CAZyme Information: MGYG000002391_03412

Basic Information

• Species: Oceanobacillus caeni
• Lineage: Bacteria; Firmicutes; Bacilli; Bacillales_D; Amphibacillaceae; Oceanobacillus; Oceanobacillus caeni
• CAZyme ID: MGYG000002391_03412
• CAZy Family: GT51
• CAZyme Description: Monofunctional biosynthetic peptidoglycan transglycosylase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
963		107635.87	5.441

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000002391	3564572	Isolate	not provided	not provided

• Gene Location: Start: 2635; End: 5526 Strand: +

Full Sequence      

MKLKEMFHQLKRKSKSAWNTFRNYEFINKVSTIWKTGKIQKTSRITYDVVWNIILVFLII
GFIGAFFVGGVGAGFFASLIKDEPVRSYADMKEDIYNYDETTKLYFADNIYLGDVQTDLY
REETTLDKISPTLIQAVIATEDEYFNEHQGIVPKAIFRALYQEVSNSNMQSGGSTLTQQL
IKNQILTDEVSFERKAKEILLALRLERFFEKDEILEAYLNIVPYGRNSSGGNIAGIQTAA
QGIFGINADEVNLAQAAYLAGLPQSPSYYTPFKNNGGLKDEDGIKPGLNRMKIVLNRMLD
MDYITKEEYEEAINYDVVADFKEEDKSPHKKYPILTREIQSRATDIIMYHLAEKDGHSKE
DLEKDKELKKKYSALASKAIQSEGYKVHSTIDKKMYDAFQEIAANYQYYGNDKNTVVNKK
TGETKLQSIQAAAVLIDNKTGRIISFLGNRNYSEENQTNYATAKRQIGSTAKPLAVYAPA
MELGKVQPSTTIVDEPRSWGSWSPKNYGGGNYGTVTVRKALASSYNIPAVITYEKIISQD
PASYLEKMGVTSLVEADHQNLSFALGASENGISVEENINAYATFGNNGKFADGYMIDKIT
TADGDVVYEHETKPVDVFSPQTTYLTIDMMRDVINSGTGAYVKSRLKYGGVDWAGKTGTS
QNYDDAWFIATNPNVTFGTWLGYEIPDSIYDSSSSLSYSQRTQNLWAQLINAASDINPEL
VAPSERFKQPDGIVSRSYCSISGDLASDLCQKAGLVKSDIYNSKYVPTKVDDSLIAGSQI
VIAGKAVAAGSDTPKEFTLGNGLMFNPEWLERKGINNVSQLIPRTDTDKWQQIGAPKTRL
ASYKIENDGKAPLAPTSVNYKDNKLKWNKPDSSDIVGYRIYRKSNSDGNYKLVGNTSNTE
FSVEDDNATYQVKAVDYFGLESSPSNEIVIGKDPKSADESESKKAKEEKEQKKKEEKKKE
KQE

Enzyme Prediction     

No EC number prediction in MGYG000002391_03412.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GT51	120	298	1.7e-56	0.9265536723163842

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
COG0744	MrcB	1.11e-168	48	683	4	571	Membrane carboxypeptidase (penicillin-binding protein) [Cell wall/membrane/envelope biogenesis].
TIGR02074	PBP_1a_fam	3.83e-154	120	686	1	504	penicillin-binding protein, 1A family. Bacterial that synthesize a cell wall of peptidoglycan (murein) generally have several transglycosylases and transpeptidases for the task. This family consists of bifunctional transglycosylase/transpeptidase penicillin-binding proteins (PBP). In the Proteobacteria, this family includes PBP 1A but not the paralogous PBP 1B (TIGR02071). This family also includes related proteins, often designated PBP 1A, from other bacterial lineages. [Cell envelope, Biosynthesis and degradation of murein sacculus and peptidoglycan]
COG5009	MrcA	1.37e-116	53	752	4	752	Membrane carboxypeptidase/penicillin-binding protein [Cell wall/membrane/envelope biogenesis].
COG4953	PbpC	2.79e-93	118	682	55	534	Membrane carboxypeptidase/penicillin-binding protein PbpC [Cell wall/membrane/envelope biogenesis].
pfam00912	Transgly	2.96e-70	118	299	10	177	Transglycosylase. The penicillin-binding proteins are bifunctional proteins consisting of transglycosylase and transpeptidase in the N- and C-terminus respectively. The transglycosylase domain catalyzes the polymerization of murein glycan chains.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
AIF43181.1	0.0	25	944	9	942
APC47999.1	0.0	25	943	9	941
AVQ99658.1	0.0	22	931	6	924
ALX48376.1	0.0	1	941	1	951
QKY69716.1	0.0	1	928	1	937

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
2JE5_A	5.37e-120	70	743	3	662	StructuralAnd Mechanistic Basis Of Penicillin Binding Protein Inhibition By Lactivicins [Streptococcus pneumoniae R6],2JE5_B Structural And Mechanistic Basis Of Penicillin Binding Protein Inhibition By Lactivicins [Streptococcus pneumoniae R6]
7U4H_A	2.33e-54	80	753	2	796	ChainA, Penicillin-binding protein 1A (Pbp1a) [Chlamydia trachomatis D/UW-3/CX],7U4H_B Chain B, Penicillin-binding protein 1A (Pbp1a) [Chlamydia trachomatis D/UW-3/CX]
2FFF_B	2.42e-53	338	743	10	397	OpenForm of a Class A Transpeptidase Domain [Streptococcus pneumoniae]
2XD1_A	3.21e-53	338	743	49	436	ACTIVESITE RESTRUCTURING REGULATES LIGAND RECOGNITION IN CLASS A PENICILLIN-BINDING PROTEINS [Streptococcus pneumoniae R6],2XD1_B ACTIVE SITE RESTRUCTURING REGULATES LIGAND RECOGNITION IN CLASS A PENICILLIN-BINDING PROTEINS [Streptococcus pneumoniae R6]
2BG1_A	3.21e-53	338	743	49	436	Activesite restructuring regulates ligand recognition in classA Penicillin-binding proteins (PBPs) [Streptococcus pneumoniae R6],2XD5_A Structural insights into the catalytic mechanism and the role of Streptococcus pneumoniae PBP1b [Streptococcus pneumoniae R6],2XD5_B Structural insights into the catalytic mechanism and the role of Streptococcus pneumoniae PBP1b [Streptococcus pneumoniae R6]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
P38050	1.76e-75	41	701	5	588	Penicillin-binding protein 1F OS=Bacillus subtilis (strain 168) OX=224308 GN=pbpF PE=2 SV=2
Q0TNZ8	1.61e-72	35	750	14	697	Penicillin-binding protein 1A OS=Clostridium perfringens (strain ATCC 13124 / DSM 756 / JCM 1290 / NCIMB 6125 / NCTC 8237 / Type A) OX=195103 GN=pbpA PE=3 SV=1
Q8XJ01	6.55e-72	78	697	52	654	Penicillin-binding protein 1A OS=Clostridium perfringens (strain 13 / Type A) OX=195102 GN=pbpA PE=3 SV=1
Q0SRL7	3.15e-71	35	750	14	697	Penicillin-binding protein 1A OS=Clostridium perfringens (strain SM101 / Type A) OX=289380 GN=pbpA PE=3 SV=1
A5I6G4	1.28e-70	62	785	20	713	Penicillin-binding protein 1A OS=Clostridium botulinum (strain Hall / ATCC 3502 / NCTC 13319 / Type A) OX=441771 GN=pbpA PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.999787	0.000233	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

start	end
49	71