CAZyme Information: MGYG000002693_02552

Basic Information

• Species: Anaerobutyricum sp002161065
• Lineage: Bacteria; Firmicutes_A; Clostridia; Lachnospirales; Lachnospiraceae; Anaerobutyricum; Anaerobutyricum sp002161065
• CAZyme ID: MGYG000002693_02552
• CAZy Family: GH116
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
663		76303.67	4.5748

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000002693	2842397	MAG	Canada	North America

• Gene Location: Start: 69; End: 2060 Strand: +

Full Sequence      

MKNVADCPTKVSLVGTLPNASGFEGYDVIENLKLADSVKNEYREFDDVKGLYYSPEHLKE
DHLRYGNMAILTSGSNVTYKTQWFDGEWVDGIQDFWDDFTSDGLLEKETVSDSVGCEFAQ
FHNFSFLKRREKIGSIGAWEELQPGEERTFEFVITWYFPNRVKAWIEFDEDYEKFQRGEY
GTVRNYYATKFTDAWDVAKYVYHNKERLESDSRKFADAMFHKTTLPYYVIDALTANITNL
RSNLCFRLEDGTFAGFEGIRDYIGCGYGSVPHVWNYAQTVAFLFPDLEKTMRNVEFLRET
DETGCMSTRMFSVFDQERYAMVPACDGELGSVVRVYRDFKNLGDVEFLKTIWPKVVLAME
YALKQWDLDGDDVLDGQQNTTYDIEFYGPNPMTDSIFLAALKCCEEMAEIVGDEEHHQLY
ADAYEKGAARADQLMFDGEYYIQVQKEIDKYKYQFGKGCLSDQLLGQFLAYMAGIGEILP
KEHVKSAMESVFKYNYKTDFYHTDSVHRAYAINEEHGMVVATWPKGGRPKFPLSYAGEVW
TGVEYEVAVNLIYSGCVEEGLTVVKSIRDRYDGYKRNPFSEIESGHHYCRAMASWGVLNA
LLGLQSDMYRGTLSFHPAIEGEMSSFFICGKAWGIYSQKEENGKMCKHIDILYGTLDDIH
VQE

Enzyme Prediction     

No EC number prediction in MGYG000002693_02552.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH116	271	597	1.2e-69	0.9449035812672176

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
pfam04685	DUF608	5.34e-49	326	597	99	362	Glycosyl-hydrolase family 116, catalytic region. This represents a family of archaeal, bacterial and eukaryotic glycosyl hydrolases, that belong to superfamily GH116. The primary catabolic pathway for glucosylceramide is catalysis by the lysosomal enzyme glucocerebrosidase. In higher eukaryotes, glucosylceramide is the precursor of glycosphingolipids, a complex group of ubiquitous membrane lipids. Mutations in the human protein cause motor-neurone defects in hereditary spastic paraplegia. The catalytic nucleophile, identified in UniProtKB:Q97YG8_SULSO, is a glutamine-335, with the likely acid/base at Asp-442 and the aspartates at Asp-406 and Asp-458 residues also playing a role in the catalysis of glucosides and xylosides that are beta-bound to hydrophobic groups. The family is defined as GH116, which presently includes enzymes with beta-glucosidase, EC:3.2.1.21, beta-xylosidase, EC:3.2.1.37, and glucocerebrosidase EC:3.2.1.45 activity.
COG4354	COG4354	3.79e-43	49	603	175	719	Uncharacterized protein, contains GBA2_N and DUF608 domains [Function unknown].
pfam12215	Glyco_hydr_116N	8.19e-30	2	208	129	309	beta-glucosidase 2, glycosyl-hydrolase family 116 N-term. This domain is found in bacteria, archaea and eukaryotes. This domain is typically between 320 to 354 amino acids in length. This domain is found associated with pfam04685. It is found just after the extreme N-terminus. The N-terminal is thought to be the luminal domain while the C terminal is the cytosolic domain. The catalytic domain of GBA-2 is unknown. The primary catabolic pathway for glucosylceramide is catalysis by the lysosomal enzyme glucocerebrosidase. In higher eukaryotes, glucosylceramide is the precursor of glycosphingolipids, a complex group of ubiquitous membrane lipids. Mutations in the human protein cause motor-neurone defects in hereditary spastic paraplegia. The catalytic nucleophile, identified in UniProtKB:Q97YG8_SULSO, is a glutamine-335 in the downstream family pfam04685.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QNM02725.1	0.0	1	663	159	821
QHW30807.1	8.84e-228	1	661	161	811
AZN42723.1	1.10e-227	1	662	170	820
AUA17208.1	4.89e-211	3	656	159	813
ALS26632.1	8.08e-211	11	662	167	810

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
5FJS_A	7.15e-22	11	597	190	780	Bacterialbeta-glucosidase reveals the structural and functional basis of genetic defects in human glucocerebrosidase 2 (GBA2) [Thermoanaerobacterium xylanolyticum LX-11],5FJS_B Bacterial beta-glucosidase reveals the structural and functional basis of genetic defects in human glucocerebrosidase 2 (GBA2) [Thermoanaerobacterium xylanolyticum LX-11],5O0S_A Crystal structure of txGH116 (beta-glucosidase from Thermoanaerobacterium xylolyticum) in complex with unreacted beta Cyclophellitol Cyclosulfate probe ME711 [Thermoanaerobacterium xylanolyticum LX-11]
5NPF_A	7.26e-22	11	597	193	783	Crystalstructure of txGH116 (beta-glucosidase from Thermoanaerobacterium xylolyticum) in complex with beta Cyclophellitol Cyclosulfate probe ME594 [Thermoanaerobacterium xylanolyticum]
5BVU_A	7.27e-22	11	597	194	784	Crystalstructure of Thermoanaerobacterium xylolyticum GH116 beta-glucosidase [Thermoanaerobacterium xylanolyticum LX-11],5BX2_A Crystal structure of Thermoanaerobacterium xylanolyticum GH116 beta-glucosidase with 2-deoxy-2-fluoroglucoside [Thermoanaerobacterium xylanolyticum LX-11],5BX3_A Crystal structure of Thermoanaerobacterium xylanolyticum GH116 beta-glucosidase with deoxynojirimycin [Thermoanaerobacterium xylanolyticum LX-11],5BX4_A Crystal structure of Thermoanaerobacterium xylanolyticum GH116 beta-glucosidase with Glucoimidazole [Thermoanaerobacterium xylanolyticum LX-11],5BX5_A Crystal structure of Thermoanaerobacterium xylanolyticum GH116 beta-glucosidase with glucose [Thermoanaerobacterium xylanolyticum LX-11],5NCX_A Crystal structure of Thermoanaerobacterium xylolyticum GH116 beta-glucosidase with an covalent inhibitor [Thermoanaerobacterium xylanolyticum LX-11],5OST_A Beta-glucosidase from Thermoanaerobacterium xylolyticum GH116 in complex with Gluco-1H-imidazole [Thermoanaerobacterium xylanolyticum LX-11]
7DKW_A	7.27e-22	11	597	194	784	ChainA, beta-glucosidase [Thermoanaerobacterium xylanolyticum LX-11],7DKW_B Chain B, beta-glucosidase [Thermoanaerobacterium xylanolyticum LX-11],7DKX_A Chain A, beta-glucosidase [Thermoanaerobacterium xylanolyticum LX-11],7DKY_A Chain A, beta-glucosidase [Thermoanaerobacterium xylanolyticum LX-11]
7W2V_A	7.65e-22	11	597	237	827	ChainA, Glucosylceramidase [Thermoanaerobacterium xylanolyticum LX-11]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
Q9HCG7	1.18e-18	69	560	339	841	Non-lysosomal glucosylceramidase OS=Homo sapiens OX=9606 GN=GBA2 PE=1 SV=2
Q5M868	4.27e-17	72	630	333	907	Non-lysosomal glucosylceramidase OS=Rattus norvegicus OX=10116 GN=Gba2 PE=2 SV=2
Q69ZF3	9.83e-17	326	560	610	832	Non-lysosomal glucosylceramidase OS=Mus musculus OX=10090 GN=Gba2 PE=1 SV=2
Q7KT91	3.40e-09	345	559	685	890	Non-lysosomal glucosylceramidase OS=Drosophila melanogaster OX=7227 GN=CG33090 PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000054	0.000000	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

There is no transmembrane helices in MGYG000002693_02552.