CAZyme Information: MGYG000001859_01319

Basic Information

• Lineage: Bacteria; Firmicutes_C; Negativicutes; Acidaminococcales; Acidaminococcaceae; Phascolarctobacterium_A
• CAZyme ID: MGYG000001859_01319
• CAZy Family: GT83
• CAZyme Description: Undecaprenyl phosphate-alpha-4-amino-4-deoxy-L-arabinose arabinosyl transferase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
519	MGYG000001859_31|CGC1	59185.45	9.3389

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000001859	2029434	MAG	Denmark	Europe

• Gene Location: Start: 2950; End: 4509 Strand: +

Full Sequence      

MKQLEKNSLLLIAGLTLFTIMFGLGGVPLLDPDEPVYAETAREMLQTGDFLSPRIFGAYW
YDKPPMYYWLVALSQMLFGFSEFAARFPAALMACFTSIMIYVSTTKLFNERAGFWSAIIL
TSCVQFFYMGKAAVTDTTLLFFMTGAILSFAHKKYWLMYVCMALATVTKGPIGIVFPGAI
IFIYLVIMGQLREILRMHVIRGLLLYFLLASPWYYAMYTVHGMEFIETFLGFHNLTRFTT
PEHANRVTFWYYFPGVILGMFPWTGLLAQSIKASISDSRIDDMRQLLLFHVWWGFVFIFF
TICQTKLVSYILPMFPALAVIIGWNISRMLAKLRHNTTFYSWMAGSGLMFALLGVGWIFG
SQYLPEASFSLVMLGAMTLIMGAAAVFTLWYYRDIQMAAWLHALIGVVTMCVAFAFVLPV
IAERFSVKTMSGVYREQCDGSVPIYVDKFLRPGFMYYAGKPGIEMLPKTGALAEALRSGQ
HKYILVRGLEYRRVLKTKQIPTNVHTIKEISDIYLLEQR

Enzyme Prediction     

No EC number prediction in MGYG000001859_01319.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GT83	4	495	2.9e-115	0.9351851851851852

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
COG1807	ArnT	1.48e-59	9	391	11	401	4-amino-4-deoxy-L-arabinose transferase or related glycosyltransferase of PMT family [Cell wall/membrane/envelope biogenesis].
PRK13279	arnT	5.11e-38	2	334	1	341	lipid IV(A) 4-amino-4-deoxy-L-arabinosyltransferase.
pfam13231	PMT_2	2.78e-18	62	215	1	159	Dolichyl-phosphate-mannose-protein mannosyltransferase. This family contains members that are not captured by pfam02366.
pfam02366	PMT	5.00e-08	37	226	35	245	Dolichyl-phosphate-mannose-protein mannosyltransferase. This is a family of Dolichyl-phosphate-mannose-protein mannosyltransferase proteins EC:2.4.1.109. These proteins are responsible for O-linked glycosylation of proteins, they catalyze the reaction:- Dolichyl phosphate D-mannose + protein <=> dolichyl phosphate + O-D-mannosyl-protein. Also in this family is Drosophila rotated abdomen protein which is a putative mannosyltransferase. This family appears to be distantly related to pfam02516 (A Bateman pers. obs.). This family also contains sequences from ArnTs (4-amino-4-deoxy-L-arabinose lipid A transferase). They catalyze the addition of 4-amino-4-deoxy-l-arabinose (l-Ara4N) to the lipid A moiety of the lipopolysaccharide. This is a critical modification enabling bacteria (e.g. Escherichia coli and Salmonella typhimurium) to resist killing by antimicrobial peptides such as polymyxins. Members such as undecaprenyl phosphate-alpha-4-amino-4-deoxy-L-arabinose arabinosyl transferase are predicted to have 12 trans-membrane regions. The N-terminal portion of these proteins is hypothesized to have a conserved glycosylation activity which is shared between distantly related oligosaccharyltransferases ArnT and PglB families.
COG1287	Stt3	3.32e-04	62	210	86	255	Asparagine N-glycosylation enzyme, membrane subunit Stt3 [Posttranslational modification, protein turnover, chaperones].

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QTV77242.1	0.0	1	518	1	518
QNH53437.1	4.92e-167	2	495	5	503
ANR70824.1	5.62e-166	2	496	5	504
AOH48432.1	2.59e-164	2	496	5	504
AME03144.1	2.02e-158	2	496	5	504

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
5EZM_A	9.83e-34	29	329	54	367	CrystalStructure of ArnT from Cupriavidus metallidurans in the apo state [Cupriavidus metallidurans CH34],5F15_A Crystal Structure of ArnT from Cupriavidus metallidurans bound to Undecaprenyl phosphate [Cupriavidus metallidurans CH34]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
O67270	7.13e-41	3	342	1	338	Uncharacterized protein aq_1220 OS=Aquifex aeolicus (strain VF5) OX=224324 GN=aq_1220 PE=3 SV=1
O67601	7.93e-40	6	367	2	342	Uncharacterized protein aq_1704 OS=Aquifex aeolicus (strain VF5) OX=224324 GN=aq_1704 PE=3 SV=1
A0KGY4	4.54e-36	17	325	13	329	Undecaprenyl phosphate-alpha-4-amino-4-deoxy-L-arabinose arabinosyl transferase OS=Aeromonas hydrophila subsp. hydrophila (strain ATCC 7966 / DSM 30187 / BCRC 13018 / CCUG 14551 / JCM 1027 / KCTC 2358 / NCIMB 9240 / NCTC 8049) OX=380703 GN=arnT PE=3 SV=1
Q6D2F3	4.38e-35	2	372	3	387	Undecaprenyl phosphate-alpha-4-amino-4-deoxy-L-arabinose arabinosyl transferase OS=Pectobacterium atrosepticum (strain SCRI 1043 / ATCC BAA-672) OX=218491 GN=arnT PE=3 SV=1
A4SQX1	7.41e-35	28	323	24	327	Undecaprenyl phosphate-alpha-4-amino-4-deoxy-L-arabinose arabinosyl transferase OS=Aeromonas salmonicida (strain A449) OX=382245 GN=arnT PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.999980	0.000024	0.000004	0.000000	0.000000	0.000010

TMHMM  Annotations     

start	end
7	29
83	105
126	148
163	185
198	220
247	266
286	303
307	326
338	360
370	392
399	421