CAZyme Information: MGYG000001371_06370

Basic Information

• Species: Paenibacillus lautus_A
• Lineage: Bacteria; Firmicutes; Bacilli; Paenibacillales; Paenibacillaceae; Paenibacillus; Paenibacillus lautus_A
• CAZyme ID: MGYG000001371_06370
• CAZy Family: CBM48
• CAZyme Description: 1,4-alpha-glucan branching enzyme GlgB

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
668		76877.41	5.7477

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000001371	6950059	Isolate	not provided	not provided

• Gene Location: Start: 2978; End: 4984 Strand: -

Full Sequence      

MNQAVREGNMTPEQVYLFHEGTWFHSYQCMGAHLAAEEGEEGVRFTVWAPNARQVGLAGD
WNGWDGSQDPLYRMPDSGIWSRFFPGMETGTFYKYQITGPFGETFLKADPYAFHAEVRPA
TASVVTDLSGYSWNDAAWRRNNKSPYAKPVNIYEMHFGTWRQKEDGSFFTYRELADQLIP
YLQEMSYTHVEFMPLAEHPYDLSWGYQGTGFYALTSRYGTPHDFMYLVDQLHQAGIGVLL
DWVPAHFAKDAHGLRQFDGAPLYEYEDPQKAEKPGWGTLSFDYAKPEVISFLISNALFWM
DMYHIDGLRVDAVTSMIRLDFEKREGQYSLNEHGGLENLEAISFLQELNKAVFRYYPNAL
MMAEESSAWAGVTAPVHEGGLGFNYKWNMGWMNDTLSYIEQEFDQRPTHHNLLTFPICYA
YSENFTLPLSHDEVVHGKKSLLNKMPGSYEQKFSGLRILLGYQITQPGKNLLFMGGEFGQ
FIEWKDQEQLDWLLLDYESHRKQLAYTAALNRMYVDEKALWEQDHRWEGFEWLSADDHEQ
SVVSYMRMGTKPGDTLIIVINFQPKAYDQYRIGLPRAGQYVEILNSDHADYGGSGLLNDG
EMKAEKKTWHGQTHSLEIKLPPLGMVVLKKKPPRSRKSQDRSDASLKAAAAREKTEQKTK
RSKRGKQD

Enzyme Prediction     

EC	2.4.1.18

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH13	180	480	4.2e-147	0.9966777408637874
CBM48	30	115	5.2e-17	0.8947368421052632

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
PRK05402	PRK05402	0.0	10	632	100	725	1,4-alpha-glucan branching protein GlgB.
PRK14706	PRK14706	0.0	30	648	27	639	glycogen branching enzyme; Provisional
PRK14705	PRK14705	0.0	19	624	616	1216	glycogen branching enzyme; Provisional
PRK12313	PRK12313	0.0	11	632	8	629	1,4-alpha-glucan branching protein GlgB.
COG0296	GlgB	0.0	10	629	6	627	1,4-alpha-glucan branching enzyme [Carbohydrate transport and metabolism].

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
ACX64696.1	0.0	1	668	1	668
QOT13512.1	0.0	10	668	1	659
AYB44972.1	0.0	10	668	1	659
AWP28196.1	0.0	10	668	1	659
AVV59028.1	0.0	10	668	1	659

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
5GQW_A	1.42e-226	10	632	129	775	Crystalstructure of branching enzyme W610N mutant from Cyanothece sp. ATCC 51142 [Crocosphaera subtropica ATCC 51142],5GQX_A Crystal structure of branching enzyme W610N mutant from Cyanothece sp. ATCC 51142 in complex with maltoheptaose [Crocosphaera subtropica ATCC 51142]
5GR2_A	2.02e-226	10	632	129	775	Crystalstructure of branching enzyme L541A mutant from Cyanothece sp. ATCC 51142 [Crocosphaera subtropica ATCC 51142],5GR4_A Crystal structure of branching enzyme L541A mutant from Cyanothece sp. ATCC 51142 in complex with maltoheptaose [Crocosphaera subtropica ATCC 51142]
5GR5_A	2.02e-226	10	632	129	775	Crystalstructure of branching enzyme W610A mutant from Cyanothece sp. ATCC 51142 [Crocosphaera subtropica ATCC 51142]
5GQU_A	2.86e-226	10	632	129	775	Crystalstructure of branching enzyme from Cyanothece sp. ATCC 51142 [Crocosphaera subtropica ATCC 51142],5GQV_A Crystal structure of branching enzyme from Cyanothece sp. ATCC 51142 in complex with maltohexaose [Crocosphaera subtropica ATCC 51142],5GQY_A Crystal structure of branching enzyme from Cyanothece sp. ATCC 51142 in complex with maltoheptaose [Crocosphaera subtropica ATCC 51142]
5GR0_A	8.10e-226	10	632	129	775	Crystalstructure of branching enzyme D501A mutant from Cyanothece sp. ATCC 51142 [Crocosphaera subtropica ATCC 51142]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
Q24VW3	1.65e-268	10	629	12	628	1,4-alpha-glucan branching enzyme GlgB OS=Desulfitobacterium hafniense (strain Y51) OX=138119 GN=glgB PE=3 SV=1
B8CVY1	5.83e-266	16	629	12	626	1,4-alpha-glucan branching enzyme GlgB OS=Halothermothrix orenii (strain H 168 / OCM 544 / DSM 9562) OX=373903 GN=glgB PE=3 SV=1
P30537	3.98e-258	9	643	5	631	1,4-alpha-glucan branching enzyme GlgB OS=Bacillus caldolyticus OX=1394 GN=glgB PE=1 SV=1
Q8CZE8	1.65e-257	9	636	3	633	1,4-alpha-glucan branching enzyme GlgB OS=Oceanobacillus iheyensis (strain DSM 14371 / CIP 107618 / JCM 11309 / KCTC 3954 / HTE831) OX=221109 GN=glgB PE=3 SV=1
B9J2G8	2.90e-255	18	645	14	631	1,4-alpha-glucan branching enzyme GlgB OS=Bacillus cereus (strain Q1) OX=361100 GN=glgB PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000050	0.000000	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

There is no transmembrane helices in MGYG000001371_06370.