CAZyme Information: MGYG000000628_00415

Basic Information

• Lineage: Bacteria; Firmicutes_A; Clostridia_A; Christensenellales; CAG-917; CAG-475
• CAZyme ID: MGYG000000628_00415
• CAZy Family: GH3
• CAZyme Description: Thermostable beta-glucosidase B

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
815	MGYG000000628_11|CGC2	90277.12	7.0694

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000000628	1636358	MAG	Madagascar	Africa

• Gene Location: Start: 24478; End: 26925 Strand: +

Full Sequence      

MNDKDYLEILKKLTLDEKVTMLSGSTNWLTQPIKRDDIDIPAVRMSDGPSGLRREKIGGG
VNIMQTPEPATCFPGAVTTASSWDEELVEEVGKAIAVEAQSLGVSTVLGPGVNIKRGPLC
GRNFEYFSEDPFLAGRMGGAWVHGVQEMNVGTSLKHYLANNQEYIRMTIDSIVDERTLRE
IYMPAFEHIVKEEQPTTVMCSYNRLNGTYLSDHKRFLTDILRDEWGFKGIVVSDWGAVND
RAEGVKAGLDLEMPGSKGCGLNGDTVYKALKAGTLTEEDIDKVIFRLIKFAYENSVREVE
GVTFDVEKHNAIARKAAESGAVLLKNDGILPLNKKQSIAVVGRLAKQIRYQGGGSSHILP
TKLTSFTDALDKVGQSYDYADGYVLKGEGYKKSLIKKAVKAAKDKDVVLLFVGLTDAFES
EGYDRHHIKMPSSHVTLINEILKVNKNVVIVLSCGSPVEIGDWDKDVKGILNLYLGGQAG
GEAAYNLIYGKVNPSGKLAETFPVHEDDYLGSKYFRMGPRTVEYREGVYVGYRYYDSANK
RVKYPFGYGLSYTQFEYSNLRLSANAINEDEPFTVTFTVKNVGKVAGAEIAQLYVNDVES
TLYRPTKELKGFKKVFLQPGEEKDVEINLDSRAFAYYNVAIKDWHVESGDFKILVGASSR
DIKLEGVVHVTSKNPDAQIPDYKAVAPCYYDIANATEIPVEQFEALYGAKMIENKPFAKG
ELQACNTIGQCRVSPFGKFMYNLCVGAVNLVALSSENPEMLTNSVKDMPYRTIAAWSMGI
ISKRSLDGLVDMLNGKKGGFRRFLKGFGKEKAKKK

Enzyme Prediction     

EC	3.2.1.21	3.2.1.-	3.2.1.45

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH3	37	254	4.3e-68	0.9722222222222222

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
PRK15098	PRK15098	1.24e-93	8	663	40	758	beta-glucosidase BglX.
COG1472	BglX	8.43e-67	40	356	60	372	Periplasmic beta-glucosidase and related glycosidases [Carbohydrate transport and metabolism].
PLN03080	PLN03080	1.22e-66	13	630	60	743	Probable beta-xylosidase; Provisional
pfam01915	Glyco_hydro_3_C	1.25e-50	321	532	1	216	Glycosyl hydrolase family 3 C-terminal domain. This domain is involved in catalysis and may be involved in binding beta-glucan. This domain is found associated with pfam00933.
pfam00933	Glyco_hydro_3	3.52e-39	22	288	48	314	Glycosyl hydrolase family 3 N terminal domain.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
AEV59720.1	1.33e-228	9	795	19	803
QEN03854.1	2.23e-224	8	807	7	800
CAJ35242.1	8.15e-223	4	796	2	795
CEP78497.1	9.14e-221	3	672	2	665
CEP78340.1	6.42e-220	3	672	2	665

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
7MS2_A	1.73e-208	5	794	4	752	ChainA, Thermostable beta-glucosidase B [Acetivibrio thermocellus AD2],7MS2_B Chain B, Thermostable beta-glucosidase B [Acetivibrio thermocellus AD2]
5WAB_A	2.16e-172	1	668	1	661	CrystalStructure of Bifidobacterium adolescentis GH3 beta-glucosidase [Bifidobacterium adolescentis ATCC 15703],5WAB_B Crystal Structure of Bifidobacterium adolescentis GH3 beta-glucosidase [Bifidobacterium adolescentis ATCC 15703],5WAB_C Crystal Structure of Bifidobacterium adolescentis GH3 beta-glucosidase [Bifidobacterium adolescentis ATCC 15703],5WAB_D Crystal Structure of Bifidobacterium adolescentis GH3 beta-glucosidase [Bifidobacterium adolescentis ATCC 15703]
2X40_A	4.12e-116	8	670	6	713	Structureof beta-glucosidase 3B from Thermotoga neapolitana in complex with glycerol [Thermotoga neapolitana DSM 4359],2X41_A Structure of beta-glucosidase 3B from Thermotoga neapolitana in complex with glucose [Thermotoga neapolitana DSM 4359]
2X42_A	6.14e-115	8	670	6	713	Structureof beta-glucosidase 3B from Thermotoga neapolitana in complex with alpha-D-glucose [Thermotoga neapolitana DSM 4359]
3AC0_A	1.42e-98	1	662	1	828	Crystalstructure of Beta-glucosidase from Kluyveromyces marxianus in complex with glucose [Kluyveromyces marxianus],3AC0_B Crystal structure of Beta-glucosidase from Kluyveromyces marxianus in complex with glucose [Kluyveromyces marxianus],3AC0_C Crystal structure of Beta-glucosidase from Kluyveromyces marxianus in complex with glucose [Kluyveromyces marxianus],3AC0_D Crystal structure of Beta-glucosidase from Kluyveromyces marxianus in complex with glucose [Kluyveromyces marxianus]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
P14002	1.68e-208	5	794	4	752	Thermostable beta-glucosidase B OS=Acetivibrio thermocellus (strain ATCC 27405 / DSM 1237 / JCM 9322 / NBRC 103400 / NCIMB 10682 / NRRL B-4536 / VPI 7372) OX=203119 GN=bglB PE=1 SV=2
F6C6C1	7.28e-175	1	662	1	664	Exo-alpha-(1->6)-L-arabinopyranosidase OS=Bifidobacterium breve (strain ACS-071-V-Sch8b) OX=866777 GN=HMPREF9228_1477 PE=1 SV=1
E7CY69	1.62e-173	1	662	1	664	Exo-alpha-(1->6)-L-arabinopyranosidase OS=Bifidobacterium longum OX=216816 GN=apy PE=1 SV=1
Q2U8Y5	2.12e-106	5	662	5	823	Probable beta-glucosidase I OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=bglI PE=3 SV=1
Q5BB53	2.12e-106	1	662	1	823	Probable beta-glucosidase I OS=Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) OX=227321 GN=bglI PE=2 SV=2

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000063	0.000000	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

There is no transmembrane helices in MGYG000000628_00415.