CAZyme Information: MGYG000000874_00648

Basic Information

• Species: CAG-267 sp900551865
• Lineage: Bacteria; Proteobacteria; Alphaproteobacteria; RF32; CAG-239; CAG-267; CAG-267 sp900551865
• CAZyme ID: MGYG000000874_00648
• CAZy Family: GH77
• CAZyme Description: 4-alpha-glucanotransferase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
526		60615.63	7.6981

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000000874	1307174	MAG	United States	North America

• Gene Location: Start: 92635; End: 94215 Strand: +

Full Sequence      

MKHAEKYINGDSYNRSAGILLPVFSLPSPYGIGDMGEGAYRFVDMLREAGQTLWEVLPHG
QTGYCNSPYKTLSAFAGNPYFIAPDMLEKDGLLLPGEAEQYDFGANSADIDYGRLFAARF
KLLRLAFDRFCARGGKSDSGFLAFRRKNAVWLSDYALFMALKEHFGWKPWNEWEEGAAFR
QPEVLRGYRRRLAVSVLFWQFVQYEFFCQWHQLKEYAGRNGIKIIGDLPFYVAYDSADVW
SRRNIFAVNPEDASVTLSAGVPGDCFASHTRSWDMPCYDWPAAAADKFRWHLARFRFYAG
LYDVIRIDHAIGFVRYYGLGLGSERWYDGPDAENDVFIPKLTRLAKACGVELIAEDLGSV
PERAYRLFTKYRWAGTRVLQFGFANGYGTETIHLPFYYPHKSVAYTGTHDNPAFASYLQG
LETKYQPYLRYYLQASSQDDLQKRCIEELYRSAAEKVIIPLPDILGLDNRGRIAHGRDYA
KSWRWRLSSLDDVSEEIQQWLKKLAFAYARIPFSHEQGKEYGWNWK

Enzyme Prediction     

No EC number prediction in MGYG000000874_00648.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH77	21	505	1.4e-123	0.9878542510121457

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
PRK14508	PRK14508	1.13e-178	15	511	5	497	4-alpha-glucanotransferase; Provisional
pfam02446	Glyco_hydro_77	4.40e-148	21	488	1	458	4-alpha-glucanotransferase. These enzymes EC:2.4.1.25 transfer a segment of a (1,4)-alpha-D-glucan to a new 4-position in an acceptor, which may be glucose or (1,4)-alpha-D-glucan.
COG1640	MalQ	1.63e-113	6	512	5	516	4-alpha-glucanotransferase [Carbohydrate transport and metabolism].
PLN02635	PLN02635	2.17e-100	2	520	15	535	disproportionating enzyme
PRK14510	PRK14510	1.75e-90	7	510	715	1220	bifunctional glycogen debranching protein GlgX/4-alpha-glucanotransferase.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
CCG34906.1	4.27e-122	15	510	3	496
AYF39757.1	1.26e-117	15	511	11	500
ADU28083.1	1.26e-117	15	511	11	500
AYF42590.1	1.26e-117	15	511	11	500
QCN93338.1	1.26e-117	15	511	11	500

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
6M6T_A	4.28e-89	15	510	5	496	ChainA, 4-alpha-glucanotransferase [Streptococcus agalactiae],6M6T_B Chain B, 4-alpha-glucanotransferase [Streptococcus agalactiae],6M6T_C Chain C, 4-alpha-glucanotransferase [Streptococcus agalactiae],6M6T_D Chain D, 4-alpha-glucanotransferase [Streptococcus agalactiae],6M6T_E Chain E, 4-alpha-glucanotransferase [Streptococcus agalactiae],6M6T_F Chain F, 4-alpha-glucanotransferase [Streptococcus agalactiae],6M6T_G Chain G, 4-alpha-glucanotransferase [Streptococcus agalactiae],6M6T_H Chain H, 4-alpha-glucanotransferase [Streptococcus agalactiae]
1TZ7_A	1.34e-86	12	510	19	503	Aquifexaeolicus amylomaltase [Aquifex aeolicus],1TZ7_B Aquifex aeolicus amylomaltase [Aquifex aeolicus]
2X1I_A	1.49e-83	18	511	8	500	glycosidehydrolase family 77 4-alpha-glucanotransferase from thermus brockianus [Thermus brockianus]
1FP8_A	2.65e-80	15	511	5	500	StructureOf The Amylomaltase From Thermus Thermophilus Hb8 In Space Group P21212 [Thermus thermophilus],1FP9_A Structure Of Amylomaltase From Thermus Thermophilus Hb8 In Space Group C2 [Thermus thermophilus]
1CWY_A	5.22e-80	15	511	5	500	CrystalStructure Of Amylomaltase From Thermus Aquaticus, A Glycosyltransferase Catalysing The Production Of Large Cyclic Glucans [Thermus aquaticus],1ESW_A X-Ray Structure Of Acarbose Bound To Amylomaltase From Thermus Aquaticus. Implications For The Synthesis Of Large Cyclic Glucans [Thermus aquaticus]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
Q59266	6.14e-104	23	510	3	485	4-alpha-glucanotransferase OS=Clostridium butyricum OX=1492 GN=malQ PE=1 SV=1
P72785	1.50e-96	15	501	5	486	4-alpha-glucanotransferase OS=Synechocystis sp. (strain PCC 6803 / Kazusa) OX=1111708 GN=malQ PE=3 SV=1
P0A3Q1	4.60e-95	15	511	4	496	4-alpha-glucanotransferase OS=Streptococcus pneumoniae (strain ATCC BAA-255 / R6) OX=171101 GN=malQ PE=3 SV=1
P0A3Q0	4.60e-95	15	511	4	496	4-alpha-glucanotransferase OS=Streptococcus pneumoniae serotype 4 (strain ATCC BAA-334 / TIGR4) OX=170187 GN=malQ PE=3 SV=1
O66937	8.40e-86	15	510	2	483	4-alpha-glucanotransferase OS=Aquifex aeolicus (strain VF5) OX=224324 GN=malQ PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000051	0.000000	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

There is no transmembrane helices in MGYG000000874_00648.