CAZyme Information: MGYG000002292_01208

Basic Information

• Species: TF01-11 sp003529475
• Lineage: Bacteria; Firmicutes_A; Clostridia; Lachnospirales; Lachnospiraceae; TF01-11; TF01-11 sp003529475
• CAZyme ID: MGYG000002292_01208
• CAZy Family: GH5
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
1448		153653.28	7.6975

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000002292	3137432	Isolate	China	Asia

• Gene Location: Start: 51268; End: 55614 Strand: +

Full Sequence      

MTKKWRRVYAWVLTVAMVLSMANLPITIAKAASTQNLTVKSGATSVTIAKNEYGDDYIGD
MFFNIPDALKTKSAISSNKVTSMTVKITIKSFTQGSGAKAQAFIFAQPDASGDWNWNQSS
TAELVTGSQLTLTYSFADMDWKGGTTLGNLGVRFANAAEGSTVSYSVDSAVINMADSGSS
ATSKPSSATSKPSSTASAGGNVSTDQIAITRSVGSGTNDYYAEYSFSITNNSSETVRGIQ
ILIPTSGSVDVGYVEGFSAVYDAALGGVVAYYSTEIAAGATVSSSSNKVGFGKQPSISVG
KSNVIAVNCAGPSTGDELNYELTGRKDVAYADTPVGRHGKLSVQKVDDYAAPIMVDQNGV
PTQLRGASTHGMHWFPQYVNQNAFQTLRDDWGINMVRLVCYPRDGGSVGYLTGGDSTKQQ
LDTLIQNGVDYATKLGMYALVDWHVHAYNPNEYLKEAKIFFTKYATMYKDHDNVLYEICN
EPTGTNWYSGNGKDLYTYCSEVIKTIRAIDPDAIIICGTNTWSQDVDQVAAKPMKALGYE
NIMYTFHFYSATHKENLMEKVRLATKDGTPIFVTEFGICSADGNGSYDAENADRWIALLD
ELNISFACWSYSNCNEKSAYFKSSCSNAGGDWTADDLTTTGKWLINTCRAHEEKENASYP
AVSPSAEPTKAPTVTVKPTAEPTKAPTVTEKPTTEPTKAPTVTVKPTAEPTKAPTVTVKP
TAEPTKAPTVTEKPTTEPTKAPTVTEKPTAEPTNKPTVTTAPDKGIQEAPSTALTIVART
STSLTVQATVAAPAGKSYEYTIDGTSWQTQTTFTDLQPATSYTVSVRYAETDNYQASMIS
DHTLTGGTLVEDVYKIDLSKLDDTTYVDGMFSKDDATGKSYAASYDASSNELTLSGTDHT
FELVADNAKVKIILKKGVSAALCGVTCGNISSEDDTSLEMVRGSNTTGSIEAANVIVSGG
TNNTEQIMADTIAVKGGSLTAVAAKESNKTALSADKITITDGEVTAIGDGTGGALYADSK
ISLIGGTLTVKAGPNKTEKSAIDVKSDESSVILVGDIKIINQTDGALGTDDLFSKETIST
GGTTTKLVTVTFDTVDEVWTVTVEQGSDIILPNKPKENLILRWYSDREVQGYAPGIIYTV
QENTYFCAKYFDKAPVEPSSKPTVAPSSEPTVTPSSEPTVVPSSEPTVEPSEEPIATPTA
EPTKAPEPTAAPIKTPTPTAEPTKAPTVTEKPTAEPTDAPAITPVPSDDPTESVAPSEKP
TVTPTQKPGIQATGIRVIASVKKVSNLPVKQKLQLAAGKSMQLTVTLLPIGAKPQKLTYT
SSKPSIAKVSGSGKIVAGKKAGTTVITIRTANGLQKKITIRVMKKAVKKIKLSGVKKLKV
GKKLKLKAKITPKKKYASAKVFWVSGNTKIATVTQSGVVKAKKKGKVKITAIATDGSGKK
KVIKLTIK

Enzyme Prediction     

EC	3.2.1.4	3.2.1.-

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH5	358	606	7e-82	0.9831223628691983

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
pfam00150	Cellulase	4.75e-62	355	615	1	272	Cellulase (glycosyl hydrolase family 5).
NF033761	gliding_GltJ	2.65e-10	660	797	396	533	adventurous gliding motility protein GltJ. Adventurous gliding motility protein GltJ, also known as AgmX, occurs in delta-proteobacteria such as Myxococcus xanthus.
pfam06390	NESP55	4.34e-10	1125	1268	88	239	Neuroendocrine-specific golgi protein P55 (NESP55). This family consists of several mammalian neuroendocrine-specific golgi protein P55 (NESP55) sequences. NESP55 is a novel member of the chromogranin family and is a soluble, acidic, heat-stable secretory protein that is expressed exclusively in endocrine and nervous tissues, although less widely than chromogranins.
PHA03269	PHA03269	1.93e-09	1154	1326	45	220	envelope glycoprotein C; Provisional
PTZ00436	PTZ00436	2.35e-09	650	796	197	351	60S ribosomal protein L19-like protein; Provisional

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
AAC06196.1	2.71e-121	316	649	11	338
CBK74991.1	7.46e-120	305	665	15	370
AGH41463.1	6.91e-118	316	649	218	545
AAC06197.1	1.44e-116	313	649	42	372
AGH40913.1	2.22e-116	313	649	61	391

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
6GJF_A	8.08e-76	330	649	2	301	Ancestralendocellulase Cel5A [synthetic construct],6GJF_B Ancestral endocellulase Cel5A [synthetic construct],6GJF_C Ancestral endocellulase Cel5A [synthetic construct],6GJF_D Ancestral endocellulase Cel5A [synthetic construct],6GJF_E Ancestral endocellulase Cel5A [synthetic construct],6GJF_F Ancestral endocellulase Cel5A [synthetic construct]
4XZB_A	4.10e-73	333	644	4	299	endo-glucanaseGsCelA P1 [Geobacillus sp. 70PC53]
4XZW_A	7.37e-73	333	644	4	298	Endo-glucanasechimera C10 [uncultured bacterium]
3PZT_A	6.55e-67	333	644	29	320	Structureof the endo-1,4-beta-glucanase from Bacillus subtilis 168 with manganese(II) ion [Bacillus subtilis subsp. subtilis str. 168],3PZT_B Structure of the endo-1,4-beta-glucanase from Bacillus subtilis 168 with manganese(II) ion [Bacillus subtilis subsp. subtilis str. 168],3PZU_A P212121 crystal form of the endo-1,4-beta-glucanase from Bacillus subtilis 168 [Bacillus subtilis subsp. subtilis str. 168],3PZU_B P212121 crystal form of the endo-1,4-beta-glucanase from Bacillus subtilis 168 [Bacillus subtilis subsp. subtilis str. 168],3PZV_A C2 crystal form of the endo-1,4-beta-glucanase from Bacillus subtilis 168 [Bacillus subtilis subsp. subtilis str. 168],3PZV_B C2 crystal form of the endo-1,4-beta-glucanase from Bacillus subtilis 168 [Bacillus subtilis subsp. subtilis str. 168],3PZV_C C2 crystal form of the endo-1,4-beta-glucanase from Bacillus subtilis 168 [Bacillus subtilis subsp. subtilis str. 168],3PZV_D C2 crystal form of the endo-1,4-beta-glucanase from Bacillus subtilis 168 [Bacillus subtilis subsp. subtilis str. 168]
5WH8_A	1.08e-66	333	644	6	303	CellulaseCel5C_n [uncultured organism]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
P15704	1.13e-72	354	663	54	350	Endoglucanase OS=Clostridium saccharobutylicum OX=169679 GN=eglA PE=3 SV=1
P22541	5.68e-66	332	646	107	405	Endoglucanase A OS=Butyrivibrio fibrisolvens OX=831 GN=celA PE=1 SV=1
Q07940	1.06e-65	361	658	18	304	Endoglucanase 4 OS=Ruminococcus albus OX=1264 GN=Eg IV PE=1 SV=1
P07983	1.89e-65	322	653	23	334	Endoglucanase OS=Bacillus subtilis OX=1423 GN=bglC PE=3 SV=2
O85465	1.01e-64	324	665	21	338	Endoglucanase 5A OS=Salipaludibacillus agaradhaerens OX=76935 GN=cel5A PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.000377	0.998798	0.000223	0.000204	0.000181	0.000167

TMHMM  Annotations     

start	end
7	29