CAZyme Information: MGYG000002329_00863

Basic Information

• Species: Dielma sp001305055
• Lineage: Bacteria; Firmicutes; Bacilli; Erysipelotrichales; Erysipelotrichaceae; Dielma; Dielma sp001305055
• CAZyme ID: MGYG000002329_00863
• CAZy Family: GT35
• CAZyme Description: Maltodextrin phosphorylase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
753		86069.84	5.6268

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000002329	3357599	Isolate	not provided	not provided

• Gene Location: Start: 104567; End: 106828 Strand: -

Full Sequence      

MTLEKQLNDLFEKASTSFEEASNKQYYYALLSVLKQRLADTPIIQGKKKLYYISAEFLIG
KLLSNNLINLNLYDEAKTLLESHGRSLAEIEEIEPEPSLGNGGLGRLAACFLDSVATLGL
AGDGIGLCYHFGLFKQQFVNHQQKEEINPWIEKDSWLREREVTFTVPFGGFSLDSKLYDI
DVVGYENGKNQLHLFDIQKVDESIVHDGIDFDKGNLLRNLTLFLYPDDSDEAGRMLRIYQ
QYFMVSNGAQYILMNAKKEGLDLHKLNEFVTIQINDTHPTLVIPELIRLLMLEGIDLDEA
IAITSKTCAYTNHTILAEALETWPRRFLEEAVPHLMPIIEALDQRVKAHYDDPSVAILDD
ADSVHMAHIDIHYGYSVNGVAAIHTEILKKTELKPFYQIYPEKFNNKTNGITFRRWLYAC
NPELDQWLNQWIGSEYKKDAAKLEQLLTFKDNKQCLDELAAIKQTKKKQLAQMLKKTQNI
DLDSESVFCIQIKRLHEYKRQQMNALYIIHKYFEIKAGKLPARPITMIFGAKAAPAYIIA
KDIIHLILCLSEFIAKDPDVAPYLKIVMVENYNVTLAEKLIPACDISEQISLASKEASGT
GNMKFMLNGAVTLGTADGANVEIAELVGEQNIYTFGESSEQVIAHYQNHDYISKKIYKQD
SHIKQCVDFIVSKELCSIGSRKILRQLQQELINKDWFMTLLDLNAYIAEKDRCLADYEKH
EEWTKKMLVNIAKAGFFSSDRTIAQYNQDIWKL

Enzyme Prediction     

EC	2.4.1.1

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GT35	80	753	3.2e-222	0.9955489614243324

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
COG0058	GlgP	0.0	10	753	17	748	Glucan phosphorylase [Carbohydrate transport and metabolism].
PRK14985	PRK14985	0.0	16	752	24	795	maltodextrin phosphorylase; Provisional
TIGR02093	P_ylase	0.0	10	753	5	794	glycogen/starch/alpha-glucan phosphorylases. This family consists of phosphorylases. Members use phosphate to break alpha 1,4 linkages between pairs of glucose residues at the end of long glucose polymers, releasing alpha-D-glucose 1-phosphate. The nomenclature convention is to preface the name according to the natural substrate, as in glycogen phosphorylase, starch phosphorylase, maltodextrin phosphorylase, etc. Name differences among these substrates reflect differences in patterns of branching with alpha 1,6 linkages. Members include allosterically regulated and unregulated forms. A related family, TIGR02094, contains examples known to act well on particularly small alpha 1,4 glucans, as may be found after import from exogenous sources. [Energy metabolism, Biosynthesis and degradation of polysaccharides]
cd04300	GT35_Glycogen_Phosphorylase	0.0	10	753	8	795	glycogen phosphorylase and similar proteins. This is a family of oligosaccharide phosphorylases. It includes yeast and mammalian glycogen phosphorylases, plant starch/glucan phosphorylase, as well as the maltodextrin phosphorylases of bacteria. The members of this family catalyze the breakdown of oligosaccharides into glucose-1-phosphate units. They are important allosteric enzymes in carbohydrate metabolism. The allosteric control mechanisms of yeast and mammalian members of this family are different from that of bacterial members. The members of this family belong to the GT-B structural superfamily of glycoslytransferases, which have characteristic N- and C-terminal domains each containing a typical Rossmann fold. The two domains have high structural homology despite minimal sequence homology. The large cleft that separates the two domains includes the catalytic center and permits a high degree of flexibility.
pfam00343	Phosphorylase	0.0	98	753	19	661	Carbohydrate phosphorylase. The members of this family catalyze the formation of glucose 1-phosphate from one of the following polyglucoses; glycogen, starch, glucan or maltodextrin.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QRT50443.1	0.0	10	753	9	752
ASW43237.1	0.0	10	753	10	752
QYX28643.1	0.0	3	753	2	753
CBL08110.1	0.0	13	753	23	763
VCV23296.1	0.0	13	753	32	772

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
4L22_A	4.42e-315	12	751	14	755	Crystalstructure of putative glycogen phosphorylase from Streptococcus mutans [Streptococcus mutans UA159]
2C4M_A	1.20e-172	14	753	21	788	Starchphosphorylase: structural studies explain oxyanion-dependent kinetic stability and regulatory control. [Corynebacterium callunae],2C4M_B Starch phosphorylase: structural studies explain oxyanion-dependent kinetic stability and regulatory control. [Corynebacterium callunae],2C4M_C Starch phosphorylase: structural studies explain oxyanion-dependent kinetic stability and regulatory control. [Corynebacterium callunae],2C4M_D Starch phosphorylase: structural studies explain oxyanion-dependent kinetic stability and regulatory control. [Corynebacterium callunae]
2FFR_A	2.42e-167	21	751	35	814	Crystallographicstudies on N-azido-beta-D-glucopyranosylamine, an inhibitor of glycogen phosphorylase: comparison with N-acetyl-beta-D-glucopyranosylamine [Oryctolagus cuniculus]
7TM7_A	2.61e-167	17	752	34	801	ChainA, Alpha-1,4 glucan phosphorylase [Klebsiella pneumoniae subsp. pneumoniae HS11286],7TM7_B Chain B, Alpha-1,4 glucan phosphorylase [Klebsiella pneumoniae subsp. pneumoniae HS11286]
2GJ4_A	6.59e-167	21	751	35	814	Structureof rabbit muscle glycogen phosphorylase in complex with ligand [Oryctolagus cuniculus]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
P29849	0.0	20	753	18	751	Maltodextrin phosphorylase OS=Streptococcus pneumoniae serotype 4 (strain ATCC BAA-334 / TIGR4) OX=170187 GN=malP PE=3 SV=2
P39123	6.16e-170	9	751	10	792	Glycogen phosphorylase OS=Bacillus subtilis (strain 168) OX=224308 GN=glgP PE=2 SV=1
Q3B7M9	2.23e-166	21	751	47	826	Glycogen phosphorylase, brain form OS=Bos taurus OX=9913 GN=PYGB PE=2 SV=3
Q5MIB6	6.25e-166	21	751	47	826	Glycogen phosphorylase, brain form OS=Ovis aries OX=9940 GN=PYGB PE=2 SV=3
P00489	6.25e-166	21	751	47	826	Glycogen phosphorylase, muscle form OS=Oryctolagus cuniculus OX=9986 GN=PYGM PE=1 SV=3

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000054	0.000000	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

There is no transmembrane helices in MGYG000002329_00863.