CAZyme Information: MGYG000002394_01927

Basic Information

• Species: Prevotella rara
• Lineage: Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Bacteroidaceae; Prevotella; Prevotella rara
• CAZyme ID: MGYG000002394_01927
• CAZy Family: GH133
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
637		73421.84	7.1278

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000002394	3788330	Isolate	Russia	Europe

• Gene Location: Start: 43984; End: 45897 Strand: +

Full Sequence      

MTNLEESLSRELLRTNRSGAYSCSTIVDCNTRKYHGLLVVPVPELDDDNHVLLSSLDVTV
VQHGAEFNLGLHKYQGNNFSPKGHKYIREFYCEKVPTTIYRVGGVLLKKEVVFQHYEHRI
LIRYTLLDAHSETTLRFKPFMAFRSVNQCTHENNTASRDFQMVDNGIKTCMYAGYPDLYM
QFSKANEFVFVPDWYRGIEYPKEQERGYSYVEDLYVPGYFEMNIKKGESIVFAASTSEIK
TSTMKHLFDKEVDDRSPRDNFFHCLVNAAHQFHNSANKTERYLLAGYPWFKCRARDTFIS
LPGLTLSIDEQDYFELVMKTAEKGLREFMQNKPLTVKITEIDQPDVLLWAIWAIQQYAKS
AGDEKCISMYGKLISDIICYIIDGKHPNLRLDTNGLLYANGHDKPITWMNSIVNGKPVVP
RSGYIVEFNALWFNALKFGAKLAKQNNNETKANQWEDLAESCKQSFVRTFLNDYGYLFDY
VDGNMMDWSVRPNMIFPVAFDYSPLSPEQKKSVLDICTRELLTPKGLRSLSPKSGGYSPV
CSGANRDYSYYQGTAWPWLGGFYMEACLKLYKRTRLSFIERQMVGYEDEMIINCLGTIPE
LFDGNPPFKARGAISYAMNVAEILRTLNLLEKYSYVK

Enzyme Prediction     

No EC number prediction in MGYG000002394_01927.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH133	276	625	5.7e-98	0.9838709677419355

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
pfam12439	GDE_N	1.08e-96	10	231	1	209	Glycogen debranching enzyme N terminal. This domain family is found in bacteria and archaea, and is typically between 218 and 229 amino acids in length. The family is found in association with pfam06202. Glycogen debranching enzyme catalyzes the debranching of amylopectin in glycogen. This is done by transferring three glucose subunits of glycogen from one parallel chain to another. This has the effect of enabling the glucose residues to become more accessible for glycolysis.
pfam06202	GDE_C	5.21e-84	283	625	21	374	Amylo-alpha-1,6-glucosidase. This family includes human glycogen branching enzyme AGL. This enzyme contains a number of distinct catalytic activities. It has been shown for the yeast homolog GDB1 that mutations in this region disrupt the enzymes Amylo-alpha-1,6-glucosidase (EC:3.2.1.33).
TIGR01561	gde_arch	1.79e-81	11	568	1	575	glycogen debranching enzyme, archaeal type, putative. The seed for this model is composed of two uncharacterized archaeal proteins from Methanosarcina acetivorans and Sulfolobus solfataricus. Trusted cutoff is set so that essentially only archaeal members hit the model. The notable exceptions to archaeal membership are the Gram positive Clostridium perfringens which scores much better than some other archaea and the Cyanobacterium Nostoc sp. which scores just above the trusted cutoff. Noise cutoff is set to exclude the characterized eukaryotic glycogen debranching enzyme in S. cerevisiae. These cutoffs leave the prokaryotes Porphyromonas gingivalis and Deinococcus radiodurans below trusted but above noise. Multiple alignments including these last two species exhibit sequence divergence which may suggest a subtly different function for these prokaryotic proteins. [Energy metabolism, Biosynthesis and degradation of polysaccharides]
COG3408	GDB1	1.23e-69	10	627	5	603	Glycogen debranching enzyme (alpha-1,6-glucosidase) [Carbohydrate transport and metabolism].
TIGR01531	glyc_debranch	3.77e-12	283	574	1023	1401	glycogen debranching enzymye. glycogen debranching enzyme possesses two different catalytic activities; oligo-1,4-->1,4-glucantransferase (EC 2.4.1.25) and amylo-1,6-glucosidase (EC 3.2.1.33). Site directed mutagenesis studies in S. cerevisiae indicate that the transferase and glucosidase activities are independent and located in different regions of the polypeptide chain. Proteins in this model belong to the larger alpha-amylase family. The model covers eukaryotic proteins with a seed composed of human, nematode and yeast sequences. Yeast seed sequence is well characterized. The model is quite rigorous; either query sequence yields large bit score or it fails to hit the model altogether. There doesn't appear to be any middle ground. [Energy metabolism, Biosynthesis and degradation of polysaccharides]

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
ALO49804.1	0.0	1	635	1	637
QUB46521.1	0.0	1	635	11	647
QNT65228.1	0.0	1	635	11	647
QUB64833.1	0.0	1	635	11	647
QUB68736.1	0.0	1	635	11	647

PDB Hits     

has no PDB hit.

Swiss-Prot Hits     

has no Swissprot hit.

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000058	0.000001	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

There is no transmembrane helices in MGYG000002394_01927.