CAZyme Information: MGYG000002403_01339

Basic Information

• Species: Robinsoniella peoriensis
• Lineage: Bacteria; Firmicutes_A; Clostridia; Lachnospirales; Lachnospiraceae; Robinsoniella; Robinsoniella peoriensis
• CAZyme ID: MGYG000002403_01339
• CAZy Family: CBM67
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
2029		223140.93	4.611

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000002403	7202103	Isolate	not provided	not provided

• Gene Location: Start: 3397; End: 9486 Strand: -

Full Sequence      

MKWRESICNRFWKKRKQSLALLLAVVMSVVSLEAPVFAAEINMVGEEDTSSFADDTQDTT
IYDLKTDDLVNPVGIDTANPVFSWKMNSTAMGQCQTAYQIIVAKDVEFADVYWDSQKILS
DISVGIKYAGNPLEASTTYYWKVVVWDKDGNEIQSDAASFEMGLLGNEGWNQSQWIQVGN
STEPPTSTAGETNYVVEADIQIVTTSVSLLLEATDNNNFLLWQFNNKNGYMEFKPHYRKA
GNFHTIRQMNVSQYINGKDTDVQHVKVVATNESISTYLNDHLIEEIPASDLGGIGLNGTI
GRLGFRSHSMEDEAGWMDNITLTDYSVDPDGIVLKKYDFEDGYNPFDDGAVENGRLNTKY
TGVETIALEKTEEETVEKVNYSVEADITCHMDAVSLLFNATDTSNFYMFQLNTKDQLGKV
LFKPHTWKDGSFATYSSHNKDVTSYLGSAEEFKTNSAHIKIDVTEKEIKTYINEQLIDTF
SIGELSDQNSIGIAPQIAHLGFRADLEENGTVDNFRLVDYTDNISGDIVYDYDFENENPF
LTGAVEDGKFVTKGVGILLPPQGISTFRKEVTPGDNLVSAKLYTAGLGVYDVFINGERVG
TRQDDGSVIYDELKPGYNHHSKRTIYHTYDVTQMVNGEETSVISAHVTSGWWSGQVVGFY
GKEEAFRAQLLLTYGDGSTRVIGTDRSWKTALQGPILYGDIYNGETYDANADLSFRQTGY
DDSKWTYADLNKEFNGIICPQDGPSVRVRNDLELTAQSAVVYDGAVDANENQFGRINITG
TYAPVSAFVLKAGETAVFDMGQNFAGWDEIQAEGRKGTILTMRHSEMLNDNNGLKSRGND
GAQGSIYTANLRSAKAAGRYIMNGVGIESYHSTSSFYGFRYVEVTTTQDVTIHGMKGIVV
SSVADDTGMISTSDDDVNQLISNILWGQYSNYLSVPTDCPQRDERKGWTADTQVFSTAAA
YNGDSKGFLRKYMDDMNDSQVTEGEYNGAYPDTAPYNGYGEIGQLGWGDAGIIIPYNLYK
MYGDATVIEENYSNMQDFMDIFMASTNKMGGGHNHGDWLAYESNDDEVQNLFGIAYYAWD
AAMMSEMAAVLGKTEDAERYQALYEEEKAFFQEMFVQEDGSLKRTEQTACLMALKMDLLP
DENSKAVVKQALLDNIKRNGNKLQTGFLGTAIIMQTLSDIGATDVAYQLLLQHGNPSWLY
SVDQGATTVWERWNSYTIEDGFGPVSMNSFNHYAYGAVAEWMYGYMAGIMYDTQNPGFKH
IILQPSPDQSIQKVDCTYDSAYGSIVSNWSYQNAKFNYDAVVPANTTATISIPVEDGETV
TVNGKSYTEVTAEKDGLSYIETKDNKAVFEAVSGSYHFSTGVAEYCNITLKNADTTIPCL
ISVDGSEMQVMPSGIKVEKGKAVTIKAVPVNDVDYACVGWSGDESAESSQITVTPQGNMT
LAAEFAWIGSENLAEQQPVTSNETGWDIDAWSHANLVDGILTSESQSLGYTTMQGQSPDV
DYWVEIDLGEDTDFNRIQLYPRSDTLSINGGAPNFPKDFSFEVRKENETQYDTIVTNTDY
EAAVGKPSVFTFESANARYVRLHVTKLGDPAAADRDYYFLQLAEMGIYNRDNKPVIDRAA
LEKAIEDAKTYEGKQADYTESSWKNFQDALEEAQRILSDETADQETVDTVARALNQAMKD
LIAADREPVVESVKVSPGSTVLERGSAQKFTASVIGKNEPQQTVTWSVTGNYSAATTISK
DGVLTVGIDETAALLTVRAASAVDPDKFGTATVTLKAVPPSDVKVSRISVTASANRIFIK
EKTTVRAVLLPENATNKNLNWTSSDTKVATVDNQGKVTAKKDGTVRIIATAADGSNVSGS
CSIKVVKPRVKLNASSIKLQLKKSTKALKASGLLSGDKIKSWTSKNKKIATVTKSGKITA
KKAGNTSIIVTTVKGAKAVCRIKVVKSAVKTNKITADKKKVTLKKGKSYQLKISRSPITA
TDKITYTTSASRFVSVNKKGKIVAKKKGKAVITIKTSNGKSTKVKVEVV

Enzyme Prediction     

EC	3.2.1.40

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH78	793	1311	6.7e-155	0.9841269841269841
CBM67	560	729	5.8e-24	0.875

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
pfam17389	Bac_rhamnosid6H	2.85e-125	905	1246	1	340	Bacterial alpha-L-rhamnosidase 6 hairpin glycosidase domain. This family consists of bacterial rhamnosidase A and B enzymes. L-Rhamnose is abundant in biomass as a common constituent of glycolipids and glycosides, such as plant pigments, pectic polysaccharides, gums or biosurfactants. Some rhamnosides are important bioactive compounds. For example, terpenyl glycosides, the glycosidic precursor of aromatic terpenoids, act as important flavouring substances in grapes. Other rhamnosides act as cytotoxic rhamnosylated terpenoids, as signal substances in plants or play a role in the antigenicity of pathogenic bacteria.
pfam08531	Bac_rhamnosid_N	1.53e-44	579	750	5	172	Alpha-L-rhamnosidase N-terminal domain. This family consists of bacterial rhamnosidase A and B enzymes. This domain is probably involved in substrate recognition.
pfam05592	Bac_rhamnosid	2.43e-25	795	891	7	97	Bacterial alpha-L-rhamnosidase concanavalin-like domain. This family consists of bacterial rhamnosidase A and B enzymes. L-Rhamnose is abundant in biomass as a common constituent of glycolipids and glycosides, such as plant pigments, pectic polysaccharides, gums or biosurfactants. Some rhamnosides are important bioactive compounds. For example, terpenyl glycosides, the glycosidic precursor of aromatic terpenoids, act as important flavouring substances in grapes. Other rhamnosides act as cytotoxic rhamnosylated terpenoids, as signal substances in plants or play a role in the antigenicity of pathogenic bacteria.
COG5492	YjdB	1.20e-15	1765	1940	166	329	Uncharacterized conserved protein YjdB, contains Ig-like domain [General function prediction only].
pfam17390	Bac_rhamnosid_C	5.91e-15	1256	1325	7	76	Bacterial alpha-L-rhamnosidase C-terminal domain. This family consists of bacterial rhamnosidase A and B enzymes. L-Rhamnose is abundant in biomass as a common constituent of glycolipids and glycosides, such as plant pigments, pectic polysaccharides, gums or biosurfactants. Some rhamnosides are important bioactive compounds. For example, terpenyl glycosides, the glycosidic precursor of aromatic terpenoids, act as important flavouring substances in grapes. Other rhamnosides act as cytotoxic rhamnosylated terpenoids, as signal substances in plants or play a role in the antigenicity of pathogenic bacteria.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
ADD61991.1	1.36e-248	61	1358	21	1145
BCA51920.1	1.36e-248	61	1358	21	1145
QUT40529.1	1.36e-248	61	1358	21	1145
QQA09206.1	5.49e-247	61	1358	21	1145
QUT72731.1	1.07e-246	61	1358	21	1145

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
3W5M_A	1.40e-131	573	1363	312	1033	CrystalStructure of Streptomyces avermitilis alpha-L-rhamnosidase [Streptomyces avermitilis MA-4680 = NBRC 14893],3W5N_A Crystal Structure of Streptomyces avermitilis alpha-L-rhamnosidase complexed with L-rhamnose [Streptomyces avermitilis MA-4680 = NBRC 14893]
6GSZ_A	1.11e-105	567	1322	134	856	Crystalstructure of native alfa-L-rhamnosidase from Aspergillus terreus [Aspergillus terreus]
6I60_A	1.08e-97	567	1358	179	939	Structureof alpha-L-rhamnosidase from Dictyoglumus thermophilum [Dictyoglomus thermophilum H-6-12],6I60_B Structure of alpha-L-rhamnosidase from Dictyoglumus thermophilum [Dictyoglomus thermophilum H-6-12]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
Q82PP4	1.44e-130	573	1359	312	1029	Alpha-L-rhamnosidase OS=Streptomyces avermitilis (strain ATCC 31267 / DSM 46492 / JCM 5070 / NBRC 14893 / NCIMB 12804 / NRRL 8165 / MA-4680) OX=227882 GN=SAVERM_828 PE=1 SV=1
T2KNB2	2.78e-128	540	1358	139	920	Alpha-L-rhamnosidase OS=Formosa agariphila (strain DSM 15362 / KCTC 12365 / LMG 23005 / KMM 3901 / M-2Alg 35-1) OX=1347342 GN=BN863_22090 PE=1 SV=2
P9WF03	1.39e-109	543	1326	152	892	Alpha-L-rhamnosidase OS=Alteromonas sp. (strain LOR) OX=1537994 GN=LOR_34 PE=1 SV=1
T2KPL4	2.38e-70	565	1327	185	921	Alpha-L-rhamnosidase OS=Formosa agariphila (strain DSM 15362 / KCTC 12365 / LMG 23005 / KMM 3901 / M-2Alg 35-1) OX=1347342 GN=BN863_22170 PE=2 SV=1
P33747	2.58e-12	1783	1867	35	119	Uncharacterized protein CA_P0160 OS=Clostridium acetobutylicum (strain ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787) OX=272562 GN=CA_P0160 PE=3 SV=2

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.000514	0.998699	0.000259	0.000188	0.000162	0.000149

TMHMM  Annotations     

start	end
19	41