CAZyme Information: MGYG000002531_00577

Basic Information

• Species: Pseudobutyrivibrio fibrisolvens_A
• Lineage: Bacteria; Firmicutes_A; Clostridia; Lachnospirales; Lachnospiraceae; Pseudobutyrivibrio; Pseudobutyrivibrio fibrisolvens_A
• CAZyme ID: MGYG000002531_00577
• CAZy Family: GT9
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
364		41284.62	6.6192

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000002531	3116879	Isolate	not provided	not provided

• Gene Location: Start: 542060; End: 543154 Strand: -

Full Sequence      

MVWSTAFIRELRRNIPNTKIDIIINNNHKELFKECSYVDTVFGYECNDLDKPVDEKVLIK
AKEYYNNRIKIKYDAVFLPRLLPSGLADAWENVILALESGAKIRYAHSYYVSDRDCAIVD
SVECYFTNISKHLFGQSEVRSKLDLITDNRGIVLDEKMELWLSNVDKEYANKIIEPNGIK
VAVGLVGSNPNRCWSPCRYTEVIKRINDKYKDKDIKFVLMGGDDAIDASKIILENIDINL
INLVNKTSLSRAISVVNMCDIYLGSDTGLMHVAAAFKKYVIELSAASEETPSYWGSSPER
TGPWKTKFQILKAEKPLPGCGCYCSMPVPHCINQITVDDVVAALENALLEKLYKRSTCPL
RIQE

Enzyme Prediction     

No EC number prediction in MGYG000002531_00577.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GT9	70	320	7.8e-37	0.8711111111111111

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
cd03789	GT9_LPS_heptosyltransferase	9.00e-33	1	346	13	277	lipopolysaccharide heptosyltransferase and similar proteins. Lipopolysaccharide heptosyltransferase (2.4.99.B6) is involved in the biosynthesis of lipooligosaccharide (LOS). Lipopolysaccharide (LPS) is a major component of the outer membrane of gram-negative bacteria. LPS heptosyltransferase transfers heptose molecules from ADP-heptose to 3-deoxy-D-manno-octulosonic acid (KDO), a part of the inner core component of LPS. This family also contains lipopolysaccharide 1,2-N-acetylglucosaminetransferase EC 2.4.1.56 and belongs to the GT-B structural superfamily of glycoslytransferases, which have characteristic N- and C-terminal domains each containing a typical Rossmann fold. The two domains have high structural homology despite minimal sequence homology. The large cleft that separates the two domains includes the catalytic center and permits a high degree of flexibility.
COG0859	RfaF	2.11e-26	1	352	15	334	ADP-heptose:LPS heptosyltransferase [Cell wall/membrane/envelope biogenesis].
pfam01075	Glyco_transf_9	7.30e-13	188	305	115	221	Glycosyltransferase family 9 (heptosyltransferase). Members of this family belong to glycosyltransferase family 9. Lipopolysaccharide is a major component of the outer leaflet of the outer membrane in Gram-negative bacteria. It is composed of three domains; lipid A, Core oligosaccharide and the O-antigen. All of these enzymes transfer heptose to the lipopolysaccharide core.
TIGR02195	heptsyl_trn_II	2.00e-09	190	349	187	334	lipopolysaccharide heptosyltransferase II. This family consists of examples of ADP-heptose:LPS heptosyltransferase II, an enzyme of LPS inner core region biosynthesis. LPS, composed of lipid A, a core region, and O antigen, is found in the outer membrane of Gram-negative bacteria. [Cell envelope, Biosynthesis and degradation of surface polysaccharides and lipopolysaccharides]
PRK10916	PRK10916	3.20e-06	241	312	244	306	ADP-heptose--LPS heptosyltransferase RfaF.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
CBK73526.1	8.25e-273	1	364	1	364
ADL13654.1	1.09e-18	1	348	22	339
BAJ73544.1	1.81e-17	175	350	234	409
BBO72554.1	1.91e-17	2	348	24	346
AEB99318.1	5.02e-17	8	364	69	430

PDB Hits     

has no PDB hit.

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
P25742	8.17e-06	1	350	7	333	Lipopolysaccharide core heptosyltransferase RfaQ OS=Escherichia coli (strain K12) OX=83333 GN=rfaQ PE=3 SV=2

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000057	0.000000	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

There is no transmembrane helices in MGYG000002531_00577.