRLIDSVDKYLYEVKDQTILSDQFIANNTSFENGIEIKLDS
NYPWDGNIKYTISNPNKVQFKFGIRIPGWTLNSYEVNIAGKPVKTNLIDQILYLPIEDKA
VEISVQFNMAAHLINTSTLVKDNIGKVAVQRGPIVYCAEESDNTSPLWLYQLDSKPAFKY
EFKNDLLDGVGVLETRDVKKIEENGPNDGLYTFDKEKIVKKSHLKLIPYYAWANREEGQM
TVWFHQS

Enzyme Prediction     

No EC number prediction in MGYG000002789_01759.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH127	1	217	5.2e-69	0.41030534351145037

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
COG3533	COG3533	1.74e-61	1	303	299	584	Uncharacterized conserved protein, DUF1680 family [Function unknown].
pfam07944	Glyco_hydro_127	6.52e-59	1	217	297	503	Beta-L-arabinofuranosidase, GH127. One member of this family, from Bidobacterium longicum, UniProtKB:E8MGH8, has been characterized as an unusual beta-L-arabinofuranosidase enzyme, EC:3.2.1.185. It rleases l-arabinose from the l-arabinofuranose (Araf)-beta1,2-Araf disaccharide and also transglycosylates 1-alkanols with retention of the anomeric configuration. Terminal beta-l-arabinofuranosyl residues have been found in arabinogalactan proteins from a mumber of different plantt species. beta-l-Arabinofuranosyl linkages with 1-4 arabinofuranosides are also found in the sugar chains of extensin and solanaceous lectins, hydroxyproline (Hyp)2-rich glycoproteins that are widely observed in plant cell wall fractions. The critical residue for catalytic activity is Glu-338, in a ET/SCAS sequence context.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QGV24837.1	9.52e-138	1	303	351	650
ALU37778.1	5.66e-114	1	307	350	657
AGY77639.1	5.66e-114	1	307	350	657
ADK14420.1	1.59e-113	1	307	350	657
QGV24955.1	3.94e-112	1	306	357	662

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
3WRE_A	8.75e-82	1	302	345	654	Thecrystal structure of native HypBA1 from Bifidobacterium longum JCM 1217 [Bifidobacterium longum subsp. longum JCM 1217],3WRG_A The complex structure of HypBA1 with L-arabinose [Bifidobacterium longum subsp. longum JCM 1217]
3WKW_A	1.10e-81	1	302	345	654	Crystalstructure of GH127 beta-L-arabinofuranosidase HypBA1 from Bifidobacterium longum ligand free form [Bifidobacterium longum subsp. longum JCM 1217],3WKX_A Crystal structure of GH127 beta-L-arabinofuranosidase HypBA1 from Bifidobacterium longum arabinose complex form [Bifidobacterium longum subsp. longum JCM 1217],7BZL_A Chain A, Non-reducing end beta-L-arabinofuranosidase [Bifidobacterium longum subsp. longum JCM 1217],7DIF_A Chain A, Non-reducing end beta-L-arabinofuranosidase [Bifidobacterium longum subsp. longum JCM 1217],7EXV_A Chain A, Non-reducing end beta-L-arabinofuranosidase [Bifidobacterium longum subsp. longum JCM 1217],7EXW_A Chain A, Non-reducing end beta-L-arabinofuranosidase [Bifidobacterium longum subsp. longum JCM 1217]
7EXU_A	1.10e-81	1	302	345	654	ChainA, Non-reducing end beta-L-arabinofuranosidase [Bifidobacterium longum subsp. longum JCM 1217]
3WRF_A	1.52e-78	4	302	348	654	Thecrystal structure of native HypBA1 from Bifidobacterium longum JCM 1217 [Bifidobacterium longum subsp. longum JCM 1217]
4QJY_A	8.68e-68	1	307	340	648	Crystalstructure of native Ara127N, a GH127 beta-L-arabinofuranosidase from Geobacillus Stearothermophilus T6 [Geobacillus stearothermophilus],4QJY_B Crystal structure of native Ara127N, a GH127 beta-L-arabinofuranosidase from Geobacillus Stearothermophilus T6 [Geobacillus stearothermophilus]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
E8MGH8	4.79e-81	1	302	345	654	Non-reducing end beta-L-arabinofuranosidase OS=Bifidobacterium longum subsp. longum (strain ATCC 15707 / DSM 20219 / JCM 1217 / NCTC 11818 / E194b) OX=565042 GN=hypBA1 PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.999317	0.000670	0.000037	0.000004	0.000001	0.000010

TMHMM  Annotations     

There is no transmembrane helices in MGYG000002789_01759.