CAZyme Information: MGYG000001737_00746

Basic Information

• Species: Eubacterium_R sp000434995
• Lineage: Bacteria; Firmicutes_A; Clostridia; Oscillospirales; Acutalibacteraceae; Eubacterium_R; Eubacterium_R sp000434995
• CAZyme ID: MGYG000001737_00746
• CAZy Family: GH2
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
849	MGYG000001737_2|CGC3	97824.27	5.8796

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000001737	1877486	MAG	Sweden	Europe

• Gene Location: Start: 218490; End: 221039 Strand: -

Full Sequence      

MIKTLNGAWLFKAVDENEWKNATVPGCNFLDLMNNGDIPNPFISTYEKEVGFVGEKDWEY
KRIFEISEAELDCDDVLLNCKMLDTICDVYVNSRLLFAGDNCFVAYSKSVKSFLNIGENE
IRIVFKSPVNYVRDKYKKCPTPINSNGQNGIVHIRKPQSHFGWDWGPVLPLSGITKDIEL
QFVNNAKIDYLGITQRLENGKAFVSVKADVTSFGNYECEIGITSPSGEVMTDCGECAEFV
IDNPELWWTYELSGSKNQPLYTVSAKLKLNGKVLDEQSKKIGLRTIELNRERDKYGRNFQ
FILNGVPLFIKGSNYIPADSFITRFDKQKLDYMLDAVQFSNMNMIRVWGGGYYESDEFYD
ACDERGILVWQDFQFACQAYPFFDDAFLENVKREVEYNVKRLCNHPSLAVWNGNNEIEDM
HSAWMYMTKYVEWTEKFFYHILEAEIRKYDKSTPYTPTSPVGEKHNYGVGSDNVGDTHLW
AVWHGLKPMNYYRKRMTRFCSEFGFESLPDMKSIDIFAEHKGNYSLNDKVFNAHQKCANG
NDKMVYYVASRFNLPKNFKDMVYLSQVTQNECIADATEHWRRNKGRCNGSMYWQLNDCWG
VCSWSSIDYYGNYKALQYGARHFNAPVSVSIENTEEYIKLFVLNDLKEKQDVTVTYDIFD
FVSGTKQSDKKDVSIDAVKNELVFELDVKSLAKAYDLKRTGVAVKLFKDGELLQQKTVLF
DKEKKLDLPKAKLKTRIEIKEDRLDITVKSDAYARLVKLESADSVLPFSDNFFDILPNES
KKVTIYKDSSMSLRKLAESITVYSLCDIKFSKDKIDTLLKKAKVFISPVNISNALYHGKI
AKDVELCDD

Enzyme Prediction     

No EC number prediction in MGYG000001737_00746.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH2	3	748	2.3e-100	0.7659574468085106

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
COG3250	LacZ	1.24e-77	1	630	11	635	Beta-galactosidase/beta-glucuronidase [Carbohydrate transport and metabolism].
pfam17753	Ig_mannosidase	6.59e-21	730	807	1	78	Ig-fold domain. This Ig-like fold domain is found in mannosidase enzymes.
PRK10340	ebgA	7.04e-13	3	416	42	449	cryptic beta-D-galactosidase subunit alpha; Reviewed
pfam00703	Glyco_hydro_2	3.49e-10	186	284	1	106	Glycosyl hydrolases family 2. This family contains beta-galactosidase, beta-mannosidase and beta-glucuronidase activities.
pfam17786	Mannosidase_ig	1.91e-09	638	724	3	90	Mannosidase Ig/CBM-like domain. This domain corresponds to domain 4 in the structure of Bacteroides thetaiotaomicron beta-mannosidase, BtMan2A. This domain has an Ig-like fold.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
BCJ98156.1	5.64e-201	3	822	4	839
AUG59052.1	7.53e-198	1	807	1	816
AVQ95585.1	4.29e-185	5	799	6	808
ADU26456.1	4.29e-185	5	799	6	808
AYF38249.1	4.29e-185	5	799	6	808

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
2VJX_A	9.01e-154	2	785	8	816	Structuraland biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VJX_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VL4_A Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VL4_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VMF_A Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VMF_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VO5_A Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VO5_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VOT_A Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VOT_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VQT_A Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron],2VQT_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron],2VR4_A Transition-state mimicry in mannoside hydrolysis: characterisation of twenty six inhibitors and insight into binding from linear free energy relationships and 3-D structure [Bacteroides thetaiotaomicron VPI-5482],2VR4_B Transition-state mimicry in mannoside hydrolysis: characterisation of twenty six inhibitors and insight into binding from linear free energy relationships and 3-D structure [Bacteroides thetaiotaomicron VPI-5482]
2JE8_A	9.52e-154	2	785	10	818	Structureof a beta-mannosidase from Bacteroides thetaiotaomicron [Bacteroides thetaiotaomicron VPI-5482],2JE8_B Structure of a beta-mannosidase from Bacteroides thetaiotaomicron [Bacteroides thetaiotaomicron VPI-5482]
7OP6_A	9.79e-154	2	785	10	818	ChainA, Beta-mannosidase [Bacteroides thetaiotaomicron VPI-5482],7OP6_B Chain B, Beta-mannosidase [Bacteroides thetaiotaomicron VPI-5482],7OP7_A Chain A, Beta-mannosidase [Bacteroides thetaiotaomicron VPI-5482],7OP7_B Chain B, Beta-mannosidase [Bacteroides thetaiotaomicron VPI-5482]
2WBK_A	2.58e-153	2	785	8	816	Structureof the Michaelis complex of beta-mannosidase, Man2A, provides insight into the conformational itinerary of mannoside hydrolysis [Bacteroides thetaiotaomicron VPI-5482],2WBK_B Structure of the Michaelis complex of beta-mannosidase, Man2A, provides insight into the conformational itinerary of mannoside hydrolysis [Bacteroides thetaiotaomicron VPI-5482]
2VQU_A	1.07e-151	2	785	8	816	Structuraland biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron],2VQU_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
Q29444	1.52e-107	4	807	24	877	Beta-mannosidase OS=Bos taurus OX=9913 GN=MANBA PE=1 SV=1
Q5B7W2	5.06e-107	5	785	9	826	Beta-mannosidase B OS=Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) OX=227321 GN=mndB PE=1 SV=2
Q95327	3.14e-104	4	807	24	877	Beta-mannosidase OS=Capra hircus OX=9925 GN=MANBA PE=1 SV=1
A2QYN2	2.10e-103	4	785	8	828	Beta-mannosidase B OS=Aspergillus niger (strain CBS 513.88 / FGSC A1513) OX=425011 GN=mndB PE=3 SV=1
Q0CCA0	7.74e-103	3	785	7	826	Beta-mannosidase B OS=Aspergillus terreus (strain NIH 2624 / FGSC A1156) OX=341663 GN=mndB PE=3 SV=2

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000063	0.000000	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

There is no transmembrane helices in MGYG000001737_00746.