CAZyme Information: MGYG000002668_01640

Basic Information

• Species: Prevotella sp900552965
• Lineage: Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Bacteroidaceae; Prevotella; Prevotella sp900552965
• CAZyme ID: MGYG000002668_01640
• CAZy Family: GH133
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
488		56621.63	5.8414

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000002668	2820424	MAG	United Republic of Tanzania	Africa

• Gene Location: Start: 2789; End: 4255 Strand: -

Full Sequence      

MHENTNIIRDYQEVDGGIKTHLYEGYPDLYMQFSRKCEFIFRPDWYRGLLYDKELERGYE
GVEDLYVPGYFEVSAKKGDQIIFAAGLEEVKPNTLKKVFDQEVKIRTPRDNFYHTLVNSA
HQFHMRVSDKERYLLAGYPWFKVRARDMFISLPGLTIAIQENDYFKLVMDTAKKAIYEFM
SGRPLTGLIREIEQPDVLLWAIWALQQYAHEVGNAITYQEYGKFLEDMLRFMESGKHPNL
ELKENGLMHIEGREEPMTWMNSTVNGKPVVPRTGYVVEINALWYNALKFCAEMAISSGKP
EMAAELDARADIAKDSFQKIFVNPNGYLFDYVEEGITDWSVRPNMIFPVAFDYSPLEPEQ
KKSVLDFCTRELLTPKGLRTLSPKSGGYNPIYAGAQTQRDYAYHSGTAWPWLGGFYMEAC
IKVYKRTRLSFVQRQMLGYEDEMQYHVVGSISELFDGNPPYHGRGAGSFAMNVAEILRTW
RLIDQYKV

Enzyme Prediction     

No EC number prediction in MGYG000002668_01640.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH133	129	478	8.1e-97	0.978494623655914

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
pfam06202	GDE_C	1.70e-81	134	478	21	374	Amylo-alpha-1,6-glucosidase. This family includes human glycogen branching enzyme AGL. This enzyme contains a number of distinct catalytic activities. It has been shown for the yeast homolog GDB1 that mutations in this region disrupt the enzymes Amylo-alpha-1,6-glucosidase (EC:3.2.1.33).
TIGR01561	gde_arch	2.33e-53	11	419	148	573	glycogen debranching enzyme, archaeal type, putative. The seed for this model is composed of two uncharacterized archaeal proteins from Methanosarcina acetivorans and Sulfolobus solfataricus. Trusted cutoff is set so that essentially only archaeal members hit the model. The notable exceptions to archaeal membership are the Gram positive Clostridium perfringens which scores much better than some other archaea and the Cyanobacterium Nostoc sp. which scores just above the trusted cutoff. Noise cutoff is set to exclude the characterized eukaryotic glycogen debranching enzyme in S. cerevisiae. These cutoffs leave the prokaryotes Porphyromonas gingivalis and Deinococcus radiodurans below trusted but above noise. Multiple alignments including these last two species exhibit sequence divergence which may suggest a subtly different function for these prokaryotic proteins. [Energy metabolism, Biosynthesis and degradation of polysaccharides]
COG3408	GDB1	3.09e-44	9	478	160	601	Glycogen debranching enzyme (alpha-1,6-glucosidase) [Carbohydrate transport and metabolism].
pfam12439	GDE_N	1.02e-19	9	82	133	209	Glycogen debranching enzyme N terminal. This domain family is found in bacteria and archaea, and is typically between 218 and 229 amino acids in length. The family is found in association with pfam06202. Glycogen debranching enzyme catalyzes the debranching of amylopectin in glycogen. This is done by transferring three glucose subunits of glycogen from one parallel chain to another. This has the effect of enabling the glucose residues to become more accessible for glycolysis.
TIGR01531	glyc_debranch	8.17e-16	244	419	1183	1393	glycogen debranching enzymye. glycogen debranching enzyme possesses two different catalytic activities; oligo-1,4-->1,4-glucantransferase (EC 2.4.1.25) and amylo-1,6-glucosidase (EC 3.2.1.33). Site directed mutagenesis studies in S. cerevisiae indicate that the transferase and glucosidase activities are independent and located in different regions of the polypeptide chain. Proteins in this model belong to the larger alpha-amylase family. The model covers eukaryotic proteins with a seed composed of human, nematode and yeast sequences. Yeast seed sequence is well characterized. The model is quite rigorous; either query sequence yields large bit score or it fails to hit the model altogether. There doesn't appear to be any middle ground. [Energy metabolism, Biosynthesis and degradation of polysaccharides]

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QNT65228.1	2.61e-247	2	487	161	646
ALO49804.1	7.45e-247	2	486	151	635
VEH15920.1	5.47e-246	2	486	161	645
QVJ80484.1	7.53e-245	2	486	161	645
QUB46521.1	3.20e-241	2	486	161	645

PDB Hits     

has no PDB hit.

Swiss-Prot Hits     

has no Swissprot hit.

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000044	0.000000	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

There is no transmembrane helices in MGYG000002668_01640.