CAZyme Information: MGYG000000860_01734

Basic Information

• Lineage: Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales; Pasteurellaceae; Haemophilus_D
• CAZyme ID: MGYG000000860_01734
• CAZy Family: GT51
• CAZyme Description: Penicillin-binding protein 1A

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
501		54238.35	5.2721

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000000860	1828071	MAG	China	Asia

• Gene Location: Start: 95; End: 1600 Strand: +

Full Sequence      

MTLSSNAMRWAGKSPVKVGEQIWIRKRDNGEWVLGQIPAANSALVSLNSDNGAIEAMVGG
FSYEQSKFNRATQSLVQVGSSIKPFIYAAALEKGLTLSSVLQDSPISIQKPGQPLWQPKN
SPDRYDGPMRLRVGLGQSKNMIAIRALQIAGVGFTADFLQRFGFKRDQYFASEALALGAA
SFTPLEMARAYAVFDNGGFLIDPYLIEKIQDNTGKDLFIANPKIACITCNDIPVIYGETK
DKIDGFKDVAEVANPDNLKSAKGNSNTDTDEGEGDQQPENVPDLPELQTSALNDGSVDLM
ADAKDGAAKQEYAPRVISGELAFLIRSALNTAIYGEQGLSWKGTSWRIAQSIKRSDIGGK
TGTTNSAKVAWYAGFGANLVTTTYVGFDDNKRVLGKGEAGAKTAMPAWVAYMKAALSDIP
ERQLALPPNIMEKTIDSNSGLLSEGGGRKEYFIVGTEPKRTYIAEMKERGYYVPPELQQR
LNGGTGKTKDAIPATQPEELF

Enzyme Prediction     

EC	2.4.1.129

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
COG5009	MrcA	0.0	1	490	371	795	Membrane carboxypeptidase/penicillin-binding protein [Cell wall/membrane/envelope biogenesis].
PRK11636	mrcA	6.96e-180	1	459	372	829	penicillin-binding protein 1a; Provisional
TIGR02074	PBP_1a_fam	1.27e-73	40	418	255	531	penicillin-binding protein, 1A family. Bacterial that synthesize a cell wall of peptidoglycan (murein) generally have several transglycosylases and transpeptidases for the task. This family consists of bifunctional transglycosylase/transpeptidase penicillin-binding proteins (PBP). In the Proteobacteria, this family includes PBP 1A but not the paralogous PBP 1B (TIGR02071). This family also includes related proteins, often designated PBP 1A, from other bacterial lineages. [Cell envelope, Biosynthesis and degradation of murein sacculus and peptidoglycan]
COG0744	MrcB	1.03e-59	38	485	324	661	Membrane carboxypeptidase (penicillin-binding protein) [Cell wall/membrane/envelope biogenesis].
PRK09506	mrcB	9.46e-24	42	447	470	776	bifunctional glycosyl transferase/transpeptidase; Reviewed

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QOR25347.1	0.0	1	501	372	872
QOR23337.1	0.0	1	501	372	872
QOR14169.1	0.0	1	501	372	872
QOR19922.1	0.0	1	501	372	872
QAT94541.1	0.0	1	501	372	872

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
5U2G_A	2.37e-279	2	501	347	838	2.6Angstrom Resolution Crystal Structure of Penicillin-Binding Protein 1A from Haemophilus influenzae [Haemophilus influenzae Rd KW20],5U2G_B 2.6 Angstrom Resolution Crystal Structure of Penicillin-Binding Protein 1A from Haemophilus influenzae [Haemophilus influenzae Rd KW20]
4OON_A	2.03e-77	3	452	354	759	Crystalstructure of PBP1a in complex with compound 17 ((4Z,8S,11E,14S)-5-(2-amino-1,3-thiazol-4-yl)-14-(5,6-dihydroxy-1,3-dioxo-1,3-dihydro-2H-isoindol-2-yl)-8-formyl-2-methyl-6-oxo-3,10-dioxa-4,7,11-triazatetradeca-4,11-diene-2,12,14-tricarboxylic acid) [Pseudomonas aeruginosa PAO1]
3UDF_A	2.78e-71	16	420	361	720	ChainA, Penicillin-binding protein 1a [Acinetobacter baumannii],3UDF_B Chain B, Penicillin-binding protein 1a [Acinetobacter baumannii],3UDI_A Chain A, Penicillin-binding protein 1a [Acinetobacter baumannii],3UDI_B Chain B, Penicillin-binding protein 1a [Acinetobacter baumannii],3UDX_A Chain A, Penicillin-binding protein 1a [Acinetobacter baumannii],3UDX_B Chain B, Penicillin-binding protein 1a [Acinetobacter baumannii],3UE0_A Chain A, Penicillin-binding protein 1a [Acinetobacter baumannii],3UE0_B Chain B, Penicillin-binding protein 1a [Acinetobacter baumannii],3UE1_A Chain A, Penicillin-binding protein 1a [Acinetobacter baumannii],3UE1_B Chain B, Penicillin-binding protein 1a [Acinetobacter baumannii]
7U4H_A	1.56e-19	42	490	472	834	ChainA, Penicillin-binding protein 1A (Pbp1a) [Chlamydia trachomatis D/UW-3/CX],7U4H_B Chain B, Penicillin-binding protein 1A (Pbp1a) [Chlamydia trachomatis D/UW-3/CX]
5FGZ_A	2.39e-19	42	448	415	724	E.coli PBP1b in complex with FPI-1465 [Escherichia coli K-12],5HL9_A E. coli PBP1b in complex with acyl-ampicillin and moenomycin [Escherichia coli K-12],5HLA_A E. coli PBP1b in complex with acyl-cephalexin and moenomycin [Escherichia coli K-12],5HLB_A E. coli PBP1b in complex with acyl-aztreonam and moenomycin [Escherichia coli K-12],5HLD_A E. coli PBP1b in complex with acyl-CENTA and moenomycin [Escherichia coli K-12],6YN0_A Structure of E. coli PBP1b with a FtsN peptide activating transglycosylase activity [Escherichia coli K-12],7LQ6_A Chain A, Penicillin-binding protein 1B [Escherichia coli K-12]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
P31776	5.63e-286	1	501	361	853	Penicillin-binding protein 1A OS=Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd) OX=71421 GN=mrcA PE=1 SV=2
P02918	3.01e-137	1	466	372	836	Penicillin-binding protein 1A OS=Escherichia coli (strain K12) OX=83333 GN=mrcA PE=1 SV=1
Q9KNU5	1.67e-104	19	472	406	816	Penicillin-binding protein 1A OS=Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961) OX=243277 GN=mrcA PE=3 SV=2
Q87AW7	6.29e-84	1	436	377	775	Penicillin-binding protein 1A OS=Xylella fastidiosa (strain Temecula1 / ATCC 700964) OX=183190 GN=mrcA PE=3 SV=1
Q9PGD4	4.64e-83	1	436	377	775	Penicillin-binding protein 1A OS=Xylella fastidiosa (strain 9a5c) OX=160492 GN=mrcA PE=3 SV=2

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000053	0.000000	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

There is no transmembrane helices in MGYG000000860_01734.