CAZyme Information: MGYG000002173_00648

Basic Information

• Species: CAG-917 sp000437555
• Lineage: Bacteria; Firmicutes_A; Clostridia_A; Christensenellales; CAG-917; CAG-917; CAG-917 sp000437555
• CAZyme ID: MGYG000002173_00648
• CAZy Family: GH2
• CAZyme Description: Beta-galactosidase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
606	MGYG000002173_7|CGC1	69712.72	7.891

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000002173	1557441	MAG	Denmark	Europe

• Gene Location: Start: 60804; End: 62624 Strand: +

Full Sequence      

MSKKVLSTQEEIKSKSKNIRVRLQSKFYDEVGEIPHNEYPRPQFERDSFFNLNGTWDFCM
QDASAEFKGYDSKILVPFSPECLLSGLDGKICQPTDVLYYKREFTLPKGFIKSRTFLNFG
AVDFECEVKINSKVVGTHKGGFVPFTFDVTDCIKEGANTIEVKVTDPTDTSYHTRAKQAL
KNGGIWYTPQSGIWQTVYLESVPEKYIADMLITPDIDSDSVRIKLELVGGGEATVKAFDG
KKEIAKAQTENGEAVLSLKDYELWSPENPKLYDLEIEYGTDKIKSYFGMRKFSVMEDKHG
KKRLALNNKIYFQSGVLDQGYWSDGMLTPPTDSAMEYDIKLMKDMGFNMLRKHIKIEPLR
WYYHCDRLGMIVWQDFVTGGRKYKFSVIGIGGFLNLPTKDTKRNYKKFSRHDEEGRAEYY
AEMETEMNYLKNCVCLGTWVPFNEGWGQFDSKIATEKVRALDPTRIIDSVSGWYDQGVGN
TDMKSLHIYFTPIKCPKDPRVCVLSEFGGYSYKERPHVFSPNKSFGYRIYSTAEKLEKAF
KKLYEKSIVKNVKKGFSATVYTQVSDVEEEINGFITYDRKVVKIPVQTVVECNEMIKKCY
RELLGE

Enzyme Prediction     

No EC number prediction in MGYG000002173_00648.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH2	43	489	1.1e-96	0.4933510638297872

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
COG3250	LacZ	1.35e-37	50	376	28	364	Beta-galactosidase/beta-glucuronidase [Carbohydrate transport and metabolism].
PRK10150	PRK10150	4.78e-25	99	372	68	352	beta-D-glucuronidase; Provisional
PRK10340	ebgA	1.00e-23	44	372	33	394	cryptic beta-D-galactosidase subunit alpha; Reviewed
PRK09525	lacZ	3.24e-19	51	372	54	410	beta-galactosidase.
pfam02837	Glyco_hydro_2_N	3.37e-17	49	203	1	169	Glycosyl hydrolases family 2, sugar binding domain. This family contains beta-galactosidase, beta-mannosidase and beta-glucuronidase activities and has a jelly-roll fold. The domain binds the sugar moiety during the sugar-hydrolysis reaction.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
ABZ98834.1	9.78e-211	33	597	2	560
ABZ95154.1	9.78e-211	33	597	2	560
AEC02401.1	3.12e-202	35	597	32	591
AXO33549.1	1.36e-193	38	597	19	581
AXH90955.1	3.12e-192	38	596	19	580

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
7SF2_A	1.10e-139	22	596	11	582	ChainA, Glycosyl hydrolase family 2, sugar binding domain protein [Bacteroides cellulosilyticus DSM 14838],7SF2_B Chain B, Glycosyl hydrolase family 2, sugar binding domain protein [Bacteroides cellulosilyticus DSM 14838],7SF2_C Chain C, Glycosyl hydrolase family 2, sugar binding domain protein [Bacteroides cellulosilyticus DSM 14838],7SF2_D Chain D, Glycosyl hydrolase family 2, sugar binding domain protein [Bacteroides cellulosilyticus DSM 14838],7SF2_E Chain E, Glycosyl hydrolase family 2, sugar binding domain protein [Bacteroides cellulosilyticus DSM 14838],7SF2_F Chain F, Glycosyl hydrolase family 2, sugar binding domain protein [Bacteroides cellulosilyticus DSM 14838]
4JKM_A	2.23e-27	45	444	11	415	CrystalStructure of Clostridium perfringens beta-glucuronidase [Clostridium perfringens str. 13],4JKM_B Crystal Structure of Clostridium perfringens beta-glucuronidase [Clostridium perfringens str. 13],6CXS_A Crystal Structure of Clostridium perfringens beta-glucuronidase bound with a novel, potent inhibitor 4-(8-(piperazin-1-yl)-1,2,3,4-tetrahydro-[1,2,3]triazino[4',5':4,5]thieno[2,3-c]isoquinolin-5-yl)morpholine [Clostridium perfringens str. 13],6CXS_B Crystal Structure of Clostridium perfringens beta-glucuronidase bound with a novel, potent inhibitor 4-(8-(piperazin-1-yl)-1,2,3,4-tetrahydro-[1,2,3]triazino[4',5':4,5]thieno[2,3-c]isoquinolin-5-yl)morpholine [Clostridium perfringens str. 13]
6D8K_A	5.22e-25	98	375	100	370	Bacteroidesmultiple species beta-glucuronidase [Bacteroides ovatus],6D8K_B Bacteroides multiple species beta-glucuronidase [Bacteroides ovatus],6D8K_C Bacteroides multiple species beta-glucuronidase [Bacteroides ovatus],6D8K_D Bacteroides multiple species beta-glucuronidase [Bacteroides ovatus]
6D89_A	1.57e-22	98	467	101	440	Bacteroidesuniformis beta-glucuronidase 1 with N-terminal loop deletion [Bacteroides uniformis],6D89_B Bacteroides uniformis beta-glucuronidase 1 with N-terminal loop deletion [Bacteroides uniformis],6D89_C Bacteroides uniformis beta-glucuronidase 1 with N-terminal loop deletion [Bacteroides uniformis],6D89_D Bacteroides uniformis beta-glucuronidase 1 with N-terminal loop deletion [Bacteroides uniformis]
6D1N_A	1.61e-22	98	467	109	448	Apostructure of Bacteroides uniformis Beta-glucuronidase 1 [Bacteroides uniformis],6D1N_B Apo structure of Bacteroides uniformis Beta-glucuronidase 1 [Bacteroides uniformis],6D41_A Bacteriodes uniformis beta-glucuronidase 1 bound to D-glucaro-1,5-lactone [Bacteroides uniformis],6D41_B Bacteriodes uniformis beta-glucuronidase 1 bound to D-glucaro-1,5-lactone [Bacteroides uniformis],6D6W_A Bacteroides uniformis beta-glucuronidase 1 bound to glucuronate [Bacteroides uniformis],6D6W_B Bacteroides uniformis beta-glucuronidase 1 bound to glucuronate [Bacteroides uniformis],6D6W_C Bacteroides uniformis beta-glucuronidase 1 bound to glucuronate [Bacteroides uniformis],6D6W_D Bacteroides uniformis beta-glucuronidase 1 bound to glucuronate [Bacteroides uniformis],6D7F_A Bacteroides uniformis beta-glucuronidase 1 bound to thiophenyl-beta-D-glucuronide [Bacteroides uniformis],6D7F_B Bacteroides uniformis beta-glucuronidase 1 bound to thiophenyl-beta-D-glucuronide [Bacteroides uniformis],6D7F_C Bacteroides uniformis beta-glucuronidase 1 bound to thiophenyl-beta-D-glucuronide [Bacteroides uniformis],6D7F_D Bacteroides uniformis beta-glucuronidase 1 bound to thiophenyl-beta-D-glucuronide [Bacteroides uniformis],6D7F_E Bacteroides uniformis beta-glucuronidase 1 bound to thiophenyl-beta-D-glucuronide [Bacteroides uniformis],6D7F_F Bacteroides uniformis beta-glucuronidase 1 bound to thiophenyl-beta-D-glucuronide [Bacteroides uniformis]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
T2KPJ7	9.06e-20	99	375	106	388	Putative beta-glucuronidase OS=Formosa agariphila (strain DSM 15362 / KCTC 12365 / LMG 23005 / KMM 3901 / M-2Alg 35-1) OX=1347342 GN=BN863_21970 PE=2 SV=1
T2KM09	1.31e-18	99	472	109	459	Putative beta-glucuronidase OS=Formosa agariphila (strain DSM 15362 / KCTC 12365 / LMG 23005 / KMM 3901 / M-2Alg 35-1) OX=1347342 GN=BN863_22050 PE=2 SV=2
P24131	2.09e-18	100	370	125	397	Beta-galactosidase OS=Clostridium acetobutylicum OX=1488 GN=cbgA PE=2 SV=2
P23989	2.27e-18	97	372	121	398	Beta-galactosidase OS=Streptococcus thermophilus OX=1308 GN=lacZ PE=3 SV=1
P81650	2.29e-18	51	372	49	408	Beta-galactosidase OS=Pseudoalteromonas haloplanktis OX=228 GN=lacZ PE=1 SV=2

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000093	0.000000	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

There is no transmembrane helices in MGYG000002173_00648.