CAZyme Information: MGYG000001027_00441

Basic Information

• Species: CAG-56 sp900752065
• Lineage: Bacteria; Firmicutes_A; Clostridia; Lachnospirales; Lachnospiraceae; CAG-56; CAG-56 sp900752065
• CAZyme ID: MGYG000001027_00441
• CAZy Family: GH101
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
2216	MGYG000001027_35|CGC1	238495.48	4.8461

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000001027	2760146	MAG	Sweden	Europe

• Gene Location: Start: 5516; End: 12166 Strand: +

Full Sequence      

MKEKGNKQRLRSFMAALLALVLAFSPVLSSIPAIQAEAADAKIWYHVETGSGNGNAHTYS
DASTSPAAVLLHRTATMPADGTFSVTYEKQGDNTASARLGFFYTYVDDSNFLYVGFDTQS
HWFYEYKVNGGGSYSTISGLPDQTDGSRTDFSISVSREALTVKVNDTIKSVNVQDFVSLM
EKAGGNGRFGFRGGNPTSFHFTDAKVNNEAVSNEWGFLADCAGQTFQEEEPVPVYTVTGT
VKNTANQPIAGANVRIGYSSTKTDAAGSFSIDVEEGTYDVTVSAAGYAAQTVAGVEINSN
KSLEAMSLVEKAAVVYKNYIQKDSIKAAVSEKFPQVMQYKLNVDGNEKVFAGQEDELSVI
KINGVAITPVMGDVQINADNAVYPMTLKKDSIDMEMTVKISVKENDLTWEVTDITKKEGC
AKIHTIEVPDLNLVTISDEQKDAQFMGANVSGNVQVSGDEKITFDEGFSANSSAGYAYGF
LSADGISAGVWSNSEMAGDKRLVRNNSANSMSLTSALWYYEYGDLAASATLDGTPVSELP
STKVCLAGDENRDGTVDWQDGAIAYRDIMNNPYGSENTKDLVNYRISMNFSSQATNPYLK
TADNIKKVYLATDGLPQAVMMKGYGSEGHDSANSEYGKIADRLGGLEELKKLNSIAHQYH
TQMGIHINAQEVYPEAQSFTNELINGPSALGWGWLDQSYTINRPYDLASGLRYKRLFQLY
DQLNGTSLYANKWPGVVGTGTDETVADAETIAETVAEKKLTTDENLDFMYLDVWYGDSWE
TRKIAQQINSLGWRFSTEFGYEGEYDSTWQHWATEGHYGGSSMKGLNSDVIRFIRNHQKD
SFVLNYPYFNNSYVKGANDNPLLGGFTLTGFEGWGGSNDSFNTYMTGTFAENLPTKFLQH
YLVYKWENYEDGQSPAGNHEKQITLKNEAGDTVVVTRKEEQREDNYIERIITLNGKKVLE
DVTYLLPWTDSDTNKEKLYHYNYEGGTTTWELQDDWADLKNVIVYKLTDTGRTEEQTVAV
VDGTVTLTAEANTPYVVVKGEEAPKTVTSWSNGAHVKDTGFNSYAGTGAGDALDENVWSG
DVKNDNVKVVRVATGNKYLSMGSEEQGLEVTTAIKDLTPGKNYVAEVYVDNKSDAKAWIE
VSAGTETVSNYTLKSFAGNYVQCDAHNNRGMANSNMQVMLVSFTAEKAIASLTLKREAGT
GITYFDDIRIVEKTLNNIQEDGSFKQDFEEVVQGIYPFVIGSAQGVTDQVTHLAEKHDPY
TQSGWGNVILDDVIGGTWSLKHHGNNSGIIYQTIPQNLHLEPGVTYKVSFDYQAGRDNMY
RIVTGDGDQITQTFDYLAGTAASAVGGKSTTGKFEFTITGSGTGQSWFGLSSTSFTEQKG
VDYCYGQKDFILDNLVVTPQEGLTLNETDVELKGVGDSRKLTASQEVTWSTSDENVVTVG
TDGYIQAAGAGTATITATAKTNTALTASCTVTVATSSEVVNESPFEAVAANTEQGGDEGK
EKAIDGNTSTHWHSNWSTGFTVSETNPAILTATLKDDAKDFGGFYITQRLGNVNGLISKL
TYVAGNTFDAATNTVSDVVKEGTVEVPSASVSAGATFGVAFGENINAKYLQIRVLNGGGN
FAAIAELRTCLKVAFTDEELLEEKQNAKTASDAAATTADEALTNAQTALTAAKAAEAAAE
DKIAANVAVKEAELAVKQAELNKQEAVKRQQEAAVALALMEASQSEDATVKQQKLAAAET
AKTAIATAETEILSINAALTMARYDAITAKSALEVQTKAAAVNDAKTVYDSTLQPLTDAN
SKLEQAKAAKEEADKTGTELEKAEANLRLAEAELAVAEAVQNQALAQCAWETAKAEAASA
RANVAATDEEATSAREEEKTANEAAAAARVQIANGKKTISACNSSLAKAEKVIQDLKSST
TELAEALDKAEAVYKAGGAAYTEASWKKFVTAYENARAAVGDSSKTPDELYELQKTLEDA
QKALEKKPDVQDSLAEAKKTLSETLKAAEAVYAAGGAAYTEASWKAFAAAYAAAKNPAAN
ADAAALKALAEALKKAQSGLVKAATPAPAPTEKLKKGDTVTVKNVVYKVTNADKKTVAAV
KGKNKKLASVSIASTVKVKGETCKVTAVNAKAFKGYARLKKVTIGKNVTTVGKQAFYGCK
KLTKVSFKGTAVKSIKSAAFKKANAKIKVTLPKKLKGKNRTKLVKQLKKAGIKSAK

Enzyme Prediction     

EC	3.2.1.97

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH101	324	1039	2.2e-236	0.9943422913719944

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
pfam12905	Glyco_hydro_101	2.86e-125	559	884	1	272	Endo-alpha-N-acetylgalactosaminidase. Virulence of pathogenic organisms such as the Gram-positive Streptococcus pneumoniae is largely determined by the ability to degrade host glycoproteins and to metabolize the resultant carbohydrates. This family is the enzymatic region, EC:3.2.1.97, of the cell surface proteins that specifically cleave Gal-beta-1,3-GalNAc-alpha-Ser/Thr (T-antigen, galacto-N-biose), the core 1 type O-linked glycan common to mucin glycoproteins. This reaction is exemplified by the S. pneumoniae protein Endo-alpha-N-acetylgalactosaminidase, where Asp764 is the catalytic nucleophile-base and Glu796 the catalytic proton donor.
cd14244	GH_101_like	8.99e-109	572	908	1	298	Endo-a-N-acetylgalactosaminidase and related glcyosyl hydrolases. This family contains the enzymatically active domain of cell surface proteins that specifically cleave Gal-beta-1,3-GalNAc-alpha-Ser/Thr (T-antigen, galacto-N-biose), the core 1 type O-linked glycan common to mucin glycoproteins (EC:3.2.1.97). It has been classified as glycosyl hydrolase family 101 in the Cazy resource. Virulence of pathogenic organisms such as the Gram-positive Streptococcus pneumoniae and other commensal human bacteria is largely determined by their ability to degrade host glycoproteins and to metabolize the resultant carbohydrates.
pfam18080	Gal_mutarotas_3	2.90e-67	320	558	1	243	Galactose mutarotase-like fold domain. This domain is found in endo-alpha-N-acetylgalactosaminidase present in Streptococcus pneumoniae. Endo-alpha-N-acetylgalactosaminidase is a cell surface-anchored glycoside hydrolase involved in the breakdown of mucin type O-linked glycans. The domain, known as domain 2, exhibits strong structural similarlity to the galactose mutarotase-like fold but lacks the active site residues. Domains, found in a number of glycoside hydrolases, structurally similar to domain 2 confer stability to the multidomain architectures.
pfam17974	GalBD_like	6.31e-64	1206	1374	1	173	Galactose-binding domain-like. Proteins containing a galactose-binding domain-like fold can be found in several different protein families, in both eukaryotes and prokaryotes. The common function of these domains is to bind to specific ligands, such as cell-surface-attached carbohydrate substrates for galactose oxidase and sialidase, phospholipids on the outer side of the mammalian cell membrane for coagulation factor Va, membrane-anchored ephrin for the Eph family of receptor tyrosine kinases, and a complex of broken single-stranded DNA and DNA polymerase beta for XRCC1. The structure of the galactose-binding domain-like members consists of a beta-sandwich, in which the strands making up the sheets exhibit a jellyroll fold.
pfam17451	Glyco_hyd_101C	6.86e-32	891	1013	1	111	Glycosyl hydrolase 101 beta sandwich domain. Virulence of pathogenic organisms such as the Gram-positive Streptococcus pneumoniae is largely determined by the ability to degrade host glycoproteins and to metabolize the resultant carbohydrates. This family is the enzymatic region, EC:3.2.1.97, of the cell surface proteins that specifically cleave Gal-beta-1,3-GalNAc-alpha-Ser/Thr (T-antigen, galacto-N-biose), the core 1 type O-linked glycan common to mucin glycoproteins. This reaction is exemplified by a S. pneumoniae protein, where Asp764 is the catalytic nucleophile-base and Glu796 the catalytic proton donor. This domain represents C-terminal the beta sandwich domain.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QNM14139.1	0.0	42	1643	248	1820
QWH41973.1	2.65e-274	40	1395	58	1445
APC49531.1	1.90e-273	27	1395	24	1427
BAL17718.1	3.86e-272	40	1395	32	1419
AYY26584.1	8.14e-272	40	1395	58	1445

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
2ZXQ_A	4.42e-239	314	1628	21	1370	Crystalstructure of endo-alpha-N-acetylgalactosaminidase from Bifidobacterium longum (EngBF) [Bifidobacterium longum]
6QEP_A	1.34e-234	313	1437	5	1157	EngBFDARPin Fusion 4b H14 [Bifidobacterium longum]
6QEV_B	2.82e-234	320	1437	12	1157	EngBFDARPin Fusion 4b B6 [Bifidobacterium longum]
6SH9_B	2.82e-234	320	1437	12	1157	EngBFDARPin Fusion 4b D12 [Bifidobacterium longum subsp. longum JCM 1217]
6QFK_A	2.82e-234	320	1437	12	1157	EngBFDARPin Fusion 4b G10 [Bifidobacterium longum]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
Q2MGH6	4.26e-206	58	1627	153	1622	Endo-alpha-N-acetylgalactosaminidase OS=Streptococcus pneumoniae serotype 4 (strain ATCC BAA-334 / TIGR4) OX=170187 GN=SP_0368 PE=1 SV=1
Q8DR60	1.12e-205	58	1556	153	1559	Endo-alpha-N-acetylgalactosaminidase OS=Streptococcus pneumoniae (strain ATCC BAA-255 / R6) OX=171101 GN=spr0328 PE=1 SV=1
A9WNA0	1.32e-129	320	1397	52	1034	Putative endo-alpha-N-acetylgalactosaminidase OS=Renibacterium salmoninarum (strain ATCC 33209 / DSM 20767 / JCM 11484 / NBRC 15589 / NCIMB 2235) OX=288705 GN=RSal33209_1326 PE=3 SV=2

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.000535	0.998537	0.000205	0.000288	0.000223	0.000185

TMHMM  Annotations     

start	end
12	34