CAZyme Information: MGYG000002609_00719

Basic Information

• Species: CAG-882 sp003486385
• Lineage: Bacteria; Firmicutes_A; Clostridia; Lachnospirales; Lachnospiraceae; CAG-882; CAG-882 sp003486385
• CAZyme ID: MGYG000002609_00719
• CAZy Family: GH2
• CAZyme Description: Beta-galactosidase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
1207		135106.73	4.6733

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000002609	3323522	MAG	China	Asia

• Gene Location: Start: 13254; End: 16877 Strand: +

Full Sequence      

MNRTDLWDDGWSFRKTALGVTYEEALAGEFTAVDIPHDWLIYDTHNLYESSTGWYRKSFT
LTKEQLGKKLIVRFGAVYMDSTVFVNGSRVCEWKYGYSTFDADITEYVREGQNEMVVRAV
YQEPNTRWYSGAGIIRDVYFTSLEQVHIVPDGTYIRVRKNEDGTWKVKCTVELENSGKDV
PCEDIKVGFDLKHGGSSVFTFGRISPQGGGMFAAGSRKNAELVLEVDKPLIWDIDEPNLY
ECSVTVSVGDDTVHEENQKIGFCTKEYSPEKGFILNGRKVRINGVCEHHDNGCLGAAFNV
NAFRRKLDKLRKMGVNAIRTSHNMPAKEVLELADELGFLVDCEAFDMWELPKTEFDYARF
FVDWAERDVASWIRRDRNHVSVIMWSIGNEIYDTHRDEHGQEITRRLVGYVKANDPEGNA
PATIGSNYMPWENTQKCADIVKLAGYNYAEKYYEEHHKAHPDWVIYGSETASTLQSRGIY
KFPYEQQILCDDDEQCSALGNCSTNWGAKNSEYCITMDRDAEFSMGQFLWTGFDYIGEPT
PYQTKNSYFGQVDTAGFEKDTYYIYQAEWTDYRKAPMVHIFPYWDFSEGEEIDVRIASNA
PQVELFVNGTSYGRYDIDHKHGKQLLAHYKVEYVPGTIEAVAYDENGSVIARDARRSFSD
AAELVVEPESTQLIADGRALAYIDISAVDKDGNPVDNANNRVNIKVTGAGRLLGMDNGDS
ADFEQYKTNSRRMFSGKLLAVIGAKTEPGEIRVEVSSVGMETKVVILNTVAADNEVSGVS
CMEDCSGVCGKAGKASRDSGTGNVLDTAESDKTDMEEEIPVRNVRLIVKGSRRLGPDNRQ
VEIEAEILPHNATYRDIIWKVTNDAGVDSNGAELSDVEIPGGSAGTDAENGCAVSQKEGF
RIRKVLKALGDGKHNVRCCVKNGRDKVSLISQVSFTAEGLGLAEFNPYEFIYGTLYTAAE
QTPSVGQDRGIVPKGGTYIAFEKVNFGKNGSDEITIPVYELDNKPFPIEIWKGIPGEEGS
ELMDKVIYDKPSIWATYQEKTYKLPKRFKGLSTVSFIADHDINIKGFSFKKYERAYDENL
AVDNDTLYGDTYTLGSDAITGIGNNVTLTYKNMDFSHGVHGITICGRSVLEKNTIHIRFS
NDEGSVNQIAEFVMSDNTASGNAAGSAAGDDGYVCRHFELDNVEGIYDVNLVFLPGCNFD
LKWFKFE

Enzyme Prediction     

EC	3.2.1.23

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH2	8	601	1e-99	0.5877659574468085

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
COG3250	LacZ	1.13e-60	3	585	12	614	Beta-galactosidase/beta-glucuronidase [Carbohydrate transport and metabolism].
pfam18565	Glyco_hydro2_C5	1.43e-36	664	765	1	102	Glycoside hydrolase family 2 C-terminal domain 5. Domain 5 is found in dimeric beta-D-galactosidase from Paracoccus sp. 32d, which contributes to stabilization of the functional dimer. It is suggested that the location of this domain 5, may be one of the factors responsible for the creation of a functional dimer and cold-adaptation of this enzyme.
PRK10340	ebgA	9.50e-36	49	416	107	468	cryptic beta-D-galactosidase subunit alpha; Reviewed
PRK10150	PRK10150	4.00e-30	54	441	68	464	beta-D-glucuronidase; Provisional
PRK09525	lacZ	1.75e-25	49	423	118	497	beta-galactosidase.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QOS77051.1	0.0	1	1207	1	1168
QLG36749.1	0.0	1	1207	1	1158
APO44079.1	0.0	1	1207	1	1156
SQI53931.1	0.0	1	1206	1	1140
QZN78011.1	0.0	1	1207	1	1161

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
7CWD_A	3.19e-163	7	773	6	806	ChainA, beta-glalactosidase [Niallia circulans],7CWI_A Chain A, beta-galactosidase [Niallia circulans]
4YPJ_A	4.11e-162	7	762	12	800	ChainA, Beta galactosidase [Niallia circulans],4YPJ_B Chain B, Beta galactosidase [Niallia circulans]
4CU6_A	3.70e-147	7	750	18	825	Unravellingthe multiple functions of the architecturally intricate Streptococcus pneumoniae beta-galactosidase, BgaA [Streptococcus pneumoniae TIGR4],4CU7_A Unravelling the multiple functions of the architecturally intricate Streptococcus pneumoniae beta-galactosidase, BgaA [Streptococcus pneumoniae TIGR4],4CU8_A Unravelling the multiple functions of the architecturally intricate Streptococcus pneumoniae beta-galactosidase, BgaA [Streptococcus pneumoniae TIGR4]
4CUC_A	1.01e-146	7	750	18	825	Unravellingthe multiple functions of the architecturally intricate Streptococcus pneumoniae beta-galactosidase, BgaA. [Streptococcus pneumoniae TIGR4]
5DMY_A	1.11e-137	2	771	36	884	Beta-galactosidase- construct 33-930 [Bifidobacterium bifidum],5DMY_B Beta-galactosidase - construct 33-930 [Bifidobacterium bifidum],5DMY_C Beta-galactosidase - construct 33-930 [Bifidobacterium bifidum]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
A7LXS9	8.24e-153	29	764	70	843	Beta-galactosidase BoGH2A OS=Bacteroides ovatus (strain ATCC 8483 / DSM 1896 / JCM 5824 / BCRC 10623 / CCUG 4943 / NCTC 11153) OX=411476 GN=BACOVA_02645 PE=1 SV=1
T2KM09	2.07e-89	7	758	51	809	Putative beta-glucuronidase OS=Formosa agariphila (strain DSM 15362 / KCTC 12365 / LMG 23005 / KMM 3901 / M-2Alg 35-1) OX=1347342 GN=BN863_22050 PE=2 SV=2
T2KPJ7	1.12e-45	32	613	81	673	Putative beta-glucuronidase OS=Formosa agariphila (strain DSM 15362 / KCTC 12365 / LMG 23005 / KMM 3901 / M-2Alg 35-1) OX=1347342 GN=BN863_21970 PE=2 SV=1
P77989	3.86e-38	30	757	32	725	Beta-galactosidase OS=Thermoanaerobacter pseudethanolicus (strain ATCC 33223 / 39E) OX=340099 GN=lacZ PE=3 SV=2
T2KN75	9.59e-31	11	708	35	736	Beta-glucuronidase OS=Formosa agariphila (strain DSM 15362 / KCTC 12365 / LMG 23005 / KMM 3901 / M-2Alg 35-1) OX=1347342 GN=BN863_22060 PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000067	0.000000	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

There is no transmembrane helices in MGYG000002609_00719.