CAZyme Information: MGYG000001185_00880

Basic Information

• Species: CAG-269 sp900554565
• Lineage: Bacteria; Firmicutes_A; Clostridia; TANB77; CAG-508; CAG-269; CAG-269 sp900554565
• CAZyme ID: MGYG000001185_00880
• CAZy Family: GT84
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
2219		257194.26	9.0482

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000001185	1357656	MAG	Austria	Europe

• Gene Location: Start: 45058; End: 51717 Strand: -

Full Sequence      

MKTNKILKINGARLDQNQLENHLQKIASNHNLTSKSQKNTYPIPHMIESYQIIQEVYKLL
NEHLKLGISIHPAGEWLLDNFYIIEETAKQIEKELTLSKYTNFLGIANGQYKGFARIYVL
ASEIVAYTDNKIEQKALENYLASYQTKKTLSMEEIWNIGLFLEIAIIENIREICEKIYSA
QIQKYKAENIVERLVENKPKSELTFKNNQIRKIKKDILEEMEYPFIEYMSYILKRYGKKG
YSYLKILEETVELKGTTVSDVIKKEHFDIAVRKVSIGNSITSIKKIQRMNFLDIFEKVNG
VEELLKQDPSGIYEKMDNKTKEYYRNKIKEISKKTKISELYITRKILELAQQNPKSIKKS
HIGYYLIDAGTEELYKILNYKPRKNKKPQTKTKIYIATISFFTIIISTLLTCMLKIPNNY
VKIIAFILFIIPSSELTIQIIQYILSKIVKPKIIPKLDLYGGIEPQYQTMVVIPTIIKSK
EKVKELFEKLEVYYLANKSENLYFTLLGDCSESNHKEEEYDIEVIQEGKNQVQKLNEKYG
NIFNFLYREREYNPKEGTYLGWERKRGMLNQLNDYLLGNIKNPFRTNTIEENSKQKEILK
NTKYIITLDADTDLILDSAFELVGAMAHILNKPKIEGKIVVDGYGIMQPRVGVNLDVSYK
NLFTKIFAGAGGIDSYTNAISDIYQDNFKEGIFTGKGIYDLKVFSEVMKGAIPENTVLSH
DLLEGSYLRCGLVSDIMLMDGYPSKYNSFMNRLSRWTRGDWQITSWLGRNSPLNLLSKYK
IFDNLRRSLLEISIIVAIIFTNIIKIAYAKSISIVILILLAILILPFLLEIFNMIIFKKE
GEEKQKTFTPKISGFKGSILRALIILGTMPYKAYVLAKSIIKTIYRKHISKKHLLEWMTS
EEAEKQAKTSVISYYNQMAINVLVGICAIGIGLLEKSILAVMLGAYFIVIPFIMYYVSKE
KEKPEPIQKLDKQEQEYIKEIGKKTWNYFERYLNKENNYLIPDNYQEDRKEKTVPRTSST
NIGLSLLAIISAYDLKYIEKEKAEELLEKVITSIDSLPKWNGHLYNWYQTQTKQPLIPRY
VSTVDSGNFVGYLYVTKAFLRNEVRNENLVNIVNNIIEKTDFSVLYNWEQQIFSIGFSIE
ENKLTDSYYDLLASEARQASLVAIAKKDVPAKHWEHLSRSLTILGKYKGLISWSGTAFEY
LMPNINIPRYEGSLLDESCKFMIMSQKEYAKQLGIPWGISEAAFNVKDLKSNYQYKAFGI
PWLGLKRGLADELVVSSYGGILAILDDPKGEIENLKRLEKEGMYNKYGFYESIDYTPERV
EKGKTSSVVKTYMAHHQGLILLSINNLFNKKILQKRFIENPEIKAVSILLQETMPEKALI
TKENKEKIEKLKYKDYENYVVTTNNKIDDRLITGNVISNENYTIAMNEKGEGISKYKDIY
INRYKLTDDYSQGIFFAIKNIKTKKILSSNYKLNEKNNYQISFMPDKDKQEISGENIKTQ
IITTVSSNEPVELRRVILENIGQEEEILEVTSYFEPVLSKKEQEYAHPAFNNLFLISKFD
EEKNSVIVERKNREPNGRKMFLATTLSTNSETIGDLEYEINEEKFIGRGNLKTPQMVENL
VPLSKKIGLVLEPIVALKRTIKLKPKEEKIVDLIISVGEEESAVKENLEKYKSIENVKTE
IEISKARVEAESRYLRIKGKEIAIYQKILSYIIFDNSPKSITLSKNLQTSYKQSQLWKYG
ISGDLPIILVKIKDANDSYVIKEILKAHEFFRTKNVQTEVVILDEEKHSYENYVKEEIEN
DILNSQMAYLRNIKGGIFTLSKGEIDEKDIKLLELISVIKIDTSKGGIENTVKEIEEEYL
EKYKQIGEEDKPEVIEETDGDINILENIDKLKYYNEYGGFSEDGKEYLIKVNKKNRLPTV
WSHIMANKTFGTIVTENMGGYTWYKNSRLNRVSSWENNPSYDIPSEIIYLKDDETKKAWS
LGLNPMPDEKNYNIIYGFGYCKYIHKSDGIEQELEIFVPKEETCKINILTLKNTTPNRKK
LKLYYYIKPVIGEDEIKSNQYITVNLDKNSNIVWAKNLYQTEELENLIYISTSEKIKSYT
GDKNFFLGNEGLSNPQGLRKERLNNETGLGKKSCMVFEIEIQLESYAKKEITLILGADEK
IINCKNTAYKYSKIQNARQELEICKNAWKELLRKTTSIYPIRINKYIIKWLDTISNFSK

Enzyme Prediction     

EC	2.4.1.-

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH94	1597	2194	8.3e-126	0.5395752895752896
GT84	1147	1361	1.6e-90	0.9953488372093023

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
cd11756	GH94N_ChvB_NdvB_1_like	1.10e-84	1890	2159	1	271	First GH94N domain of cyclic beta 1-2 glucan synthetase and similar domains. The glycoside hydrolase family 94 (previously known as glycosyltransferase family 36) includes cyclic beta 1-2 glucan synthetase (EC:2.4.1.20) or ChvB (encoded by the chromosomal chvB virulence gene). This first of two tandemly repeated GH94-N-terminal-like domains has not been characterized functionally. Some beta 1-2 glucan synthetases are annotated as NdvB (nodule development B) gene products, glycosyltransferases required for the synthesis of cyclic beta-(1,2)-glucans, which play a role in interactions between bacteria and plants.
pfam06165	Glyco_transf_36	3.45e-79	1918	2156	1	247	Glycosyltransferase family 36. The glycosyltransferase family 36 includes cellobiose phosphorylase (EC:2.4.1.20), cellodextrin phosphorylase (EC:2.4.1.49), chitobiose phosphorylase (EC:2.4.1.-). Many members of this family contain two copies of this domain.
COG3459	COG3459	2.46e-70	1597	2192	1	569	Cellobiose phosphorylase [Carbohydrate transport and metabolism].
cd11753	GH94N_ChvB_NdvB_2_like	3.70e-63	1353	1677	1	330	Second GH94N domain of cyclic beta 1-2 glucan synthetase and similar domains. The glycoside hydrolase family 94 (previously known as glycosyltransferase family 36) includes cyclic beta 1-2 glucan synthetase (EC:2.4.1.20) or ChvB (encoded by the chromosomal chvB virulence gene). This second of two tandemly repeated GH94-N-terminal-like domains has not been characterized functionally. Some beta 1-2 glucan synthetases are annotated as NdvB (nodule development B) gene products, glycosyltransferases required for the synthesis of cyclic beta-(1,2)-glucans, which play a role in interactions between bacteria and plants.
pfam06165	Glyco_transf_36	1.32e-60	1415	1655	6	246	Glycosyltransferase family 36. The glycosyltransferase family 36 includes cellobiose phosphorylase (EC:2.4.1.20), cellodextrin phosphorylase (EC:2.4.1.49), chitobiose phosphorylase (EC:2.4.1.-). Many members of this family contain two copies of this domain.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
AUG57227.1	0.0	6	2211	31	2434
AEV69563.1	0.0	7	2211	32	2444
ADU74104.1	0.0	7	2188	36	2420
ANV75789.1	0.0	7	2188	36	2420
ABN52453.1	0.0	7	2188	36	2420

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
1V7V_A	2.60e-24	1897	2194	3	303	Crystalstructure of Vibrio proteolyticus chitobiose phosphorylase [Vibrio proteolyticus],1V7W_A Crystal structure of Vibrio proteolyticus chitobiose phosphorylase in complex with GlcNAc [Vibrio proteolyticus],1V7X_A Crystal structure of Vibrio proteolyticus chitobiose phosphorylase in complex with GlcNAc and sulfate [Vibrio proteolyticus]
3QDE_A	2.62e-24	1897	2210	3	331	ChainA, Cellobiose phosphorylase [Acetivibrio thermocellus],3QDE_B Chain B, Cellobiose phosphorylase [Acetivibrio thermocellus]
3RRS_A	2.46e-10	1897	2048	3	151	Crystalstructure analysis of cellobiose phosphorylase from Cellulomonas uda [Cellulomonas uda],3RRS_B Crystal structure analysis of cellobiose phosphorylase from Cellulomonas uda [Cellulomonas uda],3RSY_A Cellobiose phosphorylase from Cellulomonas uda in complex with sulfate and glycerol [Cellulomonas uda],3RSY_B Cellobiose phosphorylase from Cellulomonas uda in complex with sulfate and glycerol [Cellulomonas uda],3S4A_A Cellobiose phosphorylase from Cellulomonas uda in complex with cellobiose [Cellulomonas uda],3S4A_B Cellobiose phosphorylase from Cellulomonas uda in complex with cellobiose [Cellulomonas uda],3S4B_A Cellobiose phosphorylase from Cellulomonas uda in complex with glucose [Cellulomonas uda],3S4B_B Cellobiose phosphorylase from Cellulomonas uda in complex with glucose [Cellulomonas uda]
3S4C_A	2.46e-10	1897	2048	3	151	Lactosephosphorylase in complex with sulfate [Cellulomonas uda],3S4D_A Lactose phosphorylase in a ternary complex with cellobiose and sulfate [Cellulomonas uda]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
P20471	2.46e-262	48	2156	64	2314	Cyclic beta-(1,2)-glucan synthase NdvB OS=Rhizobium meliloti (strain 1021) OX=266834 GN=ndvB PE=1 SV=2
Q9F8X1	4.68e-24	1897	2194	3	303	N,N'-diacetylchitobiose phosphorylase OS=Vibrio furnissii OX=29494 GN=chbP PE=1 SV=1
Q76IQ9	1.41e-23	1897	2194	3	303	N,N'-diacetylchitobiose phosphorylase OS=Vibrio proteolyticus OX=671 GN=chbP PE=1 SV=1
B9K7M6	1.07e-18	1897	2210	3	332	Cellobiose phosphorylase OS=Thermotoga neapolitana (strain ATCC 49049 / DSM 4359 / NBRC 107923 / NS-E) OX=309803 GN=cbpA PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.999984	0.000039	0.000001	0.000000	0.000000	0.000000

TMHMM  Annotations     

start	end
394	416
423	445
789	808
815	837
857	876
922	944