CAZyme Information: MGYG000001106_00584

Basic Information

• Lineage: Bacteria; Firmicutes_A; Clostridia; Oscillospirales; Ruminococcaceae; Pygmaiobacter
• CAZyme ID: MGYG000001106_00584
• CAZy Family: GT51
• CAZyme Description: Penicillin-binding protein 1F

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
725		78751.98	4.4818

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000001106	1936587	MAG	China	Asia

• Gene Location: Start: 1463; End: 3640 Strand: -

Full Sequence      

MWANIGRVLVVLVSIGVIVASVLAVMLSLYVVKVTANDATLLSLENLKLSYSSTIYAINA
QTGEPEPYQRLVDNADRIWVDGNQIPDNLKNAFIAVEDKTFRENNGVNFKRTVSATANLV
LSKLTGGKIKLYDTEQGASTITQQLIKNITGDDDHDALRKVREIFRAISLTGRYSKDTIL
EAYLNTVGFMGNTCGVQACAYNLYDKDVSQLTLAQCATVAAITNNPTKYNPRTEPENNLQ
RRNMILGMMLEQGLITQAEHDAAVAEPLNLIDPAEKQNTAKVGNNSYFTDMVIDEVITDL
VSQGICETRSEASALLYNGGLAIYSTVDTNLQSIMEEVMAKEPGNYYPDQEVEYEDKATG
ETKTQNVQGAMITIDYTGAVKGVVGGLGEKTADRGLNRAVDSLRQTGSTMKPIGAYAMGI
DYNYITYSDGGFIDDWVEEIEDKGSMRKWPVNYSGRYTEQRMLVCDALANSINTIAVRVG
KLVGPDDMFDFMTNTLGITSLVESRTEADGKISTDIGVGPLVLGSMTDGVSLAEMAHAYM
MFGDGGTVTPLHSYTTVENVQTGEVVLDNTLLPTTRAIGEDTAMIMNKLLRQVITRGTAA
GTGTNTIAGMETVGKTGTTSNDKDHWFIGLTPYYVTATWMGYDQPQELAWKAYGKHPPTL
AWKEVMERAQEGLEAKNFPTAPAGTVVTQAYCTESGGAAGPLCPKTATGYYKADQLPEPC
DIHVG

Enzyme Prediction     

No EC number prediction in MGYG000001106_00584.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GT51	73	249	7.3e-54	0.943502824858757

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
COG0744	MrcB	4.53e-149	5	701	11	628	Membrane carboxypeptidase (penicillin-binding protein) [Cell wall/membrane/envelope biogenesis].
TIGR02074	PBP_1a_fam	4.97e-142	76	672	1	531	penicillin-binding protein, 1A family. Bacterial that synthesize a cell wall of peptidoglycan (murein) generally have several transglycosylases and transpeptidases for the task. This family consists of bifunctional transglycosylase/transpeptidase penicillin-binding proteins (PBP). In the Proteobacteria, this family includes PBP 1A but not the paralogous PBP 1B (TIGR02071). This family also includes related proteins, often designated PBP 1A, from other bacterial lineages. [Cell envelope, Biosynthesis and degradation of murein sacculus and peptidoglycan]
COG5009	MrcA	1.30e-102	5	724	5	772	Membrane carboxypeptidase/penicillin-binding protein [Cell wall/membrane/envelope biogenesis].
TIGR02071	PBP_1b	1.42e-77	76	692	146	712	penicillin-binding protein 1B. Bacterial that synthesize a cell wall of peptidoglycan (murein) generally have several transglycosylases and transpeptidases for the task. This family consists of a particular bifunctional transglycosylase/transpeptidase in E. coli and other Proteobacteria, designated penicillin-binding protein 1B. [Cell envelope, Biosynthesis and degradation of murein sacculus and peptidoglycan]
pfam00912	Transgly	4.92e-60	77	250	13	177	Transglycosylase. The penicillin-binding proteins are bifunctional proteins consisting of transglycosylase and transpeptidase in the N- and C-terminus respectively. The transglycosylase domain catalyzes the polymerization of murein glycan chains.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
CBL02306.1	3.49e-172	5	720	46	796
ATL89188.1	8.64e-172	6	721	37	787
AXB28952.1	1.52e-171	5	720	61	811
ATO98888.1	2.43e-171	6	721	37	787
QIA44319.1	4.01e-171	6	721	54	804

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
3UDF_A	1.80e-46	78	675	40	721	ChainA, Penicillin-binding protein 1a [Acinetobacter baumannii],3UDF_B Chain B, Penicillin-binding protein 1a [Acinetobacter baumannii],3UDI_A Chain A, Penicillin-binding protein 1a [Acinetobacter baumannii],3UDI_B Chain B, Penicillin-binding protein 1a [Acinetobacter baumannii],3UDX_A Chain A, Penicillin-binding protein 1a [Acinetobacter baumannii],3UDX_B Chain B, Penicillin-binding protein 1a [Acinetobacter baumannii],3UE0_A Chain A, Penicillin-binding protein 1a [Acinetobacter baumannii],3UE0_B Chain B, Penicillin-binding protein 1a [Acinetobacter baumannii],3UE1_A Chain A, Penicillin-binding protein 1a [Acinetobacter baumannii],3UE1_B Chain B, Penicillin-binding protein 1a [Acinetobacter baumannii]
3ZG8_B	6.44e-44	174	644	7	434	CrystalStructure of Penicillin Binding Protein 4 from Listeria monocytogenes in the Ampicillin bound form [Listeria monocytogenes],3ZG9_B Crystal Structure of Penicillin-Binding Protein 4 from Listeria monocytogenes in the Cefuroxime bound form [Listeria monocytogenes],3ZGA_B Crystal Structure of Penicillin-Binding Protein 4 from Listeria monocytogenes in the Carbenicillin bound form [Listeria monocytogenes]
5FGZ_A	2.73e-43	77	654	156	678	E.coli PBP1b in complex with FPI-1465 [Escherichia coli K-12],5HL9_A E. coli PBP1b in complex with acyl-ampicillin and moenomycin [Escherichia coli K-12],5HLA_A E. coli PBP1b in complex with acyl-cephalexin and moenomycin [Escherichia coli K-12],5HLB_A E. coli PBP1b in complex with acyl-aztreonam and moenomycin [Escherichia coli K-12],5HLD_A E. coli PBP1b in complex with acyl-CENTA and moenomycin [Escherichia coli K-12],6YN0_A Structure of E. coli PBP1b with a FtsN peptide activating transglycosylase activity [Escherichia coli K-12],7LQ6_A Chain A, Penicillin-binding protein 1B [Escherichia coli K-12]
3VMA_A	3.15e-43	77	654	177	699	CrystalStructure of the Full-Length Transglycosylase PBP1b from Escherichia coli [Escherichia coli K-12]
7U4H_A	1.11e-41	69	678	32	769	ChainA, Penicillin-binding protein 1A (Pbp1a) [Chlamydia trachomatis D/UW-3/CX],7U4H_B Chain B, Penicillin-binding protein 1A (Pbp1a) [Chlamydia trachomatis D/UW-3/CX]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
Q0SRL7	3.45e-72	71	711	85	708	Penicillin-binding protein 1A OS=Clostridium perfringens (strain SM101 / Type A) OX=289380 GN=pbpA PE=3 SV=1
Q0TNZ8	2.00e-71	71	711	85	708	Penicillin-binding protein 1A OS=Clostridium perfringens (strain ATCC 13124 / DSM 756 / JCM 1290 / NCIMB 6125 / NCTC 8237 / Type A) OX=195103 GN=pbpA PE=3 SV=1
Q8XJ01	7.36e-71	71	648	85	646	Penicillin-binding protein 1A OS=Clostridium perfringens (strain 13 / Type A) OX=195102 GN=pbpA PE=3 SV=1
A7GHV1	2.41e-65	70	676	69	650	Penicillin-binding protein 1A OS=Clostridium botulinum (strain Langeland / NCTC 10281 / Type F) OX=441772 GN=pbpA PE=3 SV=1
Q891X1	2.75e-65	71	725	74	715	Penicillin-binding protein 1A OS=Clostridium tetani (strain Massachusetts / E88) OX=212717 GN=pbpA PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.957581	0.036620	0.000377	0.000330	0.000160	0.004964

TMHMM  Annotations     

start	end
9	31