CAZyme Information: MGYG000002227_01868

Basic Information

• Species: Eubacterium_R sp900546785
• Lineage: Bacteria; Firmicutes_A; Clostridia; Oscillospirales; Acutalibacteraceae; Eubacterium_R; Eubacterium_R sp900546785
• CAZyme ID: MGYG000002227_01868
• CAZy Family: GH2
• CAZyme Description: Beta-galactosidase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
685		77870.04	8.6329

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000002227	2038651	MAG	United States	North America

• Gene Location: Start: 10100; End: 12157 Strand: +

Full Sequence      

MKKILSLNKSWQFCRGGFPPAENAGMQEIDLPHTWNNLDGQDGGGDYFRGACCYMKKIAL
NKKGNTLYYLEFYGANSVANVYVNGARIGEHRGGYTLFRFDITGALKNGENEIAVEVSNF
PFPDVIPLTADFTFFGGIYREVNLVAVSGAHFSLDDYGSAGVYVTYPNTPQVEKRAQVTV
RAKIKRAESECKLRVSVSPAGGFAPCAGIDKPDFDAAELADNGSWTVAQAEKDINKENAE
LKLYVDSPRLWNGRKAPFRYKVQCEILEDGKITDSVERYIGFRWFTIDPQKGFFLNGRRY
PLRGVNRHQDRENMGWAITDREHEEDFALIYEMGANAIRLAHYPHHPHFYELCDRYGLLV
WAEIPFVENIGGLGLSPLETDQAKDDAVTARMLENAKQQYTELILQQLHRPSIFCWSMSN
EVRREYGETAAHMMRALNQLAHRLDPSRYTALATNHYGGDTWDSDIKGCNIYPGWYWGSA
KRFRSQAAAHIRANGGRGVAVSEYGAGSNVLHHTEKPKQPKDTVCEFHSEEWASVVHEHA
LKYFMSPKADKIWGAFVWNMFDFAIDSRNEGGVPGRNDKGLVTYDRKIKKDPFYLYKAYW
SGEGVLYITSRRFTRREKRWINVKIYSNQKEITLYVNGVRAGQKTAKPAPHSHIFVFKGV
KLRRGKNEIKAVSDSGMKDVVIWEY

Enzyme Prediction     

No EC number prediction in MGYG000002227_01868.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH2	2	597	1e-102	0.6183510638297872

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
COG3250	LacZ	9.12e-47	1	624	9	602	Beta-galactosidase/beta-glucuronidase [Carbohydrate transport and metabolism].
PRK10150	PRK10150	1.04e-36	70	602	86	594	beta-D-glucuronidase; Provisional
PRK10340	ebgA	4.61e-35	68	572	128	557	cryptic beta-D-galactosidase subunit alpha; Reviewed
pfam02836	Glyco_hydro_2_C	9.84e-29	293	600	8	298	Glycosyl hydrolases family 2, TIM barrel domain. This family contains beta-galactosidase, beta-mannosidase and beta-glucuronidase activities.
PRK09525	lacZ	1.37e-25	72	466	144	499	beta-galactosidase.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
BCI62299.1	1.16e-176	5	683	26	698
QUT74077.1	2.11e-165	1	676	25	656
AIQ56230.1	4.85e-165	1	673	1	640
QFK71524.1	1.21e-162	1	675	1	641
CEI73305.1	5.18e-161	1	675	1	642

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
6MVG_A	2.07e-144	1	657	24	631	Crystalstructure of FMN-binding beta-glucuronidase from Ruminococcus gnavus [[Ruminococcus] gnavus],6MVG_B Crystal structure of FMN-binding beta-glucuronidase from Ruminococcus gnavus [[Ruminococcus] gnavus],6MVG_C Crystal structure of FMN-binding beta-glucuronidase from Ruminococcus gnavus [[Ruminococcus] gnavus]
3CMG_A	2.50e-138	1	685	3	655	Crystalstructure of putative beta-galactosidase from Bacteroides fragilis [Bacteroides fragilis NCTC 9343]
5Z1A_A	3.66e-138	1	685	22	674	Thecrystal structure of Bacteroides fragilis beta-glucuronidase in complex with uronic isofagomine [Bacteroides fragilis NCTC 9343]
6MVH_A	6.20e-133	1	657	24	631	Crystalstructure of FMN-binding beta-glucuronidase from Roseburia hominis [Roseburia hominis],6MVH_B Crystal structure of FMN-binding beta-glucuronidase from Roseburia hominis [Roseburia hominis],6MVH_C Crystal structure of FMN-binding beta-glucuronidase from Roseburia hominis [Roseburia hominis],6MVH_D Crystal structure of FMN-binding beta-glucuronidase from Roseburia hominis [Roseburia hominis]
7KGZ_A	2.74e-132	1	682	1	621	ChainA, Beta-glucuronidase [Roseburia hominis],7KGZ_B Chain B, Beta-glucuronidase [Roseburia hominis]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
T2KPJ7	3.45e-93	6	672	55	703	Putative beta-glucuronidase OS=Formosa agariphila (strain DSM 15362 / KCTC 12365 / LMG 23005 / KMM 3901 / M-2Alg 35-1) OX=1347342 GN=BN863_21970 PE=2 SV=1
T2KM09	8.24e-44	6	643	50	666	Putative beta-glucuronidase OS=Formosa agariphila (strain DSM 15362 / KCTC 12365 / LMG 23005 / KMM 3901 / M-2Alg 35-1) OX=1347342 GN=BN863_22050 PE=2 SV=2
P77989	2.08e-40	2	450	3	412	Beta-galactosidase OS=Thermoanaerobacter pseudethanolicus (strain ATCC 33223 / 39E) OX=340099 GN=lacZ PE=3 SV=2
P26257	8.28e-39	1	450	1	413	Beta-galactosidase OS=Thermoanaerobacterium thermosulfurigenes OX=33950 GN=lacZ PE=1 SV=1
A7LXS9	1.52e-38	8	644	48	698	Beta-galactosidase BoGH2A OS=Bacteroides ovatus (strain ATCC 8483 / DSM 1896 / JCM 5824 / BCRC 10623 / CCUG 4943 / NCTC 11153) OX=411476 GN=BACOVA_02645 PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.999956	0.000083	0.000002	0.000000	0.000000	0.000001

TMHMM  Annotations     

There is no transmembrane helices in MGYG000002227_01868.