CAZyme Information: MGYG000002396_00516

Basic Information

• Species: Bifidobacterium bifidum
• Lineage: Bacteria; Actinobacteriota; Actinomycetia; Actinomycetales; Bifidobacteriaceae; Bifidobacterium; Bifidobacterium bifidum
• CAZyme ID: MGYG000002396_00516
• CAZy Family: GH84
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
1960		210348.33	4.8199

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000002396	2311342	Isolate	South Korea	Asia

• Gene Location: Start: 629008; End: 634890 Strand: +

Full Sequence      

MRSKALGGLLAAALSLSPAVAIGVQTAYAAGGDTAATEYTLYPKPHSIRYDSGQYILRDI
NVIYDDDIDEATQDRLDEVAALKNLNVTESDAAVSGKTNVYVGVNGSDGKAETAIESKYS
PDSAIFDKTDSYFLKSDNGTISVLGKDTDASFYGLTTLYQVLGQIDSLTIRNFTVTDYAD
VVSRGFIEGYYGNPWSTQDRINLMKWGGYYKLNSYFYAPKDDPKHNSQWRTLYTQDELDT
KIKPLADAGNASKTRFVFALHPFMNNAIRFNSEANYQADLKVLQDKFAQTIGVGVRQIAI
LADDAANVGGNNYTKLLTDMVAWLKEKKKTYPDLKTTLPFVTQEYMGNGMSYFANFPKEV
QIVMTGGRVWGEVSQNFTDTFTSNVGRGPYMWINWPCSDNSKSHLIMGGYDTFLHPGVDP
SKIQGIVLNPMQQSEPSKVGIFGNATYSWNIWRSKSEADQAWQDSFSFVDHNSAVPTAAS
NALRELSKHTINQNMDSRVTALQESVDLAPALTAAKAKLADGTITAHDLTDIKAAFVTLQ
KAAKTYRAKGDAKMLGDIGKDYTGQDANEQIAPWIDCWDDTTKAALAYIAGIEAALNGDT
SSTLKEYSDAQSAFAASKKHGFYYVNHTEYAEVGVQHIVPFIKAMDSYLSNKVQQEADPN
VVTKTYISDVFTTPTSGSIEDIFDGKDSTVTVFQNPNYLHKGNYVGVKFSKATTLKSIRF
AFNGGKNHFYHSKLQTTTDGENWTDVPDATFERPKGSEEPIKVTGLNITGVTGVRLIATA
DNGDDLWLGIKGIDVNKVEKETLAPYTATGVQLENLNATYSSTKEQMIDGNPSTITYLKD
PNGDKIAAGAAVIVDLGSSKPIGEVTITGHASSPNDRPSQGVVEVSDDKATWTKLGDLRD
DVTSTVSGNVTGRYVRIRNTAAKNVWWRVAEITVTPPETADPLKSVYTNKTQHGYTATLG
ANTAELFDKSHTMLDGGQYAGLDLLAIRDLTDVELNTGNANPSLQISDNGLVWTTVEPGN
LTGKTARYVRVINDTDGAAFSVNKLKVGFSTVGKFGKLVSSDIQKRNDWGTDTRESGNAF
DGDMTTVIKFAGQPRQGNTAVFDLGQPIDITSLRIYTQDTQYDYIRDTKVQMSVDGKTWV
DAFEIGDGVSDTDTTTAFGDISDTNKKTDSNYPNVFYYGKDDIANGTGMRYLRLLTTADY
PQRALAFNEFMVNQGAYVSTEANAAFSATKVEERGHAPSNMIDGDLTTTYKPSAANGSLT
YKIDDPSDIKSFRIVQSGAASGATVTGTVYDAATGTTSGVTARAAARSTSEVTFGTLEQA
INEFKVPDGKQLLSVKIAWGDAIPEISEFITLDSADTDADINTAKAALKEKIDATVDTSA
WTANAKTAYDEAKATAQAVYGSPLVGKASIDAAASAVQSAIDAAQTKADAAAIAALRKLV
DAAVTNDGHFYTTVTFTAYTDALDEVKEALKDTDNLSTADAASLKKAVEDALAALKDSTY
QRELATIAVDSFATIDEADYTTNSYTAFKAAKKALDTLIAADGTKPAAFKTATADYTTAK
TALVNVAALKAQIAHESNYVEANYTADSWKAYSDALKVAKAKLVDGTTDSVAAALTALTA
AENALVRTTTPPDTTVLDETIADMEKVNGSEYTTDSYKALTDAIAKAKSDKVKGDAGLNQ
QNIDAMKAAKEALVSTVELKAKVAEAGKVDSSKYTTASYGVLSKLLAAKDDTTSDPVVKG
LDTLYKSGTVKELAERVAAIDAAVAKLDVRATGVKDYVDGIKLKDNDKGYYTDASYKAYS
DAYKALKKLAAAGEGEVGIAEFTEAKEAFEAAEAKLAYKSADYGKIDDLLAKVPSDLSGY
TADSVAKFEAAKKAVKRGLTIDQQSKVDAMADALEAAIKGLTLKSQAPGGNKGDGTQSGT
HKGDDISKTGADVQVFAIIIALATCAGLGAVAYARRRREA

Enzyme Prediction     

EC	3.2.1.35	3.2.1.52

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH84	184	487	6.1e-100	0.9932203389830508
CBM32	820	933	1.7e-16	0.8790322580645161

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
pfam07555	NAGidase	5.74e-118	184	486	1	293	beta-N-acetylglucosaminidase. This family has previously been described as a hyaluronidase. However, more recently it has been shown that this family has beta-N-acetylglucosaminidase activity.
pfam02838	Glyco_hydro_20b	1.08e-19	38	177	1	123	Glycosyl hydrolase family 20, domain 2. This domain has a zincin-like fold.
pfam00754	F5_F8_type_C	3.87e-08	817	932	7	127	F5/8 type C domain. This domain is also known as the discoidin (DS) domain family.
NF033930	pneumo_PspA	2.16e-04	1503	1808	32	342	pneumococcal surface protein A. The pneumococcal surface protein proteins, found in Streptococcus pneumoniae, are repetitive, with patterns of localized high sequence identity across pairs of proteins given different specific names that recombination may be presumed. This protein, PspA, has an N-terminal region that lacks a cross-wall-targeting YSIRK type extended signal peptide, in contrast to the closely related choline-binding protein CbpA which has a similar C-terminus but a YSIRK-containing region at the N-terminus.
NF033435	S-layer_Clost	0.003	1440	1790	21	381	S-layer protein SlpA. In Clostridiodes difficile, the S-layer protein precursor, SlpA, is one member of a large paralogous family of protein that share several cell wall-binding repeats. SlpA is cleaved into a larger and smaller protein. The S-layer protein itself is important to adhesion, and portions of it are highly variable, and then N-terminal and C-terminal are well-conserved.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
AXM91157.1	0.0	1	1960	1	1960
QTQ18907.1	0.0	1	1960	1	1960
ADP36543.1	0.0	1	1960	1	1960
BBA55766.1	0.0	1	1960	1	1960
BBA47544.1	0.0	1	1959	1	1959

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
6PV4_A	9.72e-204	39	650	32	645	Structureof CpGH84A [Clostridium perfringens ATCC 13124],6PV4_B Structure of CpGH84A [Clostridium perfringens ATCC 13124],6PV4_C Structure of CpGH84A [Clostridium perfringens ATCC 13124],6PV4_D Structure of CpGH84A [Clostridium perfringens ATCC 13124]
6PWI_A	4.55e-105	33	651	28	626	Structureof CpGH84D [Clostridium perfringens ATCC 13124],6PWI_B Structure of CpGH84D [Clostridium perfringens ATCC 13124]
6PV5_A	2.09e-45	38	469	39	475	Structureof CpGH84B [Clostridium perfringens ATCC 13124]
5MI4_A	6.06e-44	94	575	56	510	BtGH84mutant with covalent modification by MA3 [Bacteroides thetaiotaomicron VPI-5482],5MI5_A BtGH84 mutant with covalent modification by MA3 in complex with PUGNAc [Bacteroides thetaiotaomicron VPI-5482],5MI6_A BtGH84 mutant with covalent modification by MA3 in complex with Thiamet G [Bacteroides thetaiotaomicron VPI-5482],5MI7_A BtGH84 mutant with covalent modification by MA4 in complex with PUGNAc [Bacteroides thetaiotaomicron VPI-5482]
2CBI_A	1.55e-43	41	462	16	430	Structureof the Clostridium perfringens NagJ family 84 glycoside hydrolase, a homologue of human O-GlcNAcase [Clostridium perfringens],2CBI_B Structure of the Clostridium perfringens NagJ family 84 glycoside hydrolase, a homologue of human O-GlcNAcase [Clostridium perfringens],2CBJ_A Structure of the Clostridium perfringens NagJ family 84 glycoside hydrolase, a homologue of human O-GlcNAcase in complex with PUGNAc [Clostridium perfringens],2CBJ_B Structure of the Clostridium perfringens NagJ family 84 glycoside hydrolase, a homologue of human O-GlcNAcase in complex with PUGNAc [Clostridium perfringens],2V5C_A Family 84 glycoside hydrolase from Clostridium perfringens, 2.1 Angstrom structure [Clostridium perfringens],2V5C_B Family 84 glycoside hydrolase from Clostridium perfringens, 2.1 Angstrom structure [Clostridium perfringens],2VUR_A Chemical dissection of the link between Streptozotocin, O-GlcNAc and pancreatic cell death [Clostridium perfringens],2VUR_B Chemical dissection of the link between Streptozotocin, O-GlcNAc and pancreatic cell death [Clostridium perfringens],2X0Y_A Screening-based discovery of drug-like O-GlcNAcase inhibitor scaffolds [Clostridium perfringens],2X0Y_B Screening-based discovery of drug-like O-GlcNAcase inhibitor scaffolds [Clostridium perfringens]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
P26831	2.78e-311	39	1353	39	1352	Hyaluronoglucosaminidase OS=Clostridium perfringens (strain 13 / Type A) OX=195102 GN=nagH PE=1 SV=2
Q89ZI2	1.16e-42	94	575	66	520	O-GlcNAcase BT_4395 OS=Bacteroides thetaiotaomicron (strain ATCC 29148 / DSM 2079 / JCM 5827 / CCUG 10774 / NCTC 10582 / VPI-5482 / E50) OX=226186 GN=BT_4395 PE=1 SV=1
Q8XL08	7.74e-42	41	462	46	460	O-GlcNAcase NagJ OS=Clostridium perfringens (strain 13 / Type A) OX=195102 GN=nagJ PE=1 SV=1
Q0TR53	2.34e-41	41	462	46	460	O-GlcNAcase NagJ OS=Clostridium perfringens (strain ATCC 13124 / DSM 756 / JCM 1290 / NCIMB 6125 / NCTC 8237 / Type A) OX=195103 GN=nagJ PE=1 SV=1
O60502	2.07e-15	185	447	63	331	Protein O-GlcNAcase OS=Homo sapiens OX=9606 GN=OGA PE=1 SV=2

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.000373	0.998726	0.000189	0.000263	0.000213	0.000187

TMHMM  Annotations     

start	end
1935	1954