CAZyme Information: MGYG000000924_01862

Basic Information

• Species: An172 sp002160515
• Lineage: Bacteria; Firmicutes_A; Clostridia; Oscillospirales; Acutalibacteraceae; An172; An172 sp002160515
• CAZyme ID: MGYG000000924_01862
• CAZy Family: GH101
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
2077		229969.56	4.2517

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000000924	2081481	MAG	China	Asia

• Gene Location: Start: 231; End: 6464 Strand: +

Full Sequence      

MRSTIKIRKITALLLAVIMLLGQSAAVSAADNDPANENYGWKVAQFGKIGDITVPDGGME
YYEDGFKETHTPFYKAGTSSIVIYNENAPELTNSVWEFDLIFQDDVQKCRIGFFPRFQDS
QNCGGIAIDKVASLQASTMQGGSESWPALANDTGFSIEHNREYHFKFLTDETSIKMYVDG
KLITQRTQMNNLPASGKYAFRIWGIADAEKGQKYVQVSNMRFAEYKKSQANKTDFEIQSS
DWGLSDVEIPLTLAQDDTVTAVKNGVEALAADTDYTLSENKLVISKDYIAKQADTFSLNV
EFNGGSQSQVTFTKKVPFEQFEYVMDFTTQQEGVVQKGGSGTIEYTEDGLHIKGGTTLHF
ENAPLAENGEIELVFEELNDDGGVGFLYRIDGDVWQSVYNANSSRYQYDTSLWNYRSNKT
SSYQILEDGTPIFKRNGYNHTLKLRYVGNTVTMWLDGQHMKDIRIASMDSLKGIIGLSTE
DATEIILKKVTYKNIDNLQLSESSEEKTISLGDMSVKLAADFPRVISYELNSKTMYGSQL
AKNYVSINGWDYPATAEISEETETSVTYNVKLEELGVSFDVVYTVLENNVLDMRIKNIAD
DSFTVYTIGFPEQPLISANSSQSGAKLDASMGASDVHLDLTKNSADKVLNTVCTIPVITT
DEMSASMANNVLNNLSEFTYRSFDMEDGTTTTGFWNTEFLYKGLDGARMYEDEDLYCQVV
LTEDTNEDGIMDWQDGANALKTIIDDKIVGGETVRNSTIHVGYNFSSVVQHPFLKVADNM
KRLSNYMDGFGQILVFKGYANEGHDSGHADYADINQRAGGAEDMQLLTEIIEDIDGVMGI
YINHSEAYPEAKMFTDETMTTRNGWKWLDQSKYIRREVDILNGGMDSRLDEMFEKVPGIG
FVYVDTYRDDRYTAAKLAENLSDEHGVIMGTEDGVKFDRWTAWVHWPSIGDGMHRFVYHQ
DKDVYKGSSLFRGGYNRSGGSGFMSWQHTNNINDTVKMFYTSQLPQKYLMHYDVRKMTSN
EAIFEGNVRSVNNGGVNEIYKDGNLIAKGDLVFIPWYAEDSETKNPDEAAKIYHWNPSSS
TTEWILPAGWENQTTVKFYKTTQNGKVFVEEIEVKDGKVSINAQANTPYVIYKGNEDVAP
DVTKWSEGSPIEDTSFNSRDFSIWQASSESGETDHITIEDDGNGVAIVNIRGAEDGRLSQ
TMTGLVLGQKYRVSVWTGATNGKTSRLTVVNSDGTEYENYTENTVRQSYLLDHYSQGKMM
QRIWVDFVATGETAEIILSADKSGDNGLVQFMETRIVKTDAPELPEGYAAYEDFEHVDQA
HGIFVPESREEHSHLSQTHEGYTTDTISGEWSLKMGTSFRTLPSTVRLLPNTKYTMEFKT
IGSGNVSVFSETTNVKVLDYNFEEGENKVEFTTDGSKDTVIRFAGNGVMDGAKLILDDFM
VYSIVDNTPPTVPENLKGESVNGNAVNLTWDASTDEDTGISGYNVYRNGELIGSTTETSY
TDNNVSEFTVYSYSVSAINLGSTESDKSAEIKVAVGNDNSLPVLTGIKPISNDKFVVTFS
KAMDKESAEKTENYIVSDGLKVTSAVLGDDLKSVTLTIEGVTSDTNAVLYTENIADNTVS
KNICSSASTVTVSLLSRWYKMDETDGDTAYDFTGNENGVKAGKYNYTEGKNGYAMTFNGG
EKIQLGDTLFNESDNWTISTWFNVTGETGQSQTILSNNTSGDASSKGMWFYINGGSKKLC
VSLSDSNGNGSIYLETPQAVSLNTWNHTALVKDGDTLRLYLNGVLAGEKSIAGLSTKNCP
NDVRIGGNFNGSGSFLHGFKGAMDDFRVFASALDESQVVNEYEKVYDVPTISGDKFYYDK
SLGGDLQIDVNYNGYTAESVICGEEELLNSAVTVTPNGFVFAENWLNNIDGECTVEVTFI
KGEDSQVISFNIEAIASALKPDKSELFFLKAEAEELDNVYTVNSYKALQEKIALAEQIIT
NPDATESDITDAVNQLTAAMESLKYNKGDVNGDGKIDIIDTSLIQTYIIRKEVSGDFYLD
PADSDGNGTINILDATMTQLVASGNMIINDDGDIVKA

Enzyme Prediction     

No EC number prediction in MGYG000000924_01862.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH101	513	1027	1.2e-85	0.801980198019802

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
cd14244	GH_101_like	4.09e-63	750	1019	4	298	Endo-a-N-acetylgalactosaminidase and related glcyosyl hydrolases. This family contains the enzymatically active domain of cell surface proteins that specifically cleave Gal-beta-1,3-GalNAc-alpha-Ser/Thr (T-antigen, galacto-N-biose), the core 1 type O-linked glycan common to mucin glycoproteins (EC:3.2.1.97). It has been classified as glycosyl hydrolase family 101 in the Cazy resource. Virulence of pathogenic organisms such as the Gram-positive Streptococcus pneumoniae and other commensal human bacteria is largely determined by their ability to degrade host glycoproteins and to metabolize the resultant carbohydrates.
pfam12905	Glyco_hydro_101	2.40e-60	734	993	1	270	Endo-alpha-N-acetylgalactosaminidase. Virulence of pathogenic organisms such as the Gram-positive Streptococcus pneumoniae is largely determined by the ability to degrade host glycoproteins and to metabolize the resultant carbohydrates. This family is the enzymatic region, EC:3.2.1.97, of the cell surface proteins that specifically cleave Gal-beta-1,3-GalNAc-alpha-Ser/Thr (T-antigen, galacto-N-biose), the core 1 type O-linked glycan common to mucin glycoproteins. This reaction is exemplified by the S. pneumoniae protein Endo-alpha-N-acetylgalactosaminidase, where Asp764 is the catalytic nucleophile-base and Glu796 the catalytic proton donor.
pfam18080	Gal_mutarotas_3	1.89e-41	509	733	1	243	Galactose mutarotase-like fold domain. This domain is found in endo-alpha-N-acetylgalactosaminidase present in Streptococcus pneumoniae. Endo-alpha-N-acetylgalactosaminidase is a cell surface-anchored glycoside hydrolase involved in the breakdown of mucin type O-linked glycans. The domain, known as domain 2, exhibits strong structural similarlity to the galactose mutarotase-like fold but lacks the active site residues. Domains, found in a number of glycoside hydrolases, structurally similar to domain 2 confer stability to the multidomain architectures.
pfam17451	Glyco_hyd_101C	1.22e-24	1004	1107	3	111	Glycosyl hydrolase 101 beta sandwich domain. Virulence of pathogenic organisms such as the Gram-positive Streptococcus pneumoniae is largely determined by the ability to degrade host glycoproteins and to metabolize the resultant carbohydrates. This family is the enzymatic region, EC:3.2.1.97, of the cell surface proteins that specifically cleave Gal-beta-1,3-GalNAc-alpha-Ser/Thr (T-antigen, galacto-N-biose), the core 1 type O-linked glycan common to mucin glycoproteins. This reaction is exemplified by a S. pneumoniae protein, where Asp764 is the catalytic nucleophile-base and Glu796 the catalytic proton donor. This domain represents C-terminal the beta sandwich domain.
pfam13385	Laminin_G_3	3.04e-22	1677	1838	1	150	Concanavalin A-like lectin/glucanases superfamily. This domain belongs to the Concanavalin A-like lectin/glucanases superfamily.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
ATD58436.1	4.99e-203	36	1452	235	1634
ATD54117.1	4.99e-203	36	1452	235	1634
QBJ76357.1	4.99e-203	36	1452	235	1634
SLK22612.1	4.99e-203	36	1452	235	1634
AZN39795.1	2.30e-179	319	1392	18	1093

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
6QEP_A	4.53e-81	507	1378	10	1026	EngBFDARPin Fusion 4b H14 [Bifidobacterium longum]
6SH9_B	4.62e-81	507	1378	10	1026	EngBFDARPin Fusion 4b D12 [Bifidobacterium longum subsp. longum JCM 1217]
6QFK_A	4.62e-81	507	1378	10	1026	EngBFDARPin Fusion 4b G10 [Bifidobacterium longum]
6QEV_B	4.62e-81	507	1378	10	1026	EngBFDARPin Fusion 4b B6 [Bifidobacterium longum]
2ZXQ_A	5.23e-81	507	1378	25	1041	Crystalstructure of endo-alpha-N-acetylgalactosaminidase from Bifidobacterium longum (EngBF) [Bifidobacterium longum]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
A9WNA0	4.16e-76	508	1378	51	961	Putative endo-alpha-N-acetylgalactosaminidase OS=Renibacterium salmoninarum (strain ATCC 33209 / DSM 20767 / JCM 11484 / NBRC 15589 / NCIMB 2235) OX=288705 GN=RSal33209_1326 PE=3 SV=2
Q2MGH6	6.20e-74	368	1389	177	1330	Endo-alpha-N-acetylgalactosaminidase OS=Streptococcus pneumoniae serotype 4 (strain ATCC BAA-334 / TIGR4) OX=170187 GN=SP_0368 PE=1 SV=1
Q8DR60	5.51e-73	368	1389	177	1330	Endo-alpha-N-acetylgalactosaminidase OS=Streptococcus pneumoniae (strain ATCC BAA-255 / R6) OX=171101 GN=spr0328 PE=1 SV=1
P20533	1.28e-10	1445	1632	460	642	Chitinase A1 OS=Niallia circulans OX=1397 GN=chiA1 PE=1 SV=1
A0P8X0	1.71e-10	1427	1543	794	907	Alpha-amylase OS=Niallia circulans OX=1397 GN=igtZ PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.000238	0.999071	0.000193	0.000162	0.000154	0.000145

TMHMM  Annotations     

There is no transmembrane helices in MGYG000000924_01862.