CAZyme Information: MGYG000001810_02451

Basic Information

• Species: Mediterraneibacter sp002314255
• Lineage: Bacteria; Firmicutes_A; Clostridia; Lachnospirales; Lachnospiraceae; Mediterraneibacter; Mediterraneibacter sp002314255
• CAZyme ID: MGYG000001810_02451
• CAZy Family: GH2
• CAZyme Description: Beta-galactosidase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
1603		176694.42	4.0197

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000001810	2943094	MAG	Denmark	Europe

• Gene Location: Start: 14083; End: 18894 Strand: +

Full Sequence      

MKRKWKKLLAGVCAAAMGLTMFSTAGIPAQSVQAEEAPSGREVLNFNSDWGFYRGDLENA
QDPDFDDSEFASVTLPHTMQLAKKHCPGANGVYQGVGWYRRYFTLDESYADKKIQLQFEG
VMTDSDVYLNGEKIYTRNGGYVGFTVDITDKVKFGENNVLALRVSKVDNPETPPGKPEAS
LDFHYWGGIYRDVNMIVTEKTSVTDVLEADQTAGGGVFLTYPEVSSEEATVNVKTDVAND
NTESHEIYVKQILEDEEGQAVIEQQSETQAVEAGGSYQFSQDLVVSDPHLWGIDDPYLYN
LVTEVYEDGVKVDEVENNAGIRTIDFKADGFYLNGERVYLRGANRHQAYQNVGDAAPDSM
QYRDALQIKQNGFNAVRAAHYPQDPAFLDACDELGIVVIECQPGWQNFTNTQTFYDRTIR
DTREMIRRDRNHPSVILWEASLNETNTSDQWAKDAVAAAREEYPGDQLFLASDYGYHGEL
YDVCYKVQDTQWSDNQDDWVDFDPEKPFLTREWGDWEGSSKALRKDGEDALNTQVYTRER
YLNGDGYSDWGGLDASERIGGYFLWSWNDYARGSNAVTLGSGTVDIDRYEKNGYYWLQSM
RPSDNPVYGPMVYISTDYTEESSLTVPVFSNCDSVRLYQNGELVQEITREEAGQDVPNIM
AKGGSPIYDFQLSEFAAGTLTAEGIVDGEVVCTHEVNTPGEAAGLQIEIADRGIEPVADG
SDLIPVYIKVVDDNGTVVPDYEGKVHVEVSGCGSLVGENIPRIQVQDQSPEKGIGFAFVR
TSMEAGEIQITAQAEGLADGVGTVTTAVSDDTFVPDGDHTEWTKTEADLEDPESNYKNIA
YGKEVTVSTEQSGNTKEKLVDDDDTSRWCAADGSFPQWAEVDLGEASALNGFQLLWENGA
AVYQYKIEVSNDRINYETVVDMSDNTQVNGYTETQLQKASGRYVRLTVTGCSSGWASLYE
FKVIPDEEAETPEPGELIPDSAVDRIEATSSAEDRGPEKLRDGVTEIGSGWLSESREFPQ
TVTVYFAKPQTLLGSRIYWEKDSSWYTYDLEVTKDGSTWEPVIRDLRVGGQHYKPEAFEE
IQENVVAARVTIKNVEAGGVYQIGMAEWMLYGYEYEAPDPEPVKEYEYLSDLEWISAHSD
YTDVKKDQAAYGGGLVLNTAEGRRDFAKGLGADTNSEIVYTLEPGKYDRFESYIGINANA
SKNGGEAIFRVYGDDELLYESPVMMRDDNCEFISLDIAGVEVLKLSAVWSENTENPEARY
NTHVDWADAKVYTHKEDTEEISTTVLEYALSLAETADTEGVVDSVLNNFNNARTAAEDIL
ARAQAGDPSLTQEMVDISWQNLIKAMQHLSFKQGDKTDLQKVVDMAKTLDLSEYLDEGQQ
VFADALAAAEAVLKDGDAMQDEVDQSWRGLLKAMSELRLKPNKDALKDLINEANGMSTEG
ADEETIAVFQNALAAAMSVYDDEQATEEEVMTAEEGLQAALDQLHAAVGDTEDPDNSGDE
GNTGSGGNDQWQSGQDQNIQDQSGKDQNGQIVASTGDNGTDISGRAAAGKVSSAGLAGSA
ENSSAQKSVKTGDTAAPIVGAAAAMTLAAAAGVIVYRRRRETR

Enzyme Prediction     

No EC number prediction in MGYG000001810_02451.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH2	33	598	7e-107	0.6170212765957447
CBM51	1128	1271	1.8e-31	0.9850746268656716
CBM32	847	962	1.3e-24	0.9112903225806451

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
COG3250	LacZ	5.98e-64	33	572	3	504	Beta-galactosidase/beta-glucuronidase [Carbohydrate transport and metabolism].
PRK10340	ebgA	9.11e-47	97	466	111	477	cryptic beta-D-galactosidase subunit alpha; Reviewed
PRK10150	PRK10150	1.61e-43	93	461	63	438	beta-D-glucuronidase; Provisional
PRK09525	lacZ	2.38e-36	96	444	121	462	beta-galactosidase.
pfam08305	NPCBM	4.82e-33	1126	1271	2	135	NPCBM/NEW2 domain. This novel putative carbohydrate binding module (NPCBM) domain is found at the N-terminus of glycosyl hydrolase family 98 proteins. This domain has also been called the NEW2 domain (Naumoff DG. Phylogenetic analysis of alpha-galactosidases of the GH27 family. Molecular Biology (Engl Transl). (2004)38:388-399.)

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
ANN35851.1	0.0	39	1274	16	1250
AXE81952.1	4.11e-318	11	1078	51	1183
QIH35625.1	1.08e-258	34	950	22	958
QUT49244.1	2.56e-252	39	963	27	969
CUA17213.1	5.38e-247	41	963	28	968

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
4YPJ_A	4.78e-79	40	806	6	807	ChainA, Beta galactosidase [Niallia circulans],4YPJ_B Chain B, Beta galactosidase [Niallia circulans]
7CWD_A	1.52e-76	44	811	4	806	ChainA, beta-glalactosidase [Niallia circulans],7CWI_A Chain A, beta-galactosidase [Niallia circulans]
6HPD_A	8.18e-69	39	799	33	797	Thestructure of a beta-glucuronidase from glycoside hydrolase family 2 [Formosa agariphila KMM 3901]
6ED1_A	3.05e-66	39	659	22	663	ChainA, Glycosyl hydrolase family 2, sugar binding domain protein [Phocaeicola dorei],6ED1_B Chain B, Glycosyl hydrolase family 2, sugar binding domain protein [Phocaeicola dorei],6ED1_C Chain C, Glycosyl hydrolase family 2, sugar binding domain protein [Phocaeicola dorei],6ED1_D Chain D, Glycosyl hydrolase family 2, sugar binding domain protein [Phocaeicola dorei]
5T98_A	5.93e-66	44	804	28	818	Crystalstructure of BuGH2Awt [Bacteroides uniformis],5T98_B Crystal structure of BuGH2Awt [Bacteroides uniformis],5T99_A Crystal structure of BuGH2Awt in complex with Galactoisofagomine [Bacteroides uniformis],5T99_B Crystal structure of BuGH2Awt in complex with Galactoisofagomine [Bacteroides uniformis]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
P77989	1.53e-93	40	799	2	730	Beta-galactosidase OS=Thermoanaerobacter pseudethanolicus (strain ATCC 33223 / 39E) OX=340099 GN=lacZ PE=3 SV=2
T2KN75	4.05e-68	39	799	24	788	Beta-glucuronidase OS=Formosa agariphila (strain DSM 15362 / KCTC 12365 / LMG 23005 / KMM 3901 / M-2Alg 35-1) OX=1347342 GN=BN863_22060 PE=1 SV=1
A7LXS9	1.39e-67	39	801	40	843	Beta-galactosidase BoGH2A OS=Bacteroides ovatus (strain ATCC 8483 / DSM 1896 / JCM 5824 / BCRC 10623 / CCUG 4943 / NCTC 11153) OX=411476 GN=BACOVA_02645 PE=1 SV=1
P26257	2.17e-63	41	759	2	708	Beta-galactosidase OS=Thermoanaerobacterium thermosulfurigenes OX=33950 GN=lacZ PE=1 SV=1
T2KPJ7	1.36e-58	34	761	44	781	Putative beta-glucuronidase OS=Formosa agariphila (strain DSM 15362 / KCTC 12365 / LMG 23005 / KMM 3901 / M-2Alg 35-1) OX=1347342 GN=BN863_21970 PE=2 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.000370	0.998842	0.000240	0.000222	0.000171	0.000145

TMHMM  Annotations     

start	end
7	29
1574	1596