CAZyme Information: MGYG000000924_00733

Basic Information

• Species: An172 sp002160515
• Lineage: Bacteria; Firmicutes_A; Clostridia; Oscillospirales; Acutalibacteraceae; An172; An172 sp002160515
• CAZyme ID: MGYG000000924_00733
• CAZy Family: CBM32
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
1420		155755.77	3.961

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000000924	2081481	MAG	China	Asia

• Gene Location: Start: 83538; End: 87800 Strand: +

Full Sequence      

MQKKFTRKVLSVLLALTMITGMFSMISFSSSAEVVKTNVALNATATADGQDSDAHAASKA
VDGVINREATANADQSRWAVEDEGGQGTPASHWLKVDLGSSKTFNEVVIDWERKNVTGYK
VEVSDNDADYKEIYSSTSLNTFSDSIVLDEAVTAQYVKVTINSYTREGNTLSGDGTEVAG
PAWSSVSIYEIQLLQYAQNDNLATGKTATANGSENNDFTAPKAVDGDDSTRWASKQANAP
HWIQVDLGQSTEVAAATIHWERNTVKSFEIQYSDTAEEGSWKTAYSYSSTELYNGWNTKV
NFDTPATGRYFRIYIKDFADKGQDESGKEVTWNTISIFEIGLFAEPLVISDHTLAEVVNS
ITAPEITADDTTVPLPEVPEGFKVTFAGSNYDNVITPEGNIATPLVDKQVKVAYEVTDGQ
DTKRTADLTVTVPGKVAASAEENNAKPVVIPEIAEWIGSTGDMTITESSKVVYASEDLKA
TADLFAADYLEVTGMELAVTAGTKEDVAAGDFYLALDAEDTMNLRDEGYYVNIADGAAYI
TANTATGARWATVTFLQILKQNGTTIPQGEIRDYPKYEIRGFMLDVGRIPIEMETIYDIA
KTMSWYKLNDFELHLNDNLIFLEDYVNAGLDPLDAYSAFRMESDITNDEGEGITADDLYY
TKDEFRKLITDSRAIGVNIVPEFDFPAHSLAITSVFDELAVDYINESGRSYADHFDLSNP
ESTELIKDIWDEYLDGENPVFDELTTVNVGADEYTHNNEDYRKFTDTILKYIQQEKGRTV
RIWGSLSSITGTIPVHSEDVQMYIWNTGWANPKDMLEQGYDLININDGSVYIVPGANYYY
DYLNPTSIYNNWKPNVIGGTTIAETEDQMLGGAYAIWNDCIDTRDNGIYEYDNFDRFYKP
VAAFSEKLWGEGTEKTATELAETFEETSTAPGTNPYNEVETTANDKTFAEYTFDDGTGTD
VTGNGYNLTDNSENVTYTDIAEGNQALVLNGDTSYVETPIEYLPVDTDLTIRVKRTSYSN
EEQILFESDYQPTNGAGKYAIQAVQADTGKVGFVRGARTYTFDYELPVGEWVTLTFRGEQ
NFASLYVNGELVDSLGSTAGLAMRATLSSPFNRIGSKTNAFIGLVDYVKADNLNAPDETI
ISNDSFTVECDNQNPAAGSEGPISYAFDNNLNTFWHSNYTPMQALPANIEIHANEGTTLD
VGAITYVPRQSGDNGYITSATFYYKNTEAGVNEWTEITSVNWAADSSTKKVNFDSIISAT
DIKITVKAGKNNYGSAAEFYLHNQMDASNAEALNNAKANADEVIAEYADIMNNEKFAEAY
NKAAELLENGGTQEEYDAAASALAAMNAVVIRGGDIDGSGNVTVSDATIIQLIVSGKTID
NADTYSKYSDIDLDGSVDIEDATIIQTYLTGASDIFGKTV

Enzyme Prediction     

No EC number prediction in MGYG000000924_00733.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH20	573	910	2.7e-58	0.9673590504451038
CBM32	205	340	1.3e-22	0.9516129032258065
CBM32	44	191	6.4e-21	0.9354838709677419

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
cd06564	GH20_DspB_LnbB-like	3.12e-118	578	910	1	325	Glycosyl hydrolase family 20 (GH20) catalytic domain of dispersin B (DspB), lacto-N-biosidase (LnbB) and related proteins. Dispersin B is a soluble beta-N-acetylglucosamidase found in bacteria that hydrolyzes the beta-1,6-linkages of PGA (poly-beta-(1,6)-N-acetylglucosamine), a major component of the extracellular polysaccharide matrix. Lacto-N-biosidase hydrolyzes lacto-N-biose (LNB) type I oligosaccharides at the nonreducing terminus to produce lacto-N-biose as part of the GNB/LNB (galacto-N-biose/lacto-N-biose I) degradation pathway. The lacto-N-biosidase from Bifidobacterium bifidum has this GH20 domain, a carbohydrate binding module 32, and a bacterial immunoglobulin-like domain 2, as well as a YSIRK signal peptide and a G5 membrane anchor at the N and C termini, respectively. The GH20 hexosaminidases are thought to act via a catalytic mechanism in which the catalytic nucleophile is not provided by solvent or the enzyme, but by the substrate itself.
pfam00728	Glyco_hydro_20	3.47e-28	577	909	1	343	Glycosyl hydrolase family 20, catalytic domain. This domain has a TIM barrel fold.
cd02742	GH20_hexosaminidase	1.74e-27	579	910	1	303	Beta-N-acetylhexosaminidases of glycosyl hydrolase family 20 (GH20) catalyze the removal of beta-1,4-linked N-acetyl-D-hexosamine residues from the non-reducing ends of N-acetyl-beta-D-hexosaminides including N-acetylglucosides and N-acetylgalactosides. These enzymes are broadly distributed in microorganisms, plants and animals, and play roles in various key physiological and pathological processes. These processes include cell structural integrity, energy storage, cellular signaling, fertilization, pathogen defense, viral penetration, the development of carcinomas, inflammatory events and lysosomal storage disorders. The GH20 enzymes include the eukaryotic beta-N-acetylhexosaminidases A and B, the bacterial chitobiases, dispersin B, and lacto-N-biosidase. The GH20 hexosaminidases are thought to act via a catalytic mechanism in which the catalytic nucleophile is not provided by the solvent or the enzyme, but by the substrate itself.
COG3525	Chb	4.52e-23	429	909	109	613	N-acetyl-beta-hexosaminidase [Carbohydrate transport and metabolism].
pfam02838	Glyco_hydro_20b	2.76e-22	446	573	1	123	Glycosyl hydrolase family 20, domain 2. This domain has a zincin-like fold.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QQV05894.1	0.0	34	1356	40	1338
QPS14026.1	0.0	34	1356	40	1338
QMW75640.1	0.0	34	1356	40	1338
QQY27155.1	0.0	34	1356	40	1338
AYE35153.1	1.75e-291	198	1282	34	1116

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
6JQF_A	1.30e-112	356	935	2	573	Crystallizationanalysis of a beta-N-acetylhexosaminidase (Am2136) from Akkermansia muciniphila [Akkermansia muciniphila ATCC BAA-835]
4H04_A	9.29e-35	437	917	24	488	Lacto-N-biosidasefrom Bifidobacterium bifidum [Bifidobacterium bifidum JCM 1254],4H04_B Lacto-N-biosidase from Bifidobacterium bifidum [Bifidobacterium bifidum JCM 1254],4JAW_A Crystal Structure of Lacto-N-Biosidase from Bifidobacterium bifidum complexed with LNB-thiazoline [Bifidobacterium bifidum JCM 1254],4JAW_B Crystal Structure of Lacto-N-Biosidase from Bifidobacterium bifidum complexed with LNB-thiazoline [Bifidobacterium bifidum JCM 1254],5BXP_A LNBase in complex with LNB-LOGNAc [Bifidobacterium bifidum JCM 1254],5BXP_B LNBase in complex with LNB-LOGNAc [Bifidobacterium bifidum JCM 1254],5BXR_A LNBase in complex with LNB-NHAcDNJ [Bifidobacterium bifidum JCM 1254],5BXR_B LNBase in complex with LNB-NHAcDNJ [Bifidobacterium bifidum JCM 1254],5BXS_A LNBase in complex with LNB-NHAcCAS [Bifidobacterium bifidum JCM 1254],5BXS_B LNBase in complex with LNB-NHAcCAS [Bifidobacterium bifidum JCM 1254],5BXT_A LNBase in complex with LNB-NHAcAUS [Bifidobacterium bifidum JCM 1254],5BXT_B LNBase in complex with LNB-NHAcAUS [Bifidobacterium bifidum JCM 1254]
3S6T_A	3.30e-19	495	911	93	532	CrystalStructure of insect beta-N-acetyl-D-hexosaminidase OfHex1 V327G complexed with PUGNAc [Ostrinia furnacalis]
3NSM_A	5.69e-19	495	911	90	529	CrystalStructure of insect beta-N-acetyl-D-hexosaminidase OfHex1 from Ostrinia furnacalis [Ostrinia furnacalis],3NSN_A Crystal Structure of insect beta-N-acetyl-D-hexosaminidase OfHex1 complexed with TMG-chitotriomycin [Ostrinia furnacalis],3OZO_A Crystal Structure of insect beta-N-acetyl-D-hexosaminidase OfHex1 complexed with NGT [Ostrinia furnacalis],3OZP_A Crystal Structure of insect beta-N-acetyl-D-hexosaminidase OfHex1 complexed with PUGNAc [Ostrinia furnacalis]
3VTR_A	3.04e-18	495	911	90	529	CrystalStructure of insect beta-N-acetyl-D-hexosaminidase OfHex1 E328A complexed with TMG-chitotriomycin [Ostrinia furnacalis]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
B2UPR7	1.09e-115	356	935	24	595	Beta-hexosaminidase Amuc_2136 OS=Akkermansia muciniphila (strain ATCC BAA-835 / DSM 22959 / JCM 33894 / BCRC 81048 / CCUG 64013 / CIP 107961 / Muc) OX=349741 GN=Amuc_2136 PE=1 SV=1
Q8WSF3	2.36e-18	455	914	152	615	Probable beta-hexosaminidase fdl OS=Drosophila melanogaster OX=7227 GN=fdl PE=1 SV=1
Q06GJ0	3.38e-18	495	911	112	551	Chitooligosaccharidolytic beta-N-acetylglucosaminidase OS=Ostrinia furnacalis OX=93504 PE=1 SV=1
Q9HGI3	1.29e-16	526	909	128	545	Beta-hexosaminidase OS=Emericella nidulans OX=162425 GN=nagA PE=1 SV=1
P49008	1.58e-15	445	700	23	283	Beta-hexosaminidase OS=Porphyromonas gingivalis (strain ATCC BAA-308 / W83) OX=242619 GN=nahA PE=3 SV=2

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.000778	0.997884	0.000745	0.000216	0.000183	0.000161

TMHMM  Annotations     

start	end
9	31