CAZyme Information: MGYG000002356_04091

Basic Information

• Species: Bacillus_A wiedmannii
• Lineage: Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae_G; Bacillus_A; Bacillus_A wiedmannii
• CAZyme ID: MGYG000002356_04091
• CAZy Family: CBM50
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
265		29240.39	9.9421

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000002356	5414628	Isolate	Netherlands	Europe

• Gene Location: Start: 56863; End: 57660 Strand: +

Full Sequence      

MKLLKIKHIIPISAAAITFVCHQSTAEASTIHTVKKNDTLWGISKQYGVSIQSIKQANNK
ENDQTFIGEQLHIPGSVNSNEITLRQNAKPANISEQIIYQVQPGDSLETIAKRYNVTVQS
IKQINNIVGNKLYTGQHIKINSSISEKEKDLMARLVTAEAGGESYKGKVAVAKVILNRVN
AKGFPNTITGVIYEPIKYGYAFTPVTDGRINQPASPEAKMAVEEAISTNGIHSDWLYFYN
PKTSTDKWITTRQTVAVIGNHVFAK

Enzyme Prediction     

No EC number prediction in MGYG000002356_04091.

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
COG3773	CwlJ	1.58e-41	141	265	111	242	Cell wall hydrolase CwlJ, involved in spore germination [Cell cycle control, cell division, chromosome partitioning, Cell wall/membrane/envelope biogenesis].
pfam07486	Hydrolase_2	6.49e-41	162	264	1	101	Cell Wall Hydrolase. These enzymes have been implicated in cell wall hydrolysis, most extensively in Bacillus subtilis. For instance Bacillus subtilis steB is expressed during sporulation as an inactive form and then deposited on the cell outer cortex. During germination the the enzyme is activated and hydrolyzes the cortex. A similar role is carried out by the partially redundant Bacillus subtilis CwlJ. It is not clear whether these enzymes are amidases or peptidases.
PRK06347	PRK06347	8.40e-17	24	140	474	591	1,4-beta-N-acetylmuramoylhydrolase.
PRK06347	PRK06347	6.69e-15	24	145	325	454	1,4-beta-N-acetylmuramoylhydrolase.
PRK06347	PRK06347	6.81e-15	24	145	400	528	1,4-beta-N-acetylmuramoylhydrolase.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QKH56468.1	7.40e-183	1	265	1	265
AJG92383.1	7.40e-183	1	265	1	265
QIE38163.1	7.40e-183	1	265	1	265
ARO19422.1	7.40e-183	1	265	1	265
AJH73337.1	7.40e-183	1	265	1	265

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
4F55_A	5.42e-29	135	265	2	128	CrystalStructure of the Catalytic Domain of the Bacillus cereus SleB Protein [Bacillus cereus ATCC 14579]
4FET_A	3.68e-26	130	265	91	222	Catalyticdomain of germination-specific lytic tansglycosylase SleB from Bacillus anthracis [Bacillus anthracis],4FET_B Catalytic domain of germination-specific lytic tansglycosylase SleB from Bacillus anthracis [Bacillus anthracis]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
O31685	9.45e-36	145	264	92	207	Uncharacterized protein YkvT OS=Bacillus subtilis (strain 168) OX=224308 GN=ykvT PE=2 SV=1
P0A3V1	1.59e-26	130	265	128	259	Spore cortex-lytic enzyme OS=Bacillus cereus OX=1396 GN=sleB PE=1 SV=1
P0A3V0	1.59e-26	130	265	128	259	Spore cortex-lytic enzyme OS=Bacillus cereus (strain ATCC 14579 / DSM 31 / CCUG 7414 / JCM 2152 / NBRC 15305 / NCIMB 9373 / NCTC 2599 / NRRL B-3711) OX=226900 GN=sleB PE=1 SV=1
P59105	4.50e-25	138	265	153	276	Spore cortex-lytic enzyme OS=Oceanobacillus iheyensis (strain DSM 14371 / CIP 107618 / JCM 11309 / KCTC 3954 / HTE831) OX=221109 GN=sleB PE=3 SV=1
P50739	7.59e-23	145	265	189	305	Spore cortex-lytic enzyme OS=Bacillus subtilis (strain 168) OX=224308 GN=sleB PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.000417	0.944038	0.054863	0.000245	0.000219	0.000197

TMHMM  Annotations     

There is no transmembrane helices in MGYG000002356_04091.