CAZyme Information: MGYG000002403_05073

Basic Information

• Species: Robinsoniella peoriensis
• Lineage: Bacteria; Firmicutes_A; Clostridia; Lachnospirales; Lachnospiraceae; Robinsoniella; Robinsoniella peoriensis
• CAZyme ID: MGYG000002403_05073
• CAZy Family: GH136
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
1512	MGYG000002403_132|CGC6	164964.91	4.3875

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000002403	7202103	Isolate	not provided	not provided

• Gene Location: Start: 251779; End: 256317 Strand: -

Full Sequence      

MRLKKQWTARAMSVLLAGAMTVSGIPATAQAAEQETVTESVTNTVTAVQHGYQAVINEYV
PATDGTIYYVDSETGSDENDGTTADRAFKTLEKVNAVNLQPGDSILLKKGSIFDNQRLYP
KGKGTEERPILISSYGEGNMPVINAGGFRKTGAANPDGTPGYAGYKEAILIENMEYTTVT
GIEVTNDDDFTVNWQSGASNKDDELPRRLGIHVTVDERAVETYKGLDERSYKGIVIDGCY
VHDVDGNENRGVNKVDGGIGIEVIFSSKAGVYPYFDGVTIQNNQIEKCDRTGIKGIRISD
LDNFRHNDPSKSDYDPNNDADNNGANDGSRSNNIRYKDQAQRNYKVLNNDVSDIGGDGIL
VCETKGAVVENNLLHNHTMRATPGANAGIWSWNAFDTMFRYNESYGGPSYNQDGCSYDSD
YYSAGTIFEYNYSHDTPMGFMLLMGGNDTDVIRYNLSQNDGMVMRHIAAGSSTPSYIYNN
VFYYNGDNWKWSLGSPNSVASNWNFYNNIFYNTSTKSNGEWGNVDWSKAGTANNLVYNAT
GTYSEKEIAGAIHGDPEFVKPGGGADRADGNKDNIWSSLEAYQIKNSSIAIGAGTYVNVV
PQTKQSNAGQWDYTSDRNATQDFYGNPLYNGVPDIGIHESADTGTRDFTIEKNAVYRVMN
TDQMSYLEISGNELALNKEEDNNQGFTFVGSQDGVKIRIWDSEANSYQYLAIVNDTFALS
ETDQTVWQTEDLHNGLFRLHDGNQYLSLADGKVTVEAPADTAAQQWYLKLAEHSSSFNSG
GGEIEGYSPDQAFDAEKGKSGYYGEVKQISGTAAGADEVYNTALTGAEFGYKIYAKQGEY
NVKLNFAELEGVKNRTFDILVNGEQYDVYTITDTASVKEIGSVYPVDGIIDIRLAAAYNG
DGIETDAILNGISLERNAMSDVNMRLNCGGDASDGLYADARFPAGGSGYYGENTETEAGN
RLPDPDAGMGTVLKSARSGQDFGYKFKAQPGNYRVKLYFNEVNDEEHIFSIALNGKTIKE
NYSIKKEAGGKEKAVDITAYTESQNGLIDIHFTAEEGSLAMVNAIVVEPYAGIEGDNLAV
GKTVSASSEKGSNTADKAIDNHQSTRWSAEDSGDQWYQVDLGKNYMINNVAADWTPGAYA
TKYRVEISENGVDFTTAASVNAALPGLNVTTLDSEVARYIRITGEEKNASWGISMTELGV
YGTEVRGNARVNVATNHADGTENNYDLIIGAQNIYKRYKTMTVKMSYDSHLAEFVEAKTD
LNTGALVNVSTSQPDLSNPDNAVVTSVFSIKKADGFMETAEILSARFKALSQEKTPIYTE
IALTNAAGHVTTLPVKKVFIPDEVSKEQLAAFIAEAVVLHDEAEVGDMRGQYPKEAKDAF
HEAIRSAQETAGNPDAAAESYKESYLALEEAVTVFKNSVVVAKYTNYHRDYNIDTEANYS
GGTAAVENQALKLSLSGGGKVHVVDQDSTPLKEGTYYTRFSVNNLSDQTRFVIKLTTGNE
IVVGIDGNSNWF

Enzyme Prediction     

No EC number prediction in MGYG000002403_05073.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH136	65	639	8.8e-153	0.9959266802443992
CBM32	1084	1198	9.5e-24	0.9274193548387096
CBM57	924	1065	9.5e-19	0.9863945578231292

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
pfam00754	F5_F8_type_C	1.98e-17	1083	1195	1	123	F5/8 type C domain. This domain is also known as the discoidin (DS) domain family.
pfam11721	Malectin	1.19e-14	922	1054	1	142	Di-glucose binding within endoplasmic reticulum. Malectin is a membrane-anchored protein of the endoplasmic reticulum that recognizes and binds Glc2-N-glycan. It carries a signal peptide from residues 1-26, a C-terminal transmembrane helix from residues 255-274, and a highly conserved central part of approximately 190 residues followed by an acidic, glutamate-rich region. Carbohydrate-binding is mediated by the four aromatic residues, Y67, Y89, Y116, and F117 and the aspartate at D186. NMR-based ligand-screening studies has shown binding of the protein to maltose and related oligosaccharides, on the basis of which the protein has been designated "malectin", and its endogenous ligand is found to be Glc2-high-mannose N-glycan.
cd00057	FA58C	1.90e-11	1076	1202	6	143	Substituted updates: Jan 31, 2002
pfam11721	Malectin	1.14e-09	776	900	4	146	Di-glucose binding within endoplasmic reticulum. Malectin is a membrane-anchored protein of the endoplasmic reticulum that recognizes and binds Glc2-N-glycan. It carries a signal peptide from residues 1-26, a C-terminal transmembrane helix from residues 255-274, and a highly conserved central part of approximately 190 residues followed by an acidic, glutamate-rich region. Carbohydrate-binding is mediated by the four aromatic residues, Y67, Y89, Y116, and F117 and the aspartate at D186. NMR-based ligand-screening studies has shown binding of the protein to maltose and related oligosaccharides, on the basis of which the protein has been designated "malectin", and its endogenous ligand is found to be Glc2-high-mannose N-glycan.
PLN03150	PLN03150	6.39e-05	837	1067	92	359	hypothetical protein; Provisional

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
AIY85170.1	0.0	1	1512	1	1469
AQM59543.1	0.0	1	1512	1	1469
ASU31098.1	0.0	5	1512	5	1517
ANU78288.1	0.0	5	1512	5	1517
QQQ91631.1	0.0	5	1512	5	1517

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
7V6M_A	1.18e-56	63	641	6	578	ChainA, Fibronectin type III domain-containing protein [Tyzzerella nexilis]
7V6I_A	2.82e-43	65	641	13	610	ChainA, Lacto-N-biosidase [Bifidobacterium saguini DSM 23967]
5GQC_A	1.81e-42	65	641	17	598	Crystalstructure of lacto-N-biosidase LnbX from Bifidobacterium longum subsp. longum, ligand-free form [Bifidobacterium longum subsp. longum],5GQC_B Crystal structure of lacto-N-biosidase LnbX from Bifidobacterium longum subsp. longum, ligand-free form [Bifidobacterium longum subsp. longum],5GQC_C Crystal structure of lacto-N-biosidase LnbX from Bifidobacterium longum subsp. longum, ligand-free form [Bifidobacterium longum subsp. longum],5GQC_D Crystal structure of lacto-N-biosidase LnbX from Bifidobacterium longum subsp. longum, ligand-free form [Bifidobacterium longum subsp. longum],5GQC_E Crystal structure of lacto-N-biosidase LnbX from Bifidobacterium longum subsp. longum, ligand-free form [Bifidobacterium longum subsp. longum],5GQC_F Crystal structure of lacto-N-biosidase LnbX from Bifidobacterium longum subsp. longum, ligand-free form [Bifidobacterium longum subsp. longum],5GQC_G Crystal structure of lacto-N-biosidase LnbX from Bifidobacterium longum subsp. longum, ligand-free form [Bifidobacterium longum subsp. longum],5GQC_H Crystal structure of lacto-N-biosidase LnbX from Bifidobacterium longum subsp. longum, ligand-free form [Bifidobacterium longum subsp. longum],5GQF_A Crystal structure of lacto-N-biosidase LnbX from Bifidobacterium longum subsp. longum, lacto-N-biose complex [Bifidobacterium longum subsp. longum],5GQF_B Crystal structure of lacto-N-biosidase LnbX from Bifidobacterium longum subsp. longum, lacto-N-biose complex [Bifidobacterium longum subsp. longum],5GQG_A Crystal structure of lacto-N-biosidase LnbX from Bifidobacterium longum subsp. longum, galacto-N-biose complex [Bifidobacterium longum subsp. longum],5GQG_B Crystal structure of lacto-N-biosidase LnbX from Bifidobacterium longum subsp. longum, galacto-N-biose complex [Bifidobacterium longum subsp. longum]
6KQT_A	3.74e-33	65	483	244	638	CrystalStructure of GH136 lacto-N-biosidase from Eubacterium ramulus - native protein [Eubacterium ramulus ATCC 29099]
6KQS_A	1.55e-32	65	483	244	638	CrystalStructure of GH136 lacto-N-biosidase from Eubacterium ramulus - selenomethionine derivative [Eubacterium ramulus ATCC 29099]

Swiss-Prot Hits     

has no Swissprot hit.

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.000401	0.998674	0.000230	0.000270	0.000207	0.000180

TMHMM  Annotations     

There is no transmembrane helices in MGYG000002403_05073.