CAZyme Information: MGYG000001761_02213

Basic Information

• Lineage: Bacteria; Firmicutes_A; Clostridia; Oscillospirales; Ruminococcaceae; Gemmiger
• CAZyme ID: MGYG000001761_02213
• CAZy Family: GH1
• CAZyme Description: 6-phospho-beta-galactosidase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
274		30058.25	5.8984

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000001761	2495136	MAG	Finland	Europe

• Gene Location: Start: 1265; End: 2089 Strand: -

Full Sequence      

MGLQLAAISKRFRLMAEQAARNAAAAGKSAEGTVQVGMNFQKMMENMKYAAAENAGVFGD
PQPKIFVSERTPEGDLLVMRAHTAAREAIRAICPHVKVGLTLSLHDLQAQPGGEPFAEAA
WKEEFTHYLPYIQGDDFLGVQNYTRTLYGPTGQLPAPEGAELTQMDYEFYPQALEHVLRK
VAKDFHGDLIVTENGIATADDTRRAAFIEQALAGVQRCVADGLPVKGYFHWSLMDNFEWQ
KGFAMNFGLIAVDRTTMARTPKPSLAVLGSYAGE

Enzyme Prediction     

EC	3.2.1.21

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH1	52	268	8.8e-46	0.5314685314685315

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
pfam00232	Glyco_hydro_1	2.90e-29	78	264	196	440	Glycosyl hydrolase family 1.
COG2723	BglB	9.13e-28	81	264	202	442	Beta-glucosidase/6-phospho-beta-glucosidase/beta-galactosidase [Carbohydrate transport and metabolism].
PRK13511	PRK13511	1.13e-22	136	264	292	455	6-phospho-beta-galactosidase; Provisional
PLN02814	PLN02814	2.55e-10	136	266	320	472	beta-glucosidase
PLN02998	PLN02998	1.07e-09	136	264	326	477	beta-glucosidase

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
AXB29242.1	2.99e-171	1	271	160	430
CBL02685.1	2.83e-169	1	273	160	432
AHF24559.1	1.36e-146	1	270	159	435
QTE67958.1	2.39e-146	1	271	159	425
AEN97245.1	5.40e-126	1	271	159	432

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
4R27_A	1.89e-59	50	272	188	409	Crystalstructure of beta-glycosidase BGL167 [Microbacterium sp. Gsoil167],4R27_B Crystal structure of beta-glycosidase BGL167 [Microbacterium sp. Gsoil167]
6IER_A	4.25e-52	77	272	235	428	Apostructure of a beta-glucosidase 1317 [uncultured bacterium]
1VFF_A	2.49e-27	116	272	236	403	beta-glycosidasefrom Pyrococcus horikoshii [Pyrococcus horikoshii]
6Z1H_A	4.10e-25	78	264	202	437	ChainA, ANCESTRAL RECONSTRUCTED GLYCOSIDASE [synthetic construct],6Z1H_B Chain B, ANCESTRAL RECONSTRUCTED GLYCOSIDASE [synthetic construct],6Z1M_A Chain A, Ancestral reconstructed glycosidase [synthetic construct],6Z1M_B Chain B, Ancestral reconstructed glycosidase [synthetic construct],6Z1M_C Chain C, Ancestral reconstructed glycosidase [synthetic construct]
3WDP_P	8.47e-25	63	272	225	452	Structuralanalysis of a beta-glucosidase mutant derived from a hyperthermophilic tetrameric structure [Pyrococcus furiosus],3WDP_Q Structural analysis of a beta-glucosidase mutant derived from a hyperthermophilic tetrameric structure [Pyrococcus furiosus],3WDP_R Structural analysis of a beta-glucosidase mutant derived from a hyperthermophilic tetrameric structure [Pyrococcus furiosus],3WDP_S Structural analysis of a beta-glucosidase mutant derived from a hyperthermophilic tetrameric structure [Pyrococcus furiosus]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
Q03506	9.23e-23	121	264	266	435	Beta-glucosidase OS=Niallia circulans OX=1397 GN=bglA PE=1 SV=3
P22505	2.85e-21	112	264	258	435	Beta-glucosidase B OS=Paenibacillus polymyxa OX=1406 GN=bglB PE=1 SV=1
P22073	5.31e-21	101	264	242	431	Beta-glucosidase A OS=Paenibacillus polymyxa OX=1406 GN=bglA PE=1 SV=1
P22498	4.08e-20	136	272	315	466	Beta-galactosidase OS=Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2) OX=273057 GN=lacS PE=1 SV=2
P50388	7.54e-20	136	272	315	466	Beta-galactosidase OS=Saccharolobus shibatae OX=2286 GN=bglY PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000053	0.000000	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

There is no transmembrane helices in MGYG000001761_02213.