CAZyme Information: MGYG000002227_01463

Basic Information

• Species: Eubacterium_R sp900546785
• Lineage: Bacteria; Firmicutes_A; Clostridia; Oscillospirales; Acutalibacteraceae; Eubacterium_R; Eubacterium_R sp900546785
• CAZyme ID: MGYG000002227_01463
• CAZy Family: GH23
• CAZyme Description: DNA mismatch repair protein MutS

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
2739		312203.62	4.7394

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000002227	2038651	MAG	United States	North America

• Gene Location: Start: 24955; End: 33174 Strand: -

Full Sequence      

MPSKFELITKVYYDMCKEVVRSPQAWQRFLESACRNYRLRFDEQLLVFAQRSDATAVLEF
EKWNRRFGRHINRGTKGIAVFENVEKTRIKHYFDISDTSETEHARPVPIWKYKDEYEQAV
IETLENTFGELEHKNDMIDAVISAADNATEDNTLDYISDLIRSTQNSFLEELDDDSVFTV
YRTLIKNSVAYMILSRLGVSADQYFGREEFAGAVNFNTPETLNAVGFATSDIAEIALNEV
SKTVLSLEKTDRIIAKPEQVGYTETEIKNERSVSNDRTHIQNRGRSADTESDPAHAEEFD
IEQIWADEKTVSQGGTPPALLQSADNGQARQSSDGDGRESERDGAIADETDGSAGGLDGG
TQSSGSDGLGTENEQFEKPSAGDRAQRSYLPDLSGELPPFTDENLILGILSNPDDNLKQK
KTAIAKFFAETSDNTKRADFIKDVYPYRFTELILNEQRVGYVTAENGLLLWRGSYPSRTE
ESVFSWDIVAELVGQLIDEGNYLPKEKKAEPAQISLFDTADIPDEQNFTETEQVSFFTDL
GVSQQIIDEVLCIGANDQNSTLDIAMFFRRDRGTAYNAAFLKRHYGTNGTGFLFQGEHIS
VWYDESGIKVSRGDYANRSGATLVTWKQAAKRIRELLDMGRYMPQESLDKIDDKELSVVS
DRLATFIRDMRGDNAKEYLPTIRAITDNVKGGYPEEVKAVKELLSDAERFDTIFREYDDF
QNAVSDGTVSVYRYGYEYSVPYVFEIFKGMRLAPLEFKAAADFAPRRERFITEDEIKRII
RGHDAASEFRLATYSFFLSHSDEKERVQFFKDHYGWQGSYDGSDNIEYGAKGLLFSHGDL
SEPYAKAEIPWKRYVKYVEDLITGRNFLSQADLESVDDYERKQVAKAVMDFWIGVPFGNQ
KPDFYNDSDYWNSVEKIAGMLESPDDAENIQKAMLETSNAVTFEERYRDYAENGLKTIEE
YVNGTYTCFGDKSRTIAPLIKVESKPAEKETELPEWLTEYREIKAEDPQALVFYQVGDFY
ECYFDDAKTVADELDLVLTGRNAGGERISMCGFPKHSLDEYMGRFSEKGYRIAVSSAEKG
ERITTWYTPDYSEEVQKAQDTKETDTIPAFETAPKTNPYTFDLHPEIPLDERHDFDFANH
EIQDVNKKERFHRNYAAIKVLKDCQSENRFATPEEQMILSRYYGWGGIPEAFDERADTWR
TEYAMLKNILTPDEYASARKSTLTAFYTPQPVISAIYKVMENLGFKQGNILEPCCAIGNF
IGMLPESMKDSKVYGIEIDNISAGIAQQLYQSSSVMNAPFEKAELPDSFFDAVVGNVPFG
DYSLADKRYDKYHFLIHDYFFAKSLDKLRPGGVICLVTSKGTMDKESTEVRRYIAQRAEL
LGAIRLPDNTFKSNAGTEVTSDILILQKRDRIIDIEPDWIFLDEDENGIRMNKYFVDHPD
MVLGEMVMQSGRFKPESACKAYEDRTLEEQLEVAVQNIEGEISEYTLEDTQESEETYIPA
DPNVRNFSYTVVDGQIYFRENSVMREVQTSETAKNRIKGMIEIRDCVKNLLEMQTEDYSD
AAIDTEQRRLNEIYDTFVKKYGYINSRGNASAFSDDSSYFLICSLEIFKDGEFERKADMF
TKRTIQPHIAKTHADTSIEAYGISIGEKAKVDMPFMCELTGKSEEEIYSDLKGVIFLNPA
YDENSGSVSKYLPADEYLSGNVRTKLREAKRLAESDERFMVNAQALEKVQPVDLKANEIE
VHLGVTWLPLEIIDRFMYETFQTPYYTRSSIKVHYNELANVWNITNKSADNINLTVTQKY
GTSRKNAFHILEATLNLRNVRVFDYEEDENGKRVAILNKKETAIAQAKQELIKQTFKEWI
WKDPKRREKLCKLYNEKFNSIRPREYDGSHIVFEGMNPEITLRTHQKNAVARGIYGGNTL
LAHCVGAGKTYTMAAIAQESKRLGLCSKSLFVVPNHLISQWASEYLTLYPSAKILAATKK
DFETKNRKKFCARIATGDYDAVIIGHSQFEKIPVSIGRQMFTLQKQIDELVEGITELKQS
KGERFTVKQLEATKIRLESKLQRLNDQSGKDDIVTFEELGVDRLFVDEAHYYKNLFVYSK
MKNVGGISQIEAHKSSDMFMKCQYLDELTENKGITFATGTPISNSMVELYTMQRYLQYDL
LKEYGYVNFDSWAAQYGETVTAVELTPEGTGYRTKVRFARFNNLPELMAMFKECADIQTS
DMLKLPVPKANYHNISVKPSETQKEMVANLGERAEKIRAGAVKPYEDNMLNITNDGRKLA
LDQRLINPSLPDFEGSKVNACISNIYDIWKDTAAQRSTQLVFCDLSTPTAKGTAQVENSE
KQADVFTNVYDDIRKKLIAKGIPAEEIAFVHSANNDRQKAELFAKVRSGKMRILLGSTQK
MGAGTNVQDRLIASHDIDCPWRPSDLEQRAGRIIRQGNSNPEVHIYRYVTENTFDAYLYQ
LVETKQKFVGQVMTSKTPVRSIEDVDEAALSYAEIKMLATGNPHIKEKMDLDIQVQNLRM
LQSNYYAERYALEDKVTMEFPRDIARYDAQIKALEKDIQTAAEHPKTIDDCFAGMVIGGV
EYADKKKAGNAILQMMKSIKSKTETYPVGSYRGFNMEMYIALGLGATYVLAVKGAMTHNV
ELGTDAFGNLTRIDNCIDHFEAELQKAKNDLAETEKQLEIAKESLKEPFSREGELQEKQA
RLNELNALLNVDKKDNEIIDEEPEDREIKVPKKEIMMCR

Enzyme Prediction     

No EC number prediction in MGYG000002227_01463.

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
COG4646	COG4646	6.15e-65	1431	1956	1	540	Adenine-specific DNA methylase, N12 class [Replication, recombination and repair].
pfam01624	MutS_I	1.39e-26	1000	1089	7	106	MutS domain I. This domain is found in proteins of the MutS family (DNA mismatch repair proteins) and is found associated with pfam00488, pfam05188, pfam05192 and pfam05190. The MutS family of proteins is named after the Salmonella typhimurium MutS protein involved in mismatch repair; other members of the family included the eukaryotic MSH 1,2,3, 4,5 and 6 proteins. These have various roles in DNA repair and recombination. Human MSH has been implicated in non-polyposis colorectal carcinoma (HNPCC) and is a mismatch binding protein. The aligned region corresponds with globular domain I, which is involved in DNA binding, in Thermus aquaticus MutS as characterized in.
COG4646	COG4646	1.48e-25	2099	2288	441	637	Adenine-specific DNA methylase, N12 class [Replication, recombination and repair].
COG0249	MutS	3.02e-21	1000	1074	13	83	DNA mismatch repair ATPase MutS [Replication, recombination and repair].
TIGR01070	mutS1	9.14e-21	993	1074	1	83	DNA mismatch repair protein MutS. [DNA metabolism, DNA replication, recombination, and repair]

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
ASV45029.1	2.68e-316	1135	2657	1548	3139
QIW86704.1	6.72e-315	1135	2657	1616	3207
QIW86628.1	6.72e-315	1135	2657	1616	3207
AEY69616.1	9.56e-303	1148	2718	1702	3343
AXF51455.1	3.44e-300	1148	2701	1795	3419

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
5AKB_A	1.35e-12	997	1075	14	93	MutSin complex with the N-terminal domain of MutL - crystal form 1 [Escherichia coli K-12],5AKB_B MutS in complex with the N-terminal domain of MutL - crystal form 1 [Escherichia coli K-12],5AKB_E MutS in complex with the N-terminal domain of MutL - crystal form 1 [Escherichia coli K-12],5AKC_A MutS in complex with the N-terminal domain of MutL - crystal form 2 [Escherichia coli K-12],5AKC_B MutS in complex with the N-terminal domain of MutL - crystal form 2 [Escherichia coli K-12],5AKC_E MutS in complex with the N-terminal domain of MutL - crystal form 2 [Escherichia coli K-12],5AKC_F MutS in complex with the N-terminal domain of MutL - crystal form 2 [Escherichia coli K-12],5AKC_I MutS in complex with the N-terminal domain of MutL - crystal form 2 [Escherichia coli K-12],5AKC_J MutS in complex with the N-terminal domain of MutL - crystal form 2 [Escherichia coli K-12],5AKD_A MutS in complex with the N-terminal domain of MutL - crystal form 3 [Escherichia coli K-12],5AKD_B MutS in complex with the N-terminal domain of MutL - crystal form 3 [Escherichia coli K-12],5AKD_E MutS in complex with the N-terminal domain of MutL - crystal form 3 [Escherichia coli K-12],5AKD_F MutS in complex with the N-terminal domain of MutL - crystal form 3 [Escherichia coli K-12],5AKD_I MutS in complex with the N-terminal domain of MutL - crystal form 3 [Escherichia coli K-12],5AKD_J MutS in complex with the N-terminal domain of MutL - crystal form 3 [Escherichia coli K-12]
7AI6_A	1.40e-12	997	1075	14	93	ChainA, DNA mismatch repair protein MutS [Escherichia coli K-12],7AI6_B Chain B, DNA mismatch repair protein MutS [Escherichia coli K-12],7AIB_A Chain A, DNA mismatch repair protein MutS [Escherichia coli K-12],7AIB_B Chain B, DNA mismatch repair protein MutS [Escherichia coli K-12],7AIC_A Chain A, DNA mismatch repair protein MutS [Escherichia coli K-12],7AIC_B Chain B, DNA mismatch repair protein MutS [Escherichia coli K-12]
1NG9_A	1.77e-12	997	1074	14	92	ChainA, DNA mismatch repair protein MutS [Escherichia coli],1NG9_B Chain B, DNA mismatch repair protein MutS [Escherichia coli]
1OH5_A	1.77e-12	997	1074	14	92	THECRYSTAL STRUCTURE OF E. COLI MUTS BINDING TO DNA WITH A C:A MISMATCH [Escherichia coli],1OH5_B THE CRYSTAL STRUCTURE OF E. COLI MUTS BINDING TO DNA WITH A C:A MISMATCH [Escherichia coli],1OH6_A THE CRYSTAL STRUCTURE OF E. COLI MUTS BINDING TO DNA WITH AN A:A MISMATCH [Escherichia coli],1OH6_B THE CRYSTAL STRUCTURE OF E. COLI MUTS BINDING TO DNA WITH AN A:A MISMATCH [Escherichia coli],1OH7_A THE CRYSTAL STRUCTURE OF E. COLI MUTS BINDING TO DNA WITH A G:G MISMATCH [Escherichia coli],1OH7_B THE CRYSTAL STRUCTURE OF E. COLI MUTS BINDING TO DNA WITH A G:G MISMATCH [Escherichia coli],1OH8_A THE CRYSTAL STRUCTURE OF E. COLI MUTS BINDING TO DNA WITH AN UNPAIRED THYMIDINE [Escherichia coli],1OH8_B THE CRYSTAL STRUCTURE OF E. COLI MUTS BINDING TO DNA WITH AN UNPAIRED THYMIDINE [Escherichia coli],1W7A_A ATP bound MutS [Escherichia coli],1W7A_B ATP bound MutS [Escherichia coli],2WTU_A Crystal structure of Escherichia coli MutS in complex with a 16 basepair oligo containing an A.A mismatch. [Escherichia coli],2WTU_B Crystal structure of Escherichia coli MutS in complex with a 16 basepair oligo containing an A.A mismatch. [Escherichia coli],3ZLJ_A CRYSTAL STRUCTURE OF FULL-LENGTH E.COLI DNA MISMATCH REPAIR PROTEIN MUTS D835R MUTANT IN COMPLEX WITH GT MISMATCHED DNA [Escherichia coli K-12],3ZLJ_B CRYSTAL STRUCTURE OF FULL-LENGTH E.COLI DNA MISMATCH REPAIR PROTEIN MUTS D835R MUTANT IN COMPLEX WITH GT MISMATCHED DNA [Escherichia coli K-12]
3K0S_A	1.77e-12	997	1074	13	91	ChainA, DNA mismatch repair protein mutS [Escherichia coli K-12],3K0S_B Chain B, DNA mismatch repair protein mutS [Escherichia coli K-12]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
A5D2K2	4.33e-18	997	1074	2	79	DNA mismatch repair protein MutS OS=Pelotomaculum thermopropionicum (strain DSM 13744 / JCM 10971 / SI) OX=370438 GN=mutS PE=3 SV=1
Q9KAC0	2.22e-17	997	1074	8	85	DNA mismatch repair protein MutS OS=Alkalihalobacillus halodurans (strain ATCC BAA-125 / DSM 18197 / FERM 7344 / JCM 9153 / C-125) OX=272558 GN=mutS PE=3 SV=1
A4J5Q6	5.03e-17	997	1084	7	95	DNA mismatch repair protein MutS OS=Desulfotomaculum reducens (strain MI-1) OX=349161 GN=mutS PE=3 SV=1
Q5L0E5	4.53e-16	997	1074	2	79	DNA mismatch repair protein MutS OS=Geobacillus kaustophilus (strain HTA426) OX=235909 GN=mutS PE=3 SV=1
Q67NK1	1.73e-15	999	1074	12	89	DNA mismatch repair protein MutS OS=Symbiobacterium thermophilum (strain T / IAM 14863) OX=292459 GN=mutS PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000060	0.000000	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

There is no transmembrane helices in MGYG000002227_01463.